Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel reproduction.Methods:Testicular tissue samples were obtained from 12 drom...Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel reproduction.Methods:Testicular tissue samples were obtained from 12 dromedary bull camels aged 7 to 8 at a local abattoir.The histological assessment involved tissue processing,hematoxylin and eosin(H&E)staining,and examination under a microscope.Stereological analysis,germ cell identification,and assessment of seminiferous tubules and maturation were conducted.Testosterone assay was performed by radioimmunoassay using blood samples collected at regular intervals.Results:The study revealed 12 phases of the dromedary camel's seminiferous epithelium cycle,highlighting distinct morphological characteristics and cellular processes.Acrosomal migration,maturation,cap formation,and the Golgi-mediated synthesis of proacrosomal vesicles were also explained in dimension,as were the steps of acrosome biogenesis.Spermatids and mature sperm cells were present when spermatogenesis phases were examined.An analysis of the dimensions of seminiferous tubules revealed specific measures for diameter,area,and epithelial height about luminal characteristics.Moreover,there were noticeable variations in the serum testosterone concentrations during the study period,indicating temporal dynamics.Conclusions:This study outlines the spermatogenesis process in dromedary camels across 12 stages,emphasizing cellular dynamics and acrosomal biogenesis.It also provides seminiferous tubule measurements and observes seasonal testosterone fluctuations,offering insights into reproductive regulation and potential strategies for camel breeding conservation.展开更多
Research shows that producing fermented camel milk is hard because of the milk’s inability to form a firm coagulum, attributed to low levels of κ-casein and ꞵ-lactoglobulin and the large casein micelle size, leading...Research shows that producing fermented camel milk is hard because of the milk’s inability to form a firm coagulum, attributed to low levels of κ-casein and ꞵ-lactoglobulin and the large casein micelle size, leading to a weak network of casein formation. In an effort to address this issue, researchers turned to corn starch as a thickening agent, discovering that a concentration of 2.0% effectively improved the viscosity and significantly reduced syneresis in stirred camel milk yoghurt and cultured camel milk. This study explores alternatives to corn starch, focusing on butternut squash seeds as a promising substitute due to their hydrocolloid composition. By incorporating butternut squash (Cucurbita moschata) seed powder (BSSP) as a thickening agent, this study aimed at enhancing the chemical and rheological properties of stirred camel milk yoghurt and cultured camel milk. Fermented camel milk was prepared using 4 litres of camel milk, 2% starter cultures (thermophilic culture for yoghurt and mesophilic aromatic culture for stirred cultured camel milk) and BSSP 0.0% (negative control), 0.4%, 0.8%, 1.2%, 1.6%, 2.0% mixed with 0.4% gelatin. 2.0% corn starch mixed with 0.4% gelatin was used as a standard for comparison. Results showed that increasing the BSSP level significantly (p < 0.05) decreased the moisture content while increasing the total solid content of stirred fermented camel milk products. There was an increase in ash content with an increase in BSSP levels. There was a significant (p < 0.05) reduction in the pH, with an increase in BSSP levels in stirred fermented camel milk samples. Increasing the concentration of BSSP from 0.4% to 2.0% resulted in a significant (p < 0.05) increase in viscosity and a reduction in syneresis of stirred camel milk yoghurt and stirred cultured camel milk samples. This study demonstrated that BSSP effectively enhances the viscosity, reduces syneresis and increases acidity in stirred fermented camel milk products during storage.展开更多
The study took a comprehensive approach to understanding mastitis in lactating camels, a disease that significantly impacts milk quantity and quality. This complex and multifactorial disease poses a significant challe...The study took a comprehensive approach to understanding mastitis in lactating camels, a disease that significantly impacts milk quantity and quality. This complex and multifactorial disease poses a significant challenge to dairy camel farming, particularly in Somalia. The studys objective was to determine the prevalence of mastitis and the risk factors associated with it among lactating camels on dairy farms in Benadir, Somalia. To achieve this, a cross-sectional study was conducted from May to September 2022 in the Benadir region of Somalia. A total of 96 lactating camels underwent examination using the California Mastitis Test (CMT) to identify clinical and subclinical mastitis cases. Additionally, a questionnaire survey was conducted among 20 farm employees/owners to gather information on hygiene practices, parity, lactation