将boronic acid定名为焩酸的建议

建议沿用类似于中文化学术语中已有的磷—膦、碳—羧、硫—磺等的转化规则,将含C—B键的"烃基硼酸"及其衍生物定名为"焩酸"及其衍生物。"焩"音恰为"硼"和"烃"的切合音,其偏旁"火"恰与烃类(烷、烯、炔)的系统偏旁相合。在化学术语中,有许多已被广泛使用的类似"焩"切合字,如"烃"(碳氢切合音)、"羰"(碳氧切合音)、"巯"(氢硫切合音)、"羟"(氢氧切合音)等。

Green Synthesis of Aryl Thioethers through Cu-catalysed C-S Coupling of Thiols and Aryl Boronic Acids in Water

An efficient, practical, highly selective and environmentally benign method is reported for the synthesis of aryl thioethers via the coupling of thiols with aryl boronic acids in the presence of NaOH and a catalytic amount of CuSO4 at 130 ℃ using water as a green solvent. The products were obtained in moderate to excellent yields;more importantly, the use of toxic ligands and solvents was avoided. A broad range of aryl boronic acids and scalable processes make this methodology valuable and versatile for the synthesis of a broad range of aryl sulfides.

Direct Colorimetric Detection of Hydrogen Peroxide Using 4-Nitrophenyl Boronic Acid or Its Pinacol Ester

A colorimetric method for the direct determination of hydrogen peroxide in aqueous solution is described. H2O2 stoichiometrically converts 4-nitrophenyl boronic acid or 4-nitrophenyl boronic acid pinacol ester into 4-nitrophenol, which can be quantified by measuring the absorption at 400 nm in neutral or basic media. The reactions proceed fast under basic conditions and complete in 2 minutes to at pH 11 and 80?C. The linear range for the colorimetric method extends beyond 1.0 to 40 μM H2O2, and the limit of detection is ~1.0 μM H2O2. This method offers a convenient and practical process for rapid determination of hydrogen peroxide in aqueous media. Compared to many other techniques in H2O2 detection, this process is a direct measurement of H2O2, and is relatively unaffected by the presence of various salts, metal ions and the chelator EDTA.

Boronic Acids as Ligands for Affinity Chromatography

A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates,nucleic acid components,glycoproteins,and other small biomolecules.The mechanisms of interactions between boronate ligands and analytes are described.Various boronate ligands and supports are discussed.Examples of the use of boronate affinity chromatography for separation of each class of analytes are presented.

Copper-mediated synthesis of N,N'-diarylhydrazines from boronic acids

N,N＇-Diarylhydrazines have been prepared via the reaction of N-Boc-aryl hydrazines with boronic acids mediated by copper acetate. This mild reaction condition tolerates the presence of a variety of functional groups.

A High Sensitive LC-MS/MS Method for the Simultaneous Determination of Potential Genotoxic Impurities Carboxy Phenyl Boronic Acid and Methyl Phenyl Boronic Acid in Lumacaftor

A simple, rapid, and highly sensitive LC-MS/MS method has been developed for the simultaneous and trace level quantification of underivatized boronic acids in lumacaftor active pharmaceutical ingredient. Chromatographic separation of boronic acids and lumacaftor achieved using Agilent Poroshell HPH C18 150 × 4.6 mm 2.7 μ column with 0.1% ammonia in water as mobile phase A and 100% acetonitrile as mobile phase B at a flow rate of 0.25 ml/min. Gradient elution was used with a total method run time of 14 minutes. Boronic acids were successfully ionized and quantified without derivatization using electrospray ionization in negative mode using tandem quadrupole mass spectrometry in multiple reactions monitoring mode. Method validation was performed as per ICH guidelines with good linearity over the concentration range of 0.05 ppm to 5 ppm of Lumacaftor test concentration for both the boronic acids with a correlation coefficient of >0.99. Recoveries were found good at different concentration levels and within the range of 80% - 120%. The developed method can be successfully used for the routine quantification of boronic acids at a concentration level of 20 ng/ml (1 ppm with respect to 20 mg/ml lumacaftor).

