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Human bone marrow stromal cells in cooperation with exogenous cytokines support in vitro expansion of cord blood CD34^+ cells
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《中国输血杂志》 CAS CSCD 2001年第S1期411-,共1页
关键词 bone Human bone marrow stromal cells in cooperation with exogenous cytokines support in vitro expansion of cord blood cd34 cells cd
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Expression of Caspase-3 in Cord Blood CD34^+ Cells during Culture in vitro
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作者 马艳萍 邹萍 +1 位作者 肖娟 黄士昂 《The Chinese-German Journal of Clinical Oncology》 CAS 2003年第3期166-168,192,共4页
Objective: To investigate the expression and significance of caspase-3 protein in CD34^+ cells from cord blood (CB) during culture in vitro with different growth factors. Methods: RT-PCR, Western blot and flow cytomet... Objective: To investigate the expression and significance of caspase-3 protein in CD34^+ cells from cord blood (CB) during culture in vitro with different growth factors. Methods: RT-PCR, Western blot and flow cytometry techniques were used to detect the expression of caspase-3 in CD34^+ CB cells during culture in vitro. Results: Caspase-3 mRNA was constitutively expressed at a low level in freshly isolated CD34^+ cells. The expression of caspase-3 mRNA and protein was upregulated when these cellswere first expanded in suspension culture with growth factors for 3 days. However, only the 32 kDa inactive caspase-3 proenzyme was detected in the freshly isolated CD34^+ cells as well as during the first 3 days expansion with cytokines. With longer culture time in vitro, especially in the presence of the combination of IL-3, IL-6 and GM-CSF, caspase-3 was activated and a cleavage product of 20 kDa became detectable.Conclusion: Caspase-3 is involved in apoptosis of primitive CB CD34^+ cells during expansion in vitro. 展开更多
关键词 CASPASE-3 ^cd34^+ cells cord blood APOPTOSIS
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Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury 被引量:3
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作者 Ming-san Miao Lin Guo +1 位作者 Rui-qi Li Xiao Ma 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第2期278-284,共7页
Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the rec... Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the recombinant human granulocyte colony stimulating factor-mobilized bone marrow stem cell transplantation on cerebral ischemia/reperfusion injury. Rat ischemia models were administered 0.3, 0.15 and 0.075 g/kg Radix Ilicis Pubescentis total flavonoids from 3 days before modeling to 2 days after injury. Results showed that Radix Ilicis Pubescentis total flavonoids could reduce pathological injury in rats with cerebral ischemia/reperfusion injury. The number of Nissl bodies increased, Bax protein expression decreased, Bcl-2 protein expression increased and the number of CD34-positive cells increased. Therefore, Radix Ilicis Pubescentis total flavonoids can improve the bone marrow stem cell mobilization effect, enhance the anti-apoptotic ability of nerve cells, and have a neuroprotective effect on cerebral ischemia/reperfusion injury in rats. 展开更多
关键词 nerve regeneration Radix Ilicis Pubescentis total flavonoids bone marrow stem cells MOBILIZATION cerebral ischemia/reperfusion Nissl bodies Bax Bcl-2 cd34 neurons neural regeneration
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Occurrence of MYD88L265P and CD79B mutations in diffuse large b cell lymphoma with bone marrow infiltration:A case report
