Expression of insecticidal protein for transgenic Bacillus thuringiensis （Bt） cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbo...Expression of insecticidal protein for transgenic Bacillus thuringiensis （Bt） cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio （C/N） and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha-l, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of inseetiocidal efficacy in Bt cotton, especially in peak boll period.展开更多
Soil carbon to nitrogen(C/N) ratio is one of the most important variables reflecting soil quality and ecological function,and an indicator for assessing carbon and nitrogen nutrition balance of soils.Its variation ref...Soil carbon to nitrogen(C/N) ratio is one of the most important variables reflecting soil quality and ecological function,and an indicator for assessing carbon and nitrogen nutrition balance of soils.Its variation reflects the carbon and nitrogen cycling of soils.In order to explore the spatial variability of soil C/N ratio and its controlling factors of the Ili River valley in Xinjiang Uygur Autonomous Region,Northwest China,the traditional statistical methods,including correlation analysis,geostatistic alanalys and multiple regression analysis were used.The statistical results showed that the soil C/N ratio varied from 7.00 to 23.11,with a mean value of 10.92,and the coefficient of variation was 31.3%.Correlation analysis showed that longitude,altitude,precipitation,soil water,organic carbon,and total nitrogen were positively correlated with the soil C/N ratio(P < 0.01),whereas negative correlations were found between the soil C/N ratio and latitude,temperature,soil bulk density and soil p H.Ordinary Cokriging interpolation showed that r and ME were 0.73 and 0.57,respectively,indicating that the prediction accuracy was high.The spatial autocorrelation of the soil C/N ratio was 6.4 km,and the nugget effect of the soil C/N ratio was 10% with a patchy distribution,in which the area with high value(12.00–20.41) accounted for 22.6% of the total area.Land uses changed the soil C/N ratio with the order of cultivated land > grass land > forest land > garden.Multiple regression analysis showed that geographical and climatic factors,and soil physical and chemical properties could independently explain 26.8%and 55.4% of the spatial features of soil C/N ratio,while human activities could independently explain 5.4% of the spatial features only.The spatial distribution of soil C/N ratio in the study has important reference value for managing soil carbon and nitrogen,and for improving ecological function to similar regions.展开更多
Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces...Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.展开更多
Soil organic carbon and nitrogen are used as indexes of soil quality assessment and sustainable land use management. At the same time, soil C/N ratio is a sensitive indicator of soil quality and for assessing the carb...Soil organic carbon and nitrogen are used as indexes of soil quality assessment and sustainable land use management. At the same time, soil C/N ratio is a sensitive indicator of soil quality and for assessing the carbon and nitrogen nutrition balance of soils. We studied the characteristics of soil organic carbon and total nitrogen by investigating a large number of apple orchards in major apple production areas in China. High apple orchard soil organic carbon content was observed in the provinces of Heilongjiang, Xinjiang, and Yunnan, whereas low content was found in the provinces of Shandong, Henan, Hebei, and Shaanxi, with the values ranging between 6.44 and 7.76 g·kg-1. Similar to soil organic carbon, soil total nitrogen content also exhibited obvious differences in the 12 major apple producing provinces. Shandong apple orchard soil had the highest total nitrogen content (1.26 g·kg-1), followed by Beijing (1.23 g·kg-1). No significant difference was noted between these two regions, but their total nitrogen content was significantly higher than the other nine provinces, excluding Yunnan. The soil total nitrogen content for Xinjiang, Heilongjiang, Hebei, Henan, and Gansu was between 0.87 and 1.03 g·kg-1, which was significantly lower than that in Shandong and Beijing, but significantly higher than that in Liaoning, Shanxi, and Shaanxi. Six provinces exhibited apple orchard soil C/N ratio higher than 10, including Heilongjiang (15.42), Xinjiang (13.38), Ningxia (14.45), Liaoning (12.24), Yunnan (11.03), and Gansu (10.63). The soil C/N ratio was below 10 in the remaining six provinces, in which the highest was found in Shaanxi (9.47), followed by Beijing (8.98), Henan (7.99), and Shanxi (7.62), and the lowest was found in Hebei (6.80) and Shandong (6.05). Therefore, the improvement of soil organic carbon should be given more attention to increase the steady growth of soil C/N ratio.展开更多
On the basis of multi-proxy analysis on TOC, TN, C/N, organic δ13C and grain size, sedi- ment record from Qinghai Lake provides evidences of stepwise-patterned climatic change since 16 ka BP. Results show that Qingha...On the basis of multi-proxy analysis on TOC, TN, C/N, organic δ13C and grain size, sedi- ment record from Qinghai Lake provides evidences of stepwise-patterned climatic change since 16 ka BP. Results show that Qinghai Lake underwent six environmental stages. From 16.2 to 14.3 ka BP and from 4.0 to 2.1 ka BP, the organic δ13C value was controlled by the concentration of atmospheric CO2. Relative higher organic δ13C values occurred between 14.3 to 10.4 ka BP indicative of water hardness decrease resulted from melting ice water, corresponding to two intervals of C/N peak values to the B?ling and Al- ler?d warm periods in Europe respectively. From 10.4 ka BP, Qinghai Lake entered the Holocene and the climate was warm and a little dry. The Megathermal appeared at about 6.7 ka BP when the vegetation around the lake transformed into a forest. Between 6.3 ka BP and 4.0 ka BP, the temperature decreased and δ13C value was controlled by the expansion of C3 plants and the retreat of C4 plants in river catchment. Since 4.0 ka BP, the climate gradually became cold and dry. From 2.1 ka BP, the cold-dry climate and human activity resulted in an abrupt increase in C/N with deceased δ13C value; meanwhile, many coarse grains appeared in sediments.展开更多