stage, tick infestation, and the presence of udder/teat lesions. Data regarding parity and lactation stage were obtained through owner interviews. The data collected on specifically designed forms were recorded and entered into a Microsoft Excel spreadsheet for analysis using SPSS version 20 statistical software, ensuring a comprehensive and reliable analysis of the data. The studys findings revealed a significant prevalence of camel mastitis, with 34.4% (33/96) of lactating camels affected and clinical and subclinical cases constituting 5.2% and 29.2%, respectively. Quarter-level prevalence was 46.3%, with clinical and subclinical mastitis at 4.7% and 41.6%, respectively. The study also identified significant associations (P < 0.05) among risk factors such as stage of lactation, parity, and age, indicating a higher susceptibility to mastitis in older camels during early lactation compared to those in late lactation and younger camels. These findings underscore the crucial role of inadequate hygienic conditions on camel farms and udder tick infestations in driving the elevated prevalence of mastitis, highlighting the need for improved management practices in dairy farming in Benadir, Somalia. Effective interventions, including improved management practices and extension services, are essential to reduce the burden of mastitis in dairy camels.展开更多
Camel plays a pivotal role in the subsistence pastoral economy of diverse ecozones extending from Gobi Desert and India in central Asia to Somalia and Ethiopia in the horn of Africa.Camel has special attributes includ...Camel plays a pivotal role in the subsistence pastoral economy of diverse ecozones extending from Gobi Desert and India in central Asia to Somalia and Ethiopia in the horn of Africa.Camel has special attributes including its appearance and ability to survive in hot,harsh and versatile arid environments.Camel has fascinated mankind as it can tolerate many stresses like heat;scarcity of water;water with high salinity and shortage of feed.Camel can digest dry matter and coarse crude fiber better than any other ruminants.Among domestic animals,the dromedary is most important animal being survive in hot,arid and semi-arid regions and has potential to produce higher quality foods(meat and milk)under extreme environments at lower costs.Camel can tolerate solar radiations,higher temperatures and water scarcity.Camel consume those feed materials which remain un-utilized by other domestic animals,thus thrive well on sandy deserts with poor vegetation.Adaptation of Camelids in Pakistan is very well to their native environment as they are performing and well sustaining a life in hostile deserts.The dromedaries provide milk and meat to the pastorals and herders in those areas where the survival of other livestock species is very tough.So,camels equilibrate the food security chain in the deep deserts and provide nourishment to its keepers;proving it to be a good candidate of food security and sovereignty in the desert ecosystem.展开更多
This study was conducted to investigate the effect of lactic acid bacteria (LAB) activated lactoperoxidase system (LPs) on keeping quality of raw camel milk at room temperature. Camel milk samples were collected from ...This study was conducted to investigate the effect of lactic acid bacteria (LAB) activated lactoperoxidase system (LPs) on keeping quality of raw camel milk at room temperature. Camel milk samples were collected from Errer valley, Babile district of eastern Ethiopia. The level of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) for activation of LPs was optimized using different levels of exogenous H<sub>2</sub>O<sub>2</sub>. Strains of LAB (<span style="white-space:nowrap;"><i></span>Lactococcus lactis 22333<span style="white-space:nowrap;"></i></span>, <span style="white-space:nowrap;"><i></span>Weissella confusa<span style="white-space:nowrap;"></i></span> 22308, <span style="white-space:nowrap;"><i></span>W. confusa<span style="white-space:nowrap;"></i></span> 22282, <span style="white-space:nowrap;"><i></span>W. confusa<span style="white-space:nowrap;"></i></span> 22296, <span style="white-space:nowrap;"><i></span>S. Infatarius<span style="white-space:nowrap;"></i></span> 22279 and <span style="white-space:nowrap;"><i></span>S. lutetiensis<span style="white-space:nowrap;"></i></span> 22319) with H<sub>2</sub>O<sub>2</sub> producing properties were evaluated, and <i>W. confusa</i> 22282 was selected as the best strain to produce H<sub>2</sub>O<sub>2</sub>. Storage stability of the milk samples was evaluated through the acidification curves, titratable acidity (TA), total bacterial count (TBC) and coliform counts (CC) at storage times of 0, 6, 12, 18, 24 and 48 hours. The LP activity and the inhibitory effect of activated LPs were evaluated by growing <span style="white-space:normal;"><i></span>E. coli<span style="white-space:normal;"></i></span> in pasteurized and boiled camel milk samples as contaminating