The role of C-type natriuretic peptide in rat testes during spermatogenesis

C-type natriuretic peptide （CNP） is a 22-amino acid peptide and act as a local paracrine or autocrine regulator. There is growing evidence that ChIP is involved in male reproductive processes. To investigate the role of CNPduring spermatogenesis, we measured the mRNA expression of CNPand its specific membrane-bound natriuretic peptide receptor-B （NPR-B） using real-time RT-PCR in the testes of normal rats on different postnatal days. After that spermatogenesis dysfunction model induced by ornidazole was established with the aim to study the correlation of CNPwith spermatogenic dysfunction. Then, Sertoli cells from 18- to 22-day-old healthy male rats were cultured in the presence of different CNPconcentrations （1 ×10-6, 1×10-7 and1×10-8 mol l-1）, and the mRNA expression levels of androgen.binding protein, inhibin B and transferrin were examined at 0 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 48 h. During the postnatal development of rat testes, the highest mRNA expression levels of CNPand NPR-B were found at postnatal Do, and the levels then declined gradually, with a second ChIPpeak at postnatal D35. In the ornidazole-induced infertile rat testes, CIVPgene expression was lower than in the uninduced rats （P〈0.05）, while IVPR-Bgene expression was greater （P〈0.05）. In cultured Sertoli cells, supplementation with CNP stimulated the gene expression of androgen-binding pmteirginhibin B/transferrin, particularly at 12 h, and 1× 10-7 mol l-1 CNP had the highest upregulation effect. The gene expression levels of CNPIIVPR-B in rat testes at different postnatal stages and in infertile rat testes indicated that CNP may participate in the physiology and/or pathology related to spermatogenesis. Moreover, ChIP regulated endocrine function in Sertoli cells. Taken together, these results showed that CNP is closely tied to spermatogenesis.

Forskolin Modulates the Inhibitory Effect of C-Type Natriuretic Peptide on Hypoxia-Induced Atrial Dynamics and Hypoxia Inducible Factor 1 Alpha Activity

Our study investigated effects of C-type natriuretic peptide (CNP) on atrial dynamics and hypoxia inducible factor 1 alpha (HIF-1α) activity in perfused beating rat atria, under hypoxic conditions. Hypoxia significantly increased the levels of HIF-1α, concomitant with decreased trial dynamics. CNP (0.1 μmol/L) further decreased atrial dynamics under hypoxia and suppressed hypoxia-induced stimulation of HIF-1α expression. An adenylylcyclase (AC) activator, forskolin (0.1 μmol/L), significantly up-regulated atrial phosphodiesterase subtype 3A (PDE 3A) protein without affecting hypoxia-induced dynamics. In the presence of forskolin, the inhibitory effects of CNP on hypoxia-induced atrial dynamics and HIF-1α levels were significantly attenuated. Forskolin also prevented hypoxia-induced downregulation of PDE3A protein. These findings suggested that CNP inhibited atrial dynamics and HIF-1α activity in the isolated perfused beating rat atria under hypoxic conditions. Furthermore, both effects were modulated by the AC activator forskolin, through activation of CNP-PDE 3A signaling.

Role of C-type natriuretic peptide in the function of normal human sperm

C-type natriuretic peptide （CNP） is a newly discovered type of local regulatory factor that mediates its biological effects through the specific, membrane-bound natriuretic peptide receptor-B （NPR-B）. Recent studies have established that CNP is closely related to male reproductive function. The aims of this study were to determine the distribution of CNP/NPR-B in human ejaculated spermatozoa through different methods （such as immunolocalization, real time polymerase chain reaction and Western Blot）, and then to evaluate the influence of CNP on sperm function in vitro, such as motility and acrosome reaction. Human semen samples were collected from consenting donors who met the criteria of the World Health Organization for normozoospermia. Our results show that the specific receptor NPR-B of CNP is localized in the acrosomal region of the head and the membrane of the front-end tail of the sperm, and there is no signal of CNP in human sperm. Compared with the control, CNP can induce a significant dose-dependent increase in spermatozoa motility and acrosome reaction. In summary, CNP/NPR-B can affect sperm motility and acrosome reaction, thus regulating the reproductive function of males. CNP may be a new key factor in regulating sperm function.

