期刊文献+
共找到15篇文章
< 1 >
每页显示 20 50 100
Analysis of CD4^+CD25^+ Regulatory T Cells and Foxp3 mRNA in the Peripheral Blood of Patients with Asthma 被引量:15
1
作者 薛克营 周咏明 +2 位作者 熊盛道 熊维宁 唐滔 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第1期31-33,共3页
The changes of CD4^+CD25^+ regulatory T cells (CD4^+CD25^+ Treg) and Foxp3 mRNA in peripheral blood mononuclear cells (PBMCs) from patients with asthma were investigated in order to elucidate the possible role... The changes of CD4^+CD25^+ regulatory T cells (CD4^+CD25^+ Treg) and Foxp3 mRNA in peripheral blood mononuclear cells (PBMCs) from patients with asthma were investigated in order to elucidate the possible roles of CD4^+CD25^+ Treg in the development of asthma. The peripheral blood samples were collected from 29 healthy controls (normal control group) and 78 patients with asthma which included 30 patients in exacerbation group, 25 patients in persistent group, and 23 patients in remission group. By using flow cytometry and RT-PCR, the CD4^+CD25^+ Treg ratio and Foxp3 mRNA in PBMCs were detected. The CD4^+CD25^+ Treg ratio and Foxp3 mRNA in PBMCs of exacerbation and persistent groups were lower than that of remission and normal control groups (P〈0.05). Although the CD4^+CD25^+ Treg ratio and Foxp3 mRNA of remission group were also lower than that of normal control group, there was no significant difference between them (P〉0.05). As compared with persistent group, exacerbation group had lower CD4^+CD25^+ Treg ratio and Foxp3 mRNA (P〈0.05). It was indicated that the decrease of CD4^+CD25^+ Treg ratio and its function in PBMCs may be responsible for pathogenesis of asthma. 展开更多
关键词 AStHMA peripheral blood mononuclear cells cd4^+cd25^+ regulatory t cells Foxp3 mRNA
下载PDF
Depletion of CD4^+CD25^+ regulatory T cells can promote local immunity to suppress tumor growth in benzo[a]pyrene-induced forestomach carcinoma 被引量:9
2
作者 Yi-Ling Chen Jung-Hua Fang +1 位作者 Ming-Derg Lai Yan-Shen Shan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第38期5797-5809,共13页
AIM: To elucidate the distribution of CD4^+CD25^+ regulatory T cells (Tregs) in different lymphoid tissues and its local enhancement on tumor growth before and after depletion of CD4^+CD25^+ Tregs. METHODS: Fe... AIM: To elucidate the distribution of CD4^+CD25^+ regulatory T cells (Tregs) in different lymphoid tissues and its local enhancement on tumor growth before and after depletion of CD4^+CD25^+ Tregs. METHODS: Female ICR mice were garaged with benzo[a]pyrene (BaP) to induce forestomach carcinoma. CD4^+CD25^+ Tregs were intraperitoneally depleted with monoclonal antibody PC61. These mice were divided into BaP-only, BaP + IgG, BaP + PC61, and control groups. The forestomach of mice was dissected for histological analysis, and tunnel test was performed for apoptosis of tumor cells. CD4^+CD25^+ Tregs were sorted from different lymphoid tissues and expression of Foxp3, IL-10, and chemokine receptors was analyzed by flow cytometry, semi-quantitative and veal-time polymerase chain reaction. RESULTS: The mice gavaged with only BaP showed increased forestomach papilloma and carcinoma at wk 16 and 32. The proportion of CD4^+CD25^+ Tregs was significantly higher in peri-stomach regional lymph nodes than in other lymphoid tissues. These CD4^+CD25^+ Tregs in regional lymph nodes expressed higher levels of Foxp3 and IL-10, enriched in the CD62L-subset, and CCR1 and CCR5 chemokine receptors. In mice gavaged with BaP + PC61, the number of tumor nodules and tumor volume decreased significantly with massive infiltrating cells and apoptosis of tumor cells. In the draining regional lymph nodes, the number of CD4^+CD25^+ Tregs also decreased significantly. CONCLUSION: Inducible and activated CD4^+CD25^+ Tregs in the draining regional lymph nodes suppress host local immunity during tumor growth. Depletion of CD4^+CD25^+ Tregs can promote host local immunity to suppress tumor growth. 展开更多