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作者 Wen-Ye Huang Zhi-Yun Weng 《World Journal of Clinical Cases》 SCIE 2022年第22期7994-8002,共9页
BACKGROUNDOver the past 20 years,we have gained a deep understanding of the biologicalheterogeneity of diffuse large B cell lymphoma(DLBCL)and have developed arange of new treatment programs based on the characteristi... BACKGROUNDOver the past 20 years,we have gained a deep understanding of the biologicalheterogeneity of diffuse large B cell lymphoma(DLBCL)and have developed arange of new treatment programs based on the characteristics of the disease,bringing us to the era of immune-chemotherapy.However,the effectiveness andmolecular mechanisms of targeted-immunotherapy remain unclear in DLBCL.Targeted-immunotherapy may be beneficial for specific subgroups of patients,thus requiring biomarker assessment.CASE SUMMARYHere,we report a case of MCD subtype DLBCL with MYD88L265P and CD79Bmutations,considered in the initial stage as lymphoplasmic lymphoma(LPL)orWaldenstrom macroglobulinemia(WM).Flow cytometry supported this view;however,the immunohistochemical results of the lymph nodes overturned theabove diagnosis,and the patient was eventually diagnosed with MCD subtypeDLBCL.The presence of a monoclonal IgM component in the serum and infiltrationof small lymphocytes with a phenotype compatible with WM into the bonemarrow led us to propose a hypothesis that the case we report may have transformedfrom LPL/WM.CONCLUSIONThis highlights the possible transformation from WM to DLBCL,CD79B mutationmay be a potential biomarker for predicting this conversion. 展开更多
关键词 bone marrow infiltration Case report cd79B Diffuse large B cell lymphoma Ibrutinib MYD88L265P
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Overexpression of vascular endothelial growth factor enhances the neuroprotective effects of bone marrow mesenchymal stem cell transplantation in ischemic stroke 被引量:5
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作者 Cui Liu Zhi-Xiang Yang +6 位作者 Si-Qi Zhou Ding Ding Yu-Ting Hu Hong-Ning Yang Dong Han Shu-Qun Hu Xue-Mei Zong 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第6期1286-1292,共7页
Although bone marrow mesenchymal stem cells(BMSCs)might have therapeutic potency in ischemic stroke,the benefits are limited.The current study investigated the effects of BMSCs engineered to overexpress vascular endot... Although bone marrow mesenchymal stem cells(BMSCs)might have therapeutic potency in ischemic stroke,the benefits are limited.The current study investigated the effects of BMSCs engineered to overexpress vascular endothelial growth factor(VEGF)on behavioral defects in a rat model of transient cerebral ischemia,which was induced by middle cerebral artery occlusion.VEGF-BMSCs or control grafts were injected into the left striatum of the infarcted hemisphere 24 hours after stroke.We found that compared with the stroke-only group and the vehicle-and BMSCs-control groups,the VEGF-BMSCs treated animals displayed the largest benefits,as evidenced by attenuated behavioral defects and smaller infarct volume 7 days after stroke.Additionally,VEGF-BMSCs greatly inhibited destruction of the blood-brain barrier,increased the regeneration of blood vessels in the region of ischemic penumbra,and reducedneuronal degeneration surrounding the infarct core.Further mechanistic studies showed that among all transplant groups,VEGF-BMSCs transplantation induced the highest level of brain-derived neurotrophic factor.These results suggest that BMSCs transplantation with vascular endothelial growth factor has the potential to treat ischemic stroke with better results than are currently available. 展开更多
