The performance of solid solution aging treatment on aluminum matrix composites prepared by powder metallurgy and reinforced with 6061 aluminum alloy powder as matrix;meanwhile, nano silicon carbide particles(nm Si Cp...The performance of solid solution aging treatment on aluminum matrix composites prepared by powder metallurgy and reinforced with 6061 aluminum alloy powder as matrix;meanwhile, nano silicon carbide particles(nm Si Cp), submicron silicon carbide particles(1 μm Si Cp) and Ti particles were studied. The Al/Si Cp composite powder was prepared by high-energy ball milling, and then cold-pressed, sintered, hotextruded, and then heat-treated with different solution temperatures and aging times for the extruded composites. Optical microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy(EDS), X-ray diffractometer(XRD) and extrusion testing were used to analyze and test the microstructure and mechanical properties of aluminum matrix composites. The results show that after the multi-stage solid solution at 530 ℃×2 h+535 ℃×2 h+540 ℃×2 h, the particles are mainly equiaxed grains and uniformly distributed. There is no reinforcement agglomeration, and the surface is dense and the insoluble phase is basically dissolved. In the matrix, the strengthening effect is good, and the hardness and compressive strength are 179.43 HV and 680.42 MPa, respectively. Under this solution process, when the aluminum matrix composites are aged at 170 ℃ for 10 h, the hardness and compressive strength can reach their peaks and increase to 195.82 HV and 721.48 MPa, respectively.展开更多
Serum levels of cartilage oligomeric matrix protein(COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has als...Serum levels of cartilage oligomeric matrix protein(COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has also been associated with hepatocellular carcinoma. We present a prospective analysis of this biomarker in38 patients with chronic hepatitis C who were subject to eradication therapy with direct acting antivirals. We confirm previous studies associating COMP elevation with liver cirrhosis. We also show how viral levels are correlated with COMP at baseline. In our prospective analysis, we report that successful eradication of hepatitis C results in improvement in liver stiffness and laboratory liver function tests at 1 year follow-up. In contrast, median COMP-levels remain unchanged during the study period. We conclude that the biomarker potential of COMP in the prospective evaluation of liver diseases, remains to be elucidated.展开更多
BACKGROUND Matrix metalloproteinases(MMPs)participate in the degradation of extracellular matrix compounds,maintaining the homeostasis between fibrogenesis and fibrolytic processes in the liver.However,there are few s...BACKGROUND Matrix metalloproteinases(MMPs)participate in the degradation of extracellular matrix compounds,maintaining the homeostasis between fibrogenesis and fibrolytic processes in the liver.However,there are few studies on the regulation of liver MMPs in fibrosis progression in humans.AIM To assess the production activity and regulation of matrix metalloproteinases in liver fibrosis stages in chronic hepatitis C(CHC).METHODS A prospective,cross-sectional,multicenter study was conducted.CHC patients were categorized in fibrosis grades through FibroTest®and/or FibroScan®.Serum MMP-2,-7,and-9 were determined by western blot and multiplex suspension array assays.Differences were validated by the Kruskal-Wallis and Mann-Whitney U tests.The Spearman correlation coefficient and area under the receiver operating characteristic curve were calculated.Collagenolytic and gelatinase activity was determined through the Azocoll substrate and zymogram test,whereas tissue inhibitor of metalloproteinase-1 production was determined by dot blot assays.RESULTS Serum concentrations of the MMPs evaluated were higher in CHC patients than in healthy subjects.MMP-7 distinguished early and advanced stages,with a correlation of 0.32(P<0.001),and the area under the receiver operating characteristic displayed moderate sensitivity and specificity for MMP-7 in F4(area under the receiver operating characteristic,0.705;95%confidence interval:0.605-0.805;P<0.001).Collagenolytic activity was detected at F0 and F1,whereas gelatinase activity was not detected at any fibrosis stage.Tissue inhibitor of metalloproteinase-1 determination showed upregulation in F0 and F1 but downregulation in F2(P<0.001).CONCLUSION High concentrations of inactive MMPs were present in the serum of CHC patients,reflecting the impossibility to restrain liver fibrosis progression.MMPs could be good diagnostic candidates and therapeutic targets for improving novel strategies to reverse liver fibrosis in CHC.展开更多
