To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlus...To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlusion(MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus(3.09,6.17,12.34 g/Kg)and Huoxue goups(2.32 g/Kg)when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were(88.43±3.82,69.18±0.11)min,MRT were(138.56±4.83,113.62±2.42)min,and AUC0-twere(28488.35±4800.32,140614.80±23954.05)ng/mL min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were(82.16±1.78,67.08±3.69)min,and MRT were(127.95±2.70,116.58±4.13),AUC0-t were(48619.25±6745.75,159026.00±15003.33)ng/mL min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were(80.29±1.12,69.69±0.87)min and MRT was(128.79±1.46,118.78±4.56)min AUC0-twere(109942.90±13101.83,189417.90±22311.00)ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and brain.展开更多
AIM To develop a new determination method of astrogaloside in anisic acid sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the ...AIM To develop a new determination method of astrogaloside in anisic acid sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the presence of sulfuric acid to determine astragaloside in pig serum. The reaction was allowed to procced on a 60℃ water bath for 20 min. RESULTS The reaction product of astrogaloside and anisic acid has excitation and emission maxima at 320 and 387 nm, respectively. The linear range of determination is from 0 2 to 40 μg·mL -1 . The detection limit is 0 02 μg·mL -1 . The recoveries of samples are between 98 65% and 102 4%. CONCLUSION The proposed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine several kinds of samples successfully.展开更多
目的:从血清降钙素原(procalcitonin,PCT)及同型半胱氨酸(homocysteine,Hcy)变化探讨补阳还五汤(芪龙祛瘀合剂)治疗急性脑梗死患者的疗效机制。方法:选取2018年6月至2020年12月急性脑梗死患者219例,筛选气虚血瘀型患者128例随机分为对照...目的:从血清降钙素原(procalcitonin,PCT)及同型半胱氨酸(homocysteine,Hcy)变化探讨补阳还五汤(芪龙祛瘀合剂)治疗急性脑梗死患者的疗效机制。方法:选取2018年6月至2020年12月急性脑梗死患者219例,筛选气虚血瘀型患者128例随机分为对照组(n=64,西医常规治疗)和治疗组(n=64,西医常规治疗+补阳还五汤)进行临床观察治疗,维持治疗4周。临床上评价2组患者在入院时、入院第10天及入院第4周时的美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分以及中风专用生活质量量表(stroke-specific quality of life scale,SS-QOL)评分变化;并检测3个时间节点患者血清PCT及Hcy水平,统计临床不良反应与实验室不良反应监测。结果:与治疗前相比,治疗4周时2组患者的NIHSS评分均低于治疗前水平,并且治疗组比对照组更低(P<0.01);与治疗前相比,治疗4周时2组患者的SS-QOL评分均高于治疗前,并且治疗组比对照组更高(P<0.01);治疗4周时2组患者Hcy与PCT与同组治疗前比较有统计学差异;并且治疗组患者治疗4周时Hcy与PCT均低于对照组,有统计学差异。结论:补阳还五汤治疗急性脑梗死患者安全有效,可能通过降低血清中PCT及Hcy水平的作用机制,改善神经功能缺损症状。展开更多
基金National Natural Science Foundation of China(Grant No.81373973,81573872,81873228)Science and Technology Planning Project of Guangdong Province(Grant No.2017KZDXM018).
文摘To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlusion(MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus(3.09,6.17,12.34 g/Kg)and Huoxue goups(2.32 g/Kg)when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were(88.43±3.82,69.18±0.11)min,MRT were(138.56±4.83,113.62±2.42)min,and AUC0-twere(28488.35±4800.32,140614.80±23954.05)ng/mL min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were(82.16±1.78,67.08±3.69)min,and MRT were(127.95±2.70,116.58±4.13),AUC0-t were(48619.25±6745.75,159026.00±15003.33)ng/mL min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were(80.29±1.12,69.69±0.87)min and MRT was(128.79±1.46,118.78±4.56)min AUC0-twere(109942.90±13101.83,189417.90±22311.00)ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and brain.
文摘AIM To develop a new determination method of astrogaloside in anisic acid sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the presence of sulfuric acid to determine astragaloside in pig serum. The reaction was allowed to procced on a 60℃ water bath for 20 min. RESULTS The reaction product of astrogaloside and anisic acid has excitation and emission maxima at 320 and 387 nm, respectively. The linear range of determination is from 0 2 to 40 μg·mL -1 . The detection limit is 0 02 μg·mL -1 . The recoveries of samples are between 98 65% and 102 4%. CONCLUSION The proposed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine several kinds of samples successfully.
文摘目的:从血清降钙素原(procalcitonin,PCT)及同型半胱氨酸(homocysteine,Hcy)变化探讨补阳还五汤(芪龙祛瘀合剂)治疗急性脑梗死患者的疗效机制。方法:选取2018年6月至2020年12月急性脑梗死患者219例,筛选气虚血瘀型患者128例随机分为对照组(n=64,西医常规治疗)和治疗组(n=64,西医常规治疗+补阳还五汤)进行临床观察治疗,维持治疗4周。临床上评价2组患者在入院时、入院第10天及入院第4周时的美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分以及中风专用生活质量量表(stroke-specific quality of life scale,SS-QOL)评分变化;并检测3个时间节点患者血清PCT及Hcy水平,统计临床不良反应与实验室不良反应监测。结果:与治疗前相比,治疗4周时2组患者的NIHSS评分均低于治疗前水平,并且治疗组比对照组更低(P<0.01);与治疗前相比,治疗4周时2组患者的SS-QOL评分均高于治疗前,并且治疗组比对照组更高(P<0.01);治疗4周时2组患者Hcy与PCT与同组治疗前比较有统计学差异;并且治疗组患者治疗4周时Hcy与PCT均低于对照组,有统计学差异。结论:补阳还五汤治疗急性脑梗死患者安全有效,可能通过降低血清中PCT及Hcy水平的作用机制,改善神经功能缺损症状。
