The fruits of leguminous plants Cercis Chinensis Bunge are still overlooked although they have been reported to be antioxidative because of the limited information on the phytochemicals of C.chinensis fruits.A simple,...The fruits of leguminous plants Cercis Chinensis Bunge are still overlooked although they have been reported to be antioxidative because of the limited information on the phytochemicals of C.chinensis fruits.A simple,rapid and sensitive HPLC-MS/MS method was developed for the identification and quantitation of the major bioactive components in C.chinensis fruits.Eighteen polyphenols were identified,which are first reported in C.chinensis fruits.Moreover,ten components were simultaneously quantified.The validated quantitative method was proved to be sensitive,reproducible and accurate.Then,it was applied to analyze batches of C.chinensis fruits from different phytomorph and areas.The principal components analysis(PCA)realized visualization and reduction of data set dimension while the hierarchical cluster analysis(HCA)indicated that the content of phenolic acids or all ten components might be used to differentiate C.chinensis fruits of different phytomorph.展开更多
Prepared in this experiment were six groups of diets, i.e. VC0, VC1, VC2, VC3, VC4 and VC5 with the contents of vitamin C (VC mg(100 g) -1 diet) of 0, 100, 200, 400, 800, and 1200 respectively. It was found that vitam...Prepared in this experiment were six groups of diets, i.e. VC0, VC1, VC2, VC3, VC4 and VC5 with the contents of vitamin C (VC mg(100 g) -1 diet) of 0, 100, 200, 400, 800, and 1200 respectively. It was found that vitamin C increased the concentrations of immunoglobulin-like (IgG-like, IgA-like and IgM-like) substances in the serum of Penaeus chinensis after a feeding period of 3 weeks. The differences among groups were significant (P<0.01), but there was no diffe- rence in the contents of complement3-like and complement4-like substances in the serum (P>0.05). Phenoloxidase (PO) activity in the serum of VC3 group shrimps was higher than that of VC0 and other groups, but no significant difference was observed between VC0 group and other groups. Furthermore, bactericidal activity of the serum to Vibrio parahaemolyticus in shrimps fed with the VC1 diet was higher than that in the other groups (P<0.01), while no difference was demonstrated among all groups for the bactericidal activity to Vibrio alginolyticus (P>0.05). It is, therefore, suggested that vitamin C (100-400 mg(100 g) -1 diets) could be used as an immunostimulant of P. chinensis.展开更多
A new bidesmoside triterpenoid saponin, named stauntoside C1 （1） has been isolated from Stauntonia chinensis. Its structure was established by means of spectral and chemical methods. C 2009 Guo Ping Zhou. Published ...A new bidesmoside triterpenoid saponin, named stauntoside C1 （1） has been isolated from Stauntonia chinensis. Its structure was established by means of spectral and chemical methods. C 2009 Guo Ping Zhou. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
Two new isomeric compounds of rare acyclic C13-norisoprenoids, along with two known lignans sauchinone and licarin A, were isolated from the herb of Saururus chinensis （Lour.） Baill. Their structures were elucidated...Two new isomeric compounds of rare acyclic C13-norisoprenoids, along with two known lignans sauchinone and licarin A, were isolated from the herb of Saururus chinensis （Lour.） Baill. Their structures were elucidated on the basis of spectral analysis.展开更多
[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium ...[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid （ 12：6：3：3：1 ） as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography （TLC）, and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol （5：1） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.展开更多
Nine enclosures (5 m x 5 m) were built in a Fenneropenaeus chinensis culture pond of Rushan Gulf in April, 2001. The probiotics and BIO ENERGIZER solution were applied for disparate treatments. Variations of alkaline ...Nine enclosures (5 m x 5 m) were built in a Fenneropenaeus chinensis culture pond of Rushan Gulf in April, 2001. The probiotics and BIO ENERGIZER solution were applied for disparate treatments. Variations of alkaline phosphatase ac- tivity (APA) and its relationship with the contents of C, N and P in sediments were studied. Results show that APA of sedi- ments increases from 3.096 nmolg-1min-1 to 5.407 nmol g-1min-1 in culture period; the bacteria biomass is not the only factor to determine APA; the contents of total P and total organic carbon have a significant positive correlation with APA, while that of total nitrogen has a negative correlation. In addition, the contents of inorganic P and organic P are not regular with APA. By comparison, TOC shows a more significant coherence with APA, meaning that organic pollution in sediments af- fects APA remarkably.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.82073808,81872828,and 81573384)。
