The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants,and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyon...The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants,and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyond genome editing.Here,we review the most recent applications of the CRISPR/Cas toolkit in plants and also discuss key factors for improving CRISPR/Cas performance and strategies for reducing the off-target effects.Novel technical breakthroughs in mammalian research regarding the CRISPR/Cas toolkit will also be incorporated into this review in hope to stimulate prospective users from the plant research community to fully explore the potential of these technologies.展开更多
Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9(CRISPR/Cas9)system has recently become one popular technology due to its efficiency,precision,and simplicity compared with other genome edi...Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9(CRISPR/Cas9)system has recently become one popular technology due to its efficiency,precision,and simplicity compared with other genome editing tools such as Zinc Finger Nucleases(ZFNs)and Transcription Activator Like Effector Nucleases(TALENs).Horticultural crops provide energy and health-keeping nutrients to humankind.Genome-editing technology has become widely adopted in horticultural breeding with the increasing demand for high yield and better-quality horticultural crops.Here,we describe the CRISPR/Cas9 system construction,its optimization,including sgRNA promoter,sgRNA design,Cas9 protein promoter,SpCas9 variants and orthologs,and vector delivery methods.We also summarized the application of this technology in horticultural plants for stress responses enhancement,fruit quality improvement,and cultivation traits modification.This detailed review was compiled to help establish comprehensive understanding of the CRISPR/Cas9 systems and provide a reference for further developing this technology to manipulate horticultural plant traits effectively.展开更多
Ascientific research center on nanometer technology was set up on October 30, 2000 at the Chinese Academy of Sciences (CAS). The aim of the center, called CAS Center for Nanometer Science and Technology, will strive t...Ascientific research center on nanometer technology was set up on October 30, 2000 at the Chinese Academy of Sciences (CAS). The aim of the center, called CAS Center for Nanometer Science and Technology, will strive to enhance China’s research strength, realize its commercialization and increase its competitiveness in the field, says Prof. Bai Chunli, CAS Vice President.展开更多
Ⅰ. TWO YEARS’ WORK IN RETROSPECT 1. The 1955 By-election of CAS Membership At the annual session of the standing committee of our Division held in July 1995, some problems concerning the by-election were discussed. ...Ⅰ. TWO YEARS’ WORK IN RETROSPECT 1. The 1955 By-election of CAS Membership At the annual session of the standing committee of our Division held in July 1995, some problems concerning the by-election were discussed. At the meeting, a resolution on the "Methods for appraisal and election of candidates of CAS membership for technological sciences" was examined and adopted,in addition to a plenary decision on some other matters. At another plenary session of the展开更多
STATE ENGINEERING RESEARCH CENTER FOR ENGINEERING PLASTICS Affiliation: CAS Institute of Chemistry Working priorities: Oriented by market trends, the Center aims at probing into the development and commercialization o...STATE ENGINEERING RESEARCH CENTER FOR ENGINEERING PLASTICS Affiliation: CAS Institute of Chemistry Working priorities: Oriented by market trends, the Center aims at probing into the development and commercialization of engineering plastics,such as the development of competitive new materials,new technologies, new products and scale-up manufacturing expertise. Through technical transfers and knowledge dissem-展开更多
The recently developed RNA-guided clustered regularly interspaced short palindromic repeat （CRISPR）/CRISPR-associated 9 （Cas9） nuclease system has progressed to be an invaluable technology for genome manipulation ...The recently developed RNA-guided clustered regularly interspaced short palindromic repeat （CRISPR）/CRISPR-associated 9 （Cas9） nuclease system has progressed to be an invaluable technology for genome manipulation in somatic cell types and germline model organisms. While the unprecedented advance in human embryo gene editing research has great potential in next-generation therapeutics, it raises various ethical concerns that need to be addressed before being translated for clinical use. Here, we discuss the current and potential applications of CRISPR/Cas9 technology and its limitations in clinical applications, as well as ethical and legal considerations in the treatment, disease prevention or disability in somatic cells or human embryo via gene editing.展开更多
Dear Editor,Despite tremendous effort devoted to the development of antiretroviral therapies to combat HIV over the past decades, AIDS remains one of the most important global infectious diseases. According to UNAIDS ...Dear Editor,Despite tremendous effort devoted to the development of antiretroviral therapies to combat HIV over the past decades, AIDS remains one of the most important global infectious diseases. According to UNAIDS report on the global AIDS epidemic in 2016, the estimated number of people living with HIV rose from 7.5 million in 2010 to 36.7 million in 2015. Furthermore, drug-resistance HIV strains have recently been reported （Wensing et al., 2017）. Therefore, it is important to develop new therapies to eliminate HIV in the patients. Immortalized cell lines representing the major targets of HIV in human are important for HIV research and therapeutic development.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos. 31522006 and 31570276)funds from Sun Yatsen University and from China's Thousand Young Talents Program to J.-F.Li
文摘The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants,and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyond genome editing.Here,we review the most recent applications of the CRISPR/Cas toolkit in plants and also discuss key factors for improving CRISPR/Cas performance and strategies for reducing the off-target effects.Novel technical breakthroughs in mammalian research regarding the CRISPR/Cas toolkit will also be incorporated into this review in hope to stimulate prospective users from the plant research community to fully explore the potential of these technologies.
