Five new spirostanol saponins(1−5)and seven known compounds(6−12)were isolated from the n-butanol fraction of 75%ethanol extract of Allii Macrostemonis Bulbus.The identification and structural elucidation of all the i...Five new spirostanol saponins(1−5)and seven known compounds(6−12)were isolated from the n-butanol fraction of 75%ethanol extract of Allii Macrostemonis Bulbus.The identification and structural elucidation of all the isolates were performed through extensive 1D and 2D NMR experiments,HR-ESI-MS data analysis and comparisons with literature values.Antioxidant evaluation showed that compounds 6−11 exhibited certain scavenging effects on ABTS radical,where compounds 6,7 and 11 had IC50 values of 0.208,0.057 and 0.014 mg·mL−1,respectively.展开更多
Two new steroidal saponms（2, 3） and two known compounds（1, 4） were isolated from 75% ethanol extract ofAllii macrostemonis bulbus by various spectral methods. The two new steroidal saponins were respectively eluci...Two new steroidal saponms（2, 3） and two known compounds（1, 4） were isolated from 75% ethanol extract ofAllii macrostemonis bulbus by various spectral methods. The two new steroidal saponins were respectively elucidated as （25S）-26-O-β-D-glucopyranosyl-5α-furostanol-2α,3β,22,26-tetrol-3-O-β-D-glucopyranosyl（1→2）-[β-D- glucopyranosyl（1→3）]-β-D-glucopyranosyl（1→4 ）-β-D-galactopyranoside（2 ） and 26-O-β-D-glucopyranosyl-5α- furostanol-25（ 27）-ene-3β,22,26-tetrol-3-O-β-D-glucopyranosyl（ 1→2 ）-[β-D-glucopyranosyl（ 1→3 ）] -β-D-glucopyranosyl- （1→4）-β-D-galactopyranoside（3）. Compound 4 was obtained from this medical plant for the first time. The activity experiments of proliferation inhibition on tumor cells were performed by cell counting kit-8（CCK-8） method for compound 2（25S-configuration） and its isomer（compound 1, 25R-configttration）. The results show that compounds 1 and 2 have weak inhibition on lung cancer A549 cells, melanoma A375 cells and breast cancer MCF-7 cells as well as certain inhibitory effect on liver cancer HepG2 cells, and the inhibitory effect of compound 1 is stronger than that of compound 2.展开更多
基金Jilin Science and Technology Development Program(No.20200404023YY).
文摘Five new spirostanol saponins(1−5)and seven known compounds(6−12)were isolated from the n-butanol fraction of 75%ethanol extract of Allii Macrostemonis Bulbus.The identification and structural elucidation of all the isolates were performed through extensive 1D and 2D NMR experiments,HR-ESI-MS data analysis and comparisons with literature values.Antioxidant evaluation showed that compounds 6−11 exhibited certain scavenging effects on ABTS radical,where compounds 6,7 and 11 had IC50 values of 0.208,0.057 and 0.014 mg·mL−1,respectively.
基金Supported by the National Natural Science Foundation of China（No.31201295）.
文摘Two new steroidal saponms（2, 3） and two known compounds（1, 4） were isolated from 75% ethanol extract ofAllii macrostemonis bulbus by various spectral methods. The two new steroidal saponins were respectively elucidated as （25S）-26-O-β-D-glucopyranosyl-5α-furostanol-2α,3β,22,26-tetrol-3-O-β-D-glucopyranosyl（1→2）-[β-D- glucopyranosyl（1→3）]-β-D-glucopyranosyl（1→4 ）-β-D-galactopyranoside（2 ） and 26-O-β-D-glucopyranosyl-5α- furostanol-25（ 27）-ene-3β,22,26-tetrol-3-O-β-D-glucopyranosyl（ 1→2 ）-[β-D-glucopyranosyl（ 1→3 ）] -β-D-glucopyranosyl- （1→4）-β-D-galactopyranoside（3）. Compound 4 was obtained from this medical plant for the first time. The activity experiments of proliferation inhibition on tumor cells were performed by cell counting kit-8（CCK-8） method for compound 2（25S-configuration） and its isomer（compound 1, 25R-configttration）. The results show that compounds 1 and 2 have weak inhibition on lung cancer A549 cells, melanoma A375 cells and breast cancer MCF-7 cells as well as certain inhibitory effect on liver cancer HepG2 cells, and the inhibitory effect of compound 1 is stronger than that of compound 2.