文摘The fruits of leguminous plants Cercis Chinensis Bunge are still overlooked although they have been reported to be antioxidative because of the limited information on the phytochemicals of C.chinensis fruits.A simple,rapid and sensitive HPLC-MS/MS method was developed for the identification and quantitation of the major bioactive components in C.chinensis fruits.Eighteen polyphenols were identified,which are first reported in C.chinensis fruits.Moreover,ten components were simultaneously quantified.The validated quantitative method was proved to be sensitive,reproducible and accurate.Then,it was applied to analyze batches of C.chinensis fruits from different phytomorph and areas.The principal components analysis(PCA)realized visualization and reduction of data set dimension while the hierarchical cluster analysis(HCA)indicated that the content of phenolic acids or all ten components might be used to differentiate C.chinensis fruits of different phytomorph.
文摘Prepared in this experiment were six groups of diets, i.e. VC0, VC1, VC2, VC3, VC4 and VC5 with the contents of vitamin C (VC mg(100 g) -1 diet) of 0, 100, 200, 400, 800, and 1200 respectively. It was found that vitamin C increased the concentrations of immunoglobulin-like (IgG-like, IgA-like and IgM-like) substances in the serum of Penaeus chinensis after a feeding period of 3 weeks. The differences among groups were significant (P<0.01), but there was no diffe- rence in the contents of complement3-like and complement4-like substances in the serum (P>0.05). Phenoloxidase (PO) activity in the serum of VC3 group shrimps was higher than that of VC0 and other groups, but no significant difference was observed between VC0 group and other groups. Furthermore, bactericidal activity of the serum to Vibrio parahaemolyticus in shrimps fed with the VC1 diet was higher than that in the other groups (P<0.01), while no difference was demonstrated among all groups for the bactericidal activity to Vibrio alginolyticus (P>0.05). It is, therefore, suggested that vitamin C (100-400 mg(100 g) -1 diets) could be used as an immunostimulant of P. chinensis.
文摘A new bidesmoside triterpenoid saponin, named stauntoside C1 （1） has been isolated from Stauntonia chinensis. Its structure was established by means of spectral and chemical methods. C 2009 Guo Ping Zhou. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
文摘Two new isomeric compounds of rare acyclic C13-norisoprenoids, along with two known lignans sauchinone and licarin A, were isolated from the herb of Saururus chinensis （Lour.） Baill. Their structures were elucidated on the basis of spectral analysis.
基金Supported by Technical Innovation Project of Shandong Province(201210916001)
文摘[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid （ 12：6：3：3：1 ） as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography （TLC）, and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol （5：1） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.
基金the Major State Basic Research of China(Grant No.G1999012011) the National High Technology Research and Development Program of China(2002AA648010).
文摘Nine enclosures (5 m x 5 m) were built in a Fenneropenaeus chinensis culture pond of Rushan Gulf in April, 2001. The probiotics and BIO ENERGIZER solution were applied for disparate treatments. Variations of alkaline phosphatase ac- tivity (APA) and its relationship with the contents of C, N and P in sediments were studied. Results show that APA of sedi- ments increases from 3.096 nmolg-1min-1 to 5.407 nmol g-1min-1 in culture period; the bacteria biomass is not the only factor to determine APA; the contents of total P and total organic carbon have a significant positive correlation with APA, while that of total nitrogen has a negative correlation. In addition, the contents of inorganic P and organic P are not regular with APA. By comparison, TOC shows a more significant coherence with APA, meaning that organic pollution in sediments af- fects APA remarkably.