关键词 cd4^+cd25^+ regulatory t cells Forestomach tumor FOXP3
下载PDF
Influence of Danshen Injection on airway inflammation and CD4^+ CD25^+ regulatory T cells of asthmatic rats 被引量:6
3
作者 Keying Xue Yongming Zhou +2 位作者 Shengdao Xiong Weining Xiong Dan Li 《Journal of Nanjing Medical University》 2006年第5期292-295,共4页
Objective: To investigate the influence of Danshen Injection on airway inflammation and CD4^+CD25^+ regulatory T cells(CD4^+CD25^+ Tr) of asthmatic rats, and elucidate the possible mechanism of Danshen Inject... Objective: To investigate the influence of Danshen Injection on airway inflammation and CD4^+CD25^+ regulatory T cells(CD4^+CD25^+ Tr) of asthmatic rats, and elucidate the possible mechanism of Danshen Injection in treatment of asthma. Methods: 30 Wister rats were randomly divided into control group, asthma group and Danshen Injection treated group. Bronchoalveolar lavage fluids (BALF) were collected, and cytology studies were conducted. Lung tissues were obtained and pathologic analyses were done with hematoxylin and eosin stain (HE). Flow cytometry was used to detect the CD4^+CD25^+ Tr ratio in peripheral blood mononuclear cells (PBMCs). Results: Total cell, the percentage of lymphocytes, neutrophils and eosinophils (Eos) in BALF of Danshen Injection-treated group were lower than that in asthma group (P〈0.05, P〈0.01). Compared with asthma group, less infiltration of inflammatory cells in lung tissues was observed in Danshen Injection-treated group. CD4^+CD25^+ Tr of asthma group was lower than that of control and Danshen Injection treated group (P〈0.05). Conclusion: Danshen Injection can suppress airway inflammation of asthmatic rats, probably by increasing the number of CD4^+CD25^+ Tr. 展开更多
关键词 Danshen Injection AStHMA airway inflammation cd4^+cd25^+ regulatory t cells
下载PDF
Isolation and identification of CD4^+CD25^+ regulatory T cells in rat 被引量:1
4
作者 Ling Lü Feng Zhang Liyong Pu Chao Jiang 《Journal of Nanjing Medical University》 2006年第4期238-241,共4页
Objective: To establish a stable and high efficient method for collection of CD4^+CD25^+ regulatory T cells from rats in vitro. Methods: CD4^+CD25^+ regulatory T cells were isolated from the rat splenic cells th... Objective: To establish a stable and high efficient method for collection of CD4^+CD25^+ regulatory T cells from rats in vitro. Methods: CD4^+CD25^+ regulatory T cells were isolated from the rat splenic cells through two steps by magic cell sorting (MACS) system. The first step was negative selection of CD4^+T cells by cocktail antibodies and anti-IgG magic microbeads, and the second step was positive selection of CD25^+T cells by anti-CD25 PE and anti-PE magic microbeads. The purity and viability of separated cells were measured by flow cytometry (FACS) and Trypan blue staining. The suppressive ability of seperated cells on the proliferation of CD4^+CD25^- T cells was assessed by cell proliferation assay. Results: The purity of negatively enriched CD4^+ T cells was 79%-87% (83.6%±2.5% ) , and the purity of positively enriched CD4^+CD25^+ T cells was 86%- 93% ( 90.2±1.8% ) with the viability of 92%~95% (92.8% ± 3.4% ). The enriched cells significantly suppressed the proliferation of CD4^+CD25^- T cells in mixed lymphocyte culture (P 〈 0.05). Conclusion: An effective method can be established for enrichment of CD4^+CD25^+ regulatory T cells in two steps by MACS, with satisfied cell purity, viability and function. 展开更多
