Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with human...Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.展开更多
objective:To seek a good method for plasma disinfection to solve the serious problem of viral con tamination of blood and blood products. Methods: A model of plasma disinfection was established by using ultraviolet C ...objective:To seek a good method for plasma disinfection to solve the serious problem of viral con tamination of blood and blood products. Methods: A model of plasma disinfection was established by using ultraviolet C (UVC) irradiation and cross-linked starch iodine (CSI) treatment to kill Sindbis virus (SV). The efficacy of plasma virus inactivation was measured by assay of cell infection. The plasma IgG activity was surveyed using different immunohistochemical assays. Results: After being irradiated by 986O J/m2 UVC fol lowed by passage through a column of CSl with a velocity of 0. 25 ml/min, the SV in plasma was reduced to 6. 8 log. No significant inhibition of the activities of the antigen and antibody of IgG were found in the plas ma. Couclusion: The use of UVC in combination with CSI can effectively inactivate SV in the plasma. Thus it is a useful measure for the disinfection of blood products.展开更多
We report fabrication and characterization of organic heterojunction UV detectors based on N,N＇-bis（naphthalen- 1-y1）-N,N＇-bis （phenyl） benzidine （NPB） and fullerene C60. The effects of different thicknesses o...We report fabrication and characterization of organic heterojunction UV detectors based on N,N＇-bis（naphthalen- 1-y1）-N,N＇-bis （phenyl） benzidine （NPB） and fullerene C60. The effects of different thicknesses of NPB and C60 layers are studied and compared. Notably, the optimal thicknesses of electron acceptor C60 and electron donor NPB are 40 nm and 80 nm, respectively. The J V characteristic curves of the device demonstrate a three-order- of-magnitude difference when illuminated under a 350nm UV light and in the dark at -0.5 V. The device exhibits high sensitivity in the region of 320-380nm with the peak located around 35Onm. Especially, it shows excellent photo-response characteristic with a responsivity as high as 315 mA/W under the illumination of 192μW.cm 2 350nm UV light at -5 V. These results indicate that the NPB/C60 heterojunction structure device might be used as low-cost low-voltage UV photodetectors.展开更多
A ten-month field research study was meticulously conducted at Robert Moses State Park (RMSP) on the south shore of Long Island, NY. The objective was to determine if aerial phenomena of an unknown nature exist over a...A ten-month field research study was meticulously conducted at Robert Moses State Park (RMSP) on the south shore of Long Island, NY. The objective was to determine if aerial phenomena of an unknown nature exist over a coastal location and to characterize their properties and behaviors. Primary and secondary field observation methods were utilized in this data-centric study. Forensic engineering principles and methodologies guided the study. The challenges set forward were object detection, observation, and characterization, where multispectral electro-optical devices and radar were employed due to limited visual acuity and intermittent presentation of the phenomena. The primary means of detection utilized a 3 cm X-band radar operating in two scan geometries, the X- and Y-axis. Multispectral electro-optical devices were utilized as a secondary means of detection and identification. Data was emphasized using HF and LF detectors and spectrum analyzers incorporating EM, ultrasonic, magnetic, and RF field transducers to record spectral data in these domains. Data collection concentrated on characterizing VIS, NIR, SWIR, LWIR, UVA, UVB, UVC, and the higher energy spectral range of ionizing radiation (alpha, beta, gamma, and X-ray) recorded by Geiger-Müller counters as well as special purpose semiconductor diode sensors.展开更多
为了研究短波紫外线处理对桃果实采后酚类物质代谢和贮藏品质的影响,本试验以玉露桃果实为材料,研究了3.0 k J·m-2UV-C处理对桃果实在25℃8 d贮藏期间酚类物质代谢关键酶活性和果实抗氧化能力的影响。结果表明,3.0 k J·m-2UV-...为了研究短波紫外线处理对桃果实采后酚类物质代谢和贮藏品质的影响,本试验以玉露桃果实为材料,研究了3.0 k J·m-2UV-C处理对桃果实在25℃8 d贮藏期间酚类物质代谢关键酶活性和果实抗氧化能力的影响。结果表明,3.0 k J·m-2UV-C处理能显著抑制桃果实采后可滴定酸含量的下降,抑制果实维生素C含量的损失,增强果实采后着色能力,从而保持果实的感官和食用品质。进一步的研究发现,3.0 k J·m-2UV-C处理诱导了果实PAL活性的升高,增强了果实贮藏初期苯丙烷代谢强度,增加了酚类物质的积累。另外,UV-C处理抑制了贮藏后期PPO和POD活性,减轻了由PPO和POD参与的酚类物质的酶促氧化,减少了桃果实中抗氧化组分含量的下降,维持了果实贮藏期间较高的DPPH自由基清除能力。可见适宜剂量的UV-C处理(3.0 k J·m-2)在桃果实采后贮运保鲜和抗氧化能力调控中具有潜在的应用价值。展开更多
以红心萝卜为实验材料,研究了不同剂量的短波紫外线(ultraviolet-C,UV-C)处理对鲜切红心萝卜的抗氧化系统和抗氧化活性的影响。鲜切红心萝卜用0、0.25、0.5、1.0、2.0 k J/m2剂量的UV-C处理后于5℃贮藏48 h。结果表明,1.0 k J/m2 UV-C...以红心萝卜为实验材料,研究了不同剂量的短波紫外线(ultraviolet-C,UV-C)处理对鲜切红心萝卜的抗氧化系统和抗氧化活性的影响。鲜切红心萝卜用0、0.25、0.5、1.0、2.0 k J/m2剂量的UV-C处理后于5℃贮藏48 h。结果表明,1.0 k J/m2 UV-C处理能最有效地诱导鲜切红心萝卜中酚类物质的合成,进而导致其含量增加,并提高了红心萝卜中的1,1-二苯基-2-三硝基苯肼自由基清除能力。UV-C处理能促进鲜切红心萝卜中花青素和黄酮类物质含量的增加、延缓抗坏血酸含量的下降、并提高抗氧化酶活性。这些结果表明,UV-C处理可以诱导酚类物质合成,提高抗氧化酶活性,从而显著提升鲜切红心萝卜的抗氧化活性。展开更多
文摘Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.