In order to improve wear resistance and decrease the cost, carbon and carbon nanotubes reinforced copper matrix composites were fabricated by the power metallurgy method. The effects of carbon （carbon and carbon nano...In order to improve wear resistance and decrease the cost, carbon and carbon nanotubes reinforced copper matrix composites were fabricated by the power metallurgy method. The effects of carbon （carbon and carbon nanotubes） volume fraction and applied load on the friction coefficient and wear rate under dry sliding of the composites were investigated at room temperature. By scanning electron microscopy （SEM）, the worn surfaces and debris were observed, and wear mechanism was also analyzed and discussed. The experimental wear process consists of the run-in, steady wear and severe wear process with the increasing of sliding distance. Both the friction coefficient and wear rate of the composites first decrease and then increase with the increasing of carbon volume fraction. The minimum friction coefficient and wear rate are obtained when carbon is 4.0vo1%. The wear mechanisms of the composites change from the adhesive wear and delamination wear to abrasive wear with the increasing of carbon volume fraction.展开更多
We derive necessary and sufficient conditions for the existence of a Hermitian nonnegative-definite solution to the matrix equation AXB = C. Moreover, we derive a representation of a general Hermitian nonnegative-defi...We derive necessary and sufficient conditions for the existence of a Hermitian nonnegative-definite solution to the matrix equation AXB = C. Moreover, we derive a representation of a general Hermitian nonnegative-definite solution. We then apply our solution to two examples, including a comparison of our solution to a proposed solution by Zhang in [1] using an example problem given from [1]. Our solution demonstrates that the proposed general solution from Zhang in [1] is incorrect. We also give a second example in which we derive the general covariance structure so that two matrix quadratic forms are independent.展开更多
Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, a...Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, and bone sialoprotein （BSP） were detected by immunohistochemistry in C2C12 cultured from day 1 to day 28. Results The signaling of bone matrix protein expression became weaker except for type I collagen, OC and BSP after 5 days. Fourteen days after culture, the positive signaling of type I collagen, OPN, ON, OC, and BSP was gradually declined, and could be detected significantly as compared with that of the negative control on day 28. BMP assay showed that the lkaline phosphatase （ALP） activity was higher in C2C12 culture than in the control during the 14-day culture. Also, total protein and DNA significantly increased during the 14-day culture. High levels of ALP were seen in preosteoblasts and osteoblsts in vivo and in differentiating osteoblasts in vitro. ALP was well recognized as a marker reflecting osteoblastic activity. Conclusion Native bovine BMP induces conversion of myoblasts into osteoblasts, produces type I collagen, and plays significantly role in osteoinduction and bone matrix mineralization of C2C 12 in vitro.展开更多
The selection of milling tools for Si C14Cu4Mg0.5Si based on Aluminium matrix 2A14 was analyzed, and the factors that affect the efficiency of the milling were discussed. The Si C14Cu4Mg0.5Si was designed for use on t...The selection of milling tools for Si C14Cu4Mg0.5Si based on Aluminium matrix 2A14 was analyzed, and the factors that affect the efficiency of the milling were discussed. The Si C14Cu4Mg0.5Si was designed for use on the moon landing vehicle or missile wings, but the hardness of aluminium-silicon carbide composite material was very high, much higher than the general hardness of cemented carbide, which will bring many difficulties in the aluminium-silicon carbide composite material processing. The chemical compositions of Si C14Cu4Mg0.5Si were analyzed. A new selected indexable cutter was designed to mill Si C14Cu4Mg0.5Si. The structure design of milling cutter was different from the conventional milling cutter, breaking the previous limitations to a certain extent, pioneering the idea. The tool material wear was detected by experiments. The mechanical and physical properties of Si C14Cu4Mg0.5Si were also tested. Si C14Cu4Mg0.5Si exhibited different surface quality characteristics under different milling tools.展开更多
