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主动网络的应用及具体实现:Bowman和CANEs 被引量:2
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作者 刘雅琴 俞时权 《计算机工程》 CAS CSCD 北大核心 2002年第4期157-158,231,共3页
主动网络是可编程网络。主动网络中的网络结点具有计算能力,它能对流经本地的数据进行计算和修改。介绍了主动网络(activenetwork)的应用,实现方案并通过具体实例Bowman和CANEs来阐述。
关键词 主动网络 Bowman canes 执行环境 节点操作系统 数据流 计算机网络
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基于插件的Bowman & CANEs服务合成
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作者 吴世嘉 朱学永 《微型机与应用》 2010年第20期66-69,共4页
主动网络允许用户通过节点嵌入代码定制网络服务,从而实现网络的可编程性,使灵活加载新业务成为可能。介绍了主动网络的概念,分析了Bowman和CANEs主动网络的实现方案。该方案提供了一种与传统操作系统中的可装载模块类似的扩展功能,通... 主动网络允许用户通过节点嵌入代码定制网络服务,从而实现网络的可编程性,使灵活加载新业务成为可能。介绍了主动网络的概念,分析了Bowman和CANEs主动网络的实现方案。该方案提供了一种与传统操作系统中的可装载模块类似的扩展功能,通过发送一系列带有配置信息的信令文件,动态定制用户自己的协议和服务。 展开更多
关键词 主动网络 Bowman canes 服务合成
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主动网络的实现方案:Bowman+ CANEs
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作者 胡瑛 凌晓峰 沈金龙 《计算机工程与科学》 CSCD 2001年第4期25-28,共4页
主动网络 ;网络体系结构 ;节点操作系统主动网络是一种新型的网络体系结构 ,它允许用户或应用向报文内嵌入代码来定制网络服务 ,实现网络可编程 ,使灵活加载新业务成为可能。主动网络技术是当前的研究热点 ,相关的技术和解决方案不断提... 主动网络 ;网络体系结构 ;节点操作系统主动网络是一种新型的网络体系结构 ,它允许用户或应用向报文内嵌入代码来定制网络服务 ,实现网络可编程 ,使灵活加载新业务成为可能。主动网络技术是当前的研究热点 ,相关的技术和解决方案不断提出。本文首先介绍了主动网络的概念和意义 ,然后分析了一种新型的主动网络实现方案 :Bowman+CANEs。该方案实现了主动网络体系的关键技术 展开更多
关键词 主动网络 体系结构 节点操作系统 Bowman+CANE 计算机网络
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Composite Panels from the Combination of Rice Husk and Wood Chips with a Natural Resin Based on Tannins Reinforced with Sugar Cane Molasses Intended for Building Insulation: Physico-Mechanical and Thermal Properties
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作者 Paul Nestor Djomou Djonga Rosellyne Serewane Deramne +2 位作者 Gustave Assoualaye Ahmat Tom Tégawendé Justin Zaida 《Journal of Materials Science and Chemical Engineering》 2024年第2期19-30,共12页
The objective of this work is to develop new biosourced insulating composites from rice husks and wood chips that can be used in the building sector. It appears from the properties of the precursors that rice chips an... The objective of this work is to develop new biosourced insulating composites from rice husks and wood chips that can be used in the building sector. It appears from the properties of the precursors that rice chips and husks are materials which can have good thermal conductivity and therefore the combination of these precursors could make it possible to obtain panels with good insulating properties. With regard to environmental and climatic constraints, the composite panels formulated at various rates were tested and the physico-mechanical and thermal properties showed that it was essential to add a crosslinker in order to increase certain solicitation. an incorporation rate of 12% to 30% made it possible to obtain panels with low thermal conductivity, a low surface water absorption capacity and which gives the composite good thermal insulation and will find many applications in the construction and real estate sector. Finally, new solutions to improve the fire reaction of the insulation panels are tested which allows to identify suitable solutions for the developed composites. In view of the flame tests, the panels obtained are good and can effectively combat fire safety in public buildings. 展开更多
关键词 Composite Panels Tannins Reinforced Sugar Cane Molasses Building Insulation Mechanical and Thermal Properties
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Analysis of microRNA Expression Characteristics Related to Low Temperature Stress in Chewing Cane
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作者 Minghui CHEN Shiping CHENG +1 位作者 Jiansheng WANG Junqing WANG 《Asian Agricultural Research》 2023年第8期52-54,66,共4页
