目的:建立甜瓜蒂 HPLC 数字化指纹图谱。方法:采用反相高效液相色谱法,使用 Century SIL C_(18)BDS 柱(200mm×4.6mm,5μm),以0.1%磷酸(含2mg·mL^(-1)庚烷磺酸钠)-乙腈(含0.1%磷酸)为流动相,梯度洗脱,流速1.0 mL·min^(-1)...目的:建立甜瓜蒂 HPLC 数字化指纹图谱。方法:采用反相高效液相色谱法,使用 Century SIL C_(18)BDS 柱(200mm×4.6mm,5μm),以0.1%磷酸(含2mg·mL^(-1)庚烷磺酸钠)-乙腈(含0.1%磷酸)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长228nm,柱温(30.0±0.15)℃,进样量5μL。以色谱指纹图谱指数(F)、色谱指纹图谱信息量指数(I)、投影含量相似度(C)和定量相似度(P)等数字化指标,定性、定量评价了不同产地甜瓜蒂药材质量。结果:以葫芦素 B 为参照物峰,通过测定10批药材指纹图谱确定20个共有峰,建立了甜瓜蒂 HPLC 数字化指纹图谱。结论:所建立的指纹图谱具有较好的精密度和重现性,可作为甜瓜蒂药材质量控制和真伪鉴别的依据。展开更多
Melon(C.melo L.)is an economically important vegetable crop cultivated worldwide.The melon collection in the U.S.National Plant Germplasm System(NPGS)is a valuable resource to conserve natural genetic diversity and pr...Melon(C.melo L.)is an economically important vegetable crop cultivated worldwide.The melon collection in the U.S.National Plant Germplasm System(NPGS)is a valuable resource to conserve natural genetic diversity and provide novel traits for melon breeding.Here we use the genotyping-by-sequencing(GBS)technology to characterize 2083 melon accessions in the NPGS collected from major melon production areas as well as regions where primitive melons exist.Population structure and genetic diversity analyses suggested that C.melo ssp.melo was firstly introduced from the centers of origin,Indian and Pakistan,to Central and West Asia,and then brought to Europe and Americas.C.melo ssp.melo from East Asia was likely derived from C.melo ssp.agrestis in India and Pakistan and displayed a distinct genetic background compared to the rest of ssp.melo accessions from other geographic regions.We developed a core collection of 383 accessions capturing more than 98%of genetic variation in the germplasm,providing a publicly accessible collection for future research and genomics-assisted breeding of melon.Thirty-five morphological characters investigated in the core collection indicated high variability of these characters across accessions in the collection.Genome-wide association studies using the core collection panel identified potentially associated genome regions related to fruit quality and other horticultural traits.This study provides insights into melon origin and domestication,and the constructed core collection and identified genome loci potentially associated with important traits provide valuable resources for future melon research and breeding.展开更多
文摘目的:建立甜瓜蒂 HPLC 数字化指纹图谱。方法:采用反相高效液相色谱法,使用 Century SIL C_(18)BDS 柱(200mm×4.6mm,5μm),以0.1%磷酸(含2mg·mL^(-1)庚烷磺酸钠)-乙腈(含0.1%磷酸)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长228nm,柱温(30.0±0.15)℃,进样量5μL。以色谱指纹图谱指数(F)、色谱指纹图谱信息量指数(I)、投影含量相似度(C)和定量相似度(P)等数字化指标,定性、定量评价了不同产地甜瓜蒂药材质量。结果:以葫芦素 B 为参照物峰,通过测定10批药材指纹图谱确定20个共有峰,建立了甜瓜蒂 HPLC 数字化指纹图谱。结论:所建立的指纹图谱具有较好的精密度和重现性,可作为甜瓜蒂药材质量控制和真伪鉴别的依据。
基金This research was supported by grants from USDA National Institute of Food and Agriculture Specialty Crop Research Initiative(2015-51181-24285 and 2020-51181-32139).
文摘Melon(C.melo L.)is an economically important vegetable crop cultivated worldwide.The melon collection in the U.S.National Plant Germplasm System(NPGS)is a valuable resource to conserve natural genetic diversity and provide novel traits for melon breeding.Here we use the genotyping-by-sequencing(GBS)technology to characterize 2083 melon accessions in the NPGS collected from major melon production areas as well as regions where primitive melons exist.Population structure and genetic diversity analyses suggested that C.melo ssp.melo was firstly introduced from the centers of origin,Indian and Pakistan,to Central and West Asia,and then brought to Europe and Americas.C.melo ssp.melo from East Asia was likely derived from C.melo ssp.agrestis in India and Pakistan and displayed a distinct genetic background compared to the rest of ssp.melo accessions from other geographic regions.We developed a core collection of 383 accessions capturing more than 98%of genetic variation in the germplasm,providing a publicly accessible collection for future research and genomics-assisted breeding of melon.Thirty-five morphological characters investigated in the core collection indicated high variability of these characters across accessions in the collection.Genome-wide association studies using the core collection panel identified potentially associated genome regions related to fruit quality and other horticultural traits.This study provides insights into melon origin and domestication,and the constructed core collection and identified genome loci potentially associated with important traits provide valuable resources for future melon research and breeding.
