BACKGROUND： Numerous studies have shown that magnetic resonance imaging （MRI） can detect survival and migration of super paramagnetic iron oxide-labeled stem cells in models of focal cerebral infarction. OBJECTIVE...BACKGROUND： Numerous studies have shown that magnetic resonance imaging （MRI） can detect survival and migration of super paramagnetic iron oxide-labeled stem cells in models of focal cerebral infarction. OBJECTIVE： To observe distribution of bone marrow mesenchymal stem cells （BMSCs） in a rat model of global brain ischemia following cardiac arrest and resuscitation, and to investigate the feasibility of tracing iron oxide-labeled BMSCs using non-invasive MRI. DESIGN, TIME AND SETTING： The randomized, controlled, molecular imaging study was performed at the Linbaixin Medical Research Center, Second Affiliated Hospital, Sun Yat-sen University, and the Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, China from October 2006 to February 2009. MATERIALS： A total of 40 clean, Sprague Dawley rats, aged 6 weeks and of either gender, were supplied by the Experimental Animal Center, Sun Yat-sen University, China, for isolation of BMSCs. Feridex （iron oxide）, Gyroscan Inetra 1.5T MRI system, and cardiopulmonary resuscitation device were used in this study. METHODS： A total of 30 healthy, male Sprague Dawiey rats, aged 6 months, were used to induce ventricular fibrillation using alternating current. After 8 minutes, the rats underwent 6-minute chest compression and mechanical ventilation, followed by electric defibrillation, to establish rat models of global brain ischemia due to cardiac arrest and resuscitation. A total of 24 successful models were randomly assigned to Feridex-labeled and non-labeled groups （n = 12 for each group）. At 2 hours after resuscitation, 5 ×10^8 Feridex-labeled BMSCs, with protamine sulfate as a carrier, and 5 ×10^6 non-labeled BMSCs were respectively transplanted into both groups of rats through the right carotid artery （cells were harvested in 1 mL phosphate buffered saline）. MAIN OUTCOME MEASURES： Feridex-labeled BMSCs were observed by Prussian blue staining and electron microscopy. Signal intensity, celluar viability, and proliferative capacity of BMSCs were measured using MRI, Trypan blue test, and M-IT assay, respectively. Distribution of transplanted cells was observed in rats utilizing MRI and Prussian blue staining prior to and 1, 3, 7, and 14 days after transplantation. RESULTS： Prussian blue staining displayed many blue granules in the Feridex-labeled BMSCs. High density of iron granules was observed in the cytoplasm under electron microscopy. According to MRI results, and compared with the non-labeled group, the signal intensity was decreased in the Feridex-labeled group （P 〈 0.05）. The decrease was most significant in the 50 pg/mL Feridex-labeled group （P 〈 0.01）. There were no significant differences in celluar viability and proliferation of BMSCs between the Feridex-labeled and non-labeled groups after 1 week （P 〉 0.05）. Low-signal lesions were detected in the rat hippocampus and temporal cortex at 3 days after transplantation. The low-signal lesions were still detectable at 14 days, and positively stained cells were observed in the hippocampus and temporal cortex using Prussian blue staining. There were no significant differences in signal intensity in the non-labeled group. CONCLUSION： BMSC transplantation traversed the blood-brain barrier and distributed into vulnerable zones in a rat model of cardiac arrest-induced global brain ischemia. MRI provided a non-invasive method to in vivo dynamically and spatially trace Feridex-labeled BMSCs after transplantation.展开更多
目的:观察黄芪扶正汤对子宫内膜癌宫腔镜电切术术后化疗患者血清中性粒细胞明胶酶相关性载脂蛋白(neutrophil gelatinase-associated lipocalin,NGAL)及其膜受体(neutrophil gelatinaseassociated lipocalin receptor,NGALR)与骨髓抑制...目的:观察黄芪扶正汤对子宫内膜癌宫腔镜电切术术后化疗患者血清中性粒细胞明胶酶相关性载脂蛋白(neutrophil gelatinase-associated lipocalin,NGAL)及其膜受体(neutrophil gelatinaseassociated lipocalin receptor,NGALR)与骨髓抑制的影响。方法:将57例患者随机分为对照组(28例)和观察组(29例),对照组于宫腔镜电切术术后静脉注射紫杉醇+顺铂治疗;观察组同时加服黄芪扶正汤,1剂/d。检测2组患者治疗前后血白细胞(WBC)、红细胞(RBC)、血小板计数(PLT)及免疫球蛋白A(Ig A)、免疫球蛋白M(Ig M)含量;采用酶联免疫吸附法检测患者血清NGAL及其受体NGALR蛋白表达水平;比较2组患者骨髓抑制、Karnofsky功能状态评分(Karnofsky Performance Status,KPS)及患者生存质量问卷(The Europe organization for Research and treatment of Cancer,Quality of life Questionnaire-C30,EORTC QLQ-C30)评分。结果:与对照组比较,观察组患者治疗后血WBC、RBC和PLT及免疫球蛋白Ig A、Ig M均升高(P<0.05);骨髓抑制分级中0、Ⅰ、Ⅱ度的患者例数增多,Ⅲ、Ⅳ度的患者例数均明显减少(P<0.05);血清NGAL及NGALR蛋白均低表达(P<0.05);KPS评分及EORTC QLQ-C30量表中躯体、情绪和社会功能等评分均升高(P<0.05)。结论:黄芪扶正汤可通过抑制NGAL与其受体NGALR结合而降低癌细胞侵袭及转移能力,还可减轻化疗引起的骨髓抑制,提高化疗效果。展开更多