[Objectives]To explore microRNA expression characteristics related to low temperature stress in chewing cane.[Methods]The research on miRNA under abiotic stress of sugarcane at home and abroad mainly focused on the ty... [Objectives]To explore microRNA expression characteristics related to low temperature stress in chewing cane.[Methods]The research on miRNA under abiotic stress of sugarcane at home and abroad mainly focused on the types and regulation of miRNA under cold,heat,drought,high salt,and mechanical stress.However,there are few studies on miRNA under low temperature stress in chewing cane.The target genes of miR394 and miR825 in chewing cane were predicted and functionally analyzed by bioinformatics technology.[Results]The results showed that the target genes of miR394 and miR825 were mainly members of the WRKY transcription factor family,involved in plant growth,development and stress resistance.Real-time fluorescence quantitative PCR analyzed the expression characteristics of target miRNA in different tissues of chewing cane at different periods of low temperature stress.[Conclusions]The results showed that the expression of chewing cane miR394 and miR408 had temporal and spatial specificity and tissue specificity,both of which could respond to low temperature stress with significant differential expression. 展开更多
关键词 Chewing cane Low temperature stress MICRORNA Target gene
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Candy cane syndrome:A systematic review
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作者 Ricardo Rio-Tinto Jorge Canena Jacques Devière 《World Journal of Gastrointestinal Endoscopy》 2023年第7期510-517,共8页
BACKGROUND Candy cane syndrome(CCS)is a condition that occurs following gastrectomy or gastric bypass.CCS remains underrecognized,yet its prevalence is likely to rise due to the obesity epidemic and increased use of b... BACKGROUND Candy cane syndrome(CCS)is a condition that occurs following gastrectomy or gastric bypass.CCS remains underrecognized,yet its prevalence is likely to rise due to the obesity epidemic and increased use of bariatric surgery.No previous literature review on this subject has been published.AIM To collate the current knowledge on CCS.METHODS A literature search was conducted with PubMed and Google Scholar for studies from May 2007,until March 2023.The bibliographies of the retrieved articles were manually searched for additional relevant articles.RESULTS Twenty-one articles were identified(135 patients).Abdominal pain,nausea/vomiting,and reflux were the most reported symptoms.Upper gastrointestinal(GI)series and endoscopy were performed for diagnosis.Surgical resection of the blind limb was performed in 13 studies with resolution of symptoms in 73%-100%.In surgical series,9 complications were reported with no mortality.One study reported the surgical construction of a jejunal pouch with clinical success.Six studies described endoscopic approaches with 100%clinical success and no complications.In one case report,endoscopic dilation did not improve the patient’s symptoms.CONCLUSION CCS remains underrecognized due to lack of knowledge about this condition.The growth of the obesity epidemic worldwide and the increase in bariatric surgery are likely to increase its prevalence.CCS can be prevented if an elongated blind loop is avoided or if a jejunal pouch is constructed after total gastrectomy.Diagnosis should be based on symptoms,endoscopy,and upper GI series.Blind loop resection is curative but complex and associated with significant complications.Endoscopic management using different approaches to divert flow is effective and should be further explored. 展开更多
关键词 Candy cane syndrome Blind pouch syndrome Post-gastrectomy syndromes Side-to-side enteral anastomosis End-to-side enteral anastomosis
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不同施肥情况下甘蔗叶片的光谱特征分析及叶绿素含量检测(英文) 被引量:4
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作者 李修华 陈晓 +3 位作者 周永华 农梦玲 刘小阳 艾矫燕 《农业工程学报》 EI CAS CSCD 北大核心 2015年第S2期118-123,共6页
In order to investigate the visible-NIR spectral features of the leaves of a sugarcane variety(ROC 22),the spectral reflectance and the chlorophyll content were measured in the laboratory,and their correlations were a... In order to investigate the visible-NIR spectral features of the leaves of a sugarcane variety(ROC 22),the spectral reflectance and the chlorophyll content were measured in the laboratory,and their correlations were analyzed.Prediction models were built eventually.Results showed that the negative correlations with r greater than 0.8 was found in 527-578 nm and 701-731nm between spectral reflectance and chlorophyll content.And the red edge position(REP)was also found having high correlation with chlorophyll content,with the highest r of 0.8442.In order to explore