文摘objective:To seek a good method for plasma disinfection to solve the serious problem of viral con tamination of blood and blood products. Methods: A model of plasma disinfection was established by using ultraviolet C (UVC) irradiation and cross-linked starch iodine (CSI) treatment to kill Sindbis virus (SV). The efficacy of plasma virus inactivation was measured by assay of cell infection. The plasma IgG activity was surveyed using different immunohistochemical assays. Results: After being irradiated by 986O J/m2 UVC fol lowed by passage through a column of CSl with a velocity of 0. 25 ml/min, the SV in plasma was reduced to 6. 8 log. No significant inhibition of the activities of the antigen and antibody of IgG were found in the plas ma. Couclusion: The use of UVC in combination with CSI can effectively inactivate SV in the plasma. Thus it is a useful measure for the disinfection of blood products.
基金Supported by the National Natural Science Foundation of China under Grant Nos 61274068 and 61404058the Project of Science and Technology Development Plan of Jilin Province under Grant Nos 20150204003GX and 20130206021GXthe Project of Science and Technology Plan of Changchun City under Grant No 14KG020
文摘We report fabrication and characterization of organic heterojunction UV detectors based on N,N＇-bis（naphthalen- 1-y1）-N,N＇-bis （phenyl） benzidine （NPB） and fullerene C60. The effects of different thicknesses of NPB and C60 layers are studied and compared. Notably, the optimal thicknesses of electron acceptor C60 and electron donor NPB are 40 nm and 80 nm, respectively. The J V characteristic curves of the device demonstrate a three-order- of-magnitude difference when illuminated under a 350nm UV light and in the dark at -0.5 V. The device exhibits high sensitivity in the region of 320-380nm with the peak located around 35Onm. Especially, it shows excellent photo-response characteristic with a responsivity as high as 315 mA/W under the illumination of 192μW.cm 2 350nm UV light at -5 V. These results indicate that the NPB/C60 heterojunction structure device might be used as low-cost low-voltage UV photodetectors.
文摘A ten-month field research study was meticulously conducted at Robert Moses State Park (RMSP) on the south shore of Long Island, NY. The objective was to determine if aerial phenomena of an unknown nature exist over a coastal location and to characterize their properties and behaviors. Primary and secondary field observation methods were utilized in this data-centric study. Forensic engineering principles and methodologies guided the study. The challenges set forward were object detection, observation, and characterization, where multispectral electro-optical devices and radar were employed due to limited visual acuity and intermittent presentation of the phenomena. The primary means of detection utilized a 3 cm X-band radar operating in two scan geometries, the X- and Y-axis. Multispectral electro-optical devices were utilized as a secondary means of detection and identification. Data was emphasized using HF and LF detectors and spectrum analyzers incorporating EM, ultrasonic, magnetic, and RF field transducers to record spectral data in these domains. Data collection concentrated on characterizing VIS, NIR, SWIR, LWIR, UVA, UVB, UVC, and the higher energy spectral range of ionizing radiation (alpha, beta, gamma, and X-ray) recorded by Geiger-Müller counters as well as special purpose semiconductor diode sensors.
文摘为了研究短波紫外线处理对桃果实采后酚类物质代谢和贮藏品质的影响,本试验以玉露桃果实为材料,研究了3.0 k J·m-2UV-C处理对桃果实在25℃8 d贮藏期间酚类物质代谢关键酶活性和果实抗氧化能力的影响。结果表明,3.0 k J·m-2UV-C处理能显著抑制桃果实采后可滴定酸含量的下降,抑制果实维生素C含量的损失,增强果实采后着色能力,从而保持果实的感官和食用品质。进一步的研究发现,3.0 k J·m-2UV-C处理诱导了果实PAL活性的升高,增强了果实贮藏初期苯丙烷代谢强度,增加了酚类物质的积累。另外,UV-C处理抑制了贮藏后期PPO和POD活性,减轻了由PPO和POD参与的酚类物质的酶促氧化,减少了桃果实中抗氧化组分含量的下降,维持了果实贮藏期间较高的DPPH自由基清除能力。可见适宜剂量的UV-C处理(3.0 k J·m-2)在桃果实采后贮运保鲜和抗氧化能力调控中具有潜在的应用价值。
文摘以红心萝卜为实验材料,研究了不同剂量的短波紫外线(ultraviolet-C,UV-C)处理对鲜切红心萝卜的抗氧化系统和抗氧化活性的影响。鲜切红心萝卜用0、0.25、0.5、1.0、2.0 k J/m2剂量的UV-C处理后于5℃贮藏48 h。结果表明,1.0 k J/m2 UV-C处理能最有效地诱导鲜切红心萝卜中酚类物质的合成,进而导致其含量增加,并提高了红心萝卜中的1,1-二苯基-2-三硝基苯肼自由基清除能力。UV-C处理能促进鲜切红心萝卜中花青素和黄酮类物质含量的增加、延缓抗坏血酸含量的下降、并提高抗氧化酶活性。这些结果表明,UV-C处理可以诱导酚类物质合成,提高抗氧化酶活性,从而显著提升鲜切红心萝卜的抗氧化活性。
