目的观察电针足阳明经四白穴诱导大鼠孤束核(nucleus of solitary tract,NTS)的原癌基因c—fos表达,探讨四白穴与胃的延髓初级中枢联系。方法采用抗FOS蛋白的免疫组织化学方法(SABC法)。结果电针刺激“四白”穴组,在延髓的孤束核(NTS)...目的观察电针足阳明经四白穴诱导大鼠孤束核(nucleus of solitary tract,NTS)的原癌基因c—fos表达,探讨四白穴与胃的延髓初级中枢联系。方法采用抗FOS蛋白的免疫组织化学方法(SABC法)。结果电针刺激“四白”穴组,在延髓的孤束核(NTS)、迷走神经背核(DMNX)、三叉神经脊束核尾侧亚核(nSpVc)、最后区(AP)、延髓腹外侧区(VLM)及网状结构(RF)中可发现FOS样免疫反应(Fos—like—immunoreactivity,FLI)阳性神经元,为双侧分布,无明显侧别差异。在NTS内有广泛的FLI神经元,且在孤束内侧亚核(mnTS)内FLI神经元数目电针四白组与非经非穴组比较,差异有非常显著性意义(P<0.01)。结论提示电针足阳明经四白穴,通过激活延髓内与内脏信息相关的中枢核团神经元来调节胃功能。展开更多
Elemental analyzer/isotope ratio mass spectrometry(EA/TRMS) has been widely applied to analyze the^(15)N/^(14)N isotope composition(δ^(15)N) of plants and soils,but the δ^(15)N results may be inaccurate due to incom...Elemental analyzer/isotope ratio mass spectrometry(EA/TRMS) has been widely applied to analyze the^(15)N/^(14)N isotope composition(δ^(15)N) of plants and soils,but the δ^(15)N results may be inaccurate due to incomplete combustion of the high-C/N-ratio plant samples by EA.Therefore,it is necessary to develop a method to solve the problem of imperfect combustion.In this study,we used two methods:1) adding copper oxide powder to the samples,and 2) increasing the O_2 flow(from 100 mL min^(-1) to 200 mL min^(-1)) for the auto sampler inlet purge line of the EA.The δ^(15)N values of the plant samples became more positive and tended to be stable after complete combustion.Also,the required blank samples for each plant sample decreased with increasing amount of the added CuO powder.However,at 200 mL min^(-1) of the oxygen flow in the EA,complete combustion could not be achieved without adding copper oxide,but this was done with decreased amount of CuO powder.Therefore,mixing cupric oxide into the high-C/N-ratio samples was an efficient,simple and convenient way to solve the problem of imperfect combustion in the EA.展开更多
Subjective: To investigate the mechanisms of electroacupuncture (EA) in regulating the gastric activity in rats. Methods: 64 SD rats were randomly divided into group A and B, both of which were further divided into EA...Subjective: To investigate the mechanisms of electroacupuncture (EA) in regulating the gastric activity in rats. Methods: 64 SD rats were randomly divided into group A and B, both of which were further divided into EA subgroup a and b (n=8×2), sciatotomy+EA subgroup a and b (n=8×2), vagotomy+EA subgroup a and b (n=8×2) and control subgroup a and b (n=8×2). Rats of the 4 subgroups of group A were used for observing changes of c fos expression, while those of the 4 subgroups of group B used for analyzing changes of electrogastrogram (EGG). Left Zusanli (ST 36) was punctured and stimulated for 30 min using a 6805 A EA Therapeutic Apparatus by setting the parameters as intermittent waves, frequency of 50 Hz and strength of 20 V. Changes of c fos expression in the nucleus of tractus solitarius (NTS) and dorsal motor nucleus of vagus (DMV) of the medullary bulb were detected by using immunohistochemical method and the effect of EA on EGG using electrophysiological method in 846 mixture anesthetized rats. Results: After EA of Zusanli(ST 36), the expression of c fos in NTS and DMV changed significantly and marked changes were observed in the gastric electro activity. Conclusion: Electro acupuncture can regulate gastric activity by activating neurons of NTS and DMV.展开更多
subjective: The effect of 4～5 Hz electroacupuncture (EA) on alterations of both met enkephalin (MEK) and dynorphin (Dyn) in the patient plasma or mouse spinal cord and its relation with analgesic effect were studied....subjective: The effect of 4～5 Hz electroacupuncture (EA) on alterations of both met enkephalin (MEK) and dynorphin (Dyn) in the patient plasma or mouse spinal cord and its relation with analgesic effect were studied.Methods: In acupuncture clinic 10 patients with acute pain were treated with 4 Hz EA at Zusanli(ST 36) and/or Hegu(LI 4) acupoints for 30 min. 20 BALB/C mice were randomly divided into 2 groups: a. EA group(n=10), treated with 4～5 Hz EA at bilateral “Zusanli"(ST 36) for 15 min; b. control group(n=10) treated with no EA, but also restrained for 15 min. Before and after EA or restraining acupoints, the pain threshold of the patients or mice was detected. 