Elemental analyzer/isotope ratio mass spectrometry(EA/TRMS) has been widely applied to analyze the^(15)N/^(14)N isotope composition(δ^(15)N) of plants and soils,but the δ^(15)N results may be inaccurate due to incom...Elemental analyzer/isotope ratio mass spectrometry(EA/TRMS) has been widely applied to analyze the^(15)N/^(14)N isotope composition(δ^(15)N) of plants and soils,but the δ^(15)N results may be inaccurate due to incomplete combustion of the high-C/N-ratio plant samples by EA.Therefore,it is necessary to develop a method to solve the problem of imperfect combustion.In this study,we used two methods:1) adding copper oxide powder to the samples,and 2) increasing the O_2 flow(from 100 mL min^(-1) to 200 mL min^(-1)) for the auto sampler inlet purge line of the EA.The δ^(15)N values of the plant samples became more positive and tended to be stable after complete combustion.Also,the required blank samples for each plant sample decreased with increasing amount of the added CuO powder.However,at 200 mL min^(-1) of the oxygen flow in the EA,complete combustion could not be achieved without adding copper oxide,but this was done with decreased amount of CuO powder.Therefore,mixing cupric oxide into the high-C/N-ratio samples was an efficient,simple and convenient way to solve the problem of imperfect combustion in the EA.展开更多
In forage grasses, the nitrogen concentration is directly related to the nutritional value. The studies examined the hypothesis that global elevation of CO2 concentration probably affects the biomass, nitrogen （N） c...In forage grasses, the nitrogen concentration is directly related to the nutritional value. The studies examined the hypothesis that global elevation of CO2 concentration probably affects the biomass, nitrogen （N） concentration, and allocation and distribution patterns in the organs of forage grasses. While sainfoin （Onobrychis viciaefolia Scop.） seedlings grew on a low nutrient soil in closed chambers for 90 days, they were exposed to two CO2 concentrations （ambient or ambient＋350 μmol mol^-1 CO2） without adding nutrients to them. After 90 days exposure to CO2, the biomasses of leaves, stems, and roots, and N concentrations and contents of different parts were measured. Compared with the ambient CO2 concentration, the elevated CO2 concentration increased the total dry matter by 25.07%, mainly due to the root and leaf having positive response to the elevated CO2 concentration. However, the elevated CO2 concentration did not change the proportions of the dry matters in different parts and the total plants compared with the ambient CO2 concentration. The elevated CO2 concentration lowered the N concentrations of the plant parts. Because the dry matter was higher, the elevated CO2 concentration had no effect on the N content in the plants compared to the ambient CO2 concentration. The elevated CO2 concentration promoted N allocations of the different parts significantly and increased N allocation of the underground part. The results have confirmed the previous suggestions that the elevated CO2 concentration stimulates plant biomass production and decreases the N concentrations of the plant parts.展开更多
[ Objective] The research aimed to study influence of different C/N ratios on aerobic denitdfication characteristics of the strain. [ Method] 5 aerobic denitdfying bacteria were isolated from activated sludge at low t...[ Objective] The research aimed to study influence of different C/N ratios on aerobic denitdfication characteristics of the strain. [ Method] 5 aerobic denitdfying bacteria were isolated from activated sludge at low temperature and low C/N ratio, and their denitdfication capabilities were verified. Influence of different C/N ratios on denitdfication capability of the strain was determined. [ Result] In the five isolated aerobic denitdf- ying bacteria, except HFX08 was G-, HFX00, HFX01, HFX12 and HFX13 were G ＋. HFX08 belonged to Pseudomonas, and other strains be- longed to Acinetobacter. As C/N ratio increased, denitdfication velocity increased, and the highest removal rate of nitrogen for the five strains could reach over 92%. [ Conclusion] The research could provide reference for design and operation of the sewage treatment process with low C/N ratio in winter in the north.展开更多
基金supported by the National Natural Science Foundation of China(31171479)the Key Laboratory Foundation of Jiangsu Province,China(10KJA210057)+2 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions,China,the Doctoral Advisor Foundation of Education Department of China(20113250110001)the Project of National Agricultural Modern Industrial Technology System Post Experts,China(CARS-18-20)the Graduate Student Scientific Research Innovation Projects of Jiangsu Province,China(CXLX11_1019)
文摘Expression of insecticidal protein for transgenic Bacillus thuringiensis （Bt） cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio （C/N） and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha-l, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of inseetiocidal efficacy in Bt cotton, especially in peak boll period.