Regulation of C-type Natriuretic Peptides and Natriuretic Peptide Receptor-B Expression in Diabetic Rats Renal Treated by Tongluo Recipe(通络方)

To investigate the expression of C-type natriuretic peptides （CNP） and natriuretic peptide receptor-B （NPR-B） receptor in diabetic rats renal cortex, and the regulation by Tongluo Recipe （通络方, TLR）. Methods： Sixty male SD rats were divided into 3 groups： the normal control group, diabetic model group and diabetic TLR group. Each group was further divided into two subgroups of ten in each, according to 4-week or 12-week observation period. Streptozotocin （STZ）-induced diabetic rats were treated with TLR （1.0 g.kgl＇d1） for 4 and 12 weeks, respectively. （1） The essential information was collected for comparing renal mass, serum creatinine and 24 h urine albumen on each group was calculated. （2） CNP mRNA and NPR-B mRNA were detected by realtime-polymerase chain reaction （PCR） on rats renal cortex. （3） Concentration of CNP on renal cortex or serum were analyzed by enzyme-linked immunosorbent assay （ELISA）. （4） Pathological evaluation and NPR-B immunostaining for renal tissue were also performed. Results： （1） CNP and NPR-B mRNA levels were detected in each treated or untreated group, with slight elevated in untreated diabetes rats administrated with STZ after 4-week and CNP mRNA level remarkable elevated at 39.21 times higher than normal control group after 12 weeks, but NPR-B mRNA level showed a remarkably down-regulation at 98.07% after 12 weeks. CNP mRNA of TLR-treated group was also elevated after 12-week treatment, but less than untreated group. （2） Concentrations of CNP in renal cortex were obviously increased in treated or untreated diabetes rats, within these groups the treatment of TLR was found more significantly on prompting CNP concentration. Comparing to normal group, serum concentrations of CNP were also increased in treated or untreated diabetic groups, but there was no difference between these diabetic groups. （3） Renal lesions like glomerular volume increased are observed mostly in the relative eady stage after 4 weeks. Although TLR treated group had no significant difference in their glomerular volume, the degrees of injury of glomerulus were ameliorated, as well as the NPR-B immunostaining enhanced in glomerulus. Weakly positive immunostaining of NPR-B are observed in glomerulus of normal control, and negative in glomerulus of untreated diabetes rats administrated with STZ after 12 weeks, whereas TLR-treatment groups showed a little enhancement. Conclusion： CNP and NPR-B showed different characteristic on renal cortex at different pathological period in diabetes rats, and TLR regulated their expression.

急性颅脑损伤患者血清NSE、IL-8和CNP水平的变化及临床意义

目的:探讨了急性颅脑损伤患者治疗前后血清NSE、IL-8和CNP水平的变化及临床意义。方法:应用放免法对30例急性颅脑损伤患者进行了血清NSE、IL-8和CNP的检测,并与35名正常健康人作比较。结果:急性颅脑损伤患者在治疗前血清NSE、IL-8水平非常显著地高于正常人组,而CNP水平又非常显著地低于正常人组(P<0.01)。经治疗2周后则与正常人组比较无显著性差异(P>0.05),且血清NSE水平与IL-8水平呈正相关(r=0.6188,P<0.01),与CNP水平呈显著负相关(r=-0.578,P<0.01)。结论:检测血清NSE、IL-8和CNP水平的变化有助于估计急性颅脑损伤患者病情的轻重及预后。

颅脑外伤患者血清CNP、IGF-Ⅱ含量与脑损伤、炎症反应的相关性研究

目的:研究颅脑外伤患者血清C型利钠多肽(CNP)和胰岛素样生长因子-Ⅱ(IGF-Ⅱ)含量与脑损伤、炎症反应的相关性。方法:将本院收治的颅脑外伤患者纳入研究的病例组、将同期体检的健康志愿者纳入对照组。测定血清中CNP、IGF-Ⅱ、神经标志分子、促炎细胞因子的含量以及外周血中炎症信号分子的表达量。结果:病例组血清中CNP、IGF-Ⅱ的含量均显著低于对照组,血清中UCH-L1、GFAP、S100B、Tau、MIP-1α、IL-1β、IL-6、IL-8、TNF-α的含量以及外周血中JAK2、STAT3、MEK、ERK1/2的mRNA表达量均显著高于对照组;病例组血清中CNP、IGF-Ⅱ的含量与血清中UCH-L1、GFAP、S100B、Tau、MIP-1α、IL-1β、IL-6、IL-8、TNF-α的含量以及外周血中JAK2、STAT3、MEK、ERK1/2的mRNA表达量呈负相关。结论:颅脑外伤患者血清CNP、IGF-Ⅱ含量的减少与脑损伤程度的加重、炎症反应的过度激活密切相关。