关键词 magic cell sorting system cd4^+cd25^+ regulatory t cells flow cytometry technique RAtS
下载PDF
Effect of CD4^+CD25^+ regulatory T cells in the development of anterior chamber-associated immune deviation
5
作者 Shu-Xing Ji, Pei-Zeng Yang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2011年第1期19-25,共7页
AIM: To investigate whether CD4(+)CD25(+) regulatory T (Treg) cells play a role in the development of anterior chamber-associated immune deviation (ACAID). METHODS: The dynamic changes in the frequency of CD4(+)D25(+)... AIM: To investigate whether CD4(+)CD25(+) regulatory T (Treg) cells play a role in the development of anterior chamber-associated immune deviation (ACAID). METHODS: The dynamic changes in the frequency of CD4(+)D25(+) T cells, CD4(+)D25(+) FoxP3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells from spleens of mice with ACAID were analyzed by flow cytometry. Foxp3 mRNA expression in purified CD4(+)CD25(+) T cells was analyzed using real-time PCR. The suppressive effect of purified CD4(+)CD25(+) T cells on the proliferation of CD4(+)CD25(-) T cells was evaluated by [H-3] thymidine incorporation. A blocking experiment was performed to further address the role of CD4(+)CD25(+) T cells in ACAID. The expression of IL-10 in purified CD4(+)CD25(+) T cells was evaluated by ELISA. RESULTS: Increased frequencies of CD4(+)CD25(+) T cells, CD4(+)CD25(+) Foxp3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells were observed in ACAID. The CD4(+)CD25(+) T cells from mice with ACAID showed enhanced suppressive effect on the proliferation of CD4(+)CD25(-) T cells. Treatment of BALB/c mice with anti-CD25 antibody after injection of OVA into the anterior chamber significantly inhibited the induction of ACAID. Furthermore, purified CD4(+)CD25(+) T cells from ACAID mice secreted IL-10. CONCLUSION: Our results demonstrate that Treg cells are induced in the mice undergoing ACAID. These Treg cells may play a role in the development of ACAID. 展开更多
关键词 cd4+cd25+ regulatory t cells FOXP3 ACAID IL-10 PD-1
下载PDF
CD4^+CD25^(high) Regulatory Cells in Peripheral Blood of NSCLC Patients
6
作者 刘莉 姚军霞 +1 位作者 丁乾 黄士昂 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第5期548-551,共4页
The proportion and changes of CD4^+CD25^high regulatory T cells (Trs) in peripheral blood of non-small cell lung cancer (NSCLC) patients were analyzed and their clinical significance explored. The peripheral bloo... The proportion and changes of CD4^+CD25^high regulatory T cells (Trs) in peripheral blood of non-small cell lung cancer (NSCLC) patients were analyzed and their clinical significance explored. The peripheral blood was collected from 61 patients with NSCLC and 15 healthy controls. By using monoclonal antibodies, the blood samples were evaluated with the flow cytometry for lymphocyte subsets (CD3^+, CD4^+ and CD8^+) and CD4^+CD25^high Tr cells. The results showed that the proportion of CD4^+CD25^high Tr cells in NSCLC group was significantly higher than in control group [(4.36 ±2.07) % vs (2.04±1.03) %, P〈0.01]. The proportion of CD4^+CD25^ high Tr cells in late stage was higher than that in early stage [stages Ⅰ +Ⅱ (2.264±0.6) %; stage Ⅲ(3.284± 1.38) %; stage IV (6.06 4±4.08) %] (P〈0.05). Kaplan-Meier survival analysis revealed that the prognosis of the patients who had higher proportion of CD4^+CD25^high Tr cells in peripheral blood was worse (P=0.0026). In conclusion, the relative increase in CD4^+CD25^high Tr cells in peripheral blood may be related to im- munosuppression and tumor progression in patients with NSCLC. This finding suggests that CD4^+CD25^high Tr cells in peripheral blood of NSCLC may be positive for prognosis analysis. The use of depletion of the CD4^+CD25^high Tr cell therapy to treat NSCLC patients may be an effective strategy. 展开更多