the most sensitive bands for normalized difference vegetation index(NDVI),48471 NDVI values were computed with different wavebands for each sample and their correlations with chlorophyll were also analyzed.The distribution maps of NDVI and its correlations in a two-spectral-dimension space both indicated that the red bands had significant influence than the NIR bands.The suggested sensitive red range was 710-735 nm,especially720-725 nm;and the sensitive NIR range was from 780-850 nm,which had the higher robustness.The chlorophyll predication model with NDVI(725 and 840 nm)in tillering stage had determination coefficients of 0.7386,and was recommended for guiding the subsequent ridging fertilization. 展开更多
关键词 CHLOROPHYLL SUGAR CANE spectrum analysis NDVI sens
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一个简单海气耦合模式预报性能评估 被引量:7
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作者 岳彩军 陆维松 陶丽 《南京气象学院学报》 CSCD 北大核心 2005年第5期704-709,共6页
对改进初始强迫风场后的Zebiak-Cane海气耦合模式预报性能进行了全面评估.结果表明:1)耦合模式在20世纪90年代预报能力小于80年代;提前0~5个月的耦合模式预报能力小于同期持续预报能力,之后则相反;耦合模式对Nino3区指数预报能力最强.2... 对改进初始强迫风场后的Zebiak-Cane海气耦合模式预报性能进行了全面评估.结果表明:1)耦合模式在20世纪90年代预报能力小于80年代;提前0~5个月的耦合模式预报能力小于同期持续预报能力,之后则相反;耦合模式对Nino3区指数预报能力最强.2)在1997/1998年El Nino事件期间,耦合模式对东太平洋SSTA场预报能力大于其对中西太平洋SSTA场的预报能力,且提前0~2个月之后的耦合模式对东太平洋SSTA场预报能力远远大于持续预报. 展开更多
关键词 Zebiak—Cane海气耦合模式 NCEP/NCAR再分析风应力距平 预报性能
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PBMV IN TREATING MITRAL STENOSIS WITH LEFT ATRIAL THROMBUS
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作者 Yu Lingfan Hou Jingbo Su Guibin Jiao Jishan XuJiahua Wang Fengmei Guan Zhenzhong The second affiliated hospital of Harbin Medical University (150086) 《中国介入心脏病学杂志》 1998年第4期177-177,共1页
We have performed 348 canes of PBMV(pereutsneous Balloon Mitralvalvhloplssty)since April 1989,in which there are 4 Patients(13.8%)diagnosed as mitral stenosis with left atrial thrombus.To keepprothrombin time 1.5-2 ti... We have performed 348 canes of PBMV(pereutsneous Balloon Mitralvalvhloplssty)since April 1989,in which there are 4 Patients(13.8%)diagnosed as mitral stenosis with left atrial thrombus.To keepprothrombin time 1.5-2 times as normal,two kiods of thrombolytictherapy was qiven to these 48 patients,one was using 2 weeks ofurokinase(20 theusand units/d intravaneously)for early 18 cases,theother was using 4 weeks of warlarin(3-10mg/d.po)for other 30cases.PBMV was perlormed after thrombolytic therapy.In those 48cases,PBMV of 47 cases were successful,none of them had thecomplication,such as cerebral,limb and systemic emboesm.Though 1ease of PBMV was failed because balloon couldn’t be sent to mitralorifice owing to thrombus just adjacent to mitral orifice,which wasverified by intraesophagesl echocardiogram after operation,butseveral times of touching between balloon and thrombus hadn’tresulted in other organ’s embolism.Above data revealled that it’s safeto take PBMV after 4 weeks of thrombolytic therapy of warfarin inpatients of mitral stenosis with left atrial thrombus.The mechanismperhaps is,we think,that thrombolylic therapy can make freshthrombua dissolute and make old ones organize to prevent thrombusfrom dropping.So the patients of mitral stenosis with left atrialthrombus can take PBMV after strict thrombolytic therapy. 展开更多
关键词 PBMV THROMBUS MITRAL stenosis balloon orifice DROPPING STRICT canes think
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Imagery in Jean Toomer's Reapers
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作者 刘洋 《海外英语》 2014年第20期209-210,共2页
Jean Toomer was an important figure in African-American literature. He was an American poet,novelist and an important figure of the Harlem Renaissance. His most famous work,Cane,was published in 1923 and considered by... Jean Toomer was an important figure in African-American literature. He was an American poet,novelist and an important figure of the Harlem Renaissance. His most famous work,Cane,was published in 1923 and considered by many as a masterpiece of the Harlem Renaissance. Reapers is the first poem in Cane which is related to the reapers in southern plantation and rich in imagery. Toomer conveys profound theme by using various images in Reapers. 展开更多
关键词 Jean Toomer IMAGERY Reapers CANE