急性颅脑损伤患者血清NO、NOS和CNP测定的临床意义

目的:探讨了NO、NOS和血浆C-型利钠多肽(CNP)水平在急性颅脑损伤早期的病理、生理作用。方法:分别应用放免法测定血浆CNP水平,生化法测定NO、NOS水平。结果:在治疗前急性颅脑损伤患者血浆CNP水平和NO水平非常显著地低于正常人组水平(P<0.01),而NOS水平显著地高于正常人组水平(P<0.01),经治疗后一个月则与正常人组比较无显著性差异(P>0.05)。结论:观察血浆CNP、NO和NOS水平的变化对研究急性早期颅脑损伤的病理生理变化,判断疗效及预后观察是具有十分重要的临床意义。

COPD患者血清ANP、BNP和CNP测定及其临床意义

目的:探讨慢性阻塞性肺疾病(COPD)患者血清心钠素(ANP)、脑钠肽(BNP)、C型钠尿肽(CNP)水平的变化及其临床意义。方法:采用放射免疫分析79例COPD患者和36例健康对照组血清ANP、BNP和CNP水平,并进行统计分析。结果:COPD组血清ANP、BNP和CNP水平显著地高于健康对照组(t=3.6841,P<0.01;t=11.70,P<0.01;t=2.177,P<0.05),但Ⅰ、Ⅱ、Ⅲ和Ⅳ级组间血清ANP、BNP和CNP水平方差检验无显著性意义(F=2.123、F=1.515、F=0.165,P均>0.05)。相互间相关性分析揭示:ANP、BNP和CNP三者间均呈显著正相关(r=0.369,P<0.01;r=0.354,P<0.01;r=0.426,P<0.01)。住院期间死亡的患者血清ANP、BNP和CNP水平显著地高于好转出院的患者(t=5.149,P<0.01;t=4.875,P<0.01;t=2.830,P<0.01)。结论:COPD患者血清ANP、BNP和CNP显著升高,且与病人的稳定情况、肺动脉压力及预后相关。

CNP signal pathway up-regulated in rectum of depressed rats and the interventional effect of Xiaoyaosan

AIM:To investigate the distribution and expressionof C-type natriuretic peptide(CNP)/natriuretic peptide receptor B(NPR-B) in the rectum of a rodent depression model and the interventional effect of Xiaoyaosan(XYS).METHODS:Male rats(n = 45) of clean grade(200 ± 20 g) were divided into five groups after one week of adaptive feeding:primary control,depression model,low dose XYS,middle dose XYS,and high dose XYS.The animal experiment continued for 3 wk.Primary controls were fed normally ad libitum.The rats of all other groups were raised in solitary and exposed to classic chronic mild unpredictable stimulation each day.XYS groups were perfused intragastrically with low dose,middle dose,and high dose XYS one hour before stimulation.Primary control and depression model groups were perfused intragastrically with normal saline under similar conditions as the XYS groups.Three weeks later,all rats were sacrificed,and the expression levels of CNP and NPR-B in rectum tissues were analyzed by immunohistochemistry,real-time polymerase chain reaction,and Western blotting.RESULTS:CNP and NPR-B were both expressed in the rectum tissues of all rats.However,the expression levels of CNP and NPR-B at both gene and protein levels in the depression model group were significantly higher when compared to the primary control group(n = 9; P < 0.01).XYS intervention markedly inhibited the expression levels of CNP and NPR-B in depressed rats.The expression levels of CNP and NPR-B in the high dose XYS group did not significantly differ from the expression levels in the primary control group.Additionally,the high and middle dose XYS groups(but not the low dose group) significantly exhibited lower CNP and NPR-B expression levels in the rectum tissues of the respectively treated rats compared to the untreated depression model cohort(n = 9; P < 0.01).CONCLUSION:The CNP/NPR-B pathway is upregulated in the rectum of depressed rats and may be one mechanism for depression-associated digestive disorders.XYS antagonizes this pathway at least partially.

