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Effects of granulocyte colony-stimulating factor and stem cell factor, alone and in combination, on the biological behaviours of bone marrow mesenchymal stem cells
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作者 Feng-Ping Tang Xing-Huo Wu +3 位作者 Xi-Lin Yu Shu-Hua Yang Wei-Hua Xu Jin Li 《Journal of Biomedical Science and Engineering》 2009年第3期200-207,共8页
Aim: The effects of granulocyte colony- stimu-lating factor (G-CSF) and stem cell factor (SCF) on the proliferation and osteogenic differentia-tion capacity of bone marrow mesenchymal stem cells (MSCs) were studied in... Aim: The effects of granulocyte colony- stimu-lating factor (G-CSF) and stem cell factor (SCF) on the proliferation and osteogenic differentia-tion capacity of bone marrow mesenchymal stem cells (MSCs) were studied in the experi-ment. Methods: Bone marrow MSCs were col-lected from rabbits successfully, and treated with various concentrations of G-CSF, SCF or a combination of the two. Flow cytometric ana-lyse, MTT test, CFU-F assay, and alkaline phosphatase (ALP) activity measurement were employed. Results: The results of flow cytome-try showed that immunophenotype of the cells were CD29+/CD45-, CD105+/ CD34–, CD90+/ HLADR–. MSCs were shown to constitutively express low levels of c-kit which could be en-hanced by SCF. G-CSF and SCF had an obvious facilitative effect on the proliferation of MSCs in a dose-dependent fashion. In addition, G-CSF and SCF would be effective in reversibly pre-venting their differentiation, as showed by the decrease of ALP activity, leading to self-renewal rather than differentiative cell divisions. The effects of G-CSF were superior to SCF. And cells in the group treated with combination of G-CSF and SCF showed more powerful effects than the groups treated with G-CS, SCF, or none of the two. Conclusion: On the whole, these studies demonstrated that MSCs responsed to G-CSF, SCF, and to G-CSF plus SCF in a manner that suppressed differentiation, and promotes proliferation and self-renewal, and support the view that these factors could act synergistically. 展开更多
关键词 GRANULOCYTE Colony-Stimulating FACTOR STEM Cell FACTOR Synergistic effect bone marrow MESENCHYMAL STEM Cells
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Synergistic effects of brain-derived neurotrophic factor and retinoic acid on inducing the differentiation of bone marrow stromal cells into neuron-like cells in adult rats in vitro
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作者 Yonghai Liu Yucheng Song Zunsheng Zhang Xia Shen 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第4期301-303,共3页
BACKGROUND: Under induction of retinoic acid (RA), bone marrow stromal cells (BMSCs) can differentiate into nerve cells or neuron-like cells, which do not survive for a long time, so those are restricted to an ap... BACKGROUND: Under induction of retinoic acid (RA), bone marrow stromal cells (BMSCs) can differentiate into nerve cells or neuron-like cells, which do not survive for a long time, so those are restricted to an application. Other neurotrophic factors can also differentiate into neuronal cells through inducing BMSCs; especially, brain-derived neurotrophic factor (BDNF) can delay natural death of neurons and play a key role in survival and growth of neurons. The combination of them is beneficial for differentiation of BMSCs. OBJECTIVE: To investigate the effects of BDNF combining with RA on inducing differentiation of BMSCs to nerve cells of adult rats and compare the results between common medium group and single BDNF group. DESIGN: Randomized controlled animal study SETTING: Department of Neurology, Affiliated Hospital of Xuzhou Medical College MATERIALS: The experiment was carried out in the Clinical Neurological Laboratory of Xuzhou Medical College from September 2003 to April 2005. A total of 24 SD rats, of either gender, 2 months old, weighing 130-150 g, were provided by Experimental Animal Center of Xuzhou Medical College [certification: SYXK (su) 2002-0038]. Materials and reagents: low-glucose DMEM medium, bovine serum, BDNF, RA, trypsin, separating medium of lymphocyte, monoclonal antibody of mouse-anti-nestin, neuro-specific enolase, glial fibrillary acidic protein (GFAP) antibody, SABC kit, and diaminobenzidine (DAB) color agent. All these mentioned above were mainly provided by SIGMA Company, GIBCO Company and Boshide Company. METHODS: Bone marrow of SD rats was selected for density gradient centrifugation. BMSCs were undertaken primary culture and subculture; and then, those cells were induced respectively in various mediums in total of 3 groups, including control group (primary culture), BDNF group (20 μg/L BDNF) and BDNF+RA group (20 μg/L BDNF plus 20 μg/L RA). On the 3^rd and the 7^th days after induction, BMSCs were stained immunocytochemically with nestin (sign of nerve stem cells), neuron-specific enolase (NSE, sign of diagnosing neurons) and GFAP (diagnosing astrocyte), and evaluated cellular property. MAIN OUTCOME MEASURES : Induction and differentiation in vitro of BMSCs in 3 groups RESULTS: (1) Induction and differentiation of BMSCs: Seven days after induction, cells having 2 or more apophyses were observed. Soma shaped like angle or erose form, which were similar to neurons and glial cells having strong refraction. (2) Results of immunocytochemical detection: Three days after induction, rate of positive cells in BDNF+RA group was higher than that in BDNF group and control group [(86.15±4.58)%, (65.43±4.23)%, (4.18±1.09)%, P 〈 0.01]. Seven days after induction, rate of positive cells was lower in BDNF group and BDNF+RA group than that in both groups at 3 days after induction [(31.12±3.18)%, (29.35±2.69)%, P 〈 0.01]; however, amounts of positive cells of NSE and GFAP were higher than those at 3 days after induction (P 〈 0.01); meanwhile, the amount in BDNF+RA group was remarkably higher than that in BDNF group (P 〈 0.01). CONCLUSION: Combination of BDNF and RA can cooperate differentiation of BMSCs into neurons and astrocyte, and the effect is superior to single usage of BDNF. 展开更多