10 μl of the patient plasma before and after EA and each mouse spinal cord suspension, of the 2 groups were blotted onto nitrocellulose membrane (NCM) respectively. The protein dot blot signals were detected by immunoreactivity (IR) and using Shimadu TLC Scanner and analyzed statistically. Results: The results showed that an increase in patient plasma MEK IR or Dyn IR and a decrease in mouse spinal MEK IR or Dyn IR could be detected, and the alteration of plasma or spinal MEK IR was more significant than that of plasma or spinal Dyn IR. There was a positive correlation in alteration between plasma or spinal MEK IR and plasma or spinal Dyn IR with respective parallel levels in individuals. The increased plasma MEK IR or the decreased spinal MEK IR was positively or negatively correlated with the analgesic effect, while the correlation between plasma or spinal Dyn IR and analgesic effect was insignificant. Conclusion: The results suggest that under lower frequency EA the met enkephalin may play an important role in analgesia.展开更多
To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymp...To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymphocyte cfosmRNA, ppENKmRNA, MEKIR and DynIR was studied. 20 BALB/C mice were randomly divided into 2 groups: a) acupuncture group, treated with 5 Hz electroacupuncture(EA); b) control group, treated with restraint but no EA. The mouse pain threshold was detected by K+ ionophoresis before and after EA or restraining. The mouse lymphocyte cfosmRNA and ppENKmRNA were demonstrated by in situ hybridization and RNA dot blot. The mouse lymphocyte MEKIR and DynIR were detected by immunohistochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that all the signals offosmRNA, ppENKmRNA, MEKIR and DynIR were localized in the lymphocyte cytoplasma and stronger in the acupuncture group than that in the control group(P<0.05). All of the signals were positively correlated with the analgesic effect in the EA group(P<0.05-0.01). Besides, there was a positive correlation on alteration between cfosmRNA and ppENKmRNA or between cfosmRNA and DynIR(P<0.05), suggesting that cFos may be involved in ppE or ppD gene transcription in the mouse lymphocyte.展开更多
文摘目的观察电针足阳明经四白穴诱导大鼠孤束核(nucleus of solitary tract,NTS)的原癌基因c—fos表达,探讨四白穴与胃的延髓初级中枢联系。方法采用抗FOS蛋白的免疫组织化学方法(SABC法)。结果电针刺激“四白”穴组,在延髓的孤束核(NTS)、迷走神经背核(DMNX)、三叉神经脊束核尾侧亚核(nSpVc)、最后区(AP)、延髓腹外侧区(VLM)及网状结构(RF)中可发现FOS样免疫反应(Fos—like—immunoreactivity,FLI)阳性神经元,为双侧分布,无明显侧别差异。在NTS内有广泛的FLI神经元,且在孤束内侧亚核(mnTS)内FLI神经元数目电针四白组与非经非穴组比较,差异有非常显著性意义(P<0.01)。结论提示电针足阳明经四白穴,通过激活延髓内与内脏信息相关的中枢核团神经元来调节胃功能。
文摘Elemental analyzer/isotope ratio mass spectrometry(EA/TRMS) has been widely applied to analyze the^(15)N/^(14)N isotope composition(δ^(15)N) of plants and soils,but the δ^(15)N results may be inaccurate due to incomplete combustion of the high-C/N-ratio plant samples by EA.Therefore,it is necessary to develop a method to solve the problem of imperfect combustion.In this study,we used two methods:1) adding copper oxide powder to the samples,and 2) increasing the O_2 flow(from 100 mL min^(-1) to 200 mL min^(-1)) for the auto sampler inlet purge line of the EA.The δ^(15)N values of the plant samples became more positive and tended to be stable after complete combustion.Also,the required blank samples for each plant sample decreased with increasing amount of the added CuO powder.However,at 200 mL min^(-1) of the oxygen flow in the EA,complete combustion could not be achieved without adding copper oxide,but this was done with decreased amount of CuO powder.Therefore,mixing cupric oxide into the high-C/N-ratio samples was an efficient,simple and convenient way to solve the problem of imperfect combustion in the EA.