COPD患者治疗前后血清BNP.CNP和血浆Fbg·D-D水平检测的临床意义

目的:探讨了COPD患者治疗前后血清BNP·CNP和血浆Fbg·D-D水平的变化及临床意义。方法应用放射免疫分析法和生化法对36例COPD患者进行了治疗前后血清BNP·CNP和血浆Fbg·D-D水平检测,并与35名正常健康人作比较。结果 COPD患者在治疗前血清BNP·CNP和血浆Fbg·D-D水平均非常显著地高于正常人组(<0.01)经治疗2w后正常人比较仍有显著性差异(<0.05),血清BNP与CNP、Fbg·D-D水平呈正相关(r=0.5914,0.6018,0.4982<0.01)。结论检测COPD患者治疗前后血清BNP、CNP和血浆Fbg·D-D水平的变化对准确评估病情和预后具有重要的临床意义。

急性颅脑损伤早期血浆CNP和脑脊液乳酸测定的临床意义

目的 :探讨了血浆C -型利钠多肽 (CNP)和脑脊液乳酸水平在急性颅脑损伤早期的病理、生理作用。方法 :分别应用放射免疫分析血浆CNP水平和生化法测定脑脊液乳酸水平。结果 :在治疗前颅脑损伤患者血浆CNP水平非常显著地低于正常人组 (p <0 0 1 ) ,脑脊液乳酸水平明显高于正常人水平 (p <0 0 1 ) ,治疗 1 5天后与正常人比较无显著差异 (p >0 0 5 )。 结论 :观察血浆CNP和脑脊液乳酸水平的变化对研究急性颅脑损伤早期的病理生理变化。

Correlation of serum CNP and IGF-Ⅱ contents with brain injury and inflammatory response in patients with craniocerebral trauma

Objective:To study the correlation of serum C-type natriuretic peptide (CNP) and insulin-like growth factor-Ⅱ (IGF-Ⅱ) contents with brain injury and inflammatory response in patients with craniocerebral trauma.Methods: Patients with craniocerebral trauma who were treated in the First Affiliated Hospital of Xi'an Jiaotong University between March 2015 and July 2017 were included in the case group of the study, and the healthy volunteers who received physical examination during the same period were included in the control group. The contents of CNP, IGF-Ⅱ, nerve markers and pro-inflammatory cytokines in serum as well as the expression of inflammatory signaling molecules in peripheral blood were measured.Results: CNP and IGF-Ⅱ contents in serum of case group were significantly lower than those of control group whereas UCH-L1, GFAP, S100B, Tau, MIP-1α, IL-1β, IL-6, IL-8 and TNF-α contents in serum as well as JAK2, STAT3, MEK and ERK1/2 mRNA expression in peripheral blood were significantly higher than those of control group;CNP and IGF-Ⅱ contents in serum of case group were negatively correlated with UCH-L1, GFAP, S100B, Tau, MIP-1α, IL-1β, IL-6, IL-8 and TNF-α contents in serum as well as JAK2, STAT3, MEK and ERK1/2 mRNA expression in peripheral blood.Conclusion: The decrease of serum CNP and IGF-Ⅱ in patients with craniocerebral trauma is closely related to the aggravation of brain injury and the over-activation of inflammatory response.

高原低氧与C型利钠肽的关系探讨

目的 :探讨进驻高原不同海拔高度不同居住时间健康青年血浆C型利钠肽 (C -typenatriureticpeptideCNP)和心房利钠肽(AtrialnatriureticpeptideANP)的变化。方法 :对从平原进驻海拔 370 0m和 5 380m高原第 7d及 6个月的 40名边防某部官兵 ,应用放射免疫分析法检测其血浆CNP、ANP含量 ,并与 2 0名平原健康青年作对照。结果 :进驻海拔 370 0m和 5 380m的第 7天及 5 380m 6个月较平原CNP、ANP均增高显著 (P <0 .0 5或P <0 .0 1) ,370 0m居住 6个月时较平原ANP增高显著 (P <0 .0 1) ,CNP无统计学差异 (P >0 .0 5 )。且随海拔高度的升高而增高 (P <0 .0 5或P <0 .0 1) ,初入高原时明显高于在高原居住 6个月者 (P <0 .0 1)。结论 :CNP和ANP对急、慢性高原缺氧环境下的心血管系统功能调节具有重要意义。

