Primary thrombocytopenic purpura is ahemorrhagic disease induced by rapid de-struction of blood platelets throughautoimmunity. The authors treated 10 casesof the disease with modified MinorDecoction of Bupleurum with ...Primary thrombocytopenic purpura is ahemorrhagic disease induced by rapid de-struction of blood platelets throughautoimmunity. The authors treated 10 casesof the disease with modified MinorDecoction of Bupleurum with good resultsas reported in the following:展开更多
Saikosaponin v-2(1), was isolated from the roots of the title plant and the structure was identified on the basis of spectral analysis. Saikosaponin v-2 is a new compound, which was identified as 3(,16(,23,28-tetrahyd...Saikosaponin v-2(1), was isolated from the roots of the title plant and the structure was identified on the basis of spectral analysis. Saikosaponin v-2 is a new compound, which was identified as 3(,16(,23,28-tetrahydroxy-olean-11,13(18)-dien-30-oic acid-3-O-(-D-glucopyrano- syl-(1?2)glucopyranosyl-(1?3)-(-D-fucopyranosyl-30-O-xylitol ester.展开更多
[Objectives]This study aimed to evaluate the quality of Bupleurum falcatum Linne interplanted with walnut in Ganluo and study the development and utilization value of other parts.[Methods]The contents of saikosaponins...[Objectives]This study aimed to evaluate the quality of Bupleurum falcatum Linne interplanted with walnut in Ganluo and study the development and utilization value of other parts.[Methods]The contents of saikosaponins a and d were determined with RP-HPLC,and the contents of 7 kinds of harmful elements were determined by ICP-MS.The quality of B.falcatum Linne was compared with that of other Bupleurum species.[Results]The total saikosaponin content in Radix Bupleurum Falcatum(15.894 mg/g)was higher than that in Radix Bupleuri of other origins(8.748 mg/g).The total saikosaponin content in leaves of B.falcatum Linne(7.518 mg/g)is more than twice the limit promulgated by Chinese Pharmacopoeia.The contents of Al,Cr,Cu,As,Cd,Pb and Hg in B.falcatum Linne were all lower than the limits promulgated by the pharmacopoeia.In short,the quality of Radix Bupleurum Falcatum was better than that of Radix Bupleurum Marginatum(S3,S4)and Radix Bupleurum Sachalinense(S5).The leaves of B.falcatum Linne contained more saikosaponins and less harmful elements.[Conclusions]The method and technology of interplanting B.falcatum Linne with walnut in Ganluo are mature.The quality of the medicinal materials produced is superior,and the leaves are also rich in saikosaponins a and d,and can be used as the raw material for extracting saikosaponins a and d.This study provides a basis for further in-depth research on the cultivation of B.falcatum Linne in the domestic market.展开更多
Objective:To explore the mechanism of Bupleurum on fever by means of network pharmacology,and to provide ideas for further experimental study.Methods:The effective components and targets of Xiaochaihu Decoction and Bu...Objective:To explore the mechanism of Bupleurum on fever by means of network pharmacology,and to provide ideas for further experimental study.Methods:The effective components and targets of Xiaochaihu Decoction and Bupleurum were extracted by searching TCM systematic pharmacology database(TCMSP),and got to the effective components and targets of Xiaochaihu Decoction.By searching GeneCards database,disGeNET database,OMIM database and symmap database to extract the disease target of fever.And the"protein interaction network"was constructed with the protein interaction relationship in HINT database as the background network,and the GO enrichment analysis and KEGG pathway enrichment analysis were carried out on the targets by using R software and DAVID database.Results:726 kinds of effective components of Xiaochaihu Decoction were obtained from TCMSP database,including 215 kinds of effective components of Bupleurum,677 targets of Xiaochaihu Decoction and 516 targets of Bupleurum.A total of 7305 fever-related genes were obtained from the disease database,and 400 key targets of Bupleurum for fever treatment were obtained by mapping drug targets and disease targets GAPDH,AKT1,INS,IL-6 and VEGFA,are the targets with high node connectivity in PPI network.The results of GO enrichment of key targets by R software are cofactor binding and coenzyme binding.KEGG enrichment pathway of key targets involves infection,cancer,immune system diseases and so on.Conclusion:This study preliminarily verified the effect and mechanism of Bupleurum on fever,and provided reference for further study.展开更多