agent. Results indicated that the <span style="white-space:normal;"><i></span>W. confusa<span style="white-space:normal;"></i></span> 22282 activated LPs generally showed significantly (P < 0.05) slower rates of acidification, lactic acid production and lower TBC and CC during the storage time compared to the non-activated sample. The H<sub>2</sub>O<sub>2</sub> producing LAB and exogenous H<sub>2</sub>O<sub>2</sub> activated LPs in pasteurized camel milk significantly reduced the growth of <span style="white-space:normal;"><i></span>E. coli<span style="white-space:normal;"></i></span> population compared to non-activated pasteurized milk. Overall, the result of acid production and microbial analysis indicated that the activation of LPs by H<sub>2</sub>O<sub>2</sub> producing LAB (i.e. <span style="white-space:normal;"> </span><span style="white-space:normal;"><i></span>W. confusa<span style="white-space:normal;"></i></span> 22282) maintained the storage stability of raw camel milk. Therefore, it can be concluded that the activation of LPs by biological method using H<sub>2</sub>O<sub>2</sub> producing LAB can substitute the chemical activation method of LPs in camel milk.展开更多
The camel economy is of considerable importance for arid countries</span><span style="font-family:"">. In the last decade, studies about camel immune system and immune responses have recorde...The camel economy is of considerable importance for arid countries</span><span style="font-family:"">. In the last decade, studies about camel immune system and immune responses have recorded increasing interest. However, drawing a comprehensive picture of the camel immune system remains far from reached. A major part of this review is to cover the studies of the primary and secondary immune organs and the markers of the camel immune cells and certain lymphoid tissues. At the same time, immune responses to different diseases and the nature of effective immunity were included, with an emphasis on the most important zoonotic diseases in camels such as MERS CoV;brucellosis. New findings on the diversity mechanisms of camel immunoglobulin genes were addressed. However, detail of the mechanism of MHC-restricted cellular immunity and the mechanism of B lymphocyte activation in camels await further attention. Interestingly, the gross and the histological structure of the lymphoid tissues of the camel’s thymus, tonsils, and p</span><span style="font-family:"">eyer’s </span><span style="font-family:"">p</span><span style="font-family:"">atches</span><span style="font-family:""> have indicated significant differences from other animals in terms of structure and function. The most peculiar CD expression, such as </span><span style="font-family:"">LPAM-I</span><span style="font-family:"">,</span><span style="font-family:""> MAdCAM-1<b> </b></span><span style="font-family:"">and CX3CR1, in certain camel cells and tissues refers to possible extraordinary mechanisms of immune hemostasis in camel </span><span style="font-family:"">in </span><span style="font-family:"">comparison to other ruminants. The widely applied immunodiagnostic techniques to control camel diseases and to assist in improving the camel resistance were considered. Extensive studies of the camel immune system were greatly hampered by lack of specific reagents to camel markers and low funds in the field of camel immunology.展开更多
文摘Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel reproduction.Methods:Testicular tissue samples were obtained from 12 dromedary bull camels aged 7 to 8 at a local abattoir.The histological assessment involved tissue processing,hematoxylin and eosin(H&E)staining,and examination under a microscope.Stereological analysis,germ cell identification,and assessment of seminiferous tubules and maturation were conducted.Testosterone assay was performed by radioimmunoassay using blood samples collected at regular intervals.Results:The study revealed 12 phases of the dromedary camel's seminiferous epithelium cycle,highlighting distinct morphological characteristics and cellular processes.Acrosomal migration,maturation,cap formation,and the Golgi-mediated synthesis of proacrosomal vesicles were also explained in dimension,as were the steps of acrosome biogenesis.Spermatids and mature sperm cells were present when spermatogenesis phases were examined.An analysis of the dimensions of seminiferous tubules revealed specific measures for diameter,area,and epithelial height about luminal characteristics.Moreover,there were noticeable variations in the serum testosterone concentrations during the study period,indicating temporal dynamics.Conclusions:This study outlines the spermatogenesis process in dromedary camels across 12 stages,emphasizing cellular dynamics and acrosomal biogenesis.It also provides seminiferous tubule measurements and observes seasonal testosterone fluctuations,offering insights into reproductive regulation and potential strategies for camel breeding conservation.