基金supported by grants from the National Key R&D Program of China (Grant No. 2018YFD1000100)National Natural Science Foundation of China (Grant No. 31972378)Agricultural Variety Improvement Project of Shandong Province (Grant No. 2019LZGC007)
文摘Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9(CRISPR/Cas9)system has recently become one popular technology due to its efficiency,precision,and simplicity compared with other genome editing tools such as Zinc Finger Nucleases(ZFNs)and Transcription Activator Like Effector Nucleases(TALENs).Horticultural crops provide energy and health-keeping nutrients to humankind.Genome-editing technology has become widely adopted in horticultural breeding with the increasing demand for high yield and better-quality horticultural crops.Here,we describe the CRISPR/Cas9 system construction,its optimization,including sgRNA promoter,sgRNA design,Cas9 protein promoter,SpCas9 variants and orthologs,and vector delivery methods.We also summarized the application of this technology in horticultural plants for stress responses enhancement,fruit quality improvement,and cultivation traits modification.This detailed review was compiled to help establish comprehensive understanding of the CRISPR/Cas9 systems and provide a reference for further developing this technology to manipulate horticultural plant traits effectively.
文摘Ascientific research center on nanometer technology was set up on October 30, 2000 at the Chinese Academy of Sciences (CAS). The aim of the center, called CAS Center for Nanometer Science and Technology, will strive to enhance China’s research strength, realize its commercialization and increase its competitiveness in the field, says Prof. Bai Chunli, CAS Vice President.
文摘Ⅰ. TWO YEARS’ WORK IN RETROSPECT 1. The 1955 By-election of CAS Membership At the annual session of the standing committee of our Division held in July 1995, some problems concerning the by-election were discussed. At the meeting, a resolution on the "Methods for appraisal and election of candidates of CAS membership for technological sciences" was examined and adopted,in addition to a plenary decision on some other matters. At another plenary session of the
文摘STATE ENGINEERING RESEARCH CENTER FOR ENGINEERING PLASTICS Affiliation: CAS Institute of Chemistry Working priorities: Oriented by market trends, the Center aims at probing into the development and commercialization of engineering plastics,such as the development of competitive new materials,new technologies, new products and scale-up manufacturing expertise. Through technical transfers and knowledge dissem-
文摘The recently developed RNA-guided clustered regularly interspaced short palindromic repeat （CRISPR）/CRISPR-associated 9 （Cas9） nuclease system has progressed to be an invaluable technology for genome manipulation in somatic cell types and germline model organisms. While the unprecedented advance in human embryo gene editing research has great potential in next-generation therapeutics, it raises various ethical concerns that need to be addressed before being translated for clinical use. Here, we discuss the current and potential applications of CRISPR/Cas9 technology and its limitations in clinical applications, as well as ethical and legal considerations in the treatment, disease prevention or disability in somatic cells or human embryo via gene editing.
文摘Dear Editor,Despite tremendous effort devoted to the development of antiretroviral therapies to combat HIV over the past decades, AIDS remains one of the most important global infectious diseases. According to UNAIDS report on the global AIDS epidemic in 2016, the estimated number of people living with HIV rose from 7.5 million in 2010 to 36.7 million in 2015. Furthermore, drug-resistance HIV strains have recently been reported （Wensing et al., 2017）. Therefore, it is important to develop new therapies to eliminate HIV in the patients. Immortalized cell lines representing the major targets of HIV in human are important for HIV research and therapeutic development.
