Polyetheretherketone （PEEK） is regarded as one of the most potential candidates of biomaterials in spinal implant applications. However, as a bioinert material, PEEK plays a limited role in osteoconduction and osseo...Polyetheretherketone （PEEK） is regarded as one of the most potential candidates of biomaterials in spinal implant applications. However, as a bioinert material, PEEK plays a limited role in osteoconduction and osseointegration. In this study, recombinant human bone morphogenetic protein-2 （rhBMP-2） was immobilized onto the surface of collagen-coated PEEK in order to prepare a multi-functional material. After adsorbed onto the PEEK surface by hydroph（bic interaction, collagen was cross-linked with N-（3-dimethylaminopropyl）-N＇-ethyl carbodiimide hydrochloride （EDC） and N-hydro xysuccinimide （NHS）. EDCINHS system also contributed to the immobilization of rhBMP- 2. Water contact angle tests, XPS and SEM clearly demonstrated the surface changes. ELISA tests quantified the amount of rhBMP-2 immobilized and the release over a period of 30 d. In vitro evaluation proved that the osteogenesis differentiation rate was higher when cells were cultured on modified PEEK discs than on regular ones. In vivo tests were conducted and positive changes of major parameters were presented. This report demonstrates that the rhBMP-2 immobilized method for PEEK modification increase bioactivity in vitro and in vivo, suggesting its practicability in orthopedic and spinal clinical applications.展开更多
基金Acknowledgements This work was supported by the International Scientific and Technological Cooperation Projects of Shenzhen Science and Technology R ＆ D Foundation （ZYA201106090054A） and the National Natural Science Foundation of China （Grant No. 31200727, H. M. Nie）.
文摘Polyetheretherketone （PEEK） is regarded as one of the most potential candidates of biomaterials in spinal implant applications. However, as a bioinert material, PEEK plays a limited role in osteoconduction and osseointegration. In this study, recombinant human bone morphogenetic protein-2 （rhBMP-2） was immobilized onto the surface of collagen-coated PEEK in order to prepare a multi-functional material. After adsorbed onto the PEEK surface by hydroph（bic interaction, collagen was cross-linked with N-（3-dimethylaminopropyl）-N＇-ethyl carbodiimide hydrochloride （EDC） and N-hydro xysuccinimide （NHS）. EDCINHS system also contributed to the immobilization of rhBMP- 2. Water contact angle tests, XPS and SEM clearly demonstrated the surface changes. ELISA tests quantified the amount of rhBMP-2 immobilized and the release over a period of 30 d. In vitro evaluation proved that the osteogenesis differentiation rate was higher when cells were cultured on modified PEEK discs than on regular ones. In vivo tests were conducted and positive changes of major parameters were presented. This report demonstrates that the rhBMP-2 immobilized method for PEEK modification increase bioactivity in vitro and in vivo, suggesting its practicability in orthopedic and spinal clinical applications.
