[Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.[Method]The d...[Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.[Method]The distribution characteristics of Cu2+ in subcells of Setcreasea purpurea Boom was studied by the technique of differential centrifugation,and the binding form of Cu2+ in roots and leaves of Setcreasea purpurea Boom was also investigated by the sequential chemical extraction method and the enzymolysis method.[Result]Cu2+ in roots mainly distributed in the cell wall which is accounting for one third of the Total Cu2+ in roots,while Cu2+ in leaves mainly distributed in the chloroplast which is accounting for a quarter to the Total Cu2+ in leaves.Under the high concentration of Cu2+ or the extended treatment duration,the translocation of Cu2+ in root cells into the cell wall increased but the translocation of Cu2+ in root cells into the plastid decreased,while the translocation of Cu2+ in leaf cells into the chloroplast increased but the translocation of Cu2+ in leaf cells into the cell wall decreased.Cu2+ in leaves was mainly combined with amino acid,small molecular polymeric pigments,protein and polysaccharide,while Cu2+ in roots was mainly combined with cell wall substances such as cellulose and membrane-bound protein.[Conclusion]The distribution characteristics and binding form of Cu2+ in cells is possibly one of the dominant mechanisms for Cu2+ hyper-accumulator in Setcreasea purpurea Boom.展开更多
[Objective] This study was to investigate the expression of the specific protein in Setcreasea purpurea Boom under copper stress, with the aim to clarify the copper tolerance mechanism of S. purpurea. [Method] Methods...[Objective] This study was to investigate the expression of the specific protein in Setcreasea purpurea Boom under copper stress, with the aim to clarify the copper tolerance mechanism of S. purpurea. [Method] Methods of water culture, elec- trophoresis and chromatography were used to analyze the molecular weight of the specific protein in the copper hyperaccumulator S. purpurea, as well as its expression time and the minimum copper concentration for the expression. And the specific protein was isolated and purified. [Result] Under copper stress, the minimum concentra- tion of copper to induce the expression of the specific protein from S. purpurea was 50 umol/L, and the expression time of the protein was in the 4th week with the molecular weight of 89.4 kDa. [Conclusion] The results show that the copper tolerance of S. purpurea is closely related with the expression of the specific protein.展开更多
[Objective] The aim was to explore biological function of related genes in Setcreasea purpurea Boom under copper stress and to study the mechanism of its copper-resistance from standpoint of molecular biology in order...[Objective] The aim was to explore biological function of related genes in Setcreasea purpurea Boom under copper stress and to study the mechanism of its copper-resistance from standpoint of molecular biology in order to solve the problem of copper pollution. [Method] Setcreasea purpurea Boom was taken as experimental material which enjoys enrichment ability to cupric ion. About 90 fragments of differential expression were obtained by cDNA-AFLP and silver staining technique, among which, 17 fragments were amplified. After purification and identification, sequences of 6 differential fragments were got and used for BLAST X contrast. [Result] Six differential expressed fragments may play roles when Setcreasea purpurea Boom was under copper stress. The homology achieved 49% between differential sequences of E5MG-3 and of Arabidopsis thaliana mRNA (accession numbers: AAM62956.1), homology was 53% between sequences of E4MB-2 and Solanum tuberosum mRNA (accession numbers: A5A717.1), and 65% between sequences of E6MG-1 and Colocasia esculenta (L.) Schott mRNA (accession numbers: AAO62313.1). It can be concluded that differential expressed genes are related to cell signaling, antioxidation, metabolism and protein modification. [Conclusion] The study has laid foundation for further exploration of regulatory network about response of Setcreasea purpurea Boom to copper stress.展开更多
BOOMBOX译码器在黄土塬地区施工中,采用多井组合激发时,由于激发网络电阻增大,致使拒爆、丢炮和丢失验证时断信号(confirmation time break,CTB)的现象频发,严重制约了地震生产。针对该译码器的激发能力问题,分别从点火电压、...BOOMBOX译码器在黄土塬地区施工中,采用多井组合激发时,由于激发网络电阻增大,致使拒爆、丢炮和丢失验证时断信号(confirmation time break,CTB)的现象频发,严重制约了地震生产。针对该译码器的激发能力问题,分别从点火电压、充电时间、点火电流和CTB产生信号原理等方面进行了详尽的分析研究,并通过电路改进和现场试验,找到了提高其激发能力的一种较为便捷的方法。通过现场使用,取得了较好效果。展开更多
