Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophy...Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophyll content,soluble protein content and activity of SOD,POD and CAT,and acceleration of MDA accumulation under drought conditions.Low density could effectively improve the grain number per plant,grain weight per plant,1000-grain weight and yield in drought conditions.展开更多
The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total ...The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total phenolic content （TPC）, as well as 1,1 diphenyl-2-picryl hydrazyl （DPPH） free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average ECs0 84.54 μg mL^-1 and IC50 11.54 μg mL^-1 respectively, FTG brans extract had the highest average TPC （24.87 mg GAE g^-1 DW）, and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient （rs） between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively （P〈0.05）. However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.展开更多
Fagopyrum esculentum Moench (buckwheat) is a dicot species from the Polygonaceae family used as a cover crop in agricultural systems featured with a remarkable allelopathic potential for weed control, helping herbicid...Fagopyrum esculentum Moench (buckwheat) is a dicot species from the Polygonaceae family used as a cover crop in agricultural systems featured with a remarkable allelopathic potential for weed control, helping herbicide-resistance management and promoting substantial reductions in herbicide applications. The aim of this research was to examine the allelopathic potential of aqueous extracts from seeds and aerial part of buckwheat on seed germination and initial development of Bidens pilosa and Euphorbia heterophylla. Bioassay experiments were conducted under a completely randomized experimental design with four replications, containing 50 seeds each. Both weed seed species were harvested in a soybean field, and seed viability was previously assessed. Seeds were exposed to four concentrations (0, 25, 50, and 100%) from extracts of seeds (ES) and aerial part (EAP) of buckwheat. Germination speed index (GSI) in B. pilosa and E. heterophylla was daily evaluated throughout 14 and 16 days, respectively, whereas percentage of germination, abnormal seedlings, as well as non-germinated seeds, root (RL) and aerial part length (APL), and total dry matter (TDM) were rated at final germination test. EAP reduced the GSI, especially under the 100% concentration. Germination percentage was lower and abnormal seedlings increased for both weed species when seeds were exposed to EAP concentrations greater than 25%. However, ES did not impinge upon E. heterophylla germination. EAP and ES reduced the APL, RL, and TDM for concentrations greater than 50%, except for ES which did not affect E. heterophylla development. Both extracts from buckwheat have a high capacity to inhibit germination and compromise seedling development, culminating in such a potential alternative for B. pilosa and E. heterophylla management in agricultural systems.展开更多
SGT1(suppressor of the G2 allele of skp1)作为skp1-4的抑制因子,在植物的非生物胁迫响应中具有重要作用。根据甜荞干旱胁迫下的转录组学和蛋白质组学分析,我们克隆出一个与甜荞耐旱性状相关的候选基因FeSGT1。生物信息学分析表明FeS...SGT1(suppressor of the G2 allele of skp1)作为skp1-4的抑制因子,在植物的非生物胁迫响应中具有重要作用。根据甜荞干旱胁迫下的转录组学和蛋白质组学分析,我们克隆出一个与甜荞耐旱性状相关的候选基因FeSGT1。生物信息学分析表明FeSGT1包含一个1086 bp开放阅读框(ORF),编码361个氨基酸,具有3个(TPR、CS和SGS)保守结构域。进化分析表明,FeSGT1与藜麦CqSGT1(XP_021726759.1)、甜菜BvSGT1(XP_010671588.1)和菠菜SoSGT1(XP_021839743.1)亲缘关系较近。亚细胞定位初步显示FeSGT1蛋白定位于细胞膜上。qRT-PCR分析发现FeSGT1在干旱胁迫24 h内表达呈现上调趋势。在盐、低温(4℃)胁迫和ABA处理下,FeSGT1基因表达在12 h达到高峰,24 h后开始下降。在拟南芥中过表达FeSGT1基因发现,在干旱和盐胁迫下,转基因植株的发芽率、根长、鲜重和存活率显著提高,丙二醛(MDA)和过氧化氢(H2O2)含量明显降低,而过氧化氢酶(CAT)活性显著升高。过表达FeSGT1显著增强了转基因拟南芥植株的耐旱和耐盐能力,为深入研究FeSGT1基因调控甜荞抗旱分子机制奠定了基础。展开更多
基金Ningxia Natural Science Foundation(NZ15270)National Agriculture Research System of Oat and Buckwheat(CARS-08-E-5).
