[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA con...[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology. [Results]The optimal culture conditions: rooting medium 1/2 MS + 6-BA 0. 24 mg/L + mashed banana 87. 63 g/L + potato 89. 30 g/L + NAA 0. 52 mg/L + sucrose 20. 0 g/L + activated carbon 4. 0 g/L + agar 7. 0 g/L,p H 5. 8,and light intensity 2 000 Lx. [Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D. officinale.展开更多
目的优化俄色叶-A毛囊靶向脂质体的制备工艺。方法薄膜-超声法制备脂质体。以包封率为指标,单因素试验和Box-Behnken效应面法优化制备工艺。结果最佳参数为卵磷脂与胆固醇比例5∶1,卵磷脂与药物比例10∶1,缓冲液用量15 m L。包封率的预...目的优化俄色叶-A毛囊靶向脂质体的制备工艺。方法薄膜-超声法制备脂质体。以包封率为指标,单因素试验和Box-Behnken效应面法优化制备工艺。结果最佳参数为卵磷脂与胆固醇比例5∶1,卵磷脂与药物比例10∶1,缓冲液用量15 m L。包封率的预测值和实测值分别为91.8%和92.3%。脂质体粒子呈球形,粒径为(162.1±6.3)nm,Zeta电位为(-32.8±7.3)m V。结论该制备工艺简单可行,包封率高。展开更多
基金Supported by Scientific and Technological Planning Project of Pu'er City of Yunnan Province(2012kj007)
文摘[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology. [Results]The optimal culture conditions: rooting medium 1/2 MS + 6-BA 0. 24 mg/L + mashed banana 87. 63 g/L + potato 89. 30 g/L + NAA 0. 52 mg/L + sucrose 20. 0 g/L + activated carbon 4. 0 g/L + agar 7. 0 g/L,p H 5. 8,and light intensity 2 000 Lx. [Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D. officinale.
文摘目的优化俄色叶-A毛囊靶向脂质体的制备工艺。方法薄膜-超声法制备脂质体。以包封率为指标,单因素试验和Box-Behnken效应面法优化制备工艺。结果最佳参数为卵磷脂与胆固醇比例5∶1,卵磷脂与药物比例10∶1,缓冲液用量15 m L。包封率的预测值和实测值分别为91.8%和92.3%。脂质体粒子呈球形,粒径为(162.1±6.3)nm,Zeta电位为(-32.8±7.3)m V。结论该制备工艺简单可行,包封率高。