Boom Clay is one of the potential host rocks for deep geological disposal of high-level radioactive nuclear waste in Belgium. In order to investigate the mechanism of hydraulic conductivity variation under complex the...Boom Clay is one of the potential host rocks for deep geological disposal of high-level radioactive nuclear waste in Belgium. In order to investigate the mechanism of hydraulic conductivity variation under complex thermo-mechanical coupling conditions and to better understand the thermo-hydromechanical(THM) coupling behaviour of Boom Clay, a series of permeability tests using temperaturecontrolled triaxial cell has been carried out on the Boom Clay samples taken from Belgian underground research laboratory(URL) HADES. Due to its sedimentary nature, Boom Clay presents acrossanisotropy with respect to its sub-horizontal bedding plane. Direct measurements of the vertical(Kv)and horizontal(Kh)hydraulic conductivities show that the hydraulic conductivity at 80℃ is about 2.4 times larger than that at room temperature(23℃), and the hydraulic conductivity variation with temperature is basically reversible during heatingecooling cycle. The anisotropic property of Boom Clay is studied by scanning electron microscope(SEM) tests, which highlight the transversely isotropic characteristics of intact Boom Clay. It is shown that the sub-horizontal bedding feature accounts for the horizontal permeability higher than the vertical one. The measured increment in hydraulic conductivity with temperature is lower than the calculated one when merely considering the changes in water kinematic viscosity and density with temperature. The nuclear magnetic resonance(NMR) tests have also been carried out to investigate the impact of microstructure variation on the THM properties of clay. The results show that heating under unconstrained boundary condition will produce larger size of pores and weaken the microstructure. The discrepancy between the hydraulic conductivity experimentally measured and predicted(considering water viscosity and density changes with temperature) can be attributed to the microstructural weakening effect on the thermal volume change behaviour of Boom Clay. Based on the experimental results, a hydraulic conductivity evolution model is proposed and then implemented in ABAQUS. Three-dimensional(3D) numerical simulation of the admissible thermal loading for argillaceous storage(ATLAS) Ⅲ in situ heating test has been conducted subsequently, and the numerical results are in good agreement with field measurements.展开更多
Due to the insufficiency of utilizing knowledge to guide the complex optimal searching, existing genetic algorithms fail to effectively solve excavator boom structural optimization problem. To improve the optimization...Due to the insufficiency of utilizing knowledge to guide the complex optimal searching, existing genetic algorithms fail to effectively solve excavator boom structural optimization problem. To improve the optimization efficiency and quality, a new knowledge-based real-coded genetic algorithm is proposed. A dual evolution mechanism combining knowledge evolution with genetic algorithm is established to extract, handle and utilize the shallow and deep implicit constraint knowledge to guide the optimal searching of genetic algorithm circularly. Based on this dual evolution mechanism, knowledge evolution and population evolution can be connected by knowledge influence operators to improve the conflgurability of knowledge and genetic operators. Then, the new knowledge-based selection operator, crossover operator and mutation operator are proposed to integrate the optimal process knowledge and domain culture to guide the excavator boom structural optimization. Eight kinds of testing algorithms, which include different genetic operators, arc taken as examples to solve the structural optimization of a medium-sized excavator boom. By comparing the results of optimization, it is shown that the algorithm including all the new knowledge-based genetic operators can more remarkably improve the evolutionary rate and searching ability than other testing algorithms, which demonstrates the effectiveness of knowledge for guiding optimal searching. The proposed knowledge-based genetic algorithm by combining multi-level knowledge evolution with numerical optimization provides a new effective method for solving the complex engineering optimization problem.展开更多
In this investigation, a modeling procedure of a telescopic boom of cranes is developed using the absolute nodal coordinate formulation together with the sliding joint constraints. Since telescopic booms are extracted...In this investigation, a modeling procedure of a telescopic boom of cranes is developed using the absolute nodal coordinate formulation together with the sliding joint constraints. Since telescopic booms are extracted and retracted under various operating conditions, the overall length of the boom changes dynamically, leading to the time-variant vibration characteristics. For modeling the telescopic structure of booms, a special care needs to be exercised since the location of the sliding contact point moves Mong the deformable axis of the flexible boom and the solution to a moving boundary problem is required. This issue indeed makes the modeling of the telescopic boom difficult, despite the significant needs for the analysis. It is, therefore, the objective of this investigation to develop a modeling procedure for the flexible telescopic boom by considering the sliding contact condition with the dynamic frictional effect. To this end, the sliding joint constraint developed for the absolute nodal coordinate formulation is employed for describing relative sliding motion between flexible booms, while flexible booms are modeled using the beam element of the absolute nodal coordinate formulation, which allows for modeling the large rotation and deformation of the structure.展开更多