关键词 cell bone Synergistic effects of brain-derived neurotrophic factor and retinoic acid on inducing the differentiation of bone marrow stromal cells into neuron-like cells in adult rats in vitro BMSCS BDNF acid
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THE EARLY RELATIVE BIOLOGICAL EFFECTIVENESS OF SINGLE DOSE OF FAST NEUTRONS(35MeV_(p→Be)) FOR BONE MARROW IN MICE
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作者 孙艳 韩树奎 +4 位作者 徐博 张淑慧 唐景华 常维克 申文江 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1995年第1期71-74,共4页
The early RBE of the bone marrow in mice after studied irradiation with fast neutrons(35 MeVp→Be) was studied.60Co-γ ray was used for referent beams.Using the dos.making 50% loss of the nucleated cells of bone marro... The early RBE of the bone marrow in mice after studied irradiation with fast neutrons(35 MeVp→Be) was studied.60Co-γ ray was used for referent beams.Using the dos.making 50% loss of the nucleated cells of bone marrow in mice relative to control group mice to calculated the RBE value which was 2.13±0.18.Meanwhile,the relationship of the RBE values and the dose of neutrons was noted.On log-log plot the RBE values decrease with increasing dose of fast neutrons and it is consistent with a slope of -0.39± 0.10.The α/β ratios were estimated from linear-quadratic model of cell survival,they are 14.4±1.30 Gy for fast neutrons and 0.83±0.10 Gy for γ-ray,respectively. 展开更多
关键词 Fast neutrons Relative biological effectiveness bone marrow Early reaction.
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Effects of chitosan on dental bone repair
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作者 Fatemeh Ezoddini-Ardakani Alireza Navab Azam +2 位作者 Soghra Yassaei Farhad Fatehi Gholamreza Rouhi 《Health》 2011年第4期200-205,共6页
Objectives: Bone defects following tumor resection and osteolysis due to dental and bone lesions and periodentium tissue disorders are serious challenges. One of these materials used is chitosan, a derivative of crust... Objectives: Bone defects following tumor resection and osteolysis due to dental and bone lesions and periodentium tissue disorders are serious challenges. One of these materials used is chitosan, a derivative of crustaceans’ exoskeleton. The aim of this study was to assess effects of chitosan on socket repair after dental extraction. Methods: Twenty four dental sockets of 15-24 years old patients were visited by a maxillofacial surgeon for extracting premolar teeth for orthodontic purposes. The sockets in one side were filled-in by chitosan. In the other side, the sockets were left unfilled. After 10 weeks, periapical radiographs were obtained from the repair sites, were digitalized and then evaluated for densitometry using Adobe Photoshop Software. Each socket was divided into coronal, middle and apical. Dental density of each socket in case and control groups was recorded. The density of regenerated bone was compared against the maximum bone density of each individual. Wilcoxon signed range test and paired t-test were used for data analysis. Results: Bone density in middle and apical sections in case group was significantly more than control group. In apical section in case group regenerated bone reached up to 98.2% of normal bone density. In each patient, the bone density in epical and middle sections was increase 29.3% and 10.8% of normal bone density. Conclusions: Chitosan significantly increased bone density in epical and middle sections. Chitosan can be used for bone repair in cases of bone loss. Various densitometry studies for evaluating chitosan effects in different bone defects are suggested. 展开更多
关键词 CHITOSAN bone Regeneration/drug effects BIOCOMPATIBLE Materials/Administration & DOSAGE
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Cost Effectiveness Analysis of Filgrastim versus Placebo in Post AIIogentic Bone Marrow Transplantation
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作者 Maoudoud Ines Razgallah Khrouf Myriam +8 位作者 Ben Abdejelil Nour Ghedira Hela Amel Lakhal Marsit Hanene Turki Manel Soussi Mohamed Ali Lazreg Olfa Ladab Saloua Ben Othmane Tarek 《Journal of Pharmacy and Pharmacology》 2016年第6期268-272,共5页
Filgrastim is used to accelerate hematopoietic recovery after ABMT (allogeneic bone marrow transplantation). Its impact on the total cost of patient care remains to be explored. We therefore undertook a cost effecti... Filgrastim is used to accelerate hematopoietic recovery after ABMT (allogeneic bone marrow transplantation). Its impact on the total cost of patient care remains to be explored. We therefore undertook a cost effectiveness analysis in the context of a randomized single blinded clinical trial of Filgrastim versus placebo in post ABMT. A primary endpoint, duration of myelosuppression, and three secondary end points (number of days of fever, length of hospital stay, survival at one hundred days) were used to assess efficacy. Direct costs were evaluated and allowed the calculation of the ICER (incremental cost-effectiveness ratios) for the major endpoint of the trial. Sixteen patients were included in the study. The duration of myelosuppression was significantly decreased in the Filgrastim arm with medians of 15 days vs. 19 days in the placebo arm (p = 0.023). Cost analysis showed no statistically significant difference between the two arms. According to the calculation of ICER, Filgrastim was more costly and more effective than placebo for the number of days of aplasia avoided and the number of days with fever avoided. Placebo strictly dominated filgrastim for days of hospitalization avoided. Filgrastim has proven effective in reducing the duration of aplasia without increasing costs. 展开更多
关键词 FILGRASTIM PLACEBO COST effectIVENESS allogeneic bone marrow transplantation.