三种钠尿肽抑制大鼠肺动脉平滑肌细胞增殖效应的比较

本文比较了心房钠尿肽 (ANP)、C 型钠尿肽 (CNP)、血管钠肽 (VNP)抑制肺动脉平滑肌细胞 (PASMCs)增殖的效应。用蛋白激酶C激动剂佛波酯 (PMA)刺激体外培养大鼠PASMCs的增殖 ,以总蛋白含量和MTT比色OD值为指标 ,观察三种钠尿肽对PMA刺激大鼠PASMCs增殖的影响。结果表明 ,PMA (10 -9～ 10 -7mol/L)显著升高(P <0 0 5 )PASMCs的总蛋白含量和MTTOD值 ,VNP (10 -8～ 10 -6mol/L)、ANP和CNP (10 -7～ 10 -6mol/L)均可显著降低 (P <0 0 5 )PMA (10 -8mol/L)刺激PASMCs的总蛋白含量和MTTOD值。结果提示 :PMA对大鼠肺动脉平滑肌细胞有促增殖作用 ,ANP、CNP、VNP均可抑制PMA刺激大鼠肺动脉平滑肌细胞的增殖 ,VNP的抑制效应最强。

C-型利钠肽调控鸡肌内脂肪细胞脂肪沉积的作用机制研究

本研究旨在探讨C型利钠肽(CNP)对鸡胸肌组织肌内前脂肪细胞的增殖、分化和分解的调控作用及机制,为进一步阐明肌内脂肪沉积的分子机制奠定基础。以黄羽肉鸡胸肌组织的肌内前脂肪细胞作为体外研究模型,利用10-7 mol/L CNP激素外源诱导前脂肪细胞,采用CCK8、Edu染色法观察肌内前脂肪细胞增殖的变化,油红O染色和异丙醇萃取法观察脂肪分化和沉积的变化;利用试剂盒检测细胞内cGMP及甘油浓度变化;实时荧光定量PCR检测利钠肽受体A、B、C(NPRA、NPRB和NPRC)的表达变化。结果显示,与对照组相比,CNP诱导前脂肪细胞1和3 d后,细胞的增殖能力显著升高(P<0.05);CNP诱导3和6 d后,诱导组脂肪细胞的分化和脂滴沉积能力极显著降低(P<0.01),释放到培养基的甘油浓度及细胞中的cGMP的浓度极显著升高(P<0.01)。NPRB和NPRC基因mRNA的表达极显著和显著高于对照组(P<0.01;P<0.05),NPRA基因mRNA的表达无显著变化(P>0.05)。本试验结果表明,CNP通过NPRB/NPRC-cGMP通路调控肉鸡肌内脂肪细胞的脂代谢过程。

正常妊娠、妊高征血浆C型利钠肽水平的变化

目的 :比较不孕龄妇女血浆C型利钠肽 (CNP)水平的变化 ,探讨CNP在妊高征发病机制中的可能作用。方法 :采用放射免疫分析法 ,对门诊及住院的 193例正常妊娠妇女、89例妊高征患者和 46例正常非孕妇女血浆中CNP浓度检测。结果 :早、中和晚期妊娠三组比较 ,血浆CNP差值有极显著性意义 (F =10 84,P <0 0 1) ,早孕组的血浆CNP值显著升高 ,中晚期无变化。不同程度妊高征组之间血浆CNP水平有极显著差别 (F =6 6 17,P <0 0 1)。与正常妊娠对照组相比 ,轻度妊高征者血浆CNP水平降低 ,中度者接近妊娠对照 ,重度者显著升高。正常妇女、正常妊娠、妊高征血浆CNP水平有显著性差别 (F =13 6 1,P <0 0 1) ,主要表现在妊高征血浆CNP值明显升高 ,正常妇女与正常妊娠组间无显著性差别。结论 :在生理状态下 ,CNP以自分泌和旁分泌的形式参与妊娠生理的调节 ;孕期血浆CNP变化特点 :早期升高 ,中晚期无变化。病理状态下 ,CNP作为循环激素 ,与妊高征病理生理变化密切相关。血浆CNP由低到高的变化反映妊高征的病程 ,可作为生化指标监测病情发展 ,指导妊高征的临床治疗及预后判断。