关键词 non-small cell lung cancer t subsets cd4^+cd25^high regulatory t cell flow cytometry survival analysis
下载PDF
Prognostic value of CD4^+CD25^+ Tregs as a valuable biomarker for patients with sepsis in ICU 被引量:19
7
作者 Kun Chen Qiu-xiang Zhou +2 位作者 Hong-wei Shan Wen-fang Li Zhao-fen Lin 《World Journal of Emergency Medicine》 CAS 2015年第1期40-43,共4页
BACKGROUND: Sepsis is a common complication ofinfections, burns, traumas, surgeries, poisonings, and post-cardiopulmonary resuscitation. The present study aimed to investigate prognostic value of CD4+CD25+ regulatory ... BACKGROUND: Sepsis is a common complication ofinfections, burns, traumas, surgeries, poisonings, and post-cardiopulmonary resuscitation. The present study aimed to investigate prognostic value of CD4+CD25+ regulatory T cells(Treg) in peripheral blood of patients with sepsis.METHODS: Periphery blood from 28 patients diagnosed with sepsis was collected on day 1 and 7 after hospitalization in the ICU of Shanghai Changzheng Hospital between December 2013 to April 2014. The blood was used for analyses of Treg ratio using flow cytometry and for analyses of blood routine test, C-reactive protein(CRP), bilirubin, procalcitonin(PCT), and coagulation. APACHE II and sequential organ failure assessment(SOFA) scores were also investigated. The results were compared between two outcome groups of survival or death to evaluate prognostic value for sepsis.RESULTS: The patients had an average age of 60.36±15.03 years, APACHE II score 16.68±7.00, and SOFA score 7.18±3.78. Among the 28 patients, 12 had severe trauma(42.9%), 10 had septic shock(35.7%), and 9(32.2%) died. The median ratio of Tregs was 2.10%(0.80%, 3.10%) in the survival group vs. 1.80%(1.15%, 3.65%) in the death group(Z=–0.148, P=0.883) on day 1; however it was signifi cantly changed to 0.90%(0.30%, 2.80%) vs. 5.70%(2.60%, 8.30%)(Z=–2.905, P=0.004).CONCLUSION: With better prospects for clinical application, dynamic monitoring of Tregs ratio in peripheral blood has potential value in predicting prognosis of sepsis. 展开更多
关键词 cd4+cd25+ regulatory t cells SEPSIS Prognosis BIOMARKER APACHE SOFA PROCALCItONIN
下载PDF
INVESTIGATION OF REGULATORY T CELLS IN PATIENTS WITH NON-SMALL CELL LUNG CANCER
8
作者 张路 梁永杰 +3 位作者 任涛 朱辉 徐志龙 戴亚蕾 《Medical Bulletin of Shanghai Jiaotong University》 CAS 2010年第1期6-9,共4页
Objective To evaluate the prevalence of CD4 ^+ CD25^high regulatory T cells ( Treg cells) in the peripheral blood mononuclear cells (PBMC) and tumor-infiltrating lymphocytes (TIL) of patients with non-small cel... Objective To evaluate the prevalence of CD4 ^+ CD25^high regulatory T cells ( Treg cells) in the peripheral blood mononuclear cells (PBMC) and tumor-infiltrating lymphocytes (TIL) of patients with non-small cell lung cancer (NSCLC) and to investigate immunosuppression to the progression of cancer. Methods Peripheral blood and tumor tissues were collected from 20 patients with NSCLC at the time of surgery. None of the patients received surgery, radiotherapy, chemotherapy, or other medical interventions before this study. Cancer stages of the patients were Ⅰ-Ⅲ A. Venous blood samples were obtained from 20 health donors. PBMC were isolated from blood samples by differential centrifugation over Ficoll-Hypaque. TILs were isolated from tumors by differential centrifugation over Ficoll-Hypaque and Percoll. Percentage of CD4^+ CD25^highTr/CD4+T in PBMC and TIL was assessed by the flow cytometry. Results The percentage of CD4^ + CD25high Tr/ CD4 ^+T in PBMC [ (4. 