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Sustained mutagenic effect in bone marrow cells induced by signal nuclide ^(134)Cs retention in skeleton
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作者 Zhu Shou Peng, Xia Fenand Yang Wei Dong ( Suzhou Medical College, Suzhou 215007) 《Nuclear Science and Techniques》 SCIE CAS CSCD 1997年第1期56-58,共3页
Sustainedmutagenicefectinbonemarrowcelsinducedbysignalnuclide134CsretentioninskeletonZhuShouPeng,XiaFenandYa... Sustainedmutagenicefectinbonemarrowcelsinducedbysignalnuclide134CsretentioninskeletonZhuShouPeng,XiaFenandYangWeiDong(Suzh... 展开更多
关键词 骨髓细胞 致变物效应 134Cs
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Effect of Ligustrazine on Expressions of Basic Fibroblast Growth Factor and Its Receptor in Bone Marrow of Mice with Acute Radiation Injury
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作者 吴宁 孙汉英 +5 位作者 刘文励 孟凡凯 刘振芳 徐慧珍 路武 谢瑶 《Chinese Journal of Integrated Traditional and Western Medicine》 2004年第3期225-225,共1页
Objective: To study the expressions of basic fibroblast growth factor (bFGF) and its receptor (bFGFR) in bone marrow of mice with acute radiation injury, and to evaluate the effect of Ligustrazine (Lt) on them. Method... Objective: To study the expressions of basic fibroblast growth factor (bFGF) and its receptor (bFGFR) in bone marrow of mice with acute radiation injury, and to evaluate the effect of Ligustrazine (Lt) on them. Methods: Fifty-six Kunming mice of clean grade were randomly divided into 3 groups, the normal group, the control group and the Lt group. Mice in the latter two groups were once homogeneously systemic irradiated with 6.0 Gy of 60 Co, with the absorption dose rate of 0. 56 Gy/min, then treated with saline (0.2 ml/ mice) or Lt (2 mg/mice) respectively, twice a day through gastrogavage for successive 13 days. Mice were sacrificed in batch on the 3rd, 7th and 14th day by cervical dislocation to collect the bilateral femoral bone marrow for preparing bone marrow mono-nuclear cell (BMMNC) suspension. The bFGFR expression on surface of BMMNC was determined by flow cytometry; and the bFGF expres-sion level in one side of femoral bone marrow tissue was detected by immunohistochemistry with SABC-AP assay. Results: The bFGF expression in bone marrow of mice on the 3rd, 7th and 14th day after acute radiation injury all were significantly lower than that of the normal mice (P<0.05 or P<0.01). The expressions of bFGF and bFGFR in the Lt group detected were significantly higher than that in the control group detected at the corresponding time points (P<0.05 or P < 0.01). Conclusion:By way of enhancing bFGF expression in bone marrow and bFGFR expression on surface of BMMNC to accelerate the repairing of hemopoietic micro-environment in bone marrow might be one of the mechanisms of Lt in promoting hemopoietic function reconstitution after acute radiation injury.Original article on CJITWM (Chin) 2004;24(5):439 展开更多
关键词 effect of Ligustrazine on Expressions of Basic Fibroblast Growth Factor and Its Receptor in bone marrow of Mice with Acute Radiation Injury
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The effect of neuropeptides on proliferation of rat bone marrow mesenchymal stem cells
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作者 王钊 《外科研究与新技术》 2011年第2期128-129,共2页
Objective To investigate the effects and mechanism of calcitonin gene-related peptide(CGRP)and substance P (SP) on proliferation of rat bone marrow mesenchymal stem cells.Methods The rBMSCs were isolated using whole b... Objective To investigate the effects and mechanism of calcitonin gene-related peptide(CGRP)and substance P (SP) on proliferation of rat bone marrow mesenchymal stem cells.Methods The rBMSCs were isolated using whole bone marrow 展开更多
关键词 bone SP BMSCS The effect of neuropeptides on proliferation of rat bone marrow mesenchymal stem cells STEM
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Updates in the pathophysiological mechanisms of Parkinson's disease: Emerging role of bone marrow mesenchymal stem cells 被引量:9