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Correlation and Path Coefficient Analyses in Sugarcane Genotypes of Ethiopia 被引量:6
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作者 Esayas Tena Firew Mekbib Amsalu Ayana 《American Journal of Plant Sciences》 2016年第10期1490-1497,共8页
To study the relations of certain morphological and sugar quality characters with sugarcane yield, an experiment was conducted at Wonji and Metehara Sugar Estates, Ethiopia between March 2012 and October 2013. The exp... To study the relations of certain morphological and sugar quality characters with sugarcane yield, an experiment was conducted at Wonji and Metehara Sugar Estates, Ethiopia between March 2012 and October 2013. The experiment, comprising of 400 sugarcane genotypes of which 174 were local that were collected from different regional states of Ethiopia and 226 introduced, was laid out in partial balanced lattice design with two replications. Data was collected on cane yield and its components, sugar yield and sugar quality traits. ANOVA, correlation and path coefficient analysis were done. Analysis of variance revealed significant differences (P < 0.01) for all the characters studied. Cane yield showed strong positive and highly significant (P < 0.01) correlation with millable cane number (r = 0.832), single cane weight (r = 0.528), stalk height (r = 0.517) and sugar yield (r = 0.987). There was also positive significant (P = 0.05) correlation of tiller count and cane diameter with cane yield. Path analysis revealed the highest positive direct effect of millable cane number (0.812) on cane yield followed by single cane weight (0.682) and pol percent (0.550). However, stalk diameter and brix percent had considerable negative direct effects and indirect positive effects through single cane weight on cane yield. Therefore, in view of their significant positive association with cane yield, indirect effects of stalk diameter and brix percent via single cane weight should be considered during selection. Genotypes should be selected on the basis of millable cane number, single cane weight and pol percent for getting higher cane and sugar yield. 展开更多
关键词 Cane Yield Morphological Characters Sugar Quality Sugar Yield
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XPS and FTIR studies of fungus-stained Daemonorops margaritae 被引量:1
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作者 Wenhua Lyu Yuan Shi +1 位作者 Yaxian Zheng Xing'e Liu 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第2期739-743,共5页
We explored the discoloration of rattan cane using X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FTIR). XPS analysis showed that after the cane was stained by Lasiodiplodia theobrom... We explored the discoloration of rattan cane using X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FTIR). XPS analysis showed that after the cane was stained by Lasiodiplodia theobromae, carbon and oxygen elements and the ratio of oxygen to carbon decreased. Considering atomic binding,C_1 and C_4 contents increased, while C_2 and C_3 contents decreased, and the ratio of O_2 to O_1 decreased sharply. The relative contents of lignin, cellulose and polysaccharides increased and new substances with low O_2/O_1 ratio occurred. FTIR analysis showed that the absorption peaks of O–H at 3346 cm^(-1), aliphatic C–H at 2921, 2853 and1464 cm^(-1), and C=O at 1723 cm^(-1), were characteristic peaks of fungal melanin intensified, indicating that cane discoloration was primarily caused by fungal melanin. The absorption peaks characterizing cellulose and lignin like polysaccharides at 800 cm^(-1), C–H at 1374 cm^(-1), C–O at1058 and 1038 cm^(-1), phenolic hydroxyl at 1245 cm^(-1),aromatic ether bonds at 1270 cm^(-1), carbon skeleton at1608 cm^(-1) and benzene ring at 1500 cm^(-1) were enhanced since the fungus mainly consumed the extractives in cane cell lumens and the main composition content increased relatively. Regardless of the discoloration caused by natural fungi or inoculated fungi, the discoloring feature and composition changes were identical except that the fungusinoculated cane had more melanin. 展开更多
关键词 RATTAN CANE FUNGAL stain FUNGAL MELANIN FTIR XPS
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宽带网络—有线电视HFC网 被引量:3
