Glycosides from Erigeron breviscapus

A new glycoside, gamma-pyranone-3-O-beta-D-[6'-(4'-hydroxy-3',5'-dimethyoxybenzoyl)]-glucopyranoside (erigeside D, 2), together with nine known compounds, viz., erigeside I (1), erigeside A (3), erigeside B (4), erigeside II (5), scopolin (6), icariside B-2 (7), blumenol C glucoside (8), (+)-syringaresinol O-beta-D-glucopyranoside (9) and scutellarein-7-O-beta-D-glucuronide methyl ester (10), was isolated from Erigeron breviscapus (Vant.) Hand.-Mazz. Their structures were established on the basis of spectral evidence. According to the beta-glucopyranosyl moiety in 7, the absolute configuration of 7 was determined by X-ray crystallographic analysis. Compounds 7, 8 and 9 were isolated for the first time from this genus, an compound 6 was first obtained from this plant.

Phenolic Compounds from Erigeron breviscapus (Compositae)

Five caffeoyl conjugates were isolated from the ethyl acetate soluble fraction of Erigeron breviscapus (Van.) Hand_Mazz for the first time. Along with them, nine other chemical constituents were also identified. One new caffeoyl conjugate, erigoster A (1) with a novel basic skeleton was mainly assigned by the using a combination of two_dimensional NMR techniques.

A New Glycoside from Erigeron Breviscapus

Erigeside I was isolated from Erigeron breviscapus. The structure elucidation and H-1. C-13 NMR. assignments were achieved by spectroscopic method.

Extraction(DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells

AIMTo investigate the effect of DSX, an active component extracted from Erigeron breviscapus, on the voltage-gated outward K+ channel currents in rat retinal ganglion cells (RGCs) by using electrophysiological method, and to explore the possible mechanisms of DSX on optic nerve protection.METHODSOutward K+ currents were recorded by using whole-cell patch-clamp techniques on acutely isolated rat RGCs. Outward K+ currents were induced by a series of depolarizing voltage pulses from a holding potential of -70 mV to +20 mV in an increment of 10 mV.RESULTSExtracellular application of DSX voltage-dependently suppressed both the steady-state and peak current amplitudes of outward K+ currents in rat RGCs. Furthermore, DSX reversibly and dose-dependently inhibited the amplitudes of outward K+ currents of the cells. At +20 mV membrane potential DSX at the concentrations of 0.02 g/L and 0.05 g/L showed no significant effects on the currents. In contrast, DSX at higher concentrations (0.1 g/L, 0.2 g/L and 0.5 g/L) significantly suppressed the current amplitudes.CONCLUSIONThese results suggest that DSX reversibly and dose-dependently suppress outward K+ channel currents in rat RGCs, which may be one of the possible mechanisms underlying Erigeron breviscapus prevents vision loss and RGC damage caused by glaucoma.

Effect on multifocal electroretinogram in persistently elevated intraocular pressure by erigeron breviscapus extract

AIM: To observe the effect on multifocal electroretinogram (mfERG) in persistently elevated intraocular pressure (TOP) by erigeron breviscapus extract (also named Dengzhanhua in Chinese) in rat models. METHODS: The rat models with persistently elevated TOP were established by the method of Akira. Then, erigeron breviscapus extract was given for one month to observe the effect on mfERG in persistently elevated TOP in rats. RESULTS: As elevated TOP went on, the mfERG changes were mainly in weaken of reaction density with progressive development. After intervention of erigeron breviscapus extract, the total peak latency of 131 wave had recovered to some extent and the difference was significant when compared with control group (P <0.05); the total response density and P1 wave response density in second circle had risen noticeably, which had significant differences than those of control group (P <0.05). CONCLUSION: Erigeron breviscapus extract can improve the impaired visual function of persistently elevated TOP in rats, suggesting that this extract is the effective part of erigeron breviscapus for optic neuroprotection.