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作者 Hanaa H Ahmed Ahmed M Salem +5 位作者 Hazem M Atta Emad F Eskandar Abdel Razik H Farrag Mohamed A Ghazy Neveen A Salem Hadeer A Aglan 《World Journal of Stem Cells》 SCIE CAS 2016年第3期106-117,共12页
AIM: To explore the approaches exerted by mesenchymal stem cells (MSCs) to improve Parkinson&#x02019;s disease (PD) pathophysiology.METHODS: MSCs were harvested from bone marrow of femoral bones of male rats, grow... AIM: To explore the approaches exerted by mesenchymal stem cells (MSCs) to improve Parkinson&#x02019;s disease (PD) pathophysiology.METHODS: MSCs were harvested from bone marrow of femoral bones of male rats, grown and propagated in culture. Twenty four ovariectomized animals were classified into 3 groups: Group (1) was control, Groups (2) and (3) were subcutaneously administered with rotenone for 14 d after one month of ovariectomy for induction of PD. Then, Group (2) was left untreated, while Group (3) was treated with single intravenous dose of bone marrow derived MSCs (BM-MSCs). SRY gene was assessed by PCR in brain tissue of the female rats. Serum transforming growth factor beta-1 (TGF-&#x003b2;1), monocyte chemoattractant protein-1 (MCP-1) and brain derived neurotrophic factor (BDNF) levels were assayed by ELISA. Brain dopamine DA level was assayed fluorometrically, while brain tyrosine hydroxylase (TH) and nestin gene expression were detected by semi-quantitative real time PCR. Brain survivin expression was determined by immunohistochemical procedure. Histopathological investigation of brain tissues was also done.RESULTS: BM-MSCs were able to home at the injured brains and elicited significant decrease in serum TGF-&#x003b2;1 (489.7 &#x000b1; 13.0 vs 691.2 &#x000b1; 8.0, P &#x0003c; 0.05) and MCP-1 (89.6 &#x000b1; 2.0 vs 112.1 &#x000b1; 1.9, P &#x0003c; 0.05) levels associated with significant increase in serum BDNF (3663 &#x000b1; 17.8 vs 2905 &#x000b1; 72.9, P &#x0003c; 0.05) and brain DA (874 &#x000b1; 15.0 vs 599 &#x000b1; 9.8, P &#x0003c; 0.05) levels as well as brain TH (1.18 &#x000b1; 0.004 vs 0.54 &#x000b1; 0.009, P &#x0003c; 0.05) and nestin (1.29 &#x000b1; 0.005 vs 0.67 &#x000b1; 0.006, P &#x0003c; 0.05) genes expression levels. In addition to, producing insignificant increase in the number of positive cells for survivin (293.2 &#x000b1; 15.9 vs 271.5 &#x000b1; 15.9, P &#x0003e; 0.05) expression. Finally, the brain sections showed intact histological structure of the striatum as a result of treatment with BM-MSCs.CONCLUSION: The current study sheds light on the therapeutic potential of BM-MSCs against PD pathophysiology via multi-mechanistic actions. 展开更多
关键词 Parkinson’s disease PATHOPHYSIOLOGY bone marrow derived mesenchymal stem cells ROTENONE Anti-inflammatory action OVARIECTOMY Anti-apoptotic effect Neurogenic potential
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Preliminary delivery efficiency prediction of nanotherapeutics into crucial cell populations in bone marrow niche 被引量:1
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作者 Huijuan Chen Anzhi Hu +6 位作者 Mengdi Xiao Shiyi Hong Jing Liang Quanlong Zhang Yang Xiong Mancang Gu Chaofeng Mu 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2023年第6期113-125,共13页
Several crucial stromal cell populations regulate hematopoiesis and malignant diseases in bone marrow niches.Precise regulation of these cell types can remodel niches and develop new therapeutics.Multiple nanocarriers... Several crucial stromal cell populations regulate hematopoiesis and malignant diseases in bone marrow niches.Precise regulation of these cell types can remodel niches and develop new therapeutics.Multiple nanocarriers have been developed to transport drugs into the bone marrow selectively.However,the delivery efficiency of these nanotherapeutics into crucial niche cells is still unknown,and there is no method available for predicting delivery efficiency in these cell types.Here,we constructed a three-dimensional bone marrow niche composed of three crucial cell populations:endothelial cells(ECs),mesenchymal stromal cells(MSCs),and osteoblasts(OBs).Mimetic niches were used to detect the cellular uptake of three typical drug nanocarriers into ECs/MSCs/OBs in vitro.Less than 5%of nanocarriers