文摘Subjective: To investigate the mechanisms of electroacupuncture (EA) in regulating the gastric activity in rats. Methods: 64 SD rats were randomly divided into group A and B, both of which were further divided into EA subgroup a and b (n=8×2), sciatotomy+EA subgroup a and b (n=8×2), vagotomy+EA subgroup a and b (n=8×2) and control subgroup a and b (n=8×2). Rats of the 4 subgroups of group A were used for observing changes of c fos expression, while those of the 4 subgroups of group B used for analyzing changes of electrogastrogram (EGG). Left Zusanli (ST 36) was punctured and stimulated for 30 min using a 6805 A EA Therapeutic Apparatus by setting the parameters as intermittent waves, frequency of 50 Hz and strength of 20 V. Changes of c fos expression in the nucleus of tractus solitarius (NTS) and dorsal motor nucleus of vagus (DMV) of the medullary bulb were detected by using immunohistochemical method and the effect of EA on EGG using electrophysiological method in 846 mixture anesthetized rats. Results: After EA of Zusanli(ST 36), the expression of c fos in NTS and DMV changed significantly and marked changes were observed in the gastric electro activity. Conclusion: Electro acupuncture can regulate gastric activity by activating neurons of NTS and DMV.
文摘subjective: The effect of 4～5 Hz electroacupuncture (EA) on alterations of both met enkephalin (MEK) and dynorphin (Dyn) in the patient plasma or mouse spinal cord and its relation with analgesic effect were studied.Methods: In acupuncture clinic 10 patients with acute pain were treated with 4 Hz EA at Zusanli(ST 36) and/or Hegu(LI 4) acupoints for 30 min. 20 BALB/C mice were randomly divided into 2 groups: a. EA group(n=10), treated with 4～5 Hz EA at bilateral “Zusanli"(ST 36) for 15 min; b. control group(n=10) treated with no EA, but also restrained for 15 min. Before and after EA or restraining acupoints, the pain threshold of the patients or mice was detected. 10 μl of the patient plasma before and after EA and each mouse spinal cord suspension, of the 2 groups were blotted onto nitrocellulose membrane (NCM) respectively. The protein dot blot signals were detected by immunoreactivity (IR) and using Shimadu TLC Scanner and analyzed statistically. Results: The results showed that an increase in patient plasma MEK IR or Dyn IR and a decrease in mouse spinal MEK IR or Dyn IR could be detected, and the alteration of plasma or spinal MEK IR was more significant than that of plasma or spinal Dyn IR. There was a positive correlation in alteration between plasma or spinal MEK IR and plasma or spinal Dyn IR with respective parallel levels in individuals. The increased plasma MEK IR or the decreased spinal MEK IR was positively or negatively correlated with the analgesic effect, while the correlation between plasma or spinal Dyn IR and analgesic effect was insignificant. Conclusion: The results suggest that under lower frequency EA the met enkephalin may play an important role in analgesia.
文摘To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymphocyte cfosmRNA, ppENKmRNA, MEKIR and DynIR was studied. 20 BALB/C mice were randomly divided into 2 groups: a) acupuncture group, treated with 5 Hz electroacupuncture(EA); b) control group, treated with restraint but no EA. The mouse pain threshold was detected by K+ ionophoresis before and after EA or restraining. The mouse lymphocyte cfosmRNA and ppENKmRNA were demonstrated by in situ hybridization and RNA dot blot. The mouse lymphocyte MEKIR and DynIR were detected by immunohistochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that all the signals offosmRNA, ppENKmRNA, MEKIR and DynIR were localized in the lymphocyte cytoplasma and stronger in the acupuncture group than that in the control group(P<0.05). All of the signals were positively correlated with the analgesic effect in the EA group(P<0.05-0.01). Besides, there was a positive correlation on alteration between cfosmRNA and ppENKmRNA or between cfosmRNA and DynIR(P<0.05), suggesting that cFos may be involved in ppE or ppD gene transcription in the mouse lymphocyte.