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作者 徐宗保 《科技资讯》 2005年第24期144-145,共2页
本文对有线电视HFC网络的现状和技术特征描述之后,着重介绍了在HFC网络上通过CableModem开展宽带业务的情况,并且谈到如何改造HFC网络这一话题。
关键词 有线电视 HFC网 宽带业务 CANE Modem 有线电视HFC网 宽带网络 Cable HFC网络 特征描述
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Residue of Organphosphorus Pesticides in Chewing Cane Juices Detected by QuEChERS and GC-NPD Method 被引量:1
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作者 Fan Yegeng Liao Jie +5 位作者 Liang Jun Mo Leixing Wang Tianshun Ya Yu Liang Qiang Li Song 《Plant Diseases and Pests》 CAS 2013年第4期8-11,38,共5页
[ Objective] The paper was to establish a quick detection method for residue of organphosphoras pesticides in chewing cane juices and provide guarantee for edible safety of chewing juice. [ Method] By using QuEChERS a... [ Objective] The paper was to establish a quick detection method for residue of organphosphoras pesticides in chewing cane juices and provide guarantee for edible safety of chewing juice. [ Method] By using QuEChERS and GC-NPD method, a rapid analysis method for residue of six kinds of organphosphorus pesti- cides (dichlorves, chlorpyrifos-methyl, malathion, fenthion, chlorpyrifos and triazophes) in sugarcane juices was established. [ Resdt] QuEChERS method was significantly superior to traditional extraction method and could extract six kinds of organphesphorus pesticides from chewing cane juices effectively, which gready saved organic reagent and time. Good linearity was found in different standard curves; when the concentration of pesticides was from 0. 020 toO. 100 rag/L, the re- covery rate was from 68.58% to 92.85%, and relative standard deviation (RSD) was ranged from 2.16% to 9.89%. [ Conclusion] The sensitivity, accuracy and precision of QuEChERS method were consistent with the technical requirements of pesticide residue determination. It not only could shorten sample processing cycle effectively, but also ensure that no impurity was in the sample peak and the recovery rate was also consistent with the requirements of residual analysis. 展开更多
关键词 Chewing cane Organphesphoras pesticides GC-NPD QUECHERS RESIDUE
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Climate Superiority of Sugar Cane Planting in Longzhou County of Guangxi 被引量:1
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作者 Aizhen CHENG Xingji ZENG Zhongxiong HUANG 《Asian Agricultural Research》 2013年第11期42-45,共4页
According to meteorological conditions for sugar cane growth and development,surface meteorological observation data of Longzhou County in 1981-2010 were taken as basis,to analyze influence of local temperature,precip... According to meteorological conditions for sugar cane growth and development,surface meteorological observation data of Longzhou County in 1981-2010 were taken as basis,to analyze influence of local temperature,precipitation and sunshine conditions on sugar cane planting.Results show that unique climate of Longzhou County is extremely suitable for sugar cane planting and growth. 展开更多
关键词 SUGAR CANE PLANTING CLIMATE Analysis Longzhou Coun
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Isolation of lactic acid bacteria from sugar cane juice and production of lactic acid from selected improved strains 被引量:1
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作者 Yoganand Sobrun Archana Bhaw-Luximon +1 位作者 Dhanjay Jhurry Daneshwar Puchooa 《Advances in Bioscience and Biotechnology》 2012年第4期398-407,共10页
Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocreso... Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocresol purple and sucrose. However, only 17 isolates which formed large yellow areas were selected for further investigations. These isolates were further identified according to their morphological and biochemical characteristics. It was found that 10 of these isolates were homofermenters. One of these 10 isolates was selected for mutagenesis using chemical (Ethidium bromide) and physical (UV-B) mutagens followed by biochemical characterisation. A total of 112 mutants were isolated and 9 homofermentative isolates were further investigated for their ability to produce lactic acid. 1H-NMR spectroscopy confirmed that all mutant isolates produced lactic acid as the sole fermentation product. 展开更多
关键词 LACTIC Acid BACTERIA SUGAR CANE JUICE MUTAGENESIS