were taken up by three stromal cell types,and most of themwere located in the extracellular matrix.Delivery efficiency in sinusoidal ECs,arteriole ECs,MSCs,and OBs in vivo was analyzed.The correlation analysis showed that the cellular uptake of three nanocarriers in crucial cell types in vitro is positively linear correlated with its delivery efficiency in vivo.The delivery efficiency into MSCs was remarkably higher than that into ECs and OBs,no matterwhat kind of nanocarrier.The overall efficiency into sinusoidal ECswas greatly lower than that into arteriole ECs.All nanocarriers were hard to be delivered into OBs(<1%).Our findings revealed that cell tropisms of nanocarriers with different compositions and ligand attachments in vivo could be predicted via detecting their cellular uptake in bone marrow niches in vitro.This study provided the methodology for niche-directed nanotherapeutics development. 展开更多
关键词 bone marrow niche mimicking drug delivery prediction Nanotherapeutics bone marrow stromal cells
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Study on Oxygen Supply and Protection of Bone Marrow in Acute Radiation injured Mice
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作者 孙汉英 刘文励 +5 位作者 肖侃艳 董凌莉 何美冬 胡永熙 沈安华 江琦 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1997年第4期229-231,243,共4页
After irradiated by & Gy 60Co γ-ray, mice were intraperitoneally injected immediately with 0.2 ml 100 % compound blood-activating soup twice a day for 10 days. The in situ ulnar bone marrow partial pressure of ox... After irradiated by & Gy 60Co γ-ray, mice were intraperitoneally injected immediately with 0.2 ml 100 % compound blood-activating soup twice a day for 10 days. The in situ ulnar bone marrow partial pressure of oxygen (PbO2) was determined in vivo before, during and after irradiation respectively. The bone marrow sections in the same part were observed. Our results showed that the normal murine ulnar PbO2 was 12.72±1. 05kpa. During irradiation, the level of PbO2 decreased to 10. 78±1. 17 kpa (P<0. 001). And 3 days after irradiation, PbO2 decreased to 9. 75±0. 52 kpa, suggesting that the commonly used 'blood-activating and stasis-eliminating' Chinese drugs could promote the rehabilitation and proliferation of bone marrow microvessels in the acute radiation injured mice, expand their areas, increase the oxygen supply of bone marrow microenviroment, thereby leading to PbO2 much higher increase than that of control group. It is also helpful in the proliferation and rehabilitation of hematopoietic cells. 展开更多
关键词 acute radiation injury bone marrow microenviroment partial Pressure of oxygen 'blood-activating and stasis-eliminating'Chinese drug
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An Enzymologic Study on Bone Marrow Cells in Patients with Aplastic Anemia Treated by Supplementing the Kidney and Removing Blood Stasis
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作者 王树庆 刘捷 +3 位作者 李建华 张圣明 胡匡祜 李子孝 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第3期169-171,共3页
  The content of cytochrome oxidase (CCO), succinate dehydrogenase (SDH) and neutrophil alkaline phosphatase (NAP) in bone marrow cells in 68 cases of aplastic anemia before and after treatment was determined by com...   The content of cytochrome oxidase (CCO), succinate dehydrogenase (SDH) and neutrophil alkaline phosphatase (NAP) in bone marrow cells in 68 cases of aplastic anemia before and after treatment was determined by computerized graphical analysis and compared with that of normal volunteers (control group). The significantly lowered CCO and SDH levels and the markedly increased NAP content before treatment (P<0.01) became approximately normal after that of supplementing the kidney and removing blood stasis (P >0.05). 展开更多
关键词 ADOLESCENT Adult Alkaline Phosphatase Anemia Aplastic bone marrow Cells CHILD Child Preschool drugs Chinese Herbal Electron Transport Complex IV Female Humans Male Middle Aged Succinate Dehydrogenase
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外泌体miRNA治疗多发性骨髓瘤的作用与机制
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作者 赵艺涵 孙旭杭 +1 位作者 赵琳 蒋士卿 《中国组织工程研究》 CAS 北大核心 2025年第31期6743-6752,共10页