87 ± 1.22 ) % ] of NSCLC patients was significantly higher than that in healthy donors [ ( 2.36 ± 0. 72 ) % ] ( P 〈 0.01 ). The percentage of CD4^+ CD25^highTr/ CD4^+ T in PBMC [ (5.40 ± 1.20) % ] of NSCLC patients in stage Ⅱ-Ⅲ A was significantly higher than that in stage Ⅰ [ (3. 87 ± 0. 22 ) % ] ( P 〈 0. 01 ). The percentage of CD4 + CD25hiShTr/ CD4 + T in TIL[ ( 8. 66 ±0. 76) % ] of NSCLC patients in stage Ⅱ-Ⅲ A was significantly higher than that in stage Ⅰ [ ( 7. 04 ± 0. 80) % ] ( P 〈 0. 01 ). Conclusion The prevalence of CD4 ^+ CD25^highTreg cells in PBMC and TIL of NSCLC patients was significantly higher than that in healthy donors. These Treg cells may be preventing appropriate antitumor immune responses. The population of CD4^ + CD25^highTreg cells in PBMC and TILs of NSCLC patients with Ⅱ-Ⅲ A stage was significantly higher than that of NSCLC patients with Ⅰ stage. These Treg cells may facilitate development of tumors. 展开更多
关键词 non-small cell lung cancer cd4 ^+ cd25^high regulatory t cells tumor-infiltrating lymphocyte
下载PDF
Hepatocellular carcinoma and macrophage interaction induced tumor immunosuppressionvia Treg requires TLR4 signaling 被引量:20
9
作者 Jing Yang Jin-Xiang Zhang +3 位作者 Hui Wang Guo-Liang Wang Qing-Gang Hu Qi-Chang Zheng 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第23期2938-2947,共10页
AIM: To investigated the interaction between toll-like receptor 4 (TLR4)-activated hepatoma cells and macrophages in the induction of tumor-immune suppression mediated by CD4+CD25high family of transcription factor P3... AIM: To investigated the interaction between toll-like receptor 4 (TLR4)-activated hepatoma cells and macrophages in the induction of tumor-immune suppression mediated by CD4+CD25high family of transcription factor P3 (FOXP3) regulatory T cells (Tregs). METHODS: The proportion of FOXP3+ Tregs was identified in peripheral blood and tumor tissues of 60 hepatocellular carcinoma (HCC) patients. TLR4 expression was examined in tumor tissues and cell lines. The correlation was examined between FOXP3+ Tregs in peripheral blood and TLR4 expression of HCC tissues. Following activation of TLR4 in H22 murine hepatoma cells pre-incubated with lipopolysaccharide (LPS) and co-cultured with macrophage cell line RAW246.7, the synthesis of cytokines tumor necrosis factor-α, CCL22, and interleukin (IL)-10 by the two cell lines was detected and analyzed. RESULTS: FOXP3+ Tregs were enriched in tumor sites, and circulating FOXP3+ Tregs were increased in HCC patients in correlation with multiple tumor foci and up-regulated TLR4 expression in HCC tissues. Semi-quantitative analysis indicated that TLR4 was over-expressed in HCC compared with the matched normal tissues. Cell cultivation experiments indicated that the mRNAs of IL-10 and CCL22 were significantly up-regulated in the RAW246.7 cell line when co-cultured with LPS preincubated H22 cells. CONCLUSION: In hepatoma cell lines, TLR4 may indirectly facilitate the recruitment of Tregs to the tumor site and promote intrahepatic metastasis through its interaction with macrophages. 展开更多
关键词 cd4cd25^high FOXP3+ regulatory t cell toll-like receptor tumor immunity Hepatocellular car-cinoma MACROPHAGE
下载PDF
Hepatitis C virus core protein triggers expansion and activation of CD4+CD25+ regulatory T cells in chronic hepatitis C patients 被引量:10
10
作者 Naicui Zhai Xiumei Chi +7 位作者 Tianyang Li Hongxiao Song Haijun Li Xia Jin Ian Nicholas Crispe Lishan Su Junqi Niu Zhengkun Tu 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2015年第6期743-749,共7页