Cr3 C2-NiCr particles were injected into the melted surface of Q235 low carbon steel to make a surface metal matrix composite （MMC） layer by gas tungsten are melt injection （GTAMI） process. Hardness of the surface...Cr3 C2-NiCr particles were injected into the melted surface of Q235 low carbon steel to make a surface metal matrix composite （MMC） layer by gas tungsten are melt injection （GTAMI） process. Hardness of the surface MMC layer was tested. Wear resistance of the surface MMC was investigated with a ball-on-disk dry sliding setup. Microstrnetures of the surface MMC layer and morphology of the worn surfaces were investigated with scanning electron microscopy （SEM）. The results showed that the hardness of the MMC was as high as 1 960. 7 HV. Wear loss of the upper part of the MMC layer is onlyO. 8% of that of the substrate under the dry sliding condition given. Wear loss of the bottom part is 2. 5 % of that of the substrate.展开更多
The photoionization cross section of the ground state 2s22p 2Po1/2 and the first excited state 2s22p2po1/2 of C II ions are systematically calculated using the fully relativistic R-matrix code DARC. The detailed reson...The photoionization cross section of the ground state 2s22p 2Po1/2 and the first excited state 2s22p2po1/2 of C II ions are systematically calculated using the fully relativistic R-matrix code DARC. The detailed resonances are presented and identified for the photon energy ranging from threshold （24.38 eV） up to 41.5 eV where the L-shell （2p, 2s） photoionization process is dominant. In the calculations, the relativistic effect and electronic correlation effect are well considered. It is found that the relativistic effect is very important for the light atomic system CII, which accounts for experimentally observed fine structure resonance peaks. A careful comparison is made between the present results and the experimental values, and also other theoretical data available in the literature, showing that good agreement is obtained for the resonance peaks.展开更多
Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. Contrary to normal cells, proteins of this group are preferentially phosphorylated. Phosphoproteins of hepat...Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. Contrary to normal cells, proteins of this group are preferentially phosphorylated. Phosphoproteins of hepatoma nuclear matrix are selectively subjected to rapid proteolysis. By alkali treatment and a monoclonal antibody against phosphotyrosyl residue the presence of two high molecular weight bands of phosphotyrosyl-containing proteins was detected in nuclear matrices of tumor but not of normal liver cells. High molecular weight protein group of tumor nuclear matrices revealed also a rapid turnover and preferential incorporation of labeled amino acids selectively inhibited by chloramphenicol.展开更多
Objective： Nuclear matrix protein is tissue, cell-type specific, and tumor-relative. It plays an important role in the regulation of intranuclear processes. Some researches also showed that a c-erbB-2 promoter-specif...Objective： Nuclear matrix protein is tissue, cell-type specific, and tumor-relative. It plays an important role in the regulation of intranuclear processes. Some researches also showed that a c-erbB-2 promoter-specific DNA-binding nuclear matrix protein is present only in malignant human breast tissues and induces mitogenesis and cell surface expression of the c-erbB-2 protein in resting NIH/3T3 cells. But it is not clear that how it in colon adenocarcinomas. Methods： Two-dimensional gel electrophoretic method was used for NMP identification and immunohistochemistry was used for c-erbB-2 detection in 12 cases of colon adenocarcinomas and matched