关键词 bone marrow mesenchymal stem cell brain-derived neurotrophic factor cd31 microtubule associated protein 2 middle cerebral artery occlusion stroke transplantation vascular endothelial growth factor
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High percentage of bone marrow CD8^(+)tissue-resident-like memory T cells predicts inferior survival in patients with acute myeloid leukemia
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作者 Letong Cai Wenpu Lai +15 位作者 Danlin Yao Yinfeng Gu Chaofeng Liang Lian Liu Jing Lai Zhi Yu Xianfeng Zha Xibao Yu Xiuli Wu Shaohua Chen Oscar Junhong Luo Yangqiu Li Chunyan Wang Pengfei Qin Xin Huang Ling Xu 《Blood Science》 2024年第3期23-32,共10页
Tissue-resident memory T(TRM)cells infiltrating solid tumors could influence tumor progression and the response to immune therapies.However,the proportion and prognostic value of TRM cells in the bone marrow(BM)of pat... Tissue-resident memory T(TRM)cells infiltrating solid tumors could influence tumor progression and the response to immune therapies.However,the proportion and prognostic value of TRM cells in the bone marrow(BM)of patients with acute myeloid leukemia(AML)are unclear.In this study,we used flow cytometry to assay the phenotype of 49 BM samples from patients newly diagnosed with AML(ND-AML).We found that the BM CD8^(+)effector memory(TEM)cells highly expressed CD69(CD8^(+)TRM-like T cells),and their percentage was significantly increased in patients with ND-AML compared with that in healthy individuals(HI).The high percentage of CD8^(+)TRM-like subset was associated with poor overall survival in our ND-AML cohort.The Kaplan–Meier Plotter database verified a significantly reduced survival rate among patients with high expression of CD8^(+)TRM-like T cell characteristic genes(CD8A,CD69,and TOX),especially the M4 and M5 subtypes.Phenotypic analysis revealed that the BM CD8^(+)TRM-like subpopulation exhibited exhausted T cell characteristics,but its high expression of CD27 and CD28 and low expression of CD57 suggested its high proliferative potential.The single-cell proteogenomic dataset confirmed the existence of TRM-like CD8^(+)T cells in the BM of patients with AML and verified the high expression of immune checkpoints and costimulatory molecules.In conclusion,we found that the accumulation of BM CD8^(+)TRM-like cells could be an immune-related survival prediction marker for patients with AML. 展开更多
关键词 Acute myeloid leukemia bone marrow cd69 Tissue-resident-like memory T cells
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Relationship between bone marrow-derived CD_(34)^+ cells expressing interleukin-5 receptor messenger RNA and asthmatic airway inflammation 被引量:5
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作者 毛辉 王曾礼 +2 位作者 李富宇 刘春涛 雷松 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第1期24-29,共6页
BACKGROUND: Asthma is clinically related with the degree of eosinophilic inflammation. How asthmatic airway inflammation is affected is still poorly understood. So the effects of bone marrow-derived hematopoietic cell... BACKGROUND: Asthma is clinically related with the degree of eosinophilic inflammation. How asthmatic airway inflammation is affected is still poorly understood. So the effects of bone marrow-derived hematopoietic cells expressing CD(34) (CD(34)(+)) and interleukin-5 (IL-5) receptor messenger RNA (IL-5R mRNA+) on asthmatic airway inflammation were investigated. METHODS: Balb/c mice were sensitized and challenged by ovalbumin (OVA) to establish an asthmatic model while control mice were sensitized and exposed to sterile saline. The mice were killed at different time points after being