背景:多发性骨髓瘤是最常见的血液恶性肿瘤之一,具有难治疗、易复发、易耐药的特点,至今仍无法治愈。外泌体的内源性转运系统通过影响细胞间功能成分的交换而发挥通讯作用,其中miRNA因尺寸微小更容易被包装在外泌体内,故影响细胞功能的... 背景:多发性骨髓瘤是最常见的血液恶性肿瘤之一,具有难治疗、易复发、易耐药的特点,至今仍无法治愈。外泌体的内源性转运系统通过影响细胞间功能成分的交换而发挥通讯作用,其中miRNA因尺寸微小更容易被包装在外泌体内,故影响细胞功能的途径多、范围广。目的:归纳并总结多发性骨髓瘤中外泌体miRNA发挥作用的机制,提出潜在靶点。方法:以“multiple myeloma,bone marrow mesenchyml stem cell,fibroblasts,peripheral blood,complication,progression,drug resistance,exosomal miRNA”为英文关键检索词,检索PubMed数据库的相关文献。文献检索时限为数据库建库至2024年6月。通过阅读文题和摘要进行初筛,排除无关或内容重复或无参考意义的文献,最后纳入81篇文献进行综述。结果与结论:①多发性骨髓瘤细胞通过各种miRNA介导的外泌体调节成骨和破骨功能平衡参与多发性骨髓瘤骨病变、调控红细胞钙离子外排通道参与高钙血症的发生、促进肾上皮-间充质转化引发肾纤维化和肾功能不全、上调骨髓来源的单核抑制细胞促进免疫微环境的抑制;此外,多发性骨髓瘤细胞外泌体miRNA还参与了免疫治疗耐药的发生和发展以及血管生成、细胞增殖及细胞衰老等病理过程。②骨髓间充质干细胞和肿瘤相关成纤维细胞均可通过外泌体miRNA影响多发性骨髓瘤细胞的增殖、转移和凋亡,干预血管生成,调控化疗药物的耐药性。③外周血循环外泌体miRNA作为前沿的无创生物标志物,可发挥判断多发性骨髓瘤病程分期和进展,预测患者生存及预后,评估药物敏感性和耐药性等关键作用。④多发性骨髓瘤外泌体长链非编码RNA、环状RNA、miRNA及mRNA,靶基因多种研究因素可形成不同的组合,灵活地构建新型生物轴和生物分子网络,符合整体医学的发展理念。⑤外泌体miRNA作为预测生物标志物及新药研发的治疗靶点具有巨大的发展潜力,如何最大程度利用外泌体miRNA,如何加快相关科研结果的临床转化值得未来进一步研究。 展开更多
关键词 多发性骨髓瘤 进展 耐药 并发症 骨髓间充质干细胞 成纤维细胞 外周血 外泌体 MIRNA
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Graft-versus-leukemia effects from donor lymphocyte infusion after nonmyeloablative allogeneic bone marrow transplantation in mice 被引量:2
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作者 DUBing LIDe-peng XUKai-lin PANXiu-ying 《Chinese Medical Journal》 SCIE CAS CSCD 2005年第6期474-479,共6页
BABSTRACTackground Nonmyeloablative allogeneic bone marrow transplantation has been used since the 1990s as a new hematological stem cell transplantation strategy for treating hematological diseases. The purpose of th... BABSTRACTackground Nonmyeloablative allogeneic bone marrow transplantation has been used since the 1990s as a new hematological stem cell transplantation strategy for treating hematological diseases. The purpose of this study was to explore the graft-versus-leukemia (GVL) effects of donor lymphocyte infusions (DLIs) after nonmyeloablative allogeneic bone marrow transplantations, while assessing the declines in treatment-associated morbidity, mortality, and graft-versus-host disease (GVHD).Methods A total of 615 (H-2~k) mice were injected with L615 tumor cells and received 500 cGy (^(60)Coγ-ray) irradiation three days later, followed by an allogeneic bone marrow transplantation (allo-BMT). The allo-grafts consisted of 3×10~7 bone marrow cells and 1×10~7 spleen cells from BALB/C (H-2~d) donor mice. Two days after the allo-BMT, the recipient mice were given 200 mg/kg of cyclophosphamide. Subsequently, recipient mice were infused with either donor spleen cells (2×10~7) on day 14 or 21, or donor spleen cells (5×10~7) pretreated with hydrocortisone and cyclosporin A (CsA) in vitro on day 14 post-BMT.Results The median survival time of mice that received DLI on day 21 and pretreated DLI on day 14 post-BMT was longer than that of controls and the day 14 DLI group (P<0.01). No evidence of severe GVHD was observed in the day 21 DLI group nor in the day 14 treated DLI group. Mixed chimerism was confirmed in the day 14 DLI group, the day 14 treated DLI group, and the day 21 DLI group on the thirteenth day post-transplantation; full donor chimerism was observed two weeks after DLI.Conclusion Donor lymphocyte infusion after nonmyeloablative bone marrow transplantation may reduce transplantation-associated morbidity and mortality while strengthening graft-versus-leukemia effects. 展开更多
关键词 bone marrow transplantation NONMYELOABLATIVE LYMPHOCYTE DONOR graft versus host disease graft versus leukemia effect
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Radiogenotoxicological effect of signal nuclide ^(134)Cs on somatic and germ cells 被引量:1
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作者 Zhu Shou Peng, Xia Fenand Yang Wei Dong ( Suzhou Medical College, Suzhou 215007) 《Nuclear Science and Techniques》 SCIE CAS CSCD 1997年第1期59-61,共3页
Radiogenotoxicologicalefectofsignalnuclide134CsonsomaticandgermcelsZhuShouPeng,XiaFenandYangWeiDong(SuzhouMe... Radiogenotoxicologicalefectofsignalnuclide134CsonsomaticandgermcelsZhuShouPeng,XiaFenandYangWeiDong(SuzhouMedicalColege,Su... 展开更多
关键词 人体细胞 细菌细胞 核素^134Cs 辐照损伤
