AIM: To investigate whether CD4(+)CD25(+) regulatory T (Treg) cells play a role in the development of anterior chamber-associated immune deviation (ACAID). METHODS: The dynamic changes in the frequency of CD4(+)D25(+)...AIM: To investigate whether CD4(+)CD25(+) regulatory T (Treg) cells play a role in the development of anterior chamber-associated immune deviation (ACAID). METHODS: The dynamic changes in the frequency of CD4(+)D25(+) T cells, CD4(+)D25(+) FoxP3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells from spleens of mice with ACAID were analyzed by flow cytometry. Foxp3 mRNA expression in purified CD4(+)CD25(+) T cells was analyzed using real-time PCR. The suppressive effect of purified CD4(+)CD25(+) T cells on the proliferation of CD4(+)CD25(-) T cells was evaluated by [H-3] thymidine incorporation. A blocking experiment was performed to further address the role of CD4(+)CD25(+) T cells in ACAID. The expression of IL-10 in purified CD4(+)CD25(+) T cells was evaluated by ELISA. RESULTS: Increased frequencies of CD4(+)CD25(+) T cells, CD4(+)CD25(+) Foxp3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells were observed in ACAID. The CD4(+)CD25(+) T cells from mice with ACAID showed enhanced suppressive effect on the proliferation of CD4(+)CD25(-) T cells. Treatment of BALB/c mice with anti-CD25 antibody after injection of OVA into the anterior chamber significantly inhibited the induction of ACAID. Furthermore, purified CD4(+)CD25(+) T cells from ACAID mice secreted IL-10. CONCLUSION: Our results demonstrate that Treg cells are induced in the mice undergoing ACAID. These Treg cells may play a role in the development of ACAID.展开更多
目的探讨参苓白术散对胃癌IV期患者外周血CD4+CD25+Tregs、Foxp3 m RNA及血清细胞因子IL-10、TGF-β1的影响。方法将40例胃癌IV期患者随机分为观察组、对照组各20例。对照组予对症支持治疗,观察组对症支持治疗同时予参苓白术散治疗,治疗...目的探讨参苓白术散对胃癌IV期患者外周血CD4+CD25+Tregs、Foxp3 m RNA及血清细胞因子IL-10、TGF-β1的影响。方法将40例胃癌IV期患者随机分为观察组、对照组各20例。对照组予对症支持治疗,观察组对症支持治疗同时予参苓白术散治疗,治疗4 w后观察两组患者外周血CD4+CD25+Tregs、单核细胞Foxp3 m RNA及血清IL-10、TGF-β1浓度的变化。结果治疗后观察组患者外周血CD4+CD25+Tregs、单核细胞Foxp3 m RNA及血清IL-10、TGF-β1浓度均显著降低(P<0.05),对照组治疗后各项指标无明显变化(P>0.05),两组治疗后比较差异有统计学意义(P<0.05)。结论参苓白术散能降低胃癌IV期患者外周血CD4+CD25+Tregs和单核细胞Foxp3 m RNA的表达,降低抑制性细胞因子IL-10、TGF-β1浓度,具有免疫调节作用。展开更多
基金National Natural Science Foundation of China(No. 30400487)International Cooperation Project of Guangdong Province, China (No. 2004B50301002) "1135" Talent Doctor Foundation of Daping Hospital,China (No. 2008-2010)
文摘AIM: To investigate whether CD4(+)CD25(+) regulatory T (Treg) cells play a role in the development of anterior chamber-associated immune deviation (ACAID). METHODS: The dynamic changes in the frequency of CD4(+)D25(+) T cells, CD4(+)D25(+) FoxP3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells from spleens of mice with ACAID were analyzed by flow cytometry. Foxp3 mRNA expression in purified CD4(+)CD25(+) T cells was analyzed using real-time PCR. The suppressive effect of purified CD4(+)CD25(+) T cells on the proliferation of CD4(+)CD25(-) T cells was evaluated by [H-3] thymidine incorporation. A blocking experiment was performed to further address the role of CD4(+)CD25(+) T cells in ACAID. The expression of IL-10 in purified CD4(+)CD25(+) T cells was evaluated by ELISA. RESULTS: Increased frequencies of CD4(+)CD25(+) T cells, CD4(+)CD25(+) Foxp3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells were observed in ACAID. The CD4(+)CD25(+) T cells from mice with ACAID showed enhanced suppressive effect on the proliferation of CD4(+)CD25(-) T cells. Treatment of BALB/c mice with anti-CD25 antibody after injection of OVA into the anterior chamber significantly inhibited the induction of ACAID. Furthermore, purified CD4(+)CD25(+) T cells from ACAID mice secreted IL-10. CONCLUSION: Our results demonstrate that Treg cells are induced in the mice undergoing ACAID. These Treg cells may play a role in the development of ACAID.
文摘目的探讨参苓白术散对胃癌IV期患者外周血CD4+CD25+Tregs、Foxp3 m RNA及血清细胞因子IL-10、TGF-β1的影响。方法将40例胃癌IV期患者随机分为观察组、对照组各20例。对照组予对症支持治疗,观察组对症支持治疗同时予参苓白术散治疗,治疗4 w后观察两组患者外周血CD4+CD25+Tregs、单核细胞Foxp3 m RNA及血清IL-10、TGF-β1浓度的变化。结果治疗后观察组患者外周血CD4+CD25+Tregs、单核细胞Foxp3 m RNA及血清IL-10、TGF-β1浓度均显著降低(P<0.05),对照组治疗后各项指标无明显变化(P>0.05),两组治疗后比较差异有统计学意义(P<0.05)。结论参苓白术散能降低胃癌IV期患者外周血CD4+CD25+Tregs和单核细胞Foxp3 m RNA的表达,降低抑制性细胞因子IL-10、TGF-β1浓度,具有免疫调节作用。