基金Under the auspices of National Science and Technology Support Program of China(No.2014BAC15B03)the West Light Funds of Chinese Academy of Sciences(No.YB201302)
文摘Soil carbon to nitrogen(C/N) ratio is one of the most important variables reflecting soil quality and ecological function,and an indicator for assessing carbon and nitrogen nutrition balance of soils.Its variation reflects the carbon and nitrogen cycling of soils.In order to explore the spatial variability of soil C/N ratio and its controlling factors of the Ili River valley in Xinjiang Uygur Autonomous Region,Northwest China,the traditional statistical methods,including correlation analysis,geostatistic alanalys and multiple regression analysis were used.The statistical results showed that the soil C/N ratio varied from 7.00 to 23.11,with a mean value of 10.92,and the coefficient of variation was 31.3%.Correlation analysis showed that longitude,altitude,precipitation,soil water,organic carbon,and total nitrogen were positively correlated with the soil C/N ratio(P < 0.01),whereas negative correlations were found between the soil C/N ratio and latitude,temperature,soil bulk density and soil p H.Ordinary Cokriging interpolation showed that r and ME were 0.73 and 0.57,respectively,indicating that the prediction accuracy was high.The spatial autocorrelation of the soil C/N ratio was 6.4 km,and the nugget effect of the soil C/N ratio was 10% with a patchy distribution,in which the area with high value(12.00–20.41) accounted for 22.6% of the total area.Land uses changed the soil C/N ratio with the order of cultivated land > grass land > forest land > garden.Multiple regression analysis showed that geographical and climatic factors,and soil physical and chemical properties could independently explain 26.8%and 55.4% of the spatial features of soil C/N ratio,while human activities could independently explain 5.4% of the spatial features only.The spatial distribution of soil C/N ratio in the study has important reference value for managing soil carbon and nitrogen,and for improving ecological function to similar regions.
基金Supported by the Yangtze Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT_17R81)the Technology Support Project of Tianjin(No.16YFZCNC00810)
文摘Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.
文摘Soil organic carbon and nitrogen are used as indexes of soil quality assessment and sustainable land use management. At the same time, soil C/N ratio is a sensitive indicator of soil quality and for assessing the carbon and nitrogen nutrition balance of soils. We studied the characteristics of soil organic carbon and total nitrogen by investigating a large number of apple orchards in major apple production areas in China. High apple orchard soil organic carbon content was observed in the provinces of Heilongjiang, Xinjiang, and Yunnan, whereas low content was found in the provinces of Shandong, Henan, Hebei, and Shaanxi, with the values ranging between 6.44 and 7.76 g·kg-1. Similar to soil organic carbon, soil total nitrogen content also exhibited obvious differences in the 12 major apple producing provinces. Shandong apple orchard soil had the highest total nitrogen content (1.26 g·kg-1), followed by Beijing (1.23 g·kg-1). No significant difference was noted between these two regions, but their total nitrogen content was significantly higher than the other nine provinces, excluding Yunnan. The soil total nitrogen content for Xinjiang, Heilongjiang, Hebei, Henan, and Gansu was between 0.87 and 1.03 g·kg-1, which was significantly lower than that in Shandong and Beijing, but significantly higher than that in Liaoning, Shanxi, and Shaanxi. Six provinces exhibited apple orchard soil C/N ratio higher than 10, including Heilongjiang (15.42), Xinjiang (13.38), Ningxia (14.45), Liaoning (12.24), Yunnan (11.03), and Gansu (10.63). The soil C/N ratio was below 10 in the remaining six provinces, in which the highest was found in Shaanxi (9.47), followed by Beijing (8.98), Henan (7.99), and Shanxi (7.62), and the lowest was found in Hebei (6.80) and Shandong (6.05). Therefore, the improvement of soil organic carbon should be given more attention to increase the steady growth of soil C/N ratio.
基金Supported by National Key Basic Research Fund (No. 2004CB720205) and National Nature and Science Foundation of China (No. 40331003).