challenged by OVA and sterile saline. Then, bronchoalveolar lavage fluid (BALF), peripheral blood (PB) and bone marrow (BM) were prepared. Eosinophils in PB (PBEOS) and BALF (BALFEOS), nuclear cells in BALF, PB and BM were counted. By flow cytometry, the percentage of CD(34)(+) cells to nucleated cells in PB, BM and the relative number of CD(34)(+) cells in PB (PBCD(34)(+)) and BM (BMCD(34)(+)) were calculated. Immunocytochemistry and in situ hybridization were used to investigate the hematopoietic cells with co-localized expression of CD(34) and IL-5R mRNA in BM (BMCD34+IL-5R mRNA+). The percentage of BMCD34+IL-5R mRNA+ to BMCD(34)(+) was calculated. RESULTS: Twelve hours after challenge by OVA, BALFEOS and PBEOS in the experimental group were significantly higher than those in the control group (P 展开更多
关键词 Animals Antigens cd34 Asthma bone marrow cells Bronchoalveolar Lavage Fluid Inflammation Male MICE Mice Inbred BALB C RNA Messenger Receptors Interleukin Research Support Non-U.S. Gov't
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Effect of Ligustrazine on CD_(34) Antigen Expression of Bone MarrowCells in Immune-Induced Aplastic Anemia Mice
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作者 舒砚君 孙汉英 +3 位作者 董凌莉 徐慧珍 路武 刘文励 《Chinese Journal of Integrative Medicine》 SCIE CAS 1998年第2期148-148,共1页
Objective:To explore the effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-induced aplastic anemia(AA)mice.Methods:The model of immune aplastic anemia mice was in duced by means of 6.0... Objective:To explore the effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-induced aplastic anemia(AA)mice.Methods:The model of immune aplastic anemia mice was in duced by means of 6.0GY60γ-rayirr adi at ion and lym phocyte infusion through tail vein.Immuneinduce dAA micewere divided into 3 groups:thenormal group,the AA control group and the ligustrazine group.Miceof the ligustrazine group were fed by 4 mg of ligustrazine in jecti on twicea day bygastro gavage.On the 10 thday,CD34 an tigen expression intensity of bone marrow cell membrane was measured by flowcy tometer an alysis system.Results:CD34 antigen expressionin tensity of ligustrazine group was 77.6±6.5,with no statistic difference from that in normal group(80.0±2.6),while that of the control group was muchhigher(68.6±4.5,P<0.05).Conclusion:Ligustrazine could promote proliferation of stem and progenitor cell of AA mice through in fluencing on bone marrowmicro-environment so as to increase the CD34antigen expression of bone marrow cells. 展开更多
关键词 Effect of Ligustrazine on cd cells in Immune-Induced Aplastic Anemia Mice Antigen Expression of bone marrow
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Effect of Homoharringtonine on Bone Morrow CD34^+CD7^+ Cells in Chronic Granulocytic Leukemia
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作者 李玉峰 邓之奎 +1 位作者 宣衡报 陈宝安 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2007年第2期141-145,共5页
Objective: To explore the effect of homoharringtonine (HHT) on bone morrow CD34^+CD7^+ cells in chronic granulocytic leukemia (CGL). Methods: The changes of bone morrow CD34^+CD7^+ cells were observed after ... Objective: To explore the effect of homoharringtonine (HHT) on bone morrow CD34^+CD7^+ cells in chronic granulocytic leukemia (CGL). Methods: The changes of bone morrow CD34^+CD7^+ cells were observed after the treatment of HHT in 23 cases with CGL. The proliferation and apoptosis of CD34^+CD7^+ cells treated with HHT in vitro were studied. Results: The proportion of CD34^+CD7^+ cells in CGL (0.145±0.021) was higher than that of normal control (0.052±0.013). The proportion of CD34^+CD7^+ cells in patients who got cytogenetic responses to HHT (0.072±0.020) decreased remarkably, but not in those patients who did not got cytogenetic responses to HHT, (0.137±0.023). the proliferation of CD34^+ cells was inhibited and the proportion of CD34^+CD7^+ cells decreased after cultured with HHT (0.134 in 24 h, 0.126 in 48 h and 0.102 in 72). The apoptosis rate of CD34^+CD7^+ cells was higher than that in CD34^+CDT cells (35.39%±4.39% versus 24.57%±4.01%, P〈0.05) 72 h after culture with HHT. Conclusion: The proportion of CD34^+CD7^+ cells in CGL was higher than that of normal control and HHT may inhibit the proliferation and induce apoptosis of bone marrow CD34^+CD7^+ cells. 展开更多
关键词 HOMOHARRINGTONINE Chronic granulocytic leukemia ^cd34^+cd7^+ cells
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Tumor-Infiltrating Lymphocytes in Primary Tumor and Bone Marrow in Patients with Gastric Cancer and Overweight
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作者 Larissa Nikitichna Bubnovskaya Viktor Michailovich Mikhailenko +1 位作者 Sergey Pavlovich Merentsev Dmitriy Sergeevich Osinsky 《Journal of Pharmacy and Pharmacology》 2019年第12期611-620,共10页
Immune infiltration in human tumors is a key prognostic factor.In this aspect it has to be noted that the publications concerning the tumor-infiltrating lymphocytes(TILs)under overweight and obesity are limited,especi... Immune infiltration in human tumors is a key prognostic factor.In this aspect it has to be noted that the publications concerning the tumor-infiltrating lymphocytes(TILs)under overweight and obesity are limited,especially in the clinical setting in particular concerning gastric cancer.This study has shown that density of TILs consistently associated with body mass index(BMI)and density of cancer-associated adipocytes(CAA)in tumors,decreased significantly in patients with BMI>30 having high density of CAA.Hypoxia in tumor does not affect this process.Patients with BMI>30 having high density of CAA with slight infiltrating of TILs in tumors demonstrated better overall survival than the same patients with BMI<25 whereas the same patients with BMI<25 but with low density of CAA have shown much longer overall survival.Presence of TILs in BM was not affected by both the high density of CAA and level of hypoxia in primary tumor;moreover it has not been associated with BMI as well as patients survival time.Understanding the metabolic changes that occur in obese individuals may help to pave the way for more effective treatments for patients with gastric cancer having overweight. 展开更多
关键词 ^cd8^+ ^cd45RO^+-T LYMPHOCYTES CAA bone marrow HYPOXIA BMI
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PERIPHERAL BLOOD CD34^+ CELL MOBILIZATION IN 42 PATIENTS WITH SEVERE AUTOIMMUNE DISEASE
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作者 Wei Zhang Dao-bin Zhou +8 位作者 Yan Zhao Jun-ling Zhuang Xiao-mei Leng Shu-jie Wang Li Jiao Fu-lin Tang Jie-ping Zhang Xuan Wang Ti Shen 《Chinese Medical Sciences Journal》 CAS CSCD 2007年第2期108-112,共5页
Objective To evaluate the feasibility and safety of peripheral CD34+ cell mobilization in patients with severe autoimmune disease. Methods Forty-two patients underwent a total of 46 mobilizations by the regimen of cyc... Objective To evaluate the feasibility and safety of peripheral CD34+ cell mobilization in patients with severe autoimmune disease. Methods Forty-two patients underwent a total of 46 mobilizations by the regimen of cyclophosphamide 2-3 g/m2 +recombinant human granulocyte colony stimulating factor (rhG-CSF) 5 μg·kg-1·d-1. The positive selection of CD34+ cell was performed through the