[Objectives]To explore the active components and mechanism of Bupleurum and Asarum in the treatment of traumatic brain injury(TBI).[Methods]All the active components and potential action targets of Bupleurum and Asaru...[Objectives]To explore the active components and mechanism of Bupleurum and Asarum in the treatment of traumatic brain injury(TBI).[Methods]All the active components and potential action targets of Bupleurum and Asarum pairs were collected by online platform Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database and literature search.Target genes related to traumatic brain injury were obtained by Online Mendelian Inheritance in Man(OMMI),Therapeutic Target Database(TTD),PharmGKB,Genecards and Drugbank.The"drug-ingredient-target"network diagram was constructed by using Cytoscape software.Venny 2.1.0 was used to integrate the intersection targets of drug targets and disease targets,and the String platform was used to construct a target protein-protein interaction network(PPI).Topological analysis and core target screening of the constructed PPI network were performed using the"CytoNCA"plug-in in Cytoscape software.Gene Ontology(GO)annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the intersection targets using the DAVID database.Finally,Autodock and Pymol software were used to simulate the binding activity of key candidate active components and core genes.[Results]25 active components were screened from Bupleurum-Asarum and 111 potential targets involved in the disease process.GO analysis and KEGG results showed that potential therapeutic targets were mainly enriched in biological processes such as inflammatory response,oxidative stress,cell membrane repair,and cytokine regulation.Network analysis and molecular docking showed that the key compounds of Bupleurum and Asarum were kaempferol and quercetin,which were well docked with the active pockets of four core genes of traumatic brain injury.[Conclusions]Bupleurum and Asarum may be involved in the regulation of inflammatory response,oxidative stress,cell membrane repair through multiple targets and multiple pathways in the treatment of traumatic brain injury.展开更多
A new chromone glycoside, taikochromoside A(1), was isolated from the roots of Bupleurem chinense DC. its spectre was determined on the bases of chemical and spectral analyses.
Minor bupleurum decoction is one of the classic prescriptions of traditional Chinese medicine,and it has obvious effects in the treatment of various diseases.In recent years,the application of minor bupleurum decoctio...Minor bupleurum decoction is one of the classic prescriptions of traditional Chinese medicine,and it has obvious effects in the treatment of various diseases.In recent years,the application of minor bupleurum decoction in the treatment of cancer has attracted more and more attention.This paper describes the mechanism of the role of minor bupleurum decoction in the treatment of clinical tumors and the interaction of related treatments by introducing the composition of minor bupleurum decoction and their pharmacological effects.展开更多
A new isoflavonoside, 7-hydroxy4’-methoxy-isoflavonone-7-0-β-D-glucopyranosyl(1-6)-β -D-glucoppooside was isolated from roots of Bupleurum scorzonerifolium Willd., andnamed as saikoisoflavonoside A. Thestruture was...A new isoflavonoside, 7-hydroxy4’-methoxy-isoflavonone-7-0-β-D-glucopyranosyl(1-6)-β -D-glucoppooside was isolated from roots of Bupleurum scorzonerifolium Willd., andnamed as saikoisoflavonoside A. Thestruture was deteermined by spectroscopic methods.展开更多
A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum a...A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum at five wavelengths (210, 254, 265, 280 and 320 nm) collected by HPLC-DAD instrument was fused to overall control its quality. The fusion fingerprints and four components (saikosaponin a, saikosaponin d, rutin, quercitrin) were studied by systematic quantitative fingerprint method and principal component analysis, respectively. Besides, fingerprint-efficacy relationship was constructed in vitro using partial least-squares model. In conclusion, all work can serve as a valid reference for the quality control of Bupleurum .展开更多
Objective:To observe the clinical efficacy of Shexian bupleurum on insomnia based on evaluation scales and polysomnogram(PSG).Methods:A total of 260 patients suffering from insomnia admitted to the outpatient departme...Objective:To observe the clinical efficacy of Shexian bupleurum on insomnia based on evaluation scales and polysomnogram(PSG).Methods:A total of 260 patients suffering from insomnia admitted to the outpatient department of Handan Central Hospital were included in a randomized,double blind,placebo-controlled trial.The patients were randomly divided into two groups:a control group(receiving placebo,n=150),and an intervention group(receiving Shexian bupleurum treatment,n=110).The clinical trial lasted for 4 weeks.The Pittsburgh Sleep Quality Index(PSQI),Insomnia Severity Index(ISI),Patient Health Questionnaire(PHQ)-9,Generalized Anxiety Disorder(GAD)-7,17 items of Hamilton Depression Scale(HAMD-17),and Hamilton Anxiety Scale(HAMA)were used to evaluate the patients at baseline as well as two weeks and four weeks after treatment;the Treatment Emergent Symptom Scale(TESS)was used to evaluate adverse reactions;polysomnography(PSG)was used to monitor and analyze their sleep characteristics