Chemically robust conductive p-type boron-doped diamond(BDD) films are an important electrode material and have been widely applied in electrochemistry.In this study,BDD films are taken as a two-dimensional(2D)electro...Chemically robust conductive p-type boron-doped diamond(BDD) films are an important electrode material and have been widely applied in electrochemistry.In this study,BDD films are taken as a two-dimensional(2D)electrode in a electrophoresis tank system instead of the conventional one-dimensional platinum wire electrode.The theoretical simulations by finite element numerical analysis reveal that the 2D BDD electrodes have relatively high intensity and uniformity of electric field in the tank.Experimentally,the 2D BDD electrodes with smaller size show excellent properties for the separation of DNA fragments.The advantages of the 2D BDD electrodes with chemical inertness,sustainability,high intensity and uniformity electronic field,as well as reduced small size of electrophoresis tank would open a possibility for realizing new generation,high-performance biological devices.展开更多
An electroanalytical method was developed for the direct quantitative determination of paracetamol in tablets based on its oxidation behavior. The electrochemical oxidation and determination of paracetamol were easily...An electroanalytical method was developed for the direct quantitative determination of paracetamol in tablets based on its oxidation behavior. The electrochemical oxidation and determination of paracetamol were easily carried out on born-doped diamond (BDD) electrode using two voltammetric techniques (CV and DPV). The electrochemical measurements performed by cyclic voltammetric (CV) and differential pulse voltammetry (DPV) techniques were carried out using a cathodically pretreated boron-doped diamond electrode in HClO4 and KClO4 electrolytes. HClO4 was then selected for analytical purposes and scan rate studies were also completed. The oxidation of the paracetamol is found to be irreversible and a diffusion-controlled nature of the paracetamol oxidation peak was established. A linear calibration curve for DPV analysis was constructed in the paracetamol concentration range from 0 μM to 13.87 μM, with 0.16 μM and 0.55 μM as the detection and quantification limit respectively.展开更多
The direct detection of clenbuterol(CL) in pig liver without any extraction separation at a pyrrole-DNA modified boron-doped diamond(BDD) electrode is reported. The pyrrole-DNA modified BDD electrode has a strong elec...The direct detection of clenbuterol(CL) in pig liver without any extraction separation at a pyrrole-DNA modified boron-doped diamond(BDD) electrode is reported. The pyrrole-DNA modified BDD electrode has a strong electrocatalytic effect on the redox reaction of CL. One oxidization and two reduction peaks of CL appear at 340.2, 299.8 and 166.6 mV(versus SCE), respectively. The pyrrole polymer alone cannot electrocatalyze the above reaction at a BDD electrode; the electrocatalytic effect of a BDD electrode modified with DNA membrane is unsufficient for the analytical detection of CL; the replacement of boron-doped diamond by glass carbon makes the electrocatalytic reaction impossible; the redox process is pH dependent. The influences of various experimental parameters on the pyrrole-DNA modified BDD electrode were investigated. A sensitive cyclic voltammetric response for CL was obtained in a linear range from 3.4×10^-6 to 5×10^-4 mol/L with a detection limit of 8.5×10^-7 mol/L. A mean recovery of 102.7% of CL in the pig liver sample solution and a reproducibility of 3.2% were obtained.展开更多
In this study, a new technique was developed using rapid ultra-performance liquid chromatography (UPLC)-based separation coupled with electrochemical detection by a boron-doped diamond (BDD) electrode for the detectio...In this study, a new technique was developed using rapid ultra-performance liquid chromatography (UPLC)-based separation coupled with electrochemical detection by a boron-doped diamond (BDD) electrode for the detection and quantification of three commonly used parabens (methylparaben (MP), ethylparaben (EP) and propylparaben (PP)). We aimed to reduce the analysis time by using UPLC coupled with a short reverse phase C 18 monolithic column (25 mm×4.6 mm). Operating the monolithic column at low back-pressure resulted in high flow rates. A mobile phaseconsisting of a 25:75 (v/v) ratio of acetonitrile:0.05 Mphosphate buffer (pH 5) at a flow rate of 2.5 mL·min?1 was used to perform the separation. The amperometric detection with the BDD electrode was found to be optimal and reliably reproducible at a detection potential of 1.5 V vs. Ag/AgCl. Under these conditions, the separation of the three targetanalytes (MP, EP and PP) was achieved in 2 min and was linear within a sample concentration range of 0.1 to 50.0 mg·L?1 (r2 values of 0.9970, 0.9994 and 0.9994 for MP, EP and PP, respectively). This method was successfully applied to determine the concentrations of each parabeninsix real samples with therecoveries ranging from of 80.3% - 98.9% for all three parabensfrom samples spiked at 12, 22 and 32 mg·L?1. Therefore, the proposed method can be used as an alternative rapid and selective method for the determination of paraben levels in real samples.展开更多
基金Supported by the National Natural Science Foundation of China under Grant No 51472105the Key Program in Science and Technology of Jilin Province under Grant No 20150204062GX
文摘Chemically robust conductive p-type boron-doped diamond(BDD) films are an important electrode material and have been widely applied in electrochemistry.In this study,BDD films are taken as a two-dimensional(2D)electrode in a electrophoresis tank system instead of the conventional one-dimensional platinum wire electrode.The theoretical simulations by finite element numerical analysis reveal that the 2D BDD electrodes have relatively high intensity and uniformity of electric field in the tank.Experimentally,the 2D BDD electrodes with smaller size show excellent properties for the separation of DNA fragments.The advantages of the 2D BDD electrodes with chemical inertness,sustainability,high intensity and uniformity electronic field,as well as reduced small size of electrophoresis tank would open a possibility for realizing new generation,high-performance biological devices.