基金the National Natural Science Foundation of China,No.30801081, 30870691,30700303the New Teacher Foundation of Doctor Center of Ministry of Education of China,No. 200805581179
文摘BACKGROUND： Numerous studies have shown that magnetic resonance imaging （MRI） can detect survival and migration of super paramagnetic iron oxide-labeled stem cells in models of focal cerebral infarction. OBJECTIVE： To observe distribution of bone marrow mesenchymal stem cells （BMSCs） in a rat model of global brain ischemia following cardiac arrest and resuscitation, and to investigate the feasibility of tracing iron oxide-labeled BMSCs using non-invasive MRI. DESIGN, TIME AND SETTING： The randomized, controlled, molecular imaging study was performed at the Linbaixin Medical Research Center, Second Affiliated Hospital, Sun Yat-sen University, and the Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, China from October 2006 to February 2009. MATERIALS： A total of 40 clean, Sprague Dawley rats, aged 6 weeks and of either gender, were supplied by the Experimental Animal Center, Sun Yat-sen University, China, for isolation of BMSCs. Feridex （iron oxide）, Gyroscan Inetra 1.5T MRI system, and cardiopulmonary resuscitation device were used in this study. METHODS： A total of 30 healthy, male Sprague Dawiey rats, aged 6 months, were used to induce ventricular fibrillation using alternating current. After 8 minutes, the rats underwent 6-minute chest compression and mechanical ventilation, followed by electric defibrillation, to establish rat models of global brain ischemia due to cardiac arrest and resuscitation. A total of 24 successful models were randomly assigned to Feridex-labeled and non-labeled groups （n = 12 for each group）. At 2 hours after resuscitation, 5 ×10^8 Feridex-labeled BMSCs, with protamine sulfate as a carrier, and 5 ×10^6 non-labeled BMSCs were respectively transplanted into both groups of rats through the right carotid artery （cells were harvested in 1 mL phosphate buffered saline）. MAIN OUTCOME MEASURES： Feridex-labeled BMSCs were observed by Prussian blue staining and electron microscopy. Signal intensity, celluar viability, and proliferative capacity of BMSCs were measured using MRI, Trypan blue test, and M-IT assay, respectively. Distribution of transplanted cells was observed in rats utilizing MRI and Prussian blue staining prior to and 1, 3, 7, and 14 days after transplantation. RESULTS： Prussian blue staining displayed many blue granules in the Feridex-labeled BMSCs. High density of iron granules was observed in the cytoplasm under electron microscopy. According to MRI results, and compared with the non-labeled group, the signal intensity was decreased in the Feridex-labeled group （P 〈 0.05）. The decrease was most significant in the 50 pg/mL Feridex-labeled group （P 〈 0.01）. There were no significant differences in celluar viability and proliferation of BMSCs between the Feridex-labeled and non-labeled groups after 1 week （P 〉 0.05）. Low-signal lesions were detected in the rat hippocampus and temporal cortex at 3 days after transplantation. The low-signal lesions were still detectable at 14 days, and positively stained cells were observed in the hippocampus and temporal cortex using Prussian blue staining. There were no significant differences in signal intensity in the non-labeled group. CONCLUSION： BMSC transplantation traversed the blood-brain barrier and distributed into vulnerable zones in a rat model of cardiac arrest-induced global brain ischemia. MRI provided a non-invasive method to in vivo dynamically and spatially trace Feridex-labeled BMSCs after transplantation.
文摘目的:观察黄芪扶正汤对子宫内膜癌宫腔镜电切术术后化疗患者血清中性粒细胞明胶酶相关性载脂蛋白(neutrophil gelatinase-associated lipocalin,NGAL)及其膜受体(neutrophil gelatinaseassociated lipocalin receptor,NGALR)与骨髓抑制的影响。方法:将57例患者随机分为对照组(28例)和观察组(29例),对照组于宫腔镜电切术术后静脉注射紫杉醇+顺铂治疗;观察组同时加服黄芪扶正汤,1剂/d。检测2组患者治疗前后血白细胞(WBC)、红细胞(RBC)、血小板计数(PLT)及免疫球蛋白A(Ig A)、免疫球蛋白M(Ig M)含量;采用酶联免疫吸附法检测患者血清NGAL及其受体NGALR蛋白表达水平;比较2组患者骨髓抑制、Karnofsky功能状态评分(Karnofsky Performance Status,KPS)及患者生存质量问卷(The Europe organization for Research and treatment of Cancer,Quality of life Questionnaire-C30,EORTC QLQ-C30)评分。结果:与对照组比较,观察组患者治疗后血WBC、RBC和PLT及免疫球蛋白Ig A、Ig M均升高(P<0.05);骨髓抑制分级中0、Ⅰ、Ⅱ度的患者例数增多,Ⅲ、Ⅳ度的患者例数均明显减少(P<0.05);血清NGAL及NGALR蛋白均低表达(P<0.05);KPS评分及EORTC QLQ-C30量表中躯体、情绪和社会功能等评分均升高(P<0.05)。结论:黄芪扶正汤可通过抑制NGAL与其受体NGALR结合而降低癌细胞侵袭及转移能力,还可减轻化疗引起的骨髓抑制,提高化疗效果。
