Objective: From molecular, cell and systemic clinical treatment three levels, this report revealed the mechanism of wound healing, the basic theory and the importance of clinical practice for the Burn Regenerative The...Objective: From molecular, cell and systemic clinical treatment three levels, this report revealed the mechanism of wound healing, the basic theory and the importance of clinical practice for the Burn Regenerative Therapy with MEBT/MEBO (BRT & MEBT/MEBO).Method: This report analyzed the importance and clinical curative effect for standardized appliance of BRT & MEBT/MEBO from two key points: 1) Liquefaction of necrotic tissue from burn wound; 2) Skin regeneration in situ . Result: There are three necessary conditions for skin physiologically repair and regeneration of burn wound: 1) The formation of moist physiological environment on burn wound; 2) The material foundation of life regenerative substances and histology for keratin-19 stem cells’ regeneration in situ ; 3) Standardized procedures and appliance of BRT & MEBT/MEBO, which is the guarantee of burn wound healing physiologically. Conclusion: The theory of “Potential Regenerative Cell" and the technique of “Stem cell in situ regeneration" are the basic theory and clinical treatment for BRT. Full-thickness burn skin can be healed physiologically and the skin tissues and organs can be regenerated ONLY by standardized procedures and timely appliance of BRT & MEBT/MEBO.展开更多
Burns,with their high incidence and mortality rates,have a devastating effect on patients.There are still huge challenges in the management of burns.Mesenchymal stem cells(MSCs),which have multidirectional differentia...Burns,with their high incidence and mortality rates,have a devastating effect on patients.There are still huge challenges in the management of burns.Mesenchymal stem cells(MSCs),which have multidirectional differentiation potential,have aroused interest in exploring the capacity for treating different intractable diseases due to their strong proliferation,tissue repair,immune tolerance and paracrine abilities,among other features.Currently,several animal studies have shown that MSCs play various roles and have beneficial effects in promoting wound healing,inhibiting burn inflammation and preventing the formation of pathological scars during burn healing process.The substances MSCs secrete can act on peripheral cells and promote burn repair.According to preclinical research,MSC-based treatments can effectively improve burn wound healing and reduce pain.However,due to the small number of patients and the lack of controls,treatment plans and evaluation criteria vary widely,thus limiting the value of these clinical studies.Therefore,to better evaluate the safety and effectiveness of MSC-based burn treatments,standardization of the application scheme and evaluation criteria of MSC therapy in burn treatment is required in the future.In addition,the combination of MSC pretreatment and dressing materials are also conducive to improving the therapeutic effect of MSCs on burns.In this article,we review current animal research and clinical trials based on the use of stem cell therapy for treating burns and discuss the main challenges and coping strategies facing future clinical applications.展开更多
文摘Objective: From molecular, cell and systemic clinical treatment three levels, this report revealed the mechanism of wound healing, the basic theory and the importance of clinical practice for the Burn Regenerative Therapy with MEBT/MEBO (BRT & MEBT/MEBO).Method: This report analyzed the importance and clinical curative effect for standardized appliance of BRT & MEBT/MEBO from two key points: 1) Liquefaction of necrotic tissue from burn wound; 2) Skin regeneration in situ . Result: There are three necessary conditions for skin physiologically repair and regeneration of burn wound: 1) The formation of moist physiological environment on burn wound; 2) The material foundation of life regenerative substances and histology for keratin-19 stem cells’ regeneration in situ ; 3) Standardized procedures and appliance of BRT & MEBT/MEBO, which is the guarantee of burn wound healing physiologically. Conclusion: The theory of “Potential Regenerative Cell" and the technique of “Stem cell in situ regeneration" are the basic theory and clinical treatment for BRT. Full-thickness burn skin can be healed physiologically and the skin tissues and organs can be regenerated ONLY by standardized procedures and timely appliance of BRT & MEBT/MEBO.
基金supported by the National Natural Science Foundation of China(Grant No.31771065)Sichuan Provincial Science and Technology Program(Grant No.2019JDRC0020)the 1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(Grant No.ZYJC18002).
文摘Burns,with their high incidence and mortality rates,have a devastating effect on patients.There are still huge challenges in the management of burns.Mesenchymal stem cells(MSCs),which have multidirectional differentiation potential,have aroused interest in exploring the capacity for treating different intractable diseases due to their strong proliferation,tissue repair,immune tolerance and paracrine abilities,among other features.Currently,several animal studies have shown that MSCs play various roles and have beneficial effects in promoting wound healing,inhibiting burn inflammation and preventing the formation of pathological scars during burn healing process.The substances MSCs secrete can act on peripheral cells and promote burn repair.According to preclinical research,MSC-based treatments can effectively improve burn wound healing and reduce pain.However,due to the small number of patients and the lack of controls,treatment plans and evaluation criteria vary widely,thus limiting the value of these clinical studies.Therefore,to better evaluate the safety and effectiveness of MSC-based burn treatments,standardization of the application scheme and evaluation criteria of MSC therapy in burn treatment is required in the future.In addition,the combination of MSC pretreatment and dressing materials are also conducive to improving the therapeutic effect of MSCs on burns.In this article,we review current animal research and clinical trials based on the use of stem cell therapy for treating burns and discuss the main challenges and coping strategies facing future clinical applications.