基金Supported by the National Natural Science Foundation(30760021)Natural Science Foundation of Jiangxi Province(0530016)Project of the Education Department of Jiangxi Province(Jiangxi Education&Tech-nology No.[2007]151)~~
文摘[Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.[Method]The distribution characteristics of Cu2+ in subcells of Setcreasea purpurea Boom was studied by the technique of differential centrifugation,and the binding form of Cu2+ in roots and leaves of Setcreasea purpurea Boom was also investigated by the sequential chemical extraction method and the enzymolysis method.[Result]Cu2+ in roots mainly distributed in the cell wall which is accounting for one third of the Total Cu2+ in roots,while Cu2+ in leaves mainly distributed in the chloroplast which is accounting for a quarter to the Total Cu2+ in leaves.Under the high concentration of Cu2+ or the extended treatment duration,the translocation of Cu2+ in root cells into the cell wall increased but the translocation of Cu2+ in root cells into the plastid decreased,while the translocation of Cu2+ in leaf cells into the chloroplast increased but the translocation of Cu2+ in leaf cells into the cell wall decreased.Cu2+ in leaves was mainly combined with amino acid,small molecular polymeric pigments,protein and polysaccharide,while Cu2+ in roots was mainly combined with cell wall substances such as cellulose and membrane-bound protein.[Conclusion]The distribution characteristics and binding form of Cu2+ in cells is possibly one of the dominant mechanisms for Cu2+ hyper-accumulator in Setcreasea purpurea Boom.
基金Supported by the National Natural Science Foundation of China (30760021)the Natural Science Foundation of Jiangxi Province,China (0530016)the Program of Education Bureau of Jiangxi Province,China (ganjiaojizi[2007]No.151)~~
文摘[Objective] This study was to investigate the expression of the specific protein in Setcreasea purpurea Boom under copper stress, with the aim to clarify the copper tolerance mechanism of S. purpurea. [Method] Methods of water culture, elec- trophoresis and chromatography were used to analyze the molecular weight of the specific protein in the copper hyperaccumulator S. purpurea, as well as its expression time and the minimum copper concentration for the expression. And the specific protein was isolated and purified. [Result] Under copper stress, the minimum concentra- tion of copper to induce the expression of the specific protein from S. purpurea was 50 umol/L, and the expression time of the protein was in the 4th week with the molecular weight of 89.4 kDa. [Conclusion] The results show that the copper tolerance of S. purpurea is closely related with the expression of the specific protein.
基金Supported by National Natural Science Foundation of China(30760021)Natural Science Foundation of Jiangxi Province(0530016)Project from Education Bureau of Jiangxi Province([2007]151)~~
文摘[Objective] The aim was to explore biological function of related genes in Setcreasea purpurea Boom under copper stress and to study the mechanism of its copper-resistance from standpoint of molecular biology in order to solve the problem of copper pollution. [Method] Setcreasea purpurea Boom was taken as experimental material which enjoys enrichment ability to cupric ion. About 90 fragments of differential expression were obtained by cDNA-AFLP and silver staining technique, among which, 17 fragments were amplified. After purification and identification, sequences of 6 differential fragments were got and used for BLAST X contrast. [Result] Six differential expressed fragments may play roles when Setcreasea purpurea Boom was under copper stress. The homology achieved 49% between differential sequences of E5MG-3 and of Arabidopsis thaliana mRNA (accession numbers: AAM62956.1), homology was 53% between sequences of E4MB-2 and Solanum tuberosum mRNA (accession numbers: A5A717.1), and 65% between sequences of E6MG-1 and Colocasia esculenta (L.) Schott mRNA (accession numbers: AAO62313.1). It can be concluded that differential expressed genes are related to cell signaling, antioxidation, metabolism and protein modification. [Conclusion] The study has laid foundation for further exploration of regulatory network about response of Setcreasea purpurea Boom to copper stress.