adjacent normal colon tissues. Results： 5 different nuclear matrix proteins （named C1-C5） were identified in 12 colon adenocarcinoma specimens, but not in the matched adjacent normal colon tissues; 3 nuclear matrix proteins （named N1-N3） were identified in all 12 matched adjacent normal colon tissues, but not in colon adenocarcinoma specimens. A nuclear matrix protein （named N4） was detected in all of 9 moderated-well differentiated adenocarcinomas and all 12 matched adjacent normal colon tissues, but not in 3 poor-differentiated adenocarcinomas. All of the 10 colon adenocarcinomas which had the nuclear matrix protein C4 were c-erbB-2 expression positive. Conclusion： The data suggest that there are specific nuclear matrix proteins in colon adenocarcinomas and its subtypes, which maybe valuable to serve as markers of colon adenocarcinomas in future. Nuclear matrix protein C4 probably is a c-erbB-2 promotor-specific nuclear matrix protein in colon adenocarcinomas, and may induce the expression of c-erbB-2.展开更多
AIM: To evaluate the high sensitivity C-reactive protein(hs CRP), Fetuin-A and matrix γ-carboxyglutamate protein(MGP) as the main factors for vascular calcification and inflammation in serum of patients with advanced...AIM: To evaluate the high sensitivity C-reactive protein(hs CRP), Fetuin-A and matrix γ-carboxyglutamate protein(MGP) as the main factors for vascular calcification and inflammation in serum of patients with advanced age-related macular degeneration(ARMD) in comparison to healthy controls. METHODS: The subjects were 40 patients with choroidal neovascularization(CNV) having a mean age of70.9 ±9.1y and a matched group of 49 apparently healthy control subjects. The ARMD was diagnosed using a slitlamp with superfield lens, fundus photography and fluorescein angiography. Measurement of hs CRP was done by nephelometry method. Levels of Fetuin-A and MGP were measured by enzyme-linked immunosorbent assay(ELISA) technique.RESULTS: hs CRP [0.45(0.07-2.63) mg/L vs 0.25(0.03-1.2) mg/L, P =0.02)] and Fetuin-A levels(50.27 ±5.04 vs44.99±10.28 ng/m L, P =0.009) were higher in the patients than in the control groups. We could not find significant difference in MGP level between two groups(P =0.08).There was not a significant correlation between MGP with Fetuin-A and hs CRP among the patients(P =0.7, P =0.9respectively). A significant negative correlation of hs CRP with Fetuin-A was observed in both case and control groups(P =0.004, r =-0.33 and P =0.001, r =-0.54,respectively).CONCLUSION: Although our study shows that serum hs CRP and Fetuin-A is increased in CNV patients as well as negatively correlated with both study groups, their direct role on pathogenesis of ARMD required future studies.展开更多
基金the Key Projects of Equipment Pre-research Foundation of the Ministry of Equipment Development of the Central Military Commission of China (No.6140922010201)the Key R&D Plan of Zhenjiang in 2018(No.GY2018021)。
文摘The performance of solid solution aging treatment on aluminum matrix composites prepared by powder metallurgy and reinforced with 6061 aluminum alloy powder as matrix;meanwhile, nano silicon carbide particles(nm Si Cp), submicron silicon carbide particles(1 μm Si Cp) and Ti particles were studied. The Al/Si Cp composite powder was prepared by high-energy ball milling, and then cold-pressed, sintered, hotextruded, and then heat-treated with different solution temperatures and aging times for the extruded composites. Optical microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy(EDS), X-ray diffractometer(XRD) and extrusion testing were used to analyze and test the microstructure and mechanical properties of aluminum matrix composites. The results show that after the multi-stage solid solution at 530 ℃×2 h+535 ℃×2 h+540 ℃×2 h, the particles are mainly equiaxed grains and uniformly distributed. There is no reinforcement agglomeration, and the surface is dense and the insoluble phase is basically dissolved. In the matrix, the strengthening effect is good, and the hardness and compressive strength are 179.43 HV and 680.42 MPa, respectively. Under this solution process, when the aluminum matrix composites are aged at 170 ℃ for 10 h, the hardness and compressive strength can reach their peaks and increase to 195.82 HV and 721.48 MPa, respectively.