Lactic Acid Reduces LPS-Induced TNF-a and IL-6 mRNA Levels Through Decreasing I?Ba Phosphorylation

This study explored the effects over time of lactic acid （LA） on IκBα phosphorylation and nuclear factor-kappa B （NF-κB） p65 protein expression, and on tumor necrosis factor a （TNF-α） and interleukin-6 （IL-6） mRNA levels in rat intestinal mucosa microvascular endothelial cells （RIMMVECs） stimulated by lipopolysaccharide （LPS）. I？Ba, phosphorylated IκBa （p-IκBa） and p65 protein levels were monitored by Western blot analysis, and TNF-a and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF-a and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on IκBa phosphorylation to prevent activation of NF-κB was after 6 h. These results suggest that LA reduces TNF-a and IL-6 mRNA levels through decreasing IκBa phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF-κB.

Boron Nitride Nanotubes as Novel Vectors for Drug Delivery of Amino Acids：A First Principles Simulation

The first principles calculations based on density functional theory（DFT） were performed for investigating the interaction of amino acids with（5, 5） armchair and（8, 0） zigzag boron nitride nanotubes（BNNTs）. Findings showed that the adsorption and solvation energies were negative for（5, 5）/（8,0） BNNTs-amino acid complexes, implying the thermodynamic favorability and spontaneous interactions of amino acids with BNNTs sidewall. Based on calculated results, the BNNTs are expected to be a potential efficient adsorbent as well as a suitable drug delivery vehicle for the adsorption of amino acids within biological systems.

A 3D Ba(Ⅱ) Inorganic-organic Hybrid Framework Based on 1,3,5-Benzenetricarboxylic Acid Ligand: Synthesis and Characterization

A three-dimensional（3D） barium complex with 1,3,5-benzenetricarboxylic acid（H3BTC）, {[Ba1.5（BTC）（H2O）]·（H2O）}n（1）, was synthesized in DMF/EtOH/H2O mixed solution under solvothermal conditions, and characterized by single-crystal X-ray diffraction, elemental analyses, IR spectra, thermogravimetric analyses, and photoluminescence measurement. In complex 1, the 2D I2O0 type inorganic layer is constructed by {Ba1O10} and {Ba2O9} polyhedra. Moreover, the solid-state fluorescence measurement reveals a fluorescence emission band at 465 nm under 344 nm excitation, assigned to a charge-transfer transition.

Combined DFT and experiment:Stabilizing the electrochemical interfaces via boron Lewis acids

The incorporation of boron into carbon material can significantly enhance its capacity performances.However,the origin of the promotion effect of boron doping on electrochemical performances is still unclear,in part due to the inadequate exposure of boron configurations resulting from the complexity of traditional carbon materials.To overcome this issue,herein,a series of boron-doped graphene with highly-exposed boron configurations are prepared by tuning annealing temperature.Then the correlation between boron configurations and the electrochemical performances is investigated.The combination of density-functional theory(DFT)computation and NH3-TPD/Py-FTIR indicates that the BCO_(2)configuration formed on the surface of graphene is easier to accept lone-pair electrons than BC_(2)O and BC_(3)configurations due to the stronger Lewis acidity.Such an electronic structure can effectively reduce the number of unstable electron donors and stabilize the electrochemical interface,which is proved by NMR,and critical for improving the electrochemical performances.Further experiments confirm that the optimized BG800 with the largest amount of BCO_(2)configuration presents ultralow leak current,improved cyclic stability,and better rate performance in SBPBF4/PC.This work would provide an insight into the design of high-performance boron-doped carbon materials towards energy storage.