冠心病患者血清C型钠尿肽的变化及其临床意义

目的:探讨冠心病(CHD)患者血清C型钠尿肽(CNP)的变化及临床意义。方法:采用放射免疫分析测定了116例CHD患者和40例正常对照组血清CNP水平,进行对照统计分析。结果:CHD组血清CNP水平显著地高于正常对照组(t=6.368,P<0.01),Ⅰ、Ⅱ、Ⅲ及Ⅳ级心功能组血清CNP水平显著高于好转出院组(t=8.452,P<0.01)。结论:CHD组血清CNP显著升高,并随心功能分级递增,死亡者增加更明显。

EH患者血清利钠多肽及血栓素B_2水平的分析

目的:探讨原发性高血压(EH)患者血清ANP、BNP、CNP及TXB2水平的变化及其临床意义。方法:30例EH患者和正常人对照组分为2组;不同EH分期Ⅰ、Ⅱ、Ⅲ期分为3组,将测定结果进行统计分析。四项血清标志物均采用放射免疫分析。结果:EH患者组ANP、BNP、CNP及TXB2四项血清指标水平均较对照组升高非常显著(P均<0.01)。Ⅰ期组EH患者血清ANP、CNP及TXB2三项血清标志物与对照组比较差异均无显著性(P均>0.05),而血清BNP则显著高于对照组(P<0.05);Ⅱ期组患者ANP水平显著高于Ⅰ期组和对照组(P<0.05),而BNP、CNP和TXB2三项血清标志物则非常显著高于Ⅰ期组和对照组(P均<0.01)。Ⅲ期组结果显示,ANP、BNP、CNP和TXB2四项血清标志物均非常显著地高于Ⅱ期组、Ⅰ期组及对照组(P均<0.01),相关分析结果显示,患者ANP、BNP和CNP三项指标与自身的平均动脉压呈显著正相关(r=0.298,P<0.01;r=0.409,P<0.01;r=0.412,P<0.01)。结论:本文四项血清指标水平显著升高,测定数据的变化与EH的发病及病情进展有关。

血液透析中C型利钠肽、心脏扩张肽的连续动态观察

目的 通过对血液透析过程中CNP(c -typenatriureticpeptide)、CDN(cardiodilatin)的连续动态观测 ,对单次血液透析的影响作出评估。方法 选取 2 0 0 0年 6月～ 2 0 0 1年 8月在我中心长期维持性血液透析全部患者 18例 ,透析前血肌酐 >5 0 0 μmol/L ,其中男 14例 ,女 4例 ,年龄 2 9～ 74岁 (平均 4 8.4岁 ) ,血液透析时间 2个月～ 5 5个月 (平均 17个月 ) ,每次血液透析 5小时 ,每周 2～ 3次 ;血液透析器为德国BraunHE14 0 0 ,血液透析液为碳酸氢盐透析液。随机观测 30次血液透析过程 ,选取血液透析开始前、血液透析治疗第 1、2、3、4小时及血液透析结束时 (第 5小时 )共 6个时间 ,记录血压及采取血标本。用放免法检测CNP及CDN。数据按血液透析中标本采取时间分为 6个组进行自身组间对照 ,因素间行直线相关分析。结果 ①CNP正常参考值 14 .4 0～ 2 5 .5 2ng/L[(19.96± 5 .5 6 )ng/L]。血液透析前较血液透析中各组均低 ,有显著性差异 ;血液透析过程中及血液透析后各组间无显著性差异。各组均数均明显高于正常参考值。②CDN正常参考值 2 10～ 2 6 0ng/L。从均数上显示血液透析前、透析后较高 ,超出正常参考值上限 ,透析中降低 ,透析中各组均数均在正常参考值内 ,各组间两两比较无显著性差异。

血浆C型利钠肽在充血性心力衰竭中的临床价值

目的 探讨血浆C型利钠肽 (CNP)在充血性心力衰竭 (CHF)中的临床价值。方法 对120例CHF患者和38例健康者 ,用放射免疫法测定空腹血浆CNP含量。结果 CHF组血浆CNP含量明显高于正常对照组 (P<0.01),但不同心功能NYHA分级之间CNP含量无显著性差异(P>0.05)。结论 血浆CNP含量在CHF患者中明显升高 ,但与心衰程度无关 。