CliniMACS. Results In 8.1±2.3 days after administration of cyclophosphamide, the peripheral white blood cell and mononuclear cell (MNC) decreased to the lowest level. In 3.7±1.6 days after injection of rhG-CSF, the peripheral absolute MNC and CD34+ cell counts were 0.95×109/L and 0.035×109/L, respectively. After 2.4±0.6 times of leukapheresis, there gained 4.46×108/kg of MNC and 5.26×106/kg of CD34+, respectively. After mobilization, the underlying diseases were ameliorated more or less. In systemic lupus erythematosus (SLE) patients, SLE Disease Activity Index (SLEDAI) decreased from a median of 17 to 3 (P<0.01). In rheumatic arthritis patients, an American College of Rheumatology criteria for 20%(ACR20) response was achieved in all five patients. Totally, 17.4% of patients whose absolute neutrophil count <0.5×109/L suffered infection, and 31.0% of patients had bone pain after the injection of rhG-CSF. Two patients suffered severe complications, one with acute renal failure and recovered by hemodialysis, the other died of thrombotic thrombocytopenic purpura. Failed mobilization occurred in three patients. Conclusions Sufficient CD34+ cells can be mobilized by low dose of cyclophosphamide and rhG-CSF. CD34+ cell mobilization for treatment of severe autoimmune disease not only is appropriate in both effectiveness and safety but ameliorates disease also. 展开更多
关键词 autoimmune disease ^cd34^+ cell mobilization
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G-CSF对健康人骨髓和外周血CD_(34)^+细胞和T细胞亚群影响的动态观察 被引量:3
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作者 王存邦 欧英贤 +4 位作者 白海 潘耀柱 欧建锋 慕海霞 王晓静 《西北国防医学杂志》 CAS 2003年第6期410-411,共2页
目的 :探讨G -CSF对健康人骨髓及外周血CD3 4 + 细胞和T细胞亚群的影响。方法 :对 12例健康人 ,以G -CSF15 0 μg/ 12h ,皮下注射 ,连用 6d。应用流式细胞仪测定CD3 4 + 和T细胞亚群。结果 :应用G -CSF4 8h后 ,骨髓CD3 4 + 细胞开始明... 目的 :探讨G -CSF对健康人骨髓及外周血CD3 4 + 细胞和T细胞亚群的影响。方法 :对 12例健康人 ,以G -CSF15 0 μg/ 12h ,皮下注射 ,连用 6d。应用流式细胞仪测定CD3 4 + 和T细胞亚群。结果 :应用G -CSF4 8h后 ,骨髓CD3 4 + 细胞开始明显增加 ,72h后外周血CD3 4 + 细胞开始明显增加 ,96h后两者达峰值 ,均较应用前差异显著 (P <0 .0 1)。T淋巴细胞亚群变化不明显 (P>0 .0 5 )。结论 :健康人应用G -CSF后骨髓及外周血CD3 4 + 细胞逐渐增加并于 96h后达到高峰 ,T细胞亚群则无明显变化。 展开更多
关键词 重组人粒细胞集落刺激因子 骨髓 外周血 ^cd34^+细胞 T淋巴细胞亚群
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应用单克隆抗体-免疫磁珠分离系统分离纯化再生障碍性贫血骨髓CD_(34)^+细胞
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作者 李公宝 孙绪江 +3 位作者 许福亮 纪恩美 马传香 王树庆 《潍坊医学院学报》 2003年第1期10-11,共2页
目的 分离、纯化再生障碍性贫血 (简称AA)患者骨髓CD3 4+ 细胞 ,探讨其发病机制。方法 对4 2例AA患者 ,以常规方法进行骨髓液采集和有核细胞分离 ,用单克隆抗体 免疫磁珠分离系统 (MACS)分离、纯化骨髓CD3 4+ 细胞 ,用碱性磷酸酶 ... 目的 分离、纯化再生障碍性贫血 (简称AA)患者骨髓CD3 4+ 细胞 ,探讨其发病机制。方法 对4 2例AA患者 ,以常规方法进行骨髓液采集和有核细胞分离 ,用单克隆抗体 免疫磁珠分离系统 (MACS)分离、纯化骨髓CD3 4+ 细胞 ,用碱性磷酸酶 抗碱性磷酸酶 (APAAP)法作CD3 4+ 细胞的纯度检测。结果 骨髓CD3 4+ 细胞纯度达 95 %~ 99% ,AA患者骨髓CD3 4+ 细胞多呈单个、散在分布 ,着色较淡 ;而对照组骨髓CD3 4+ 细胞多呈均匀或丛簇状分布 ,着色较深。结论 应用MACS分离AA骨髓CD3 4+ 细胞 ,特异性强、纯度高 ,是研究AA骨髓CD3 4+ 细胞功能、形态的最直接、客观。 展开更多
关键词 单克隆抗体—免疫磁珠分离系统 贫血 再生障碍性 骨髓 ^cd34^+ 细胞
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CD271 as a marker to identify mesenchymal stem cells from diverse sources before culture 被引量:4
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作者 María álvarez-Viejo Yolanda Menéndez-Menéndez Jesús Otero-Hernández 《World Journal of Stem Cells》 SCIE CAS 2015年第2期470-476,共7页