RT-PCR/CRISPR-Cas12a快速检测和区分SARS-CoV-2 Omicron BA.4/5变异株

目的建立一种基于CRISPPR-Cas12a基因编辑技术对新型冠状病毒(SARS-CoV-2)奥密克戎BA.4/5变异株快速检测与鉴别的方法。方法结合逆转录聚合酶链式反应(RT-PCR)与CRISPR基因编辑技术,基于次优原间隔区相邻基序(suboptimal-PAM)设计奥密克戎BA.4/5变异株特异性的CRISPRRNA(crRNA),从而建立RT-PCR/CRISPR-Cas12a快速检测新冠病毒BA.4/5变异株的方法。本研究检测了43例新冠病毒阳性的临床样本(包括新冠病毒野生株以及变异株Alpha、Beta、Delta、奥密克戎BA.1和BA.4/5)以及20例新冠病毒阴性的临床样本(包括11种常见呼吸道病原体),并以测序结果为金标准计算PCR/CRISPR-Cas12a检测方法的ROC曲线下面积(AUC)以及灵敏度(SEN)、特异性(SPE)、一致性(Kappa)评价检测方法的性能。结果本研究筛选到两条特异性crRNA-1和crRNA-2,所建立的方法可在30min内快速特异地检测出SARS-CoV-2奥密克戎BA.4/5变异株,最低检测限为10copies/μL。此外,在检测感染11种常见呼吸道病原体的临床样本时均未观察到非特异性交叉反应。基于crRNA-1和crRNA-2的检测结果的灵敏度分别为97.83%、100%;特异性均为100%;ROC曲线下面积分别为0.9989和1.0;与Sanger测序方法的一致性分别为92.83%、96.41%。结论本研究采用CRISPPR-Cas12a基因编辑技术与逆转录聚合酶链式反应相结合,成功建立了一种快速检测与鉴别SARS-CoV-2奥密克戎BA.4/5变异株的新方法。其特异性强、灵敏度高、稳定性好,可用于新冠病毒奥密克戎BA.4/5变异株的批量检测与分型,可用于常规监测和跟踪SARS-CoV-2变异的传播。

Lactonization of 5-Phenylfuran-4-arylcarbinol-3-carboxylic Acids under the Catalysis of Boron Trifluoridc Etherate

?-Phenyl－4－aroylfuran－3－carboxylic acids were reduced in aqueous alkaline solutions ofNaBH4 to afford hydroxy acids in excellent yields． Under the catalysis of boron trifluoride etherate，the hydroxy acids were converted to lactones smoothly．

A photoactivatable and phenylboronic acid-functionalized nanoassembly for combating multidrug-resistant gram-negative bacteria and their biofilms

Background:Multidrug-resistant(MDR)gram-negative bacteria-related infectious diseases have caused an increase in the public health burden and mortality.Moreover,the formation of biofilms makes these bacteria difficult to control.Therefore,developing novel interventions to combat MDR gram-negative bacteria and their biofilms-related infections are urgently needed.The purpose of this study was to develop a multifunctional nanoassembly(IRNB)based on IR-780 and N,N-di-sec-butyl-N,N-dinitroso-1,4-phenylenediamine(BNN6)for synergistic effect on the infected wounds and subcutaneous abscesses caused by gram-negative bacteria.Methods:The characterization and bacteria-targeting ability of IRNB were investigated.The bac-tericidal efficacy of IRNB against gram-negative bacteria and their biofilms was demonstrated by crystal violet staining assay,plate counting method and live/dead staining in vitro.The antibacterial efficiency of IRNB was examined on a subcutaneous abscess and cutaneous infected wound model in vivo.A cell counting kit-8 assay,Calcein/PI cytotoxicity assay,hemolysis assay and intravenous injection assay were performed to detect the biocompatibility of IRNB in vitro and in vivo.Results:Herein,we successfully developed a multifunctional nanoassembly IRNB based on IR-780 and BNN6 for synergistic photothermal therapy(PTT),photodynamic therapy(PDT)and nitric oxide(NO)effect triggered by an 808 nm laser.This nanoassembly could accumulate specifically at the infected sites of MDR gram-negative bacteria and their biofilms via the covalent coupling effect.Upon irradiation with an 808 nm laser,IRNB was activated and produced both reactive oxygen species(ROS)and hyperthermia.The local hyperthermia could induce NO generation,which further reacted with ROS to generate ONOO−,leading to the enhancement of bactericidal efficacy.Furthermore,NO and ONOO−could disrupt the cell membrane,which converts bacteria to an extremely susceptible state and further enhances the photothermal effect.In this study,IRNB showed a superior photothermal-photodynamic-chemo(NO)synergistic therapeutic effect on the infected wounds and subcutaneous abscesses caused by gram-negative bacteria.This resulted in effective control of associated infections,relief of inflammation,promotion of re-epithelization and collagen deposition,and regulation of angiogenesis during wound healing.Moreover,IRNB exhibited excellent biocompatibility,both in vitro and in vivo.Conclusions:The present research suggests that IRNB can be considered a promising alternative for treating infections caused by MDR gram-negative bacteria and their biofilms.