文摘On the basis of multi-proxy analysis on TOC, TN, C/N, organic δ13C and grain size, sedi- ment record from Qinghai Lake provides evidences of stepwise-patterned climatic change since 16 ka BP. Results show that Qinghai Lake underwent six environmental stages. From 16.2 to 14.3 ka BP and from 4.0 to 2.1 ka BP, the organic δ13C value was controlled by the concentration of atmospheric CO2. Relative higher organic δ13C values occurred between 14.3 to 10.4 ka BP indicative of water hardness decrease resulted from melting ice water, corresponding to two intervals of C/N peak values to the B?ling and Al- ler?d warm periods in Europe respectively. From 10.4 ka BP, Qinghai Lake entered the Holocene and the climate was warm and a little dry. The Megathermal appeared at about 6.7 ka BP when the vegetation around the lake transformed into a forest. Between 6.3 ka BP and 4.0 ka BP, the temperature decreased and δ13C value was controlled by the expansion of C3 plants and the retreat of C4 plants in river catchment. Since 4.0 ka BP, the climate gradually became cold and dry. From 2.1 ka BP, the cold-dry climate and human activity resulted in an abrupt increase in C/N with deceased δ13C value; meanwhile, many coarse grains appeared in sediments.
文摘Elemental analyzer/isotope ratio mass spectrometry(EA/TRMS) has been widely applied to analyze the^(15)N/^(14)N isotope composition(δ^(15)N) of plants and soils,but the δ^(15)N results may be inaccurate due to incomplete combustion of the high-C/N-ratio plant samples by EA.Therefore,it is necessary to develop a method to solve the problem of imperfect combustion.In this study,we used two methods:1) adding copper oxide powder to the samples,and 2) increasing the O_2 flow(from 100 mL min^(-1) to 200 mL min^(-1)) for the auto sampler inlet purge line of the EA.The δ^(15)N values of the plant samples became more positive and tended to be stable after complete combustion.Also,the required blank samples for each plant sample decreased with increasing amount of the added CuO powder.However,at 200 mL min^(-1) of the oxygen flow in the EA,complete combustion could not be achieved without adding copper oxide,but this was done with decreased amount of CuO powder.Therefore,mixing cupric oxide into the high-C/N-ratio samples was an efficient,simple and convenient way to solve the problem of imperfect combustion in the EA.
基金supported by the Specialized Research Fund for the Doctoral Program of Higher Education(SRFDP200807181008)the Science Fund of Shaanxi Normal University for the Young Scholars,China
文摘In forage grasses, the nitrogen concentration is directly related to the nutritional value. The studies examined the hypothesis that global elevation of CO2 concentration probably affects the biomass, nitrogen （N） concentration, and allocation and distribution patterns in the organs of forage grasses. While sainfoin （Onobrychis viciaefolia Scop.） seedlings grew on a low nutrient soil in closed chambers for 90 days, they were exposed to two CO2 concentrations （ambient or ambient＋350 μmol mol^-1 CO2） without adding nutrients to them. After 90 days exposure to CO2, the biomasses of leaves, stems, and roots, and N concentrations and contents of different parts were measured. Compared with the ambient CO2 concentration, the elevated CO2 concentration increased the total dry matter by 25.07%, mainly due to the root and leaf having positive response to the elevated CO2 concentration. However, the elevated CO2 concentration did not change the proportions of the dry matters in different parts and the total plants compared with the ambient CO2 concentration. The elevated CO2 concentration lowered the N concentrations of the plant parts. Because the dry matter was higher, the elevated CO2 concentration had no effect on the N content in the plants compared to the ambient CO2 concentration. The elevated CO2 concentration promoted N allocations of the different parts significantly and increased N allocation of the underground part. The results have confirmed the previous suggestions that the elevated CO2 concentration stimulates plant biomass production and decreases the N concentrations of the plant parts.
基金Special Funds for Environmental Protection of the Governmental Public Industry Research,China(200909043)
文摘[ Objective] The research aimed to study influence of different C/N ratios on aerobic denitdfication characteristics of the strain. [ Method] 5 aerobic denitdfying bacteria were isolated from activated sludge at low temperature and low C/N ratio, and their denitdfication capabilities were verified. Influence of different C/N ratios on denitdfication capability of the strain was determined. [ Result] In the five isolated aerobic denitdf- ying bacteria, except HFX08 was G-, HFX00, HFX01, HFX12 and HFX13 were G ＋. HFX08 belonged to Pseudomonas, and other strains be- longed to Acinetobacter. As C/N ratio increased, denitdfication velocity increased, and the highest removal rate of nitrogen for the five strains could reach over 92%. [ Conclusion] The research could provide reference for design and operation of the sewage treatment process with low C/N ratio in winter in the north.