at baseline and four weeks after treatment.Results:The PSQI,ISI,PHQ-9,HAMD-17,and HAMA scores of the intervention group significantly decreased compared to the control group,while the total sleep time,rapid eye movement sleep latency,stage 2 sleep,deep sleep,rapid eye movement sleep,and sleep efficiency of the intervention group significantly increased compared to the control group.The PHQ-9 score of the control group only decreased two weeks after treatment(p<0.05)compared to the intervention group.In addition,there were no obvious adverse events in both the intervention group and the control group.Conclusion:Shexian bupleurum not only improves sleep quality,but also relieves depression and anxiety in patients who suffer from insomnia.展开更多
Bupleurum has been used as traditional medicine in China,Japan,South Korea,and other Asian countries for more than 2,000 years.The crude extracts and pure compounds isolated from Bupleurum have a variety of biological...Bupleurum has been used as traditional medicine in China,Japan,South Korea,and other Asian countries for more than 2,000 years.The crude extracts and pure compounds isolated from Bupleurum have a variety of biological activities,such as anti-inflammatory,anti-cancer,antipyretic,antibacterial,anti-viral,liver protection,neuroprotection,and immune regxilation.The pharmacological effects of Bupleurum have been classified and summarized in this paper by consulting literatures at home and abroad.At the same time,the quality analysis methods of bupleurum,including high-performance liquid chromatography,thin layer chromatography,ultra-high-performance liquid chromatography mass spectrometry,UPLC-QTOF-MS,and so on,are discussed.This paper provides a reference for further research,development,and application of Bupleurum.展开更多
Minor Bupleurum Decoction is a common TCM compound and a classic prescription that can best reflect the"harmonizing method',of TCM.But there are many differences on the usage and dosage of Minor Bupleurum Dec...Minor Bupleurum Decoction is a common TCM compound and a classic prescription that can best reflect the"harmonizing method',of TCM.But there are many differences on the usage and dosage of Minor Bupleurum Decoction.Combined with the clinical treatment experience,this paper summarizes the experience of Minor Bupleurum Decoction in the clinical treatment of diseases.It is hoped that relevant suggestions can be provided for clinicians to improve the clinical therapeutic effect of Minor Bupleurum Decoction.展开更多
A new lignanoside, saikolignanoside A, was isolated from the roots of Bupleurum scorzonerifolium. Its structure was determined as 3-methoxy-4-hydroxy-5-[ (8 S ) -3-methoxy-4- hydroxyphenylpropyl alcohol]-E-cinnamic al...A new lignanoside, saikolignanoside A, was isolated from the roots of Bupleurum scorzonerifolium. Its structure was determined as 3-methoxy-4-hydroxy-5-[ (8 S ) -3-methoxy-4- hydroxyphenylpropyl alcohol]-E-cinnamic alcohol-4-O-b-D-glucopyranoside by means of NMR and MS analysis, acid hydrolysis and optical data.展开更多
This experiment was conducted to evaluate the effects of Bupleurum extract(BE) on blood metabolites, antioxidant status, and immune function in dairy cows under heat stress. Forty lactating Holstein cows were randomly...This experiment was conducted to evaluate the effects of Bupleurum extract(BE) on blood metabolites, antioxidant status, and immune function in dairy cows under heat stress. Forty lactating Holstein cows were randomly assigned to 1 of 4 treatments. The treatments consisted of 0, 0.25, 0.5, and 1.0 g of BE kg–1 dry matter. Supplementation with BE decreased(P<0.05) blood urea nitrogen(BUN) contents and increased blood total protein(TP) and albumin(ALB) levels compared with control cows, but it had no effects(P>0.05) on blood glucose(GLU), nonesterified fatty acid(NEFA), total triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), and high density lipoprotein cholesterol(HDL-C). Compared with control cows, cows fed BE had higher(P<0.05) superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activity. However, supplementation with BE had no effect(P>0.05) on total antioxidant capacity(T-AOC) or malondialdehyde(MDA) levels. The immunoglobulin(Ig) A and G contents increased(P<0.05) in cows fed 0.25 or 0.5 g of BE kg–1. Interleukin(IL)-2 and IL-4 levels were higher(P<0.05) in cows fed 0.5 and 1.0 g of BE kg–1, and IL-6 was significantly elevated(P<0.05) in cows fed 0.5 g of BE kg–1. There were no treatment effects(P>0.05) on the CD4+ and CD8+ T lymphocyte ratios, CD4+/CD8+ ratio, or tumor necrosis factor-α(TNF-α) level among the groups. These findings suggest that BE supplementation may improve protein metabolism, in addition to enhancing antioxidant activity and immune function in heat-stressed dairy cows.展开更多
基金supported by the National Natural Science Foundation of China,Grant No.30670146the National Infrastructure of Natural Resources for Science and Technology,Grant No.2005DKA21403.