基金Bengt Ihre Foundation,No.SLS-594-301 and SLS-789091
文摘Serum levels of cartilage oligomeric matrix protein(COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has also been associated with hepatocellular carcinoma. We present a prospective analysis of this biomarker in38 patients with chronic hepatitis C who were subject to eradication therapy with direct acting antivirals. We confirm previous studies associating COMP elevation with liver cirrhosis. We also show how viral levels are correlated with COMP at baseline. In our prospective analysis, we report that successful eradication of hepatitis C results in improvement in liver stiffness and laboratory liver function tests at 1 year follow-up. In contrast, median COMP-levels remain unchanged during the study period. We conclude that the biomarker potential of COMP in the prospective evaluation of liver diseases, remains to be elucidated.
基金the National Council for Science and Technology,No.SALUD-2016-272579 and No.PAPIIT-UNAM TA200515.
文摘BACKGROUND Matrix metalloproteinases(MMPs)participate in the degradation of extracellular matrix compounds,maintaining the homeostasis between fibrogenesis and fibrolytic processes in the liver.However,there are few studies on the regulation of liver MMPs in fibrosis progression in humans.AIM To assess the production activity and regulation of matrix metalloproteinases in liver fibrosis stages in chronic hepatitis C(CHC).METHODS A prospective,cross-sectional,multicenter study was conducted.CHC patients were categorized in fibrosis grades through FibroTest®and/or FibroScan®.Serum MMP-2,-7,and-9 were determined by western blot and multiplex suspension array assays.Differences were validated by the Kruskal-Wallis and Mann-Whitney U tests.The Spearman correlation coefficient and area under the receiver operating characteristic curve were calculated.Collagenolytic and gelatinase activity was determined through the Azocoll substrate and zymogram test,whereas tissue inhibitor of metalloproteinase-1 production was determined by dot blot assays.RESULTS Serum concentrations of the MMPs evaluated were higher in CHC patients than in healthy subjects.MMP-7 distinguished early and advanced stages,with a correlation of 0.32(P<0.001),and the area under the receiver operating characteristic displayed moderate sensitivity and specificity for MMP-7 in F4(area under the receiver operating characteristic,0.705;95%confidence interval:0.605-0.805;P<0.001).Collagenolytic activity was detected at F0 and F1,whereas gelatinase activity was not detected at any fibrosis stage.Tissue inhibitor of metalloproteinase-1 determination showed upregulation in F0 and F1 but downregulation in F2(P<0.001).CONCLUSION High concentrations of inactive MMPs were present in the serum of CHC patients,reflecting the impossibility to restrain liver fibrosis progression.MMPs could be good diagnostic candidates and therapeutic targets for improving novel strategies to reverse liver fibrosis in CHC.
基金Funded by the National Natural Science Foundation of China (50873047)the Natural Science Foundation of Gansu Province (3ZS061-A25-039)
文摘In order to improve wear resistance and decrease the cost, carbon and carbon nanotubes reinforced copper matrix composites were fabricated by the power metallurgy method. The effects of carbon （carbon and carbon nanotubes） volume fraction and applied load on the friction coefficient and wear rate under dry sliding of the composites were investigated at room temperature. By scanning electron microscopy （SEM）, the worn surfaces and debris were observed, and wear mechanism was also analyzed and discussed. The experimental wear process consists of the run-in, steady wear and severe wear process with the increasing of sliding distance. Both the friction coefficient and wear rate of the composites first decrease and then increase with the increasing of carbon volume fraction. The minimum friction coefficient and wear rate are obtained when carbon is 4.0vo1%. The wear mechanisms of the composites change from the adhesive wear and delamination wear to abrasive wear with the increasing of carbon volume fraction.
文摘We derive necessary and sufficient conditions for the existence of a Hermitian nonnegative-definite solution to the matrix equation AXB = C. Moreover, we derive a representation of a general Hermitian nonnegative-definite solution. We then apply our solution to two examples, including a comparison of our solution to a proposed solution by Zhang in [1] using an example problem given from [1]. Our solution demonstrates that the proposed general solution from Zhang in [1] is incorrect. We also give a second example in which we derive the general covariance structure so that two matrix quadratic forms are independent.