Mesenchymal stem cells, due to their characteristics are ideal candidates for cellular therapy. Currently, in culture these cells are defined by their adherence to plastic, specific surface antigen expression and mult... Mesenchymal stem cells, due to their characteristics are ideal candidates for cellular therapy. Currently, in culture these cells are defined by their adherence to plastic, specific surface antigen expression and multipotent differentiation potential. However, the in vivo identification of mesenchymal stem cells, before culture, is not so well established. Pre-culture identification markers would ensure higher purity than that obtained with selection based on adherence to plastic. Up until now, CD271 has been described as the most specific marker for the characterization andpurification of human bone marrow mesenchymal stem cells. This marker has been shown to be specifically expressed by these cells. Thus, CD271 has been proposed as a versatile marker to selectively isolated and expand multipotent mesenchymal stem cells with both immunosuppressive and lymphohematopoietic engraftment-promoting properties. This review focuses on this marker, specifically on identification of mesenchymal stem cells from different tissues. Literature revision suggests that CD271 should not be defined as a universal marker to identify mesenchymal stem cells before culture from different sources. In the case of bone marrow or adipose tissue, CD271 could be considered a quite suitable marker; however this marker seems to be inadequate for the isolation of mesenchymal stem cells from other tissues such as umbilical cord blood or wharton's jelly among others. 展开更多
关键词 MESENCHYMAL stem cells cd271 Lowaffinitynerve growth factor receptor P75 bone marrow
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原发性骨髓纤维化患者CD 34^+细胞水平变化及临床意义
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作者 王瑞娟 《中国继续医学教育》 2015年第26期24-25,共2页
目的探讨原发性骨髓纤维化患者外周血中CD 34+细胞水平变化及临床意义。方法选取我科收治的15例原发性骨髓纤维化患者作为观察组,15例健康志愿者作为对照组,运用免疫组织化学染色法检测两组人群外周血中CD 34+细胞百分比及单核细胞计数... 目的探讨原发性骨髓纤维化患者外周血中CD 34+细胞水平变化及临床意义。方法选取我科收治的15例原发性骨髓纤维化患者作为观察组,15例健康志愿者作为对照组,运用免疫组织化学染色法检测两组人群外周血中CD 34+细胞百分比及单核细胞计数,比较两组数据差异。结果原发性骨髓纤维化患者外周血中CD 34+细胞计数较对照组高,P<0.05,差异具有统计学意义。结论原发性骨髓纤维化患者外周血中CD 34+细胞数量较健康人高,可作为骨髓纤维化疾病的鉴别、诊断和病情预测标准,指导临床治疗方案的制定。 展开更多
关键词 骨髓纤维化 原发性 cd34+细胞 临床意义
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四物汤对血虚证小鼠骨髓细胞CD34抗原表达的影响 被引量:20
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作者 谭洪玲 马增春 +3 位作者 高月 刘永学 肖成荣 路晓钦 《中药新药与临床药理》 CAS CSCD 2002年第1期11-13,共3页
目的探讨四物汤对60 Coγ射线或环磷酰胺诱导的血虚证小鼠骨髓细胞CD 34抗原分子表达的影响。方法用60 Coγ射线全身照射小鼠或腹腔注射环磷酰胺造成小鼠血虚证模型 ,实验分正常组、模型组、小剂量组、中剂量组、大剂量组 ,荧光标记小... 目的探讨四物汤对60 Coγ射线或环磷酰胺诱导的血虚证小鼠骨髓细胞CD 34抗原分子表达的影响。方法用60 Coγ射线全身照射小鼠或腹腔注射环磷酰胺造成小鼠血虚证模型 ,实验分正常组、模型组、小剂量组、中剂量组、大剂量组 ,荧光标记小鼠骨髓细胞并用流式细胞仪测定CD 34+ 细胞在骨髓有核细胞中的比例 ,同时做小鼠骨髓细胞集落培养。结果与结论四物汤可促进血虚小鼠骨髓中干祖细胞增生 ,增加CD 34抗原分子的表达 。 展开更多
关键词 四物汤 药理学 血虚 中医药疗法 骨髓细胞cd34抗原 疾病模型 中药
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幼年特发性关节炎患儿外周血自然杀伤细胞CD^(16+56+)的随访意义 被引量:3
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作者 姚翠婵 曾华松 +2 位作者 韦茹 王蓓 熊小燕 《实用医学杂志》 CAS 北大核心 2009年第11期1764-1766,共3页
目的:探讨幼年特发性关节炎全身型(systemic onset juvenile idiopathic arthritis,SOJIA)患儿治疗过程自然杀伤细胞(natural killer cells,NKcells)CD16+56+比率、骨髓细胞形态学等指标的动态变化在提示病情发展、改善预后方面的意义... 目的:探讨幼年特发性关节炎全身型(systemic onset juvenile idiopathic arthritis,SOJIA)患儿治疗过程自然杀伤细胞(natural killer cells,NKcells)CD16+56+比率、骨髓细胞形态学等指标的动态变化在提示病情发展、改善预后方面的意义。方法:对我院2006年6月至2008年6月收治的30例SOJIA病例,分析治疗早期及联合治疗后多项炎症指标、外周血NK cells CD16+56+比率、骨髓细胞形态学动态变化与临床表现之间的关系。结果:所有患儿中10例病情反复难治者,均出现非特异性炎症指标升高,9例外周血NK cells CD16+56+比率降低,8例骨髓细胞形态学发生变化(4例发现异形淋巴细胞,4例有吞噬现象)。及时采用强联合治疗方案后,8例病情得到控制,2例进展为巨噬细胞活化综合征。结论:SOJIA临床表现多样,实验室指标非特异性指向,治疗中同时警惕NK cells CD16+56+比率和骨髓细胞形态学等变化,及早采用个体化强联合治疗方案,是阻止病情发展,改善预后的关键。 展开更多
关键词 关节炎 幼年型类风湿 自然杀伤细胞cd16+56+ 骨髓细胞形态学 巨噬细胞活化综合征
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生长抑素对白血病患者骨髓细胞CD_(34)抗原表达的影响 被引量:1
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作者 张圣明 陈红霞 +4 位作者 于民海 姜红心 吕娥 于志刚 冉学红 《潍坊医学院学报》 2003年第2期90-92,T001,共4页