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Influence of Substrate Feeding and Process Parameters on Production of Coenzyme Q<sub>10</sub>Using <i>Paracoccus denitrificans</i>ATCC 19367 Mutant Strain P-87 被引量:1
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作者 Pradipta Tokdar Prafull Ranadive +2 位作者 Rajendra Kshirsagar Samanta Shekhar Khora Sunil Kumar Deshmukh 《Advances in Bioscience and Biotechnology》 2014年第12期966-977,共12页
Coenzyme Q10 (CoQ10), an important antioxidant molecule playing a major role in electron transport chain, has been commercially produced by fermentation process for the use in oral nutraceutical formulations. Construc... Coenzyme Q10 (CoQ10), an important antioxidant molecule playing a major role in electron transport chain, has been commercially produced by fermentation process for the use in oral nutraceutical formulations. Constructing the high-yielding CoQ10 producing strains is a pre-requisite for cost-effective production. A superior mutant strain P-87 generated from Paracoccus denitrificans ATCC 19367, which showed 1.25-fold improvement in specific CoQ10 content higher than the wild type strain at shake flask level, was selected to carry out the studies on CoQ10 yield improvement through fermenter process optimization. In the course of study, initially the cane-molasses-based medium and fed-batch fermentation strategy using pHBA in combination with sucrose were standardized in shake flask using wild type strain. This strategy was subsequently translated at 2 L laboratory fermenter while optimizing the fermentation process parameters using improved mutant strain P-87. Under optimized fermentation condition, mutant strain P-87 produced 49.85 mg/L of CoQ10 having specific content of 1.63 mg/g of DCW, which was 1.36 folds higher than the specific CoQ10 content of wild-type strain under similar optimized condition. The temperature and DO were found to be critical parameters for CoQ10 production by mutant strain P-87. The optimum temperature was found to be 32°C and the optimum DO concentration to be maintained throughout the fermentation cycle was found to be 30% of air saturation. Overall, a new cost-effective process has been established for the production of CoQ10 using the cheaper substrate “cane molasses” and higher CoQ10 producing mutant strain P-87. 展开更多
关键词 CoQ10 PARACOCCUS DENITRIFICANS Cane MOLASSES pHBA Sucrose DO Process Optimization
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Properties of Rattan Cane Reinforced Concrete Facade Subjected to Axial Loading 被引量:1
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《Journal of Civil Engineering and Architecture》 2011年第11期1048-1052,共5页
The use of steel reinforced concrete facade is gradually becoming unpopular because of the damage caused to the elements as a result of corrosion of the steel reinforcement. It is now possible to build lightweight and... The use of steel reinforced concrete facade is gradually becoming unpopular because of the damage caused to the elements as a result of corrosion of the steel reinforcement. It is now possible to build lightweight and slender concrete structures which are invulnerable to corrosion with the use of fiber reinforcements, which are gradually replacing the corrosion prone steel. This paper describes the construction of faqade panels made of rattan cane reinforcements and steel reinforcements, ten number model sized elements of area 0.15 m2 and a depth of 40 mm were considered, with five panels for each reinforcement type. The elements were subjected to incremental load of 1 kN until failure occurred. Deflections were measured for each element, and crack width was measured at failure load. The results for both types of reinforcements when compared showed the rattan cane reinforced panels failing earlier than the steel reinforced panels. However a lower defection and crack width was recorded in the rattan cane reinforced panel. This paper concludes that the lower crack width formed after failure in the rattan cane reinforced panel gave it advantage over the steel reinforced panel, since it has lower space for the ingress of water which is the main agents of corrosion. 展开更多
关键词 Rattan cane steel reinforcement CORROSION faqade crack width.