文摘Primary thrombocytopenic purpura is ahemorrhagic disease induced by rapid de-struction of blood platelets throughautoimmunity. The authors treated 10 casesof the disease with modified MinorDecoction of Bupleurum with good resultsas reported in the following:
基金This study was financially supported by the National Natural Science Foundation of China (29632050).
文摘Saikosaponin v-2(1), was isolated from the roots of the title plant and the structure was identified on the basis of spectral analysis. Saikosaponin v-2 is a new compound, which was identified as 3(,16(,23,28-tetrahydroxy-olean-11,13(18)-dien-30-oic acid-3-O-(-D-glucopyrano- syl-(1?2)glucopyranosyl-(1?3)-(-D-fucopyranosyl-30-O-xylitol ester.
基金Science and Technology Plan Project of Sichuan Province(2018NZ0113)Technology Research and Development and Promotion and Application Project of Liangshan Prefecture(17YYJS0084)Fundamental Research Funds for the Central Universities(2018NQN13).
文摘[Objectives]This study aimed to evaluate the quality of Bupleurum falcatum Linne interplanted with walnut in Ganluo and study the development and utilization value of other parts.[Methods]The contents of saikosaponins a and d were determined with RP-HPLC,and the contents of 7 kinds of harmful elements were determined by ICP-MS.The quality of B.falcatum Linne was compared with that of other Bupleurum species.[Results]The total saikosaponin content in Radix Bupleurum Falcatum(15.894 mg/g)was higher than that in Radix Bupleuri of other origins(8.748 mg/g).The total saikosaponin content in leaves of B.falcatum Linne(7.518 mg/g)is more than twice the limit promulgated by Chinese Pharmacopoeia.The contents of Al,Cr,Cu,As,Cd,Pb and Hg in B.falcatum Linne were all lower than the limits promulgated by the pharmacopoeia.In short,the quality of Radix Bupleurum Falcatum was better than that of Radix Bupleurum Marginatum(S3,S4)and Radix Bupleurum Sachalinense(S5).The leaves of B.falcatum Linne contained more saikosaponins and less harmful elements.[Conclusions]The method and technology of interplanting B.falcatum Linne with walnut in Ganluo are mature.The quality of the medicinal materials produced is superior,and the leaves are also rich in saikosaponins a and d,and can be used as the raw material for extracting saikosaponins a and d.This study provides a basis for further in-depth research on the cultivation of B.falcatum Linne in the domestic market.
基金National Natural Science Foundation of China(No.81603518)Department of Science and Technology of Shaanxi Province(No.2018ZDXM-SF-008)Xi'an Science and Technology Bureau(No.201805103YX11SF37)。
文摘Objective:To explore the mechanism of Bupleurum on fever by means of network pharmacology,and to provide ideas for further experimental study.Methods:The effective components and targets of Xiaochaihu Decoction and Bupleurum were extracted by searching TCM systematic pharmacology database(TCMSP),and got to the effective components and targets of Xiaochaihu Decoction.By searching GeneCards database,disGeNET database,OMIM database and symmap database to extract the disease target of fever.And the"protein interaction network"was constructed with the protein interaction relationship in HINT database as the background network,and the GO enrichment analysis and KEGG pathway enrichment analysis were carried out on the targets by using R software and DAVID database.Results:726 kinds of effective components of Xiaochaihu Decoction were obtained from TCMSP database,including 215 kinds of effective components of Bupleurum,677 targets of Xiaochaihu Decoction and 516 targets of Bupleurum.A total of 7305 fever-related genes were obtained from the disease database,and 400 key targets of Bupleurum for fever treatment were obtained by mapping drug targets and disease targets GAPDH,AKT1,INS,IL-6 and VEGFA,are the targets with high node connectivity in PPI network.The results of GO enrichment of key targets by R software are cofactor binding and coenzyme binding.KEGG enrichment pathway of key targets involves infection,cancer,immune system diseases and so on.Conclusion:This study preliminarily verified the effect and mechanism of Bupleurum on fever,and provided reference for further study.