CD4+CD25+FoxP3 + regulatory T ceils (Tregs) are increased in patients with chronic hepatitis C, which may contribute to the sustained suppression of hepatitis C virus (HCV)-specific T-cell responses and viral p... CD4+CD25+FoxP3 + regulatory T ceils (Tregs) are increased in patients with chronic hepatitis C, which may contribute to the sustained suppression of hepatitis C virus (HCV)-specific T-cell responses and viral persistence in HCV-infected individuals. We postulated that HCV core protein (HCVc) directly contributes to the expansion of Tregs in HCV-infected patients, and we provide evidence to support this hypothesis in the report. Peripheral blood mononuclear cells (PBMCs) and sera were collected from 87 treatment-naive chronic HCV-infected patients, CD4+CD25+ Tregs were measured by flow cytometry, and HCV RNA and HCVc levels were detected using qPCR and enzyme-linked immunosorbent assay (ELISA), respectively. CD4+, CD8+, CD4+CD25+ and CD4+CD25- T cells were purified from healthy donors and cultured with recombinant HCVc and Toll-like receptor (TLR) ligands. Flow cytometry was used to analyze cell proliferation, and ELISA was performed to measure cytokine production. In the 87 chronic HCV-infected patients, HCVc showed a significant correlation with HCV RNA and CD4+CD25+Tregs. Mechanistic studies showed that HCVc, together with anti-CD3 antibody, augmented CD4+CD25+ Treg proliferation, but inhibited CD4+CD25- T-cell proliferation and IFN-γ production, in a dose-dependent and Treg-dependent manner. Moreover, unlike the TLR3 ligand (poly hC) and the TLR4 ligand (lipopolysaccharide, LPS), the TLR2 ligand (lipoteichoic acid, LTA) and HCVc both inhibited TCR-induced CD4+ T-cell proliferation and IFN-γ secretion in a Treg-dependent manner. These data indicate that HCVc, like other TLR2 ligands, triggers CD4+CD25+ Treg activation and expansion to inhibit host immune responses, which may play a critical role in viral persistence in HCV-infected patients. 展开更多
关键词 cd4cd25 regulatory t cells HCV core toll-like receptor
原文传递
Alterations of peripheral CD4^(+)CD25^(+)Foxp3^(+)T regulatory cells in mice with STZ-induced diabetes 被引量:4
11
作者 Yu Zhen Lina Sun +7 位作者 He Liu Kaizhong Duan Chun Zeng Lianjun Zhang Di Jin Jianxia Peng Wenjun Ding Yong Zhao 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2012年第1期75-85,共11页
Complications arising from abnormal immune responses are the major causes of mortality and morbidity in diabetic patients.CD4^(+)CD25^(+)T regulatory cells(Tregs)play pivotal roles in controlling immune homeostasis,im... Complications arising from abnormal immune responses are the major causes of mortality and morbidity in diabetic patients.CD4^(+)CD25^(+)T regulatory cells(Tregs)play pivotal roles in controlling immune homeostasis,immunity and tolerance.The effect of hyperglycemia on CD4^(+)CD25^(+)Tregs has not yet been addressed.Here we used streptozotocin(STZ)-induced diabetic mice to study the effects of long-term hyperglycemia on CD4^(+)CD25^(+)Tregs in vivo.Four months after the onset of diabetes,the frequency of CD4^(+)CD25^(+)Foxp3^(+)T regulatory cells was significantly elevated in the spleen,peripheral blood lymphocytes(PBLs),peripheral lymph nodes(pLNs)and mesenteric LNs(mLNs).CD4^(+)CD25^(+)Tregs obtained from mice with diabetes displayed defective immunosuppressive functions and an activated/memory phenotype.Insulin administration rescued these changes in the CD4^(+)CD25^(+)Tregs of diabetic mice.The percentage of thymic CD4^(+)CD25^(+)naturally occurring Tregs(nTregs)and peripheral CD41Helios1Foxp31 nTregs were markedly enhanced in diabetic mice,indicating that thymic output contributed to the increased frequency of peripheral CD4^(+)CD25^(+)Tregs in diabetic mice.In an in vitro assay in which Tregs were induced fromCD4^(+)CD25^(+)T cells by transforming growth factor(TGF)-b,high glucose