文摘Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophyll content,soluble protein content and activity of SOD,POD and CAT,and acceleration of MDA accumulation under drought conditions.Low density could effectively improve the grain number per plant,grain weight per plant,1000-grain weight and yield in drought conditions.
基金supported by the Fundamental Research Funds for the Central Universities,China (XDJK2012B014)
文摘The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total phenolic content （TPC）, as well as 1,1 diphenyl-2-picryl hydrazyl （DPPH） free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average ECs0 84.54 μg mL^-1 and IC50 11.54 μg mL^-1 respectively, FTG brans extract had the highest average TPC （24.87 mg GAE g^-1 DW）, and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient （rs） between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively （P〈0.05）. However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.
文摘Fagopyrum esculentum Moench (buckwheat) is a dicot species from the Polygonaceae family used as a cover crop in agricultural systems featured with a remarkable allelopathic potential for weed control, helping herbicide-resistance management and promoting substantial reductions in herbicide applications. The aim of this research was to examine the allelopathic potential of aqueous extracts from seeds and aerial part of buckwheat on seed germination and initial development of Bidens pilosa and Euphorbia heterophylla. Bioassay experiments were conducted under a completely randomized experimental design with four replications, containing 50 seeds each. Both weed seed species were harvested in a soybean field, and seed viability was previously assessed. Seeds were exposed to four concentrations (0, 25, 50, and 100%) from extracts of seeds (ES) and aerial part (EAP) of buckwheat. Germination speed index (GSI) in B. pilosa and E. heterophylla was daily evaluated throughout 14 and 16 days, respectively, whereas percentage of germination, abnormal seedlings, as well as non-germinated seeds, root (RL) and aerial part length (APL), and total dry matter (TDM) were rated at final germination test. EAP reduced the GSI, especially under the 100% concentration. Germination percentage was lower and abnormal seedlings increased for both weed species when seeds were exposed to EAP concentrations greater than 25%. However, ES did not impinge upon E. heterophylla germination. EAP and ES reduced the APL, RL, and TDM for concentrations greater than 50%, except for ES which did not affect E. heterophylla development. Both extracts from buckwheat have a high capacity to inhibit germination and compromise seedling development, culminating in such a potential alternative for B. pilosa and E. heterophylla management in agricultural systems.
文摘SGT1(suppressor of the G2 allele of skp1)作为skp1-4的抑制因子,在植物的非生物胁迫响应中具有重要作用。根据甜荞干旱胁迫下的转录组学和蛋白质组学分析,我们克隆出一个与甜荞耐旱性状相关的候选基因FeSGT1。生物信息学分析表明FeSGT1包含一个1086 bp开放阅读框(ORF),编码361个氨基酸,具有3个(TPR、CS和SGS)保守结构域。进化分析表明,FeSGT1与藜麦CqSGT1(XP_021726759.1)、甜菜BvSGT1(XP_010671588.1)和菠菜SoSGT1(XP_021839743.1)亲缘关系较近。亚细胞定位初步显示FeSGT1蛋白定位于细胞膜上。qRT-PCR分析发现FeSGT1在干旱胁迫24 h内表达呈现上调趋势。在盐、低温(4℃)胁迫和ABA处理下,FeSGT1基因表达在12 h达到高峰,24 h后开始下降。在拟南芥中过表达FeSGT1基因发现,在干旱和盐胁迫下,转基因植株的发芽率、根长、鲜重和存活率显著提高,丙二醛(MDA)和过氧化氢(H2O2)含量明显降低,而过氧化氢酶(CAT)活性显著升高。过表达FeSGT1显著增强了转基因拟南芥植株的耐旱和耐盐能力,为深入研究FeSGT1基因调控甜荞抗旱分子机制奠定了基础。