6-BA Gibberellic Acid 3.6% Liquid HPLC Analysis Method Research

本文采用高效液相色谱法，以甲醇＋甲酸水溶液为流动相，使用ODSC18、51μm为填料的不锈钢柱和二极管阵列检测器。在210nm波长下对赤霉酸·6-BA进行分离和定量分析。结果表明，该分析方法的线性相关系数赤霉酸为O．9999；6-BA为0．998；标准偏差赤霉酸为0．01；6-BA为0．02；变异系数赤霉酸为0．46％；6-BA为0．30％；赤霉酸平均回收率为97．7％；6-BA平均回收率为98．7％。

Bifidobacterium longum CCFM1077 Attenuates Hyperlipidemia by Modulating the Gut Microbiota Composition and Fecal Metabolites:A Randomized,Double-Blind,Placebo-Controlled Clinical Trial

An increasing number of studies have indicated that gut microbiota and its metabolites are crucial in the development of hyperlipidemia.Bifidobacterium longum(B.longum)CCFM1077 has been shown to have lipid-lowering effects in animals.This study aimed to evaluate the potential of B.longum CCFM1077 in lowering the lipid levels in patients with hyperlipidemia and investigate the effect of this bacterium on serum lipid abnormalities,gut microbiota,and fecal metabolites in these patients.This study was a six-week,randomized,double-blind,and placebo-controlled pilot clinical trial.Subjects with hyperlipidemia(N=62)were randomly assigned to receive placebo(N=31)or B.longum CCFM1077(1×1010colony-forming units(CFUs)per day;N=31).Serum lipid levels including total cholesterol(TC),lowdensity lipoprotein cholesterol(LDL-C),total triglyceride(TG),and high-density lipoprotein cholesterol(HDL-C)were examined at the baseline and interventio nal endpoints.Changes in the gut microbiota composition and diversity were measured based on 16S ribosomal RNA(rRNA)sequencing of the V3-V4region at the end of the intervention period.Non-targeted metabolomics of the feces was performed using ultra-performance liquid chromatography(UPLC)-Q-Exactive Orbitrap/mass spectrometer.Oral administration of B.longum CCFM1077 for six weeks significantly decreased the serum levels of TC(p<0.01)and LDL-C(p<0.01)in patients with hyperlipidemia.B.longum CCFM1077 treatment markedly increased gut microbiota diversity and the relative abundance of anti-obesity-related genera,including Lactobacillus,Butyricicoccus,Bifidobacterium,and Blautia,whereas it decreased the relative abundance of obesity-related genera,including Alistipes,Megamonas,and Catenibacterium.Additionally,some key metabolites(bile acids(BAs),biotin,and caffeine)and their corresponding metabolic pathways(primary BA biosynthesis,and taurine and hypotaurine,biotin,purine,and caffeine metabolisms)were enriched by B.longum CCFM1077,and thus it may lower lipid levels.B.longum CCFM1077 is a probiotic strain with the potential to lower serum TC and LDL-C levels patients with hyperlipidemia.The underlying mechanism may be related to the increased abundance of anti-obesity-related genera and fecal metabolites.These findings provide a foundation for future clinical applications of lipid-lowering probiotics in managing individuals with hyperlipidemia.

羟基化氮化硼改性PLA纳米纤维膜的制备及性能研究

为拓展生物基可降解PLA纳米纤维膜在空气过滤等医疗卫生领域的应用范围,通过机械剥离与功能化改性方法制备羟基化氮化硼(BNO)二维纳米片,并采用静电纺丝工艺制备羟基化氮化硼纳米片改性聚乳酸(PLA/BNO)纳米纤维膜。探讨BNO纳米片的引入对PLA纳米纤维膜的微观结构与形貌、导热性能、力学性能以及空气过滤性能的影响。结果表明:与纯PLA纳米纤维膜相比,PLA/BNO纳米纤维膜的拉伸力学性能以及导热性能显著提高。当纺丝液中BNO纳米片质量分数为0.7%时,风速为0.01 m/s时,PLA/BNO纳米纤维膜的过滤效率为99.97%,品质因子达到最大值0.262 Pa^(-1),阻力压降最小为31 Pa,PLA/BNO纳米纤维膜的拉伸断裂强度以及导热系数较纯PLA纳米纤维膜分别提高27.39%和26.62%。