enhanced the efficiency of CD4^(+)CD25^(+)Foxp3^(+)T inducible Tregs(iTregs)induction.In addition,CD4^(+)CD25^(+)T cells from diabetic mice were more susceptible to CD4^(+)CD25^(+)Foxp3^(+)TiTreg differentiation than those cells from control mice.These data,together with the enhanced frequency of CD4^(+)CD25^(+)Foxp3^(+)T iTregs in the periphery of mice with diabetes,indicate that enhanced CD4^(+)CD25^(+)Foxp3^(+)T iTreg induction also contributes to a peripheral increase in CD4^(+)CD25^(+)Tregs in diabetic mice.Our data show that hyperglycemia may alter the frequency of CD4^(+)CD25^(+)Foxp3^(+)T Tregs in mice,which may result in late-state immune dysfunction in patients with diabetes. 展开更多
关键词 cd4^(+)cd25^(+)Foxp3^(+)t regulatory t cells DIABEtES HYPERGLYCEMIA immune disorder MICE
原文传递
Viral infection parameters not nucleoside analogue itself correlates with host immunity in nucleoside analogue therapy for chronic hepatitis B 被引量:16
12
作者 Cheng-Zhong Li Jing-Jing Hu +5 位作者 Jian-Ya Xue Wei Yin Ya-Yun Liu Wen-Han Fan Hao Xu Xue-Song Liang 《World Journal of Gastroenterology》 SCIE CAS 2014年第28期9486-9496,共11页
AIM: To determine the relationship between host immunity and the characteristics of viral infection or nucleoside analogues (NAs) themselves in patients with chronic hepatitis B (CHB) receiving NA therapy.
关键词 Chronic hepatitis B Nucleoside analogues Immune modulation Programmed death-1 cd4+cd25+FoxP3+t regulatory cells
下载PDF
Biological features of intrahepatic CD4^(+)CD25^(+)T cells in the naturally tolerance of rat liver transplantation
13
作者 LU Ling ZHANG Feng +5 位作者 PU Liyong YAO Aihua YU Yue SUN Beicheng LI Guoqiang WANG Xuehao 《Frontiers of Medicine》 SCIE CSCD 2007年第4期373-376,共4页
The biological features of intrahepatic CD4^(+)CD25^(+)T regulatory cells in the naturally tolerance of rat liver transplantation were explored.Orthotopic liver transplan-tation was performed in two allogeneic rat str... The biological features of intrahepatic CD4^(+)CD25^(+)T regulatory cells in the naturally tolerance of rat liver transplantation were explored.Orthotopic liver transplan-tation was performed in two allogeneic rat strain combina-tions,one with fatal immunosuppression despite a complete major histocompatibility complex mismatch.The subjects were divided into three groups according to different donors and recipients[Tolerance group:LEW-to-DA;Rejection group:DA-to-LEW;Syngegnic group(control group):DA-to-DA].The proportion of intrahepatic CD4^(+)CD25^(+)T cells from three groups was determined by flow cytometry(FCM)in different time.The intrahepaitc CD4^(+)CD25^(+)T cells were isolated by magnetic activated cell sorting(MACS)method and identified by FCM.The Foxp3 mRNA was detected by reverse transcriptase polymerase chain reaction(RT-PCR).And their suppression on the proliferation of CD4^(+)CD25^(-)T effector cells was analyzed by cell proliferation assay in vitro.Beginning immediately after transplantation,the proportion of Treg cells increased over time in both allogeneic groups but was significantly greater in the Rejection group.The pro-portion of Treg cells declined after day 5,and such reduction was more dramatic in the Rejection group than in the Tole-rance group.Animals in the Tolerance group showed a second increase in the proportion after day 14.Intrahepatic CD4^(+)CD25^(+)T cells isolated from spontaneous tolerance models inhibited the proliferation of mixed lymphocyte reaction.The purity of CD4^(+)CD25^(+)Tcells sorted by MACS was 86%–93%.The CD4^(+)CD25^(+)T cells could specifically express the Foxp3 gene compared with CD4^(+)CD25^(-)T cells.In vitro,the spleen cells from LEW rats can irritate the proliferation of CD4^(+)CD25^(+)T cells more obviously than the syngegnic spleen cells.CD4^(+)CD25^(+)Tr cells could suppress the proliferation of CD4^(+)CD25^(-)T cells,but the inhibition was reversed by exo-genous IL-2(200 U/mL).The CD4^(+)CD25^(+)T regulatory cells specifically express the Foxp3 gene,which may play animpor-tant role in the induction of liver transplantation tolerance by suppressing the reaction of effective T cells. 展开更多