目的 研究生长抑素八肽类似物奥曲肽 (Octreotide ,SMS ;商品名 :善得定 )对白血病患者骨髓单个核细胞CD3 4 抗原表达的影响 ,探讨生长抑素对白血病细胞的调控作用。方法 采用细胞培养、免疫细胞化学技术及图像分析系统 ,随机计数CD3... 目的 研究生长抑素八肽类似物奥曲肽 (Octreotide ,SMS ;商品名 :善得定 )对白血病患者骨髓单个核细胞CD3 4 抗原表达的影响 ,探讨生长抑素对白血病细胞的调控作用。方法 采用细胞培养、免疫细胞化学技术及图像分析系统 ,随机计数CD3 4 阳性细胞数 ,观察分析SMS在白血病患者骨髓单个核细胞中CD3 4 抗原表达的分布和含量。结果 在相差倒置显微镜下 ,加入SMS组与对照组比较 ,细胞碎片增多 ,基质细胞减少 ;CD3 4 抗原主要表达于细胞膜和核膜 ;SMS组阳性细胞数显著少于对照组 (P <0 .0 1) ;图像分析光密度值 (OD值 )SMS组明显低于对照组 (P <0 .0 1)。结论 在体外SMS可减少造血干 祖细胞特异抗原 (CD3 4 )的表达。 展开更多
关键词 生长抑素 白血病 cd34抗原 骨髓细胞 奥曲肽 细胞培养 免疫细胞化学
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CD4^+CD25^+免疫调节性T细胞对CD4^+T细胞介导的移植物抗宿主病预防作用的研究 被引量:6
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作者 韩梅 王冠军 《中国免疫学杂志》 CAS CSCD 北大核心 2006年第4期323-326,共4页
目的:研究CD4+CD25+免疫调节性T(Treg)细胞在小鼠骨髓移植后,对移植物抗宿主病的预防作用及其作用机制。方法:用C3H(H2k)小鼠骨髓作为供体,提取C3H(H2k)小鼠CD4+T及CD4+CD25+T细胞,C3H×B6(H2k/b)F1小鼠为骨髓移植的受者。在受者接... 目的:研究CD4+CD25+免疫调节性T(Treg)细胞在小鼠骨髓移植后,对移植物抗宿主病的预防作用及其作用机制。方法:用C3H(H2k)小鼠骨髓作为供体,提取C3H(H2k)小鼠CD4+T及CD4+CD25+T细胞,C3H×B6(H2k/b)F1小鼠为骨髓移植的受者。在受者接受致死量全身放射后,输注供者去除T细胞的骨髓(ATBM),使其造血功能重建(ATBM组)。于不同的实验组给予CD4+(CD4组)T细胞,CD4+CD25+T(CD25组)或二者同时输注(CD4/CD25组)。观察各组小鼠移植物抗宿主病(GVHD)的发生率。结果:所有10只ATBM组小鼠至骨髓移植后60天仍全部存活,无GVHD发生;所有10只CD4组小鼠在骨髓移植10天内全部死于GVHD(P<0.01);所有5只CD25组小鼠于骨髓移植后60天仍全部存活,无明显GVHD发生(P>0.05);同样,所有6只CD4/CD25组小鼠至骨髓移植后仍全部存活,无明显GVHD发生(P>0.05)。结论:在同种异基因小鼠的骨髓移植模型中,CD4+CD25+T不诱导GVHD的发生,并有预防CD4+T细胞介导的GVHD发生的作用。 展开更多
关键词 ^cd4^+cd25^+TREG 骨髓移植 GVHD
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Impact of T cells on hematopoietic stem and progenitor cell function:Good guys or bad guys?
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作者 Sulima Geerman Martijn A Nolte 《World Journal of Stem Cells》 SCIE CAS 2017年第2期37-44,共8页
When hematopoietic stem and progenitor cells(HSPC)are harvested for transplantation, either from the bone marrow or from mobilized blood, the graft contains a significant number of T cells. It is these T cells that ar... When hematopoietic stem and progenitor cells(HSPC)are harvested for transplantation, either from the bone marrow or from mobilized blood, the graft contains a significant number of T cells. It is these T cells that are the major drivers of graft-vs-host disease(Gv HD). The risk for Gv HD can simply be reduced by the removal of these T cells from the graft. However, this is not always desirable, as this procedure also decreases the engraftment of the transplanted HSPCs and, if applicable, a graft-vs-tumor effect. This poses an important conundrum in the field: T cells act as a double-edged sword upon allogeneic HSPC transplantation, as they support engraftment of HSPCs and provide anti-tumor activity, but can also cause Gv HD. It has recently been suggested that T cells also enhance the engraftment of autologous HSPCs, thus supporting the notion that T cells and HSPCs have an important functional interaction that is highly beneficial, in particular during transplantation. The underlying reason on why and how T cells contribute to HSPC engraftment is still poorly understood. Therefore, we evaluate in this review the studies that have examined the role of T cells during HSPC transplantation and the possible mechanisms involved in their supporting function. Understanding the underlying cellular and molecular mechanisms can provide new insight into improving HSPC engraftment and thus lower the number of HSPCs required during transplantation. Moreover, it could provide new avenues to limit the development of severe Gv HD, thus making HSPC transplantations more efficient and ultimately safer. 展开更多
关键词 HEMATOPOIETIC STEM cells HEMATOPOIETIC STEM and PROGENITOR cells cd8 T cells Transplantation ENGRAFTMENT Memory T cells Facilitating cells bone marrow
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