文摘An electroanalytical method was developed for the direct quantitative determination of paracetamol in tablets based on its oxidation behavior. The electrochemical oxidation and determination of paracetamol were easily carried out on born-doped diamond (BDD) electrode using two voltammetric techniques (CV and DPV). The electrochemical measurements performed by cyclic voltammetric (CV) and differential pulse voltammetry (DPV) techniques were carried out using a cathodically pretreated boron-doped diamond electrode in HClO4 and KClO4 electrolytes. HClO4 was then selected for analytical purposes and scan rate studies were also completed. The oxidation of the paracetamol is found to be irreversible and a diffusion-controlled nature of the paracetamol oxidation peak was established. A linear calibration curve for DPV analysis was constructed in the paracetamol concentration range from 0 μM to 13.87 μM, with 0.16 μM and 0.55 μM as the detection and quantification limit respectively.
基金Supported by the National Natural Science Foundation of China(Nos. 20435010, 20375012, 20205005 and 20475014).
文摘The direct detection of clenbuterol(CL) in pig liver without any extraction separation at a pyrrole-DNA modified boron-doped diamond(BDD) electrode is reported. The pyrrole-DNA modified BDD electrode has a strong electrocatalytic effect on the redox reaction of CL. One oxidization and two reduction peaks of CL appear at 340.2, 299.8 and 166.6 mV(versus SCE), respectively. The pyrrole polymer alone cannot electrocatalyze the above reaction at a BDD electrode; the electrocatalytic effect of a BDD electrode modified with DNA membrane is unsufficient for the analytical detection of CL; the replacement of boron-doped diamond by glass carbon makes the electrocatalytic reaction impossible; the redox process is pH dependent. The influences of various experimental parameters on the pyrrole-DNA modified BDD electrode were investigated. A sensitive cyclic voltammetric response for CL was obtained in a linear range from 3.4×10^-6 to 5×10^-4 mol/L with a detection limit of 8.5×10^-7 mol/L. A mean recovery of 102.7% of CL in the pig liver sample solution and a reproducibility of 3.2% were obtained.
文摘In this study, a new technique was developed using rapid ultra-performance liquid chromatography (UPLC)-based separation coupled with electrochemical detection by a boron-doped diamond (BDD) electrode for the detection and quantification of three commonly used parabens (methylparaben (MP), ethylparaben (EP) and propylparaben (PP)). We aimed to reduce the analysis time by using UPLC coupled with a short reverse phase C 18 monolithic column (25 mm×4.6 mm). Operating the monolithic column at low back-pressure resulted in high flow rates. A mobile phaseconsisting of a 25:75 (v/v) ratio of acetonitrile:0.05 Mphosphate buffer (pH 5) at a flow rate of 2.5 mL·min?1 was used to perform the separation. The amperometric detection with the BDD electrode was found to be optimal and reliably reproducible at a detection potential of 1.5 V vs. Ag/AgCl. Under these conditions, the separation of the three targetanalytes (MP, EP and PP) was achieved in 2 min and was linear within a sample concentration range of 0.1 to 50.0 mg·L?1 (r2 values of 0.9970, 0.9994 and 0.9994 for MP, EP and PP, respectively). This method was successfully applied to determine the concentrations of each parabeninsix real samples with therecoveries ranging from of 80.3% - 98.9% for all three parabensfrom samples spiked at 12, 22 and 32 mg·L?1. Therefore, the proposed method can be used as an alternative rapid and selective method for the determination of paraben levels in real samples.