基金supported by the Ontario Research and Development Challenge Fund (ORDCF)GenSci Regeneration Sciences Inc. (Toronto,Canada)
文摘Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, and bone sialoprotein （BSP） were detected by immunohistochemistry in C2C12 cultured from day 1 to day 28. Results The signaling of bone matrix protein expression became weaker except for type I collagen, OC and BSP after 5 days. Fourteen days after culture, the positive signaling of type I collagen, OPN, ON, OC, and BSP was gradually declined, and could be detected significantly as compared with that of the negative control on day 28. BMP assay showed that the lkaline phosphatase （ALP） activity was higher in C2C12 culture than in the control during the 14-day culture. Also, total protein and DNA significantly increased during the 14-day culture. High levels of ALP were seen in preosteoblasts and osteoblsts in vivo and in differentiating osteoblasts in vitro. ALP was well recognized as a marker reflecting osteoblastic activity. Conclusion Native bovine BMP induces conversion of myoblasts into osteoblasts, produces type I collagen, and plays significantly role in osteoinduction and bone matrix mineralization of C2C 12 in vitro.
基金Funded by the National Natural Science Foundation of China(Nos.51275490,and 51475346)Specialized Research Fund for the Doctoral Program of Higher Education of China(20131420120002)Shanxi Province Science Foundation(2013011025-1)
文摘The selection of milling tools for Si C14Cu4Mg0.5Si based on Aluminium matrix 2A14 was analyzed, and the factors that affect the efficiency of the milling were discussed. The Si C14Cu4Mg0.5Si was designed for use on the moon landing vehicle or missile wings, but the hardness of aluminium-silicon carbide composite material was very high, much higher than the general hardness of cemented carbide, which will bring many difficulties in the aluminium-silicon carbide composite material processing. The chemical compositions of Si C14Cu4Mg0.5Si were analyzed. A new selected indexable cutter was designed to mill Si C14Cu4Mg0.5Si. The structure design of milling cutter was different from the conventional milling cutter, breaking the previous limitations to a certain extent, pioneering the idea. The tool material wear was detected by experiments. The mechanical and physical properties of Si C14Cu4Mg0.5Si were also tested. Si C14Cu4Mg0.5Si exhibited different surface quality characteristics under different milling tools.
文摘Cr3 C2-NiCr particles were injected into the melted surface of Q235 low carbon steel to make a surface metal matrix composite （MMC） layer by gas tungsten are melt injection （GTAMI） process. Hardness of the surface MMC layer was tested. Wear resistance of the surface MMC was investigated with a ball-on-disk dry sliding setup. Microstrnetures of the surface MMC layer and morphology of the worn surfaces were investigated with scanning electron microscopy （SEM）. The results showed that the hardness of the MMC was as high as 1 960. 7 HV. Wear loss of the upper part of the MMC layer is onlyO. 8% of that of the substrate under the dry sliding condition given. Wear loss of the bottom part is 2. 5 % of that of the substrate.
基金Project supported by the National Key Research and Development Program of China(Grant No.2017YFA0402300)the National Natural Science Foundation of China(Grant Nos.U1530142,11474032,and 11774344)the Young Teachers Scientific Research Ability Promotion Plan of Northwest Normal University(Grant No.NWNU-LKQN-15-3)
文摘The photoionization cross section of the ground state 2s22p 2Po1/2 and the first excited state 2s22p2po1/2 of C II ions are systematically calculated using the fully relativistic R-matrix code DARC. The detailed resonances are presented and identified for the photon energy ranging from threshold （24.38 eV） up to 41.5 eV where the L-shell （2p, 2s） photoionization process is dominant. In the calculations, the relativistic effect and electronic correlation effect are well considered. It is found that the relativistic effect is very important for the light atomic system CII, which accounts for experimentally observed fine structure resonance peaks. A careful comparison is made between the present results and the experimental values, and also other theoretical data available in the literature, showing that good agreement is obtained for the resonance peaks.