基金Supported by Program of Guangxi Natural Science Foundation (2018GXNSFBA050069&2022GXNSFAA035459)the R&D Project of Guangxi Zhuang Autonomous Region Key Trauma Surgery
文摘[Objectives]To explore the active components and mechanism of Bupleurum and Asarum in the treatment of traumatic brain injury(TBI).[Methods]All the active components and potential action targets of Bupleurum and Asarum pairs were collected by online platform Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database and literature search.Target genes related to traumatic brain injury were obtained by Online Mendelian Inheritance in Man(OMMI),Therapeutic Target Database(TTD),PharmGKB,Genecards and Drugbank.The"drug-ingredient-target"network diagram was constructed by using Cytoscape software.Venny 2.1.0 was used to integrate the intersection targets of drug targets and disease targets,and the String platform was used to construct a target protein-protein interaction network(PPI).Topological analysis and core target screening of the constructed PPI network were performed using the"CytoNCA"plug-in in Cytoscape software.Gene Ontology(GO)annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the intersection targets using the DAVID database.Finally,Autodock and Pymol software were used to simulate the binding activity of key candidate active components and core genes.[Results]25 active components were screened from Bupleurum-Asarum and 111 potential targets involved in the disease process.GO analysis and KEGG results showed that potential therapeutic targets were mainly enriched in biological processes such as inflammatory response,oxidative stress,cell membrane repair,and cytokine regulation.Network analysis and molecular docking showed that the key compounds of Bupleurum and Asarum were kaempferol and quercetin,which were well docked with the active pockets of four core genes of traumatic brain injury.[Conclusions]Bupleurum and Asarum may be involved in the regulation of inflammatory response,oxidative stress,cell membrane repair through multiple targets and multiple pathways in the treatment of traumatic brain injury.
文摘A new chromone glycoside, taikochromoside A(1), was isolated from the roots of Bupleurem chinense DC. its spectre was determined on the bases of chemical and spectral analyses.
文摘Minor bupleurum decoction is one of the classic prescriptions of traditional Chinese medicine,and it has obvious effects in the treatment of various diseases.In recent years,the application of minor bupleurum decoction in the treatment of cancer has attracted more and more attention.This paper describes the mechanism of the role of minor bupleurum decoction in the treatment of clinical tumors and the interaction of related treatments by introducing the composition of minor bupleurum decoction and their pharmacological effects.
文摘A new isoflavonoside, 7-hydroxy4’-methoxy-isoflavonone-7-0-β-D-glucopyranosyl(1-6)-β -D-glucoppooside was isolated from roots of Bupleurum scorzonerifolium Willd., andnamed as saikoisoflavonoside A. Thestruture was deteermined by spectroscopic methods.
基金the National Natural Science Foundation of China (Accession no. 81573586, 90612002).
文摘A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum at five wavelengths (210, 254, 265, 280 and 320 nm) collected by HPLC-DAD instrument was fused to overall control its quality. The fusion fingerprints and four components (saikosaponin a, saikosaponin d, rutin, quercitrin) were studied by systematic quantitative fingerprint method and principal component analysis, respectively. Besides, fingerprint-efficacy relationship was constructed in vitro using partial least-squares model. In conclusion, all work can serve as a valid reference for the quality control of Bupleurum .