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麝香黄芪复方滴丸含药血清促进骨髓间充质干细胞增殖分化 被引量:1
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作者 陈娜 王燕琳 +3 位作者 孙慧芳 樊飞燕 李东红 张运克 《中国组织工程研究》 CAS 北大核心 2024年第19期2960-2966,共7页
背景:骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)已被广泛用于治疗神经系统疾病,但由于血脑屏障的限制以及干细胞在受损部位存活率、分化率低,导致治疗效果有限。目的:探讨麝香黄芪复方滴丸含药血清对BMSCs增殖、迁... 背景:骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)已被广泛用于治疗神经系统疾病,但由于血脑屏障的限制以及干细胞在受损部位存活率、分化率低,导致治疗效果有限。目的:探讨麝香黄芪复方滴丸含药血清对BMSCs增殖、迁移和向星形胶质细胞分化的影响。方法:SD雄性大鼠连续灌胃麝香黄芪复方滴丸5 d后进行腹主动脉取血,分离血清备用。采用CCK-8法检测体积分数5%,10%,20%含药血清对BMSCs增殖的影响;划痕实验观察体积分数10%含药血清对BMSCs横向迁移的影响;Transwell小室培养BMSCs,用结晶紫染色和DAPI核染色观察体积分数10%含药血清对BMSCs纵向迁移的影响;用含体积分数10%含药血清的诱导液或与星形胶质细胞共培养观察BMSCs向星形胶质细胞分化情况。结果与结论:①体积分数10%和20%含药血清在第2,3天促进细胞增殖更明显,且两种体积分数无统计学差异;②在划痕30,48 h时,10%含药血清组BMSCs迁移量显著高于对照组;③10%含药血清组BMSCs穿过Transwell小室滤过膜的数量高于对照组;④体积分数10%含药血清可能促进BMSCs向星形胶质细胞方向分化,但分化作用较弱,星形胶质细胞能进一步促进含药血清诱导BMSCs向星形胶质细胞方向分化;⑤结果表明,体积分数10%含药血清能促进BMSCs体外增殖、迁移;与星形胶质细胞共培养可能促进BMSCs向星形胶质细胞方向分化。 展开更多
关键词 缺血性脑卒中 麝香黄芪复方滴丸 含药血清 骨髓间充质干细胞 星形胶质细胞 增殖 迁移 分化
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中医药联合医用水凝胶治疗疾病的策略及意义 被引量:2
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作者 毕玉杰 马笃军 +5 位作者 彭力平 周紫琼 赵静 朱厚均 钟秋辉 杨玉鑫 《中国组织工程研究》 CAS 北大核心 2024年第3期419-425,共7页
背景:医用水凝胶是具有三维结构网络的新型功能高分子材料,具有出色的生物相容性,目前已在组织工程领域、药物载体领域有广泛研究,但基于组织工程探究医用水凝胶与中医药结合治疗疾病的研究还处于初期探索阶段。因此,通过对医用水凝胶... 背景:医用水凝胶是具有三维结构网络的新型功能高分子材料,具有出色的生物相容性,目前已在组织工程领域、药物载体领域有广泛研究,但基于组织工程探究医用水凝胶与中医药结合治疗疾病的研究还处于初期探索阶段。因此,通过对医用水凝胶机制作用的剖析,整合医用水凝胶与中医药在研究中联合应用的文章,进而更好地为科研工作者提供思路,对中医药与医用水凝胶联合应用具有重要意义。目的:基于组织工程研究探讨中医药联合医用水凝胶治疗疾病的策略及意义。方法:利用PubMed和中国知网数据库,检索有关中医药联合医用水凝胶在组织工程中应用的文献,检索时间为2010年1月至2022年11月,英文检索词为“hydrogel,traditional Chinese medicine,drug carrier,tissue engineering”,中文检索词为“医用水凝胶、中医药、药物载体、组织工程”。根据纳入与排除标准对所有文章进行初筛后,最终纳入61篇文章进行综述。结果与结论:①中医药联合医用水凝胶的应用虽然在关节内、组织器官内、软组织伤口和组织工程等方面有所涉及,但除了中医药结合水凝胶敷料在临床应用治疗软组织损伤外,其他方面尚处于基础实验阶段。②中医药联合医用水凝胶的发展有着巨大潜力和发展前景,但对于性能要求较高的凝胶在制造方面存在一定难度,理化性质精确掌握难度较大。③目前综合来看可注射水凝胶凭借着简便易用的特点,其在与中医药联合使用可延伸范围较广,可用于关节、器官和组织工程相关疾病的治疗;智能水凝胶有较高的灵敏度和可逆转化性也可满足特殊环境下的使用;将两者结合的中医药使用过程中还需要明确中药成分的作用机制。④中医药联合医用水凝胶治疗疾病的策略应着手于中医药对器官、组织、细胞的治疗作用联合适当种类的医用水凝胶进行匹配,可弥补传统中医给药方式和频繁给药的不足,在组织工程方面可以用水凝胶负载中药干预后的干细胞,或者同时负载中药和干细胞用于相关疾病的治疗。⑤在中医药联合医用水凝胶应用的未来研究中,还需要考虑:应当确保医用水凝胶生物性能可以量化,以不同材料不同制造工艺把握水凝胶特性,制造出所需要的符合应用条件的医用水凝胶;在中医药方面需要对已知中药单体、中药复方提取物的治疗效果和应用机制全面了解剖析,在更明了的机制下实现中医药与医用水凝胶更多更完美的结合;借助医学科技创新能力的不断提高,医用水凝胶可以创新性地结合中医药其他传统治疗方法比如针灸、推拿和拔罐等方式进行多角度运用。 展开更多
关键词 医用水凝胶 中医药 研究策略 药物载体 组织工程 骨髓间充质干细胞 联合应用 原位注射
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全反式视黄酸调控颌骨骨髓间充质干细胞成骨分化双向效应的体外研究
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作者 刘媛琪 孙思远 +2 位作者 代庆刚 江凌勇 沈国芳 《上海交通大学学报(医学版)》 CAS CSCD 北大核心 2024年第9期1083-1093,共11页
目的·探究不同浓度全反式视黄酸(all-trans retinoic acid,ATRA)对大鼠颌骨骨髓间充质干细胞(jaw bone marrow mesenchymal stem cells,jBMSCs)成骨分化的影响。方法·通过全骨髓贴壁法分离培养4周龄Sprague-Dawley(SD)大鼠jBM... 目的·探究不同浓度全反式视黄酸(all-trans retinoic acid,ATRA)对大鼠颌骨骨髓间充质干细胞(jaw bone marrow mesenchymal stem cells,jBMSCs)成骨分化的影响。方法·通过全骨髓贴壁法分离培养4周龄Sprague-Dawley(SD)大鼠jBMSCs。运用流式细胞术鉴定jBMSCs表面抗原。利用碱性磷酸酶(alkaline phosphatase,ALP)染色/茜素红染色、油红O染色及阿尔辛蓝染色分别对成骨诱导、成脂诱导及成软骨诱导后的jBMSCs进行多向分化潜能检测。分别使用ATRA浓度为0.01、0.1、1、5、10、20μmol/L的成骨诱导液对jBMSCs进行体外成骨诱导,并使用二甲基亚砜(dimethyl sulfoxide,DMSO)作为对照组,利用CCK8进行细胞活力检测。采用ALP染色和茜素红染色对各浓度组jBMSCs的成骨分化能力进行检测,并筛选后续实验浓度。利用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qPCR)、免疫荧光染色分析不同浓度ATRA下jBMSCs成骨相关基因mRNA与蛋白表达水平。结果·流式细胞术分析显示,98%以上的P1代jBMSCs表现为CD29+CD90+CD31−CD45−,与骨髓间充质干细胞表面抗原特点相符。ALP染色/茜素红染色、油红O染色及阿尔辛蓝染色结果证明P1代jBMSCs具有成骨、成脂、成软骨多向分化能力。ALP染色/茜素红染色结果显示,0.01、0.1和1μmol/L ATRA组jBMSCs成骨活性和矿化能力较对照组增强,而继续提升ATRA浓度则使成骨活性与矿化能力减弱,浓度高于5μmol/L时开始低于对照组水平(均P<0.05)。qPCR分析发现,0.1、1μmol/L ATRA组成骨相关基因如Alp、骨唾液酸蛋白(bone sialoprotein,Bsp)、Ⅰ型胶原蛋白α1(collagen typeⅠα1,Col1a1)、骨钙素(osteocalcin,Ocn)表达水平较对照组上升,而继续提升ATRA浓度则使基因表达水平下降,ATRA浓度高于5μmol/L时开始低于对照组水平(均P<0.05)。免疫荧光染色结果显示,0.1、1μmol/L ATRA组较对照组成骨相关蛋白成骨细胞特异性转录因子SP7、ALP及OCN表达增强,而继续提升ATRA浓度则使蛋白表达下降,浓度高于5μmol/L时开始低于对照组水平(均P<0.05)。结论·较低浓度(0.1、1μmol/L)ATRA可促进大鼠jBMSCs成骨分化能力,且该促进效应在0.1μmol/L浓度时达到峰值,进一步提升浓度可使该促进效应减弱。较高浓度(5、10、20μmol/L)ATRA对大鼠jBMSCs成骨分化呈现抑制效应。研究在体外证明ATRA对大鼠jBMSCs成骨分化具双向效应,并鉴定了0.1μmol/L ATRA为大鼠jBMSCs成骨分化的最适浓度,为体内研究的开展与全反式视黄酸的临床应用提供了参考依据。 展开更多
关键词 全反式视黄酸 颌骨 骨髓间充质干细胞 成骨分化 双向效应