The Effect of Erigeron Breviscapus on Proliferation of Pulmonary Artery Smooth Muscle Cells in Hypoxic Porcines

In order to study the effect of Erigeron Breviscapus (EB) on proliferation of pulmonary artery smooth muscle cells (PASMC) in hypoxic porcines, immunohistochemical and MTT methods were employed to measure the proliferation of PASMC. It was found that the proliferation of PASMC in porcines was obvious, and the expression of proliferating cell nuclear antigen (PCNA) was significantly high within 48 h after exposure to hypoxia. The EB could inhibit the proliferation and the expression of PCNA in PASMC under hypoxia, but it had no effect on the proliferation and expression of PCNA in PASMC under normal condition. The EB could inhibit the proliferation and the expression of PCNA in PASMC induced by phorbol 12-myristate 13-acetate (PMA), an agonist of PKC in normal and hypoxic conditions. It was concluded that the hypoxia could enhance the proliferation and expression of PCNA in PASMC. The EB can inhibit the proliferation and expression of PCNA in PASMC under hypoxia through PKC-signal way. The EB may be used in treating the pulmonary hypertension by inhibiting the proliferation of PASMC and the pulmonary vascular remodeling.

Effects of erigeron breviscapus (Vant.) Hand-Mazz pretreatment on pathology and oxyradical level following spinal cord ischemia-reperfusion injury in rabbits

Objective To investigate the effects of erigeron breviscapus (Vant.) Hand-Mazz (erigeron breviscapus) pretreatment on pathology and oxyradical level in the spinal cord after ischemia-reperfusion (I/R) injury in rabbits. Methods A total of 40 New Zealand white rabbits were randomly divided into three groups: sham-operation group with 10 rabbits treated with only abdominal aorta exposure without occlusion,control group with 15 rabbits that underwent ischemia for 50 minutes and treated with matched saline,and experimental group with 15 rabbits that underwent ischemia for 50 minutes and treated with erigeron breviscapus (9mg/kg) injection before ischemia. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in the spinal cord were examined at 6 and 24 hours after I/R,respectively. The morphological changes and the number of the spinal cord anterior horn motor neurons were observed and counted under the light microscope and electron microscope,respectively. Results The level of MDA was markedly decreased and SOD activity was increased in the experimental group compared with those in the control group (P<0.01). Compared with that in the control group,the number of motor neurons in the experimental group significantly increased at 24h after I/R (P<0.01) and the morphous of the motor neurons improved. Conclusion Erigeron breviscapus can reduce oxyradical production and the apoptosis of nerve cells,and protect nerve tissue structure and function after spinal cord I/R.

灯盏花倍性的流式细胞术检测优化

染色体倍性鉴定是植物种质资源评价及多倍体品质选育的重要内容。通过对制样方式、细胞核悬液效果及荧光染料选择等工艺进行优化,建立流式细胞术测定灯盏花倍性的检验体系,并对灯盏花(Erigeron breviscapus Hand.)组培植株进行倍性鉴定。结果表明,采用刀片研磨法制备样品,选择解离液I[45 mmol/L MgCl2,30 mmol/L二水合柠檬酸三钠,20 mmol/L Mops,0.1%(体积比)Triton X-100]进行植物组织材料裂解,并以BioFroxx公司PI染料进行染色,能较好地检验灯盏花的倍性;使用该体系,能检验出组培灯盏花再生植株中的多倍体、混倍体和单倍体。

短葶飞蓬种子活力测定方法的初步研究

[目的]研究短葶飞蓬种子活力的测定方法。[方法]比较种子数目、培养温度对种子发芽的影响,并采用TTC染色测定种子活力。[结果]测得种子出苗率为15.37%,与培养第6天的种子发芽数目比例接近;100和700粒种子发芽试验的重复间发芽率差异较大,300和500粒种子发芽试验的发芽率差异较小;经TTC染色后,短葶飞蓬种子呈现不同类型,可将全部着色和下部着色的种子认定为具有活力的种子,其比例为15.00%,该数值与发芽势和出苗率接近,试验误差较小。[结论]该方法准确可靠,可用于初步测定短葶飞蓬的种子活力。