从高钙富硼老卤中提硼研究

以青海某高钙富硼老卤为研究对象开展了提硼研究。结果表明,合适的萃取条件为:磺化煤油为稀释剂、异辛醇用量50%、萃取相比1.0、萃取混合时间15 min;合适的反萃条件为:反萃剂为pH=1的稀盐酸、反萃相比1.5、反萃混合时间15 min。以此条件在5级混合澄清槽中完成了连续逆流萃取—反萃运转试验,连续运转45 h,硼萃取率为95.49%,硼与钾、钠、钙、镁的分离效果较好;硼反萃率为99.59%,反萃液中硼含量达17.17 g/L。采用“溶剂萃取—反萃—高温蒸发—低温冷却结晶—重溶—冷却结晶—过滤洗涤—干燥”工艺高效分离提取硼,硼总回收率为92.33%,制备出的硼酸产品达国家标准(GB/T 538—2018)要求。

高压密闭酸溶-电感耦合等离子体发射光谱法测定硼矿石中的硼

应用电感耦合等离子体发射光谱法(ICP-OES)分析硼矿石中的硼含量,样品分解方法多采用酸溶法和熔融法,硼酸在浓酸溶液中加热蒸发时形成易挥发的BF_(3)或BCl_(3),造成硼的损失。熔融法可分解难溶于酸的硅硼钙石、电气石等样品,但将大量钠盐引入了样品中,基体较大,检出限高。本文建立了高压密闭酸溶、ICP-OES测定硼矿石中硼含量的方法。样品经硝酸和氢氟酸在高压密闭溶样罐中分解完全、定容稀释后,样品溶液用配备耐氢氟酸进样系统的ICP-OES测定。在ICP-OES中,硼有三条常用分析谱线,选取249.677nm为硼的分析谱线,标准曲线的线性相关系数大于0.9995。采用本方法对硼镁矿、锰方硼石和盐湖型固体硼矿三种类型5个不同含量范围的实际样品进行测定,相对标准偏差(RSD,n=11)为0.39%~2.66%,方法检出限为1.76μg/g,硼含量定量范围为5.87μg/g~10.8%。经标准物质验证,硼含量测定值与标准值一致,与容量法和微波消解法测定结果吻合。本方法试剂用量少,无需蒸干样品溶液,有效地避免了硼酸易挥发和试剂用量大的问题。

萘硼酸结构修饰酚醛树脂的合成及其热性能研究

以萘硼酸、苯酚和甲醛水溶液为原料,盐酸和二水合草酸为催化剂,制备了萘硼酸酚醛树脂(BPF)。通过傅里叶变换红外光谱仪、核磁共振分析仪对其进行结构表征,利用差示扫描量热仪考察了升温速率对BPF特征温度的影响,通过热重分析仪、热变形维卡软化点测定仪、电子万能试验机测试了BPF的热性能、软化点和力学性能。结果表明:硼元素以化学键的形式接入到酚醛树脂结构当中;BPF凝胶化温度T_(gel)为113.3℃、固化温度T_(cure)为142.2℃、后处理温度T_(treat)为157.6℃;当萘硼酸含量为10%(wt,质量分数,下同)时,BPF的热分解峰值温度、800℃残炭率比未改性酚醛树脂分别提高了17.39%和28.79%,软化温度提高了16.79%;萘硼酸含量为10%时,BPF拉伸剪切强度、弯曲强度比未改性酚醛树脂分别提高了262.5%和128.3%;萘硼酸含量为6%时,BPF冲击强度提升最大,为2.5kJ/m 2。

6-BA对葡萄果实中有机酸积累及相关基因表达的影响

为探究6-BA对葡萄果实中有机酸积累的影响,及其调控果实有机酸合成的分子机制,以里扎马特葡萄为试材,花前5 d、花后3 d和花后10 d连续3次对花序与果穗进行6-BA处理,研究6-BA对葡萄果实中有机酸积累及其代谢相关基因表达的影响。结果表明,成熟果实中有机酸含量与6-BA处理浓度密切相关,30 mg/L处理后,成熟果实中的酒石酸和总酸含量均显著低于对照,但对苹果酸、柠檬酸和草酸含量并未产生较大影响;对于10,20 mg/L 6-BA处理而言,处理与对照成熟果实中的各种有机酸含量均不存在显著差异。基因表达结果表明,6-BA处理对IDH、MDH和PEPC基因的表达总体表现为抑制,但对于ME基因的表达则主要表现为促进。据此分析,6-BA处理抑制果实中有机酸合成的原因可能与其影响部分有机酸代谢相关基因的表达有关。