文摘BOOMBOX译码器在黄土塬地区施工中,采用多井组合激发时,由于激发网络电阻增大,致使拒爆、丢炮和丢失验证时断信号(confirmation time break,CTB)的现象频发,严重制约了地震生产。针对该译码器的激发能力问题,分别从点火电压、充电时间、点火电流和CTB产生信号原理等方面进行了详尽的分析研究,并通过电路改进和现场试验,找到了提高其激发能力的一种较为便捷的方法。通过现场使用,取得了较好效果。
基金financial support of the National Science Foundation for Distinguished Young Scholars (Grant No. 51225902)Natural Science Foundation of China (Grant No. 51479190)EURIDICE (European Underground Research Infrastructure for Disposal of Nuclear Waste in Clay Environment, Mol, Belgium) for the work presented in this paper
文摘Boom Clay is one of the potential host rocks for deep geological disposal of high-level radioactive nuclear waste in Belgium. In order to investigate the mechanism of hydraulic conductivity variation under complex thermo-mechanical coupling conditions and to better understand the thermo-hydromechanical(THM) coupling behaviour of Boom Clay, a series of permeability tests using temperaturecontrolled triaxial cell has been carried out on the Boom Clay samples taken from Belgian underground research laboratory(URL) HADES. Due to its sedimentary nature, Boom Clay presents acrossanisotropy with respect to its sub-horizontal bedding plane. Direct measurements of the vertical(Kv)and horizontal(Kh)hydraulic conductivities show that the hydraulic conductivity at 80℃ is about 2.4 times larger than that at room temperature(23℃), and the hydraulic conductivity variation with temperature is basically reversible during heatingecooling cycle. The anisotropic property of Boom Clay is studied by scanning electron microscope(SEM) tests, which highlight the transversely isotropic characteristics of intact Boom Clay. It is shown that the sub-horizontal bedding feature accounts for the horizontal permeability higher than the vertical one. The measured increment in hydraulic conductivity with temperature is lower than the calculated one when merely considering the changes in water kinematic viscosity and density with temperature. The nuclear magnetic resonance(NMR) tests have also been carried out to investigate the impact of microstructure variation on the THM properties of clay. The results show that heating under unconstrained boundary condition will produce larger size of pores and weaken the microstructure. The discrepancy between the hydraulic conductivity experimentally measured and predicted(considering water viscosity and density changes with temperature) can be attributed to the microstructural weakening effect on the thermal volume change behaviour of Boom Clay. Based on the experimental results, a hydraulic conductivity evolution model is proposed and then implemented in ABAQUS. Three-dimensional(3D) numerical simulation of the admissible thermal loading for argillaceous storage(ATLAS) Ⅲ in situ heating test has been conducted subsequently, and the numerical results are in good agreement with field measurements.
基金supported by National Natural Science Foundation of China(Grant No.51175086)
文摘Due to the insufficiency of utilizing knowledge to guide the complex optimal searching, existing genetic algorithms fail to effectively solve excavator boom structural optimization problem. To improve the optimization efficiency and quality, a new knowledge-based real-coded genetic algorithm is proposed. A dual evolution mechanism combining knowledge evolution with genetic algorithm is established to extract, handle and utilize the shallow and deep implicit constraint knowledge to guide the optimal searching of genetic algorithm circularly. Based on this dual evolution mechanism, knowledge evolution and population evolution can be connected by knowledge influence operators to improve the conflgurability of knowledge and genetic operators. Then, the new knowledge-based selection operator, crossover operator and mutation operator are proposed to integrate the optimal process knowledge and domain culture to guide the excavator boom structural optimization. Eight kinds of testing algorithms, which include different genetic operators, arc taken as examples to solve the structural optimization of a medium-sized excavator boom. By comparing the results of optimization, it is shown that the algorithm including all the new knowledge-based genetic operators can more remarkably improve the evolutionary rate and searching ability than other testing algorithms, which demonstrates the effectiveness of knowledge for guiding optimal searching. The proposed knowledge-based genetic algorithm by combining multi-level knowledge evolution with numerical optimization provides a new effective method for solving the complex engineering optimization problem.
基金supported by Kato Construction Machine Research & Development Promotion Fund
文摘In this investigation, a modeling procedure of a telescopic boom of cranes is developed using the absolute nodal coordinate formulation together with the sliding joint constraints. Since telescopic booms are extracted and retracted under various operating conditions, the overall length of the boom changes dynamically, leading to the time-variant vibration characteristics. For modeling the telescopic structure of booms, a special care needs to be exercised since the location of the sliding contact point moves Mong the deformable axis of the flexible boom and the solution to a moving boundary problem is required. This issue indeed makes the modeling of the telescopic boom difficult, despite the significant needs for the analysis. It is, therefore, the objective of this investigation to develop a modeling procedure for the flexible telescopic boom by considering the sliding contact condition with the dynamic frictional effect. To this end, the sliding joint constraint developed for the absolute nodal coordinate formulation is employed for describing relative sliding motion between flexible booms, while flexible booms are modeled using the beam element of the absolute nodal coordinate formulation, which allows for modeling the large rotation and deformation of the structure.