文摘Objective:To observe the clinical efficacy of Shexian bupleurum on insomnia based on evaluation scales and polysomnogram(PSG).Methods:A total of 260 patients suffering from insomnia admitted to the outpatient department of Handan Central Hospital were included in a randomized,double blind,placebo-controlled trial.The patients were randomly divided into two groups:a control group(receiving placebo,n=150),and an intervention group(receiving Shexian bupleurum treatment,n=110).The clinical trial lasted for 4 weeks.The Pittsburgh Sleep Quality Index(PSQI),Insomnia Severity Index(ISI),Patient Health Questionnaire(PHQ)-9,Generalized Anxiety Disorder(GAD)-7,17 items of Hamilton Depression Scale(HAMD-17),and Hamilton Anxiety Scale(HAMA)were used to evaluate the patients at baseline as well as two weeks and four weeks after treatment;the Treatment Emergent Symptom Scale(TESS)was used to evaluate adverse reactions;polysomnography(PSG)was used to monitor and analyze their sleep characteristics at baseline and four weeks after treatment.Results:The PSQI,ISI,PHQ-9,HAMD-17,and HAMA scores of the intervention group significantly decreased compared to the control group,while the total sleep time,rapid eye movement sleep latency,stage 2 sleep,deep sleep,rapid eye movement sleep,and sleep efficiency of the intervention group significantly increased compared to the control group.The PHQ-9 score of the control group only decreased two weeks after treatment(p<0.05)compared to the intervention group.In addition,there were no obvious adverse events in both the intervention group and the control group.Conclusion:Shexian bupleurum not only improves sleep quality,but also relieves depression and anxiety in patients who suffer from insomnia.
文摘Bupleurum has been used as traditional medicine in China,Japan,South Korea,and other Asian countries for more than 2,000 years.The crude extracts and pure compounds isolated from Bupleurum have a variety of biological activities,such as anti-inflammatory,anti-cancer,antipyretic,antibacterial,anti-viral,liver protection,neuroprotection,and immune regxilation.The pharmacological effects of Bupleurum have been classified and summarized in this paper by consulting literatures at home and abroad.At the same time,the quality analysis methods of bupleurum,including high-performance liquid chromatography,thin layer chromatography,ultra-high-performance liquid chromatography mass spectrometry,UPLC-QTOF-MS,and so on,are discussed.This paper provides a reference for further research,development,and application of Bupleurum.
文摘Minor Bupleurum Decoction is a common TCM compound and a classic prescription that can best reflect the"harmonizing method',of TCM.But there are many differences on the usage and dosage of Minor Bupleurum Decoction.Combined with the clinical treatment experience,this paper summarizes the experience of Minor Bupleurum Decoction in the clinical treatment of diseases.It is hoped that relevant suggestions can be provided for clinicians to improve the clinical therapeutic effect of Minor Bupleurum Decoction.
文摘A new lignanoside, saikolignanoside A, was isolated from the roots of Bupleurum scorzonerifolium. Its structure was determined as 3-methoxy-4-hydroxy-5-[ (8 S ) -3-methoxy-4- hydroxyphenylpropyl alcohol]-E-cinnamic alcohol-4-O-b-D-glucopyranoside by means of NMR and MS analysis, acid hydrolysis and optical data.
基金supported financially by the National Key Research and Development Program of China (2016YFD0500503)the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS12)the Anhui International Science and Technology Cooperation Plan Program, China (1503062019)
文摘This experiment was conducted to evaluate the effects of Bupleurum extract(BE) on blood metabolites, antioxidant status, and immune function in dairy cows under heat stress. Forty lactating Holstein cows were randomly assigned to 1 of 4 treatments. The treatments consisted of 0, 0.25, 0.5, and 1.0 g of BE kg–1 dry matter. Supplementation with BE decreased(P<0.05) blood urea nitrogen(BUN) contents and increased blood total protein(TP) and albumin(ALB) levels compared with control cows, but it had no effects(P>0.05) on blood glucose(GLU), nonesterified fatty acid(NEFA), total triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), and high density lipoprotein cholesterol(HDL-C). Compared with control cows, cows fed BE had higher(P<0.05) superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activity. However, supplementation with BE had no effect(P>0.05) on total antioxidant capacity(T-AOC) or malondialdehyde(MDA) levels. The immunoglobulin(Ig) A and G contents increased(P<0.05) in cows fed 0.25 or 0.5 g of BE kg–1. Interleukin(IL)-2 and IL-4 levels were higher(P<0.05) in cows fed 0.5 and 1.0 g of BE kg–1, and IL-6 was significantly elevated(P<0.05) in cows fed 0.5 g of BE kg–1. There were no treatment effects(P>0.05) on the CD4+ and CD8+ T lymphocyte ratios, CD4+/CD8+ ratio, or tumor necrosis factor-α(TNF-α) level among the groups. These findings suggest that BE supplementation may improve protein metabolism, in addition to enhancing antioxidant activity and immune function in heat-stressed dairy cows.