文摘Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. Contrary to normal cells, proteins of this group are preferentially phosphorylated. Phosphoproteins of hepatoma nuclear matrix are selectively subjected to rapid proteolysis. By alkali treatment and a monoclonal antibody against phosphotyrosyl residue the presence of two high molecular weight bands of phosphotyrosyl-containing proteins was detected in nuclear matrices of tumor but not of normal liver cells. High molecular weight protein group of tumor nuclear matrices revealed also a rapid turnover and preferential incorporation of labeled amino acids selectively inhibited by chloramphenicol.
文摘Objective： Nuclear matrix protein is tissue, cell-type specific, and tumor-relative. It plays an important role in the regulation of intranuclear processes. Some researches also showed that a c-erbB-2 promoter-specific DNA-binding nuclear matrix protein is present only in malignant human breast tissues and induces mitogenesis and cell surface expression of the c-erbB-2 protein in resting NIH/3T3 cells. But it is not clear that how it in colon adenocarcinomas. Methods： Two-dimensional gel electrophoretic method was used for NMP identification and immunohistochemistry was used for c-erbB-2 detection in 12 cases of colon adenocarcinomas and matched adjacent normal colon tissues. Results： 5 different nuclear matrix proteins （named C1-C5） were identified in 12 colon adenocarcinoma specimens, but not in the matched adjacent normal colon tissues; 3 nuclear matrix proteins （named N1-N3） were identified in all 12 matched adjacent normal colon tissues, but not in colon adenocarcinoma specimens. A nuclear matrix protein （named N4） was detected in all of 9 moderated-well differentiated adenocarcinomas and all 12 matched adjacent normal colon tissues, but not in 3 poor-differentiated adenocarcinomas. All of the 10 colon adenocarcinomas which had the nuclear matrix protein C4 were c-erbB-2 expression positive. Conclusion： The data suggest that there are specific nuclear matrix proteins in colon adenocarcinomas and its subtypes, which maybe valuable to serve as markers of colon adenocarcinomas in future. Nuclear matrix protein C4 probably is a c-erbB-2 promotor-specific nuclear matrix protein in colon adenocarcinomas, and may induce the expression of c-erbB-2.
基金Supported by the vice-chancellor for research of Tabriz University of Medical Sciences, Tabriz, Iran
文摘AIM: To evaluate the high sensitivity C-reactive protein(hs CRP), Fetuin-A and matrix γ-carboxyglutamate protein(MGP) as the main factors for vascular calcification and inflammation in serum of patients with advanced age-related macular degeneration(ARMD) in comparison to healthy controls. METHODS: The subjects were 40 patients with choroidal neovascularization(CNV) having a mean age of70.9 ±9.1y and a matched group of 49 apparently healthy control subjects. The ARMD was diagnosed using a slitlamp with superfield lens, fundus photography and fluorescein angiography. Measurement of hs CRP was done by nephelometry method. Levels of Fetuin-A and MGP were measured by enzyme-linked immunosorbent assay(ELISA) technique.RESULTS: hs CRP [0.45(0.07-2.63) mg/L vs 0.25(0.03-1.2) mg/L, P =0.02)] and Fetuin-A levels(50.27 ±5.04 vs44.99±10.28 ng/m L, P =0.009) were higher in the patients than in the control groups. We could not find significant difference in MGP level between two groups(P =0.08).There was not a significant correlation between MGP with Fetuin-A and hs CRP among the patients(P =0.7, P =0.9respectively). A significant negative correlation of hs CRP with Fetuin-A was observed in both case and control groups(P =0.004, r =-0.33 and P =0.001, r =-0.54,respectively).CONCLUSION: Although our study shows that serum hs CRP and Fetuin-A is increased in CNV patients as well as negatively correlated with both study groups, their direct role on pathogenesis of ARMD required future studies.