文摘Research shows that producing fermented camel milk is hard because of the milk’s inability to form a firm coagulum, attributed to low levels of κ-casein and ꞵ-lactoglobulin and the large casein micelle size, leading to a weak network of casein formation. In an effort to address this issue, researchers turned to corn starch as a thickening agent, discovering that a concentration of 2.0% effectively improved the viscosity and significantly reduced syneresis in stirred camel milk yoghurt and cultured camel milk. This study explores alternatives to corn starch, focusing on butternut squash seeds as a promising substitute due to their hydrocolloid composition. By incorporating butternut squash (Cucurbita moschata) seed powder (BSSP) as a thickening agent, this study aimed at enhancing the chemical and rheological properties of stirred camel milk yoghurt and cultured camel milk. Fermented camel milk was prepared using 4 litres of camel milk, 2% starter cultures (thermophilic culture for yoghurt and mesophilic aromatic culture for stirred cultured camel milk) and BSSP 0.0% (negative control), 0.4%, 0.8%, 1.2%, 1.6%, 2.0% mixed with 0.4% gelatin. 2.0% corn starch mixed with 0.4% gelatin was used as a standard for comparison. Results showed that increasing the BSSP level significantly (p < 0.05) decreased the moisture content while increasing the total solid content of stirred fermented camel milk products. There was an increase in ash content with an increase in BSSP levels. There was a significant (p < 0.05) reduction in the pH, with an increase in BSSP levels in stirred fermented camel milk samples. Increasing the concentration of BSSP from 0.4% to 2.0% resulted in a significant (p < 0.05) increase in viscosity and a reduction in syneresis of stirred camel milk yoghurt and stirred cultured camel milk samples. This study demonstrated that BSSP effectively enhances the viscosity, reduces syneresis and increases acidity in stirred fermented camel milk products during storage.
文摘The study took a comprehensive approach to understanding mastitis in lactating camels, a disease that significantly impacts milk quantity and quality. This complex and multifactorial disease poses a significant challenge to dairy camel farming, particularly in Somalia. The studys objective was to determine the prevalence of mastitis and the risk factors associated with it among lactating camels on dairy farms in Benadir, Somalia. To achieve this, a cross-sectional study was conducted from May to September 2022 in the Benadir region of Somalia. A total of 96 lactating camels underwent examination using the California Mastitis Test (CMT) to identify clinical and subclinical mastitis cases. Additionally, a questionnaire survey was conducted among 20 farm employees/owners to gather information on hygiene practices, parity, lactation stage, tick infestation, and the presence of udder/teat lesions. Data regarding parity and lactation stage were obtained through owner interviews. The data collected on specifically designed forms were recorded and entered into a Microsoft Excel spreadsheet for analysis using SPSS version 20 statistical software, ensuring a comprehensive and reliable analysis of the data. The studys findings revealed a significant prevalence of camel mastitis, with 34.4% (33/96) of lactating camels affected and clinical and subclinical cases constituting 5.2% and 29.2%, respectively. Quarter-level prevalence was 46.3%, with clinical and subclinical mastitis at 4.7% and 41.6%, respectively. The study also identified significant associations (P < 0.05) among risk factors such as stage of lactation, parity, and age, indicating a higher susceptibility to mastitis in older camels during early lactation compared to those in late lactation and younger camels. These findings underscore the crucial role of inadequate hygienic conditions on camel farms and udder tick infestations in driving the elevated prevalence of mastitis, highlighting the need for improved management practices in dairy farming in Benadir, Somalia. Effective interventions, including improved management practices and extension services, are essential to reduce the burden of mastitis in dairy camels.
文摘Camel plays a pivotal role in the subsistence pastoral economy of diverse ecozones extending from Gobi Desert and India in central Asia to Somalia and Ethiopia in the horn of Africa.Camel has special attributes including its appearance and ability to survive in hot,harsh and versatile arid environments.Camel has fascinated mankind as it can tolerate many stresses like heat;scarcity of water;water with high salinity and shortage of feed.Camel can digest dry matter and coarse crude fiber better than any other ruminants.Among domestic animals,the dromedary is most important animal being survive in hot,arid and semi-arid regions and has potential to produce higher quality foods(meat and milk)under extreme environments at lower costs.Camel can tolerate solar radiations,higher temperatures and water scarcity.Camel consume those feed materials which remain un-utilized by other domestic animals,thus thrive well on sandy deserts with poor vegetation.Adaptation of Camelids in Pakistan is very well to their native environment as they are performing and well sustaining a life in hostile deserts.The dromedaries provide milk and meat to the pastorals and herders in those areas where the survival of other livestock species is very tough.So,camels equilibrate the food security chain in the deep deserts and provide nourishment to its keepers;proving it to be a good candidate of food security and sovereignty in the desert ecosystem.
