Objective This study aimed to investigate the regulation of histone-like nucleoid structuring protein(H-NS)on biofilm formation and cyclic diguanylate(c-di-GMP)synthesis in Vibrio parahaemolyticus RIMD2210633.Methods ...Objective This study aimed to investigate the regulation of histone-like nucleoid structuring protein(H-NS)on biofilm formation and cyclic diguanylate(c-di-GMP)synthesis in Vibrio parahaemolyticus RIMD2210633.Methods Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining,quantification of c-di-GMP,quantitative real-time polymerase chain reaction,LacZ fusion,and electrophoretic-mobility shift assay.Results The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V.parahaemolyticus RIMD2210633.H-NS can bind the upstream promoter-proximal DNA regions of scrA,scrG,VP0117,VPA0198,VPA1176,VP0699,and VP2979 to repress their transcription.These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism.Conclusion One of the mechanisms by which H-NS represses the biofilm formation by V.parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP.展开更多
Nucleotide second messengers are highly versatile signaling molecules that regulate a variety of key biological processes in bacteria.The best-studied examples are cyclic AMP(cAMP)and bis-(3'-5')-cyclic dimeri...Nucleotide second messengers are highly versatile signaling molecules that regulate a variety of key biological processes in bacteria.The best-studied examples are cyclic AMP(cAMP)and bis-(3'-5')-cyclic dimeric guanosine monophosphate(c-di-GMP),which both act as global regulators.Global regulatory frameworks of c-di-GMP and cAMP in bacteria show several parallels but also significant variances.In this review,we llustrate the global regulatory models of the two nucleotide second messengers,compare the different regulatory frameworks between c-di-GMP and cAMP,and discuss the mechanisms and physiological significance of cross-regulation between c-di-GMP and cAMP.c-di-GMP responds to numerous signals de-pendent on a great number of metabolic enzymes,and it regulates various signal transduction pathways through its huge number of effectors with varying activities.In contrast,due to the limited quantity,the cAMP metabolic enzymes and its major effector are regulated at different levels by diverse signals.cAMP performs its global regulatory function primarily by controlling the transcription of a large number of genes via cAMP receptor protein(CRP)in most bacteria.This review can help us understand how bacteria use the two typical nucleotide second messengers to effectively coordinate and integrate various physiological processes,providing theoretical guidelines for future research.展开更多
基金supported by grants from the National Natural Science Foundation of China[Grant No.82072239]。
文摘Objective This study aimed to investigate the regulation of histone-like nucleoid structuring protein(H-NS)on biofilm formation and cyclic diguanylate(c-di-GMP)synthesis in Vibrio parahaemolyticus RIMD2210633.Methods Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining,quantification of c-di-GMP,quantitative real-time polymerase chain reaction,LacZ fusion,and electrophoretic-mobility shift assay.Results The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V.parahaemolyticus RIMD2210633.H-NS can bind the upstream promoter-proximal DNA regions of scrA,scrG,VP0117,VPA0198,VPA1176,VP0699,and VP2979 to repress their transcription.These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism.Conclusion One of the mechanisms by which H-NS represses the biofilm formation by V.parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP.
基金funded by the National Natural Science Foundation of China(Nos.32370023,32370583,31970036,and 31900401)Natural Science Foundation of the Jiangsu Higher Education Institutions of China(20KJB180001 and 20KJA180007)+2 种基金Natural Science Foundation of Jiangsu Province(BK20210920 and BK20231350)Jiangsu Agricultural Science and Technology Innovation Fund(Cx223125)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Nucleotide second messengers are highly versatile signaling molecules that regulate a variety of key biological processes in bacteria.The best-studied examples are cyclic AMP(cAMP)and bis-(3'-5')-cyclic dimeric guanosine monophosphate(c-di-GMP),which both act as global regulators.Global regulatory frameworks of c-di-GMP and cAMP in bacteria show several parallels but also significant variances.In this review,we llustrate the global regulatory models of the two nucleotide second messengers,compare the different regulatory frameworks between c-di-GMP and cAMP,and discuss the mechanisms and physiological significance of cross-regulation between c-di-GMP and cAMP.c-di-GMP responds to numerous signals de-pendent on a great number of metabolic enzymes,and it regulates various signal transduction pathways through its huge number of effectors with varying activities.In contrast,due to the limited quantity,the cAMP metabolic enzymes and its major effector are regulated at different levels by diverse signals.cAMP performs its global regulatory function primarily by controlling the transcription of a large number of genes via cAMP receptor protein(CRP)in most bacteria.This review can help us understand how bacteria use the two typical nucleotide second messengers to effectively coordinate and integrate various physiological processes,providing theoretical guidelines for future research.
