Cytochrome c is a component of mitochondrial respiratory chain, located at the outer side of mitochondrial inner membrane. Its precursor, apocytochrome c, is encoded by a nuclear gene, synthesized on cytoplasmic ribos...Cytochrome c is a component of mitochondrial respiratory chain, located at the outer side of mitochondrial inner membrane. Its precursor, apocytochrome c, is encoded by a nuclear gene, synthesized on cytoplasmic ribosomes, and posttranslationally imported into mitochondria, but apocytochrome c is unique in the translocation compared with most mitochondrial proteins. It does not carry a cleavable amino terminal targeting sequence; no proteinous receptor on the mitochondrial outer membrane is identified for its import and its translocation does not compete with other preproteins for translocation machinery in the outer membrane. Besides, neither ATP nor membrane potential is required for its translocation across mitochonctria.展开更多
以羧甲基纤维素钠（CM C）为功能单体,K N O3为模板分子,18-冠醚-6（18-C-6）为致孔剂,去除模板离子（K（C））后制备了钾离子印迹膜层（K-IIM）.以戊二醛（G A）改性壳聚糖（CS）得到阴膜液,将阴膜液流延于上述K-IIM上形成了K（C）离...以羧甲基纤维素钠（CM C）为功能单体,K N O3为模板分子,18-冠醚-6（18-C-6）为致孔剂,去除模板离子（K（C））后制备了钾离子印迹膜层（K-IIM）.以戊二醛（G A）改性壳聚糖（CS）得到阴膜液,将阴膜液流延于上述K-IIM上形成了K（C）离子印迹双极膜（K-IIBPM）.采用扫描电镜（SEM）观察了K-IIBPM的形貌.K-IIM具有沟壑状的孔穴结构,孔穴半径约为0.130~0.170 nm,与K（C）的半径（0.137~0.164 nm）和形状相匹配,且具有规则性;经戊二醛改性后的阴膜层具有不规则的粗糙纹理.采用已循环使用十次的K-IIBPM富集海水中的钾,当海水中N a：K：Ca：M g的质量浓度（g L-1）比为10.70：0.41：0.42：1.32,压力差为0.03 M Pa,渗透时间为60 m in时,K（C）的透过率高达96%,其它被分离离子的截留率均在85%以上,从而实现富集海水中钾的目的.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 39730130).
文摘Cytochrome c is a component of mitochondrial respiratory chain, located at the outer side of mitochondrial inner membrane. Its precursor, apocytochrome c, is encoded by a nuclear gene, synthesized on cytoplasmic ribosomes, and posttranslationally imported into mitochondria, but apocytochrome c is unique in the translocation compared with most mitochondrial proteins. It does not carry a cleavable amino terminal targeting sequence; no proteinous receptor on the mitochondrial outer membrane is identified for its import and its translocation does not compete with other preproteins for translocation machinery in the outer membrane. Besides, neither ATP nor membrane potential is required for its translocation across mitochonctria.
文摘以羧甲基纤维素钠（CM C）为功能单体,K N O3为模板分子,18-冠醚-6（18-C-6）为致孔剂,去除模板离子（K（C））后制备了钾离子印迹膜层（K-IIM）.以戊二醛（G A）改性壳聚糖（CS）得到阴膜液,将阴膜液流延于上述K-IIM上形成了K（C）离子印迹双极膜（K-IIBPM）.采用扫描电镜（SEM）观察了K-IIBPM的形貌.K-IIM具有沟壑状的孔穴结构,孔穴半径约为0.130~0.170 nm,与K（C）的半径（0.137~0.164 nm）和形状相匹配,且具有规则性;经戊二醛改性后的阴膜层具有不规则的粗糙纹理.采用已循环使用十次的K-IIBPM富集海水中的钾,当海水中N a：K：Ca：M g的质量浓度（g L-1）比为10.70：0.41：0.42：1.32,压力差为0.03 M Pa,渗透时间为60 m in时,K（C）的透过率高达96%,其它被分离离子的截留率均在85%以上,从而实现富集海水中钾的目的.