文摘This study was conducted to investigate the effect of lactic acid bacteria (LAB) activated lactoperoxidase system (LPs) on keeping quality of raw camel milk at room temperature. Camel milk samples were collected from Errer valley, Babile district of eastern Ethiopia. The level of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) for activation of LPs was optimized using different levels of exogenous H<sub>2</sub>O<sub>2</sub>. Strains of LAB (<span style="white-space:nowrap;"><i></span>Lactococcus lactis 22333<span style="white-space:nowrap;"></i></span>, <span style="white-space:nowrap;"><i></span>Weissella confusa<span style="white-space:nowrap;"></i></span> 22308, <span style="white-space:nowrap;"><i></span>W. confusa<span style="white-space:nowrap;"></i></span> 22282, <span style="white-space:nowrap;"><i></span>W. confusa<span style="white-space:nowrap;"></i></span> 22296, <span style="white-space:nowrap;"><i></span>S. Infatarius<span style="white-space:nowrap;"></i></span> 22279 and <span style="white-space:nowrap;"><i></span>S. lutetiensis<span style="white-space:nowrap;"></i></span> 22319) with H<sub>2</sub>O<sub>2</sub> producing properties were evaluated, and <i>W. confusa</i> 22282 was selected as the best strain to produce H<sub>2</sub>O<sub>2</sub>. Storage stability of the milk samples was evaluated through the acidification curves, titratable acidity (TA), total bacterial count (TBC) and coliform counts (CC) at storage times of 0, 6, 12, 18, 24 and 48 hours. The LP activity and the inhibitory effect of activated LPs were evaluated by growing <span style="white-space:normal;"><i></span>E. coli<span style="white-space:normal;"></i></span> in pasteurized and boiled camel milk samples as contaminating agent. Results indicated that the <span style="white-space:normal;"><i></span>W. confusa<span style="white-space:normal;"></i></span> 22282 activated LPs generally showed significantly (P < 0.05) slower rates of acidification, lactic acid production and lower TBC and CC during the storage time compared to the non-activated sample. The H<sub>2</sub>O<sub>2</sub> producing LAB and exogenous H<sub>2</sub>O<sub>2</sub> activated LPs in pasteurized camel milk significantly reduced the growth of <span style="white-space:normal;"><i></span>E. coli<span style="white-space:normal;"></i></span> population compared to non-activated pasteurized milk. Overall, the result of acid production and microbial analysis indicated that the activation of LPs by H<sub>2</sub>O<sub>2</sub> producing LAB (i.e. <span style="white-space:normal;"> </span><span style="white-space:normal;"><i></span>W. confusa<span style="white-space:normal;"></i></span> 22282) maintained the storage stability of raw camel milk. Therefore, it can be concluded that the activation of LPs by biological method using H<sub>2</sub>O<sub>2</sub> producing LAB can substitute the chemical activation method of LPs in camel milk.
文摘The camel economy is of considerable importance for arid countries</span><span style="font-family:"">. In the last decade, studies about camel immune system and immune responses have recorded increasing interest. However, drawing a comprehensive picture of the camel immune system remains far from reached. A major part of this review is to cover the studies of the primary and secondary immune organs and the markers of the camel immune cells and certain lymphoid tissues. At the same time, immune responses to different diseases and the nature of effective immunity were included, with an emphasis on the most important zoonotic diseases in camels such as MERS CoV;brucellosis. New findings on the diversity mechanisms of camel immunoglobulin genes were addressed. However, detail of the mechanism of MHC-restricted cellular immunity and the mechanism of B lymphocyte activation in camels await further attention. Interestingly, the gross and the histological structure of the lymphoid tissues of the camel’s thymus, tonsils, and p</span><span style="font-family:"">eyer’s </span><span style="font-family:"">p</span><span style="font-family:"">atches</span><span style="font-family:""> have indicated significant differences from other animals in terms of structure and function. The most peculiar CD expression, such as </span><span style="font-family:"">LPAM-I</span><span style="font-family:"">,</span><span style="font-family:""> MAdCAM-1<b> </b></span><span style="font-family:"">and CX3CR1, in certain camel cells and tissues refers to possible extraordinary mechanisms of immune hemostasis in camel </span><span style="font-family:"">in </span><span style="font-family:"">comparison to other ruminants. The widely applied immunodiagnostic techniques to control camel diseases and to assist in improving the camel resistance were considered. Extensive studies of the camel immune system were greatly hampered by lack of specific reagents to camel markers and low funds in the field of camel immunology.