Root of burdock contains high amounts of dietary fibers and polyphenols. To improve the functional properties, the root was fermented with Aspergillus awamori. Effect of the fermented burdock on alloxan-induced mouse ...Root of burdock contains high amounts of dietary fibers and polyphenols. To improve the functional properties, the root was fermented with Aspergillus awamori. Effect of the fermented burdock on alloxan-induced mouse diabetes was examined. A diet containing the 5% fermented burdock powers was prepared to examine effect of the burdock diet on alloxan-induced mouse diabetes. Mice fed the burdock diet and the control diet for 14 weeks. Then, alloxan (200 mg/kg of body weight) was administrated to each mouse. After 5 days from the administration, blood glucose assay and glucose tolerance test were carried out. Incidence of hyperglycemia decreased and the glucose metabolism was improved when mice fed the burdock diet. Insulin, C-peptide, biomarkers of oxidative stress in plasma and apoptosis in pancreas were examined and compared to those obtained from mice fed the control diet. It is deduced that alloxan-induced diabetes is caused to lower insulin concentration. The fermented-burdock diet improves the diabetes and prevents apoptosis in the pancreas.展开更多
In the present study, we investigated the induction of volatile organic compounds （VOCs） of Lycoperslcon esculentum Mill. and its resistance to Botrytis cinerea Pers. by burdock oligosaccharlde. The disease severity...In the present study, we investigated the induction of volatile organic compounds （VOCs） of Lycoperslcon esculentum Mill. and its resistance to Botrytis cinerea Pers. by burdock oligosaccharlde. The disease severity of L. esculentum was evaluated 48 h after treatment with 0.6% burdock oligosaccharlde, followed by inoculation with a spore suspension of B. cinerea. The formation of O2＇, the activity of lipoxygenases （LOX）, peroxidase （POD）, cataiase （CAT）, and superoxide dismutase （SOD）, and the quantity and quallty of changes In VOCs were determined a period of time after treatment with 0.6% burdock ollgosaccharide. The results demonstrated that the disease index in treated plants was decreased by 42.5% compared with control 96 h after Inoculation. The production of O2＇ reached a maximum 6 h after treatment （1.36-fold compared with control）. There was an increase in LOX, POD, CAT and SOD activity in response to burdock oligosaccharide treatment and the enzymes showed different trends in the time-course of induction. At 120 h after treatment, （E）-2-hexenal was increased by 92% compared with control, whereas methyl salicylate showed a gradual Increase with induction period. Previous results had demonstrated that chitosan elicitor enhanced the production VOCs of L. esculentum and decreased plant susceptibility towards B. clnerea. Together, these findings suggest that increasing the production of VOCs in response to burrdock oligosaccharide may be an important mechanism for L. esculentumin its defense against pathogens, in addition, burrdock oligosaccharlde may act as a potent elicitor of resistance to disease in L. esculentum.展开更多
文摘Root of burdock contains high amounts of dietary fibers and polyphenols. To improve the functional properties, the root was fermented with Aspergillus awamori. Effect of the fermented burdock on alloxan-induced mouse diabetes was examined. A diet containing the 5% fermented burdock powers was prepared to examine effect of the burdock diet on alloxan-induced mouse diabetes. Mice fed the burdock diet and the control diet for 14 weeks. Then, alloxan (200 mg/kg of body weight) was administrated to each mouse. After 5 days from the administration, blood glucose assay and glucose tolerance test were carried out. Incidence of hyperglycemia decreased and the glucose metabolism was improved when mice fed the burdock diet. Insulin, C-peptide, biomarkers of oxidative stress in plasma and apoptosis in pancreas were examined and compared to those obtained from mice fed the control diet. It is deduced that alloxan-induced diabetes is caused to lower insulin concentration. The fermented-burdock diet improves the diabetes and prevents apoptosis in the pancreas.
文摘In the present study, we investigated the induction of volatile organic compounds （VOCs） of Lycoperslcon esculentum Mill. and its resistance to Botrytis cinerea Pers. by burdock oligosaccharlde. The disease severity of L. esculentum was evaluated 48 h after treatment with 0.6% burdock oligosaccharlde, followed by inoculation with a spore suspension of B. cinerea. The formation of O2＇, the activity of lipoxygenases （LOX）, peroxidase （POD）, cataiase （CAT）, and superoxide dismutase （SOD）, and the quantity and quallty of changes In VOCs were determined a period of time after treatment with 0.6% burdock ollgosaccharide. The results demonstrated that the disease index in treated plants was decreased by 42.5% compared with control 96 h after Inoculation. The production of O2＇ reached a maximum 6 h after treatment （1.36-fold compared with control）. There was an increase in LOX, POD, CAT and SOD activity in response to burdock oligosaccharide treatment and the enzymes showed different trends in the time-course of induction. At 120 h after treatment, （E）-2-hexenal was increased by 92% compared with control, whereas methyl salicylate showed a gradual Increase with induction period. Previous results had demonstrated that chitosan elicitor enhanced the production VOCs of L. esculentum and decreased plant susceptibility towards B. clnerea. Together, these findings suggest that increasing the production of VOCs in response to burrdock oligosaccharide may be an important mechanism for L. esculentumin its defense against pathogens, in addition, burrdock oligosaccharlde may act as a potent elicitor of resistance to disease in L. esculentum.