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Effect of HuoguⅡFormula(活骨Ⅱ方) with Medicinal Guide Radix Achyranthis Bidentatae on Bone Marrow Stem Cells Directional Homing to Necrosis Area after Osteonecrosis of the Femoral Head in Rabbit 被引量:11
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作者 孔祥英 王荣田 +3 位作者 田能 李莉 林娜 陈卫衡 《Chinese Journal of Integrative Medicine》 SCIE CAS 2012年第10期761-768,共8页
Objective: To investigate the effect of Huogu II Formula (活骨 II方 ) with medicinal guide Radix Achyranthis Bidentatae (Ach) on bone marrow stem cells (BMSCs) homing to necrosis area after osteonecrosis of the... Objective: To investigate the effect of Huogu II Formula (活骨 II方 ) with medicinal guide Radix Achyranthis Bidentatae (Ach) on bone marrow stem cells (BMSCs) homing to necrosis area after osteonecrosis of the femoral head (ONFH) frozen by liquid nitrogen in rabbit as well as to explore the mechanism of prevention and treatment for ONFH. Methods: The animal model of ONFH was established by liquid nitrogen frozen on the rabbit left hind leg. Forty-eight Japanese White rabbits were randomly assigned to sham-operated group, model group, Huogu II group, and Huogu II plus Ach group, with 12 rabbits in each. During the course of ONFH animal model establishment, all rabbits were subcutaneously injected with recombinant human granulocyte colony-stimulating factor [rhG-CSF, 30 μg/(kg.day) for continuous 7 days]. Meanwhile, normal saline and decoction of the two formulae were administrated by gavage, respectively. White blood cells (WBC) were counted in peripheral blood before and after injection of rhG-CSF. Materials were drawn on the 2rid and 4th weeks after model built; bone glutamine protein (BGP) and bone morphogenetic protein 2 (BMP2) levels in serum were tested. Histopathologic changes were observed by hematoxylin and eosin (HE) staining. BMP2 mRNA levels were detected with in situ hybridization (iSH) staining. 5-Bromo-2'-deoxyuridine (BrdU) and stromal cell derived factor 1 (SDF-1) were measured by immunohistochemical assay in femoral head of the left hind leg. Results: Compared with the shamoperated group, the ratio of empty lacuna, serum BGP, and SDF-1 level in the model group increased significantly, and BMP2 in both serum and femoral head decreased significantly. However, in comparison with the model group, the empty lacuna ratio of Huogu II group and Huogu II plus Ach group decreased obviously in addition to the levels of serum BGP and BMP2, and the expressions of BMP2 mRNA, BrdU, and SDF-1 increased significantly. Above changes were particularly obvious in Huogu II plus Ach group. BGP and SDF-1 on the 2nd week and empty lacuna rate and serum BMP2 level on the 4th week in Huogu II group significantly exceeded their counterparts. On the 2nd week, only in Huogu II plus Ach group that the BrdU counting rose significantly. On the 4th week, empty lacuna rate and serum BMP2 level in Huogu II plus Ach group exceeded those in Huogu II group distinctively. Conclusions: To a certain extent, the medicinal guide Ach improves the preventive and therapeutic effects of Huogu II Formula on expedmental ONFH model. The possible mechanism of this is related to its promoting effect on directional homing of BMSCs to the necrosis area. 展开更多
关键词 osteonecrosis of the femoral head bone marrow stem cells HOMING guide drug to affected area
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他汀类药物的骨靶向递送:联合用药策略与剂量效应关系
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作者 张豫吉 马明 +1 位作者 谢代俊 耿彬 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第10期1524-1528,共5页
传统研究表明,他汀类药物通过影响成骨细胞和破骨细胞的活性对骨代谢产生积极影响。然而,受不良反应和药物疗效的限制,结合纳米技术的骨靶向给药已成为一个新的研究方向。笔者总结了近年来他汀类药物纳米颗粒给药系统的研究情况;纳米技... 传统研究表明,他汀类药物通过影响成骨细胞和破骨细胞的活性对骨代谢产生积极影响。然而,受不良反应和药物疗效的限制,结合纳米技术的骨靶向给药已成为一个新的研究方向。笔者总结了近年来他汀类药物纳米颗粒给药系统的研究情况;纳米技术为未来减少不良反应和提高疗效提供了新的可能性。这些系统利用其精确的药物释放能力有效靶向骨组织,优化疗效并减少全身不良反应。此外,他汀类药物在治疗骨质疏松症时剂量不同的研究也层出不穷。今后,在开发骨靶向给药和纳米技术的组合时,需要考虑他汀类药物的剂量效应关系。总之,他汀类药物在骨质疏松症治疗中的应用是一个多方面的领域,需要综合药物作用机制、剂量效应、纳米技术应用及其临床转化潜力等方面进行深入研究。未来的研究应侧重于探索这些药物的最佳应用,以确保其在调节骨代谢方面的安全性和有效性。 展开更多
关键词 他汀类药物 骨质疏松 骨靶向药物递送系统 剂量-效应
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