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Optimized Biosurfactant Production by <i>Pseudomonas aeruginosa</i>Strain CGA1 Using Agro-Industrial Waste as Sole Carbon Source 被引量:1
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作者 Chikodili G. Anaukwu Chioma M. Ogbukagu Ikechukwu A. Ekwealor 《Advances in Microbiology》 2020年第10期543-562,共20页
Biosurfactants are biomolecules produced by microorganisms, which possess several advantages over their chemical counterparts. Production can be cost-effective if renewable wastes are utilized as substrates. In this s... Biosurfactants are biomolecules produced by microorganisms, which possess several advantages over their chemical counterparts. Production can be cost-effective if renewable wastes are utilized as substrates. In this study, optimization of biosurfactant production by <i>Pseudomonas aeruginosa</i> strain CGA1 was carried out using response surface methodology. The conventional “One factor at a time” method of optimization was initially adopted to ascertain the impact of different renewable wastes on biosurfactant production. Four independent variables were tested: carbon and nitrogen concentration, medium volume, and inoculum size. Biosurfactant production was based on the emulsification index measurement. Results indicated that the preferred carbon source by the isolate was sugar cane molasses. A 2.31-fold increase in biosurfactant yield and emulsification index of 96.3% ± 0.75% under optimized cultural conditions of 20 g/L of molasses, 5 g/L of sodium nitrate, 1.93 ml inoculum size and 60 ml medium volume in 250 ml conical flask were obtained. The regression coefficient (R<sup>2</sup>) value of 84.15% implied adequate fitness of the model. The surface tension of distilled water was reduced from 72.1 mN/m to 35.0 ± 0.0 mN/m, and critical micelle concentration was attained at 60 mg·L<sup>-1</sup>. FTIR and GC-MS analysis indicated that the biosurfactant was a lipopeptide having characteristic lipid and peptide peak values. This study proves that the sole use of agro-industrial wastes for the production of biosurfactant is very efficient, and ensures the economic feasibility of biosurfactant production. 展开更多
关键词 Pseudomonas aeruginosa Optimization Response Surface Methodology BIOSURFACTANT Sugar Cane Molasses LIPOPEPTIDE
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Yields of Polyhydroxyalkanoates (PHAs) during Batch Fermentation of Sugar Cane Juice by Alcaligenes latus and Alcaligenes eutrophus 被引量:1
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作者 Waranya Suwannasing Samart Moonamart Pakawadee Kaewkannetra 《Journal of Life Sciences》 2011年第11期960-966,共7页
In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates (PHAs) by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·... In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates (PHAs) by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·L^-1 (sucrose 36.6g·L^-1 , fructose 26.0g·L^-1 , glucose 21.8g·L^-1 and other sugars 21.1g·L^-1 ). Each inoculums ( 10%, v/v) was separately cultivated in the medium containing 20g·L^-1 total sugars under condition (30℃, 200 rpm, pH 6.5-7). It was found that the A. eutrophus can be grown better than the A. latus. Only the A. eutrophus was further cultured under different total sugar concentrations (20, 30, 40 and 50g·L^-1 ). The optimal contents of total sugar, dry cell mass (DCM) and maximum PHAs were obtained at 50g·L^-1 , 6.013g·L^-1 and 1.84g·L^-1 , respectively after 60 h fermentation which were converted to biomass yield (Yx/s), product yield (Yp/5), specific product yield (Yp/x) and productivity of 0.163, 0.05, 0.306 and 0.031 g.Llhl. Large scale of PHAs production was conducted in 5 L fermentor using the optimal condition obtained under 30% dissolved oxygen. The DCM and the maximum PHAs were 5.881g·L^-1 and 1.281g·L^-1 which were calculated to values of Yx/s, Yp/s, Yp/x and productivity at 0.19, 0.04, 0.218 and 0.021g·L^-1 , respectively. 展开更多
关键词 A lcaligenes latus A lcaligenes eutrophus FERMENTATION PHAS sugar cane juice
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