关键词 liver transplantation immune tolerance t-lymphocytes regulatory cd4^(+)cd25^(+)t regulatory cells
原文传递
Association of T regulatory cells with natural course and response to treatment with interferon-α in patients with chronic hepatitis B infection 被引量:10
14
作者 XU Hong-tao XING Tong-jing +1 位作者 LI Hao YE Jun 《Chinese Medical Journal》 SCIE CAS CSCD 2012年第8期1465-1468,共4页
Background Regulatory T cell populations, particularly CD4+CD25+ T regulatory cells, have been implicated in the persistence of hepatitis B virus (HBV) infection. However, no clear relationship has been establishe... Background Regulatory T cell populations, particularly CD4+CD25+ T regulatory cells, have been implicated in the persistence of hepatitis B virus (HBV) infection. However, no clear relationship has been established between the frequency of CD4~CD25~ T regulatory cells in the peripheral blood and either the disease phases in the natural history of chronic HBV infection or in the response to interferon-a therapy. Methods In the present study, three different common markers of CD4+CD25+ T regulatory cells were used to determine the numbers of T regulatory cells in healthy controls and in patients with chronic HBV infection. Results No significant difference was found when samples were gated for CD25hi and CD25+FoxP3+ T cells. A significant correlation was found between the number of CD4+ Treg cells that gated with CD25+FoxP3+ and CD25+CD127low/- in healthy controls and in patients with chronic hepatitis B (CHB) (r=0.67, 0.59; P 〈0.01). The percentages of Treg cells were (8.56±2.01)% in asymptomatic carriers (Asc), (8.74±3.04)% in inactive HBsAg carriers, (10.7±2.93)% in CHB and (7.42±1.28)% in healthy controls (F=-11.1, P 〈0.001). The percentage of Treg cells in patients with CHB was higher than in asymptomatic HBV patients, inactive HBsAg carriers, or healthy controls (P 〈0.01). The proportion of CD4+CD25+CD127 low/T cells in patients who responded to interferon-(] was (11.9±3.3)%, (9.1±2.4)% and (9.0±2.9)% at baseline, week 12 and week 24 after treatment, respectively (Z=2.42, P〈0.05; Z=2.67, P〈0.01). Conclusions These results suggest that the proportion of the CD4+CD25+ regulatory T cells might be affected by the application of different markers in process to detect T regulatory cells. The frequency of Treg cells was increased in patients with CHB, which might be associated with the disease activity of these patients and contribute to prevention of extensive liver damage. A decline in Treg cells at week 12 of treatment might be associated with a better response to treatment with interferon-α. 展开更多
关键词 cd4cd25 regulatory t cells FOXP3 chronic hepatitis B INtERFERON-Α
原文传递
Immune responses on allograft heart transplantation in inbred rats infected with Echinococcosis multilocularis 被引量:2
15
作者 Mai Hepiretihan.Ai Erken ZHAO Jin-ming +2 位作者 GUAN Xiao-yan WEN Hao WANG Yun-hai 《Chinese Medical Journal》 SCIE CAS CSCD 2012年第24期4412-4417,共6页
Background Alveolar echinococcosis (AE) is caused by the metacestode stage of Echinococcus multilocularis (E. multilocularis) and is a rare but life-threatening disease. This disease commonly is characterized by a... Background Alveolar echinococcosis (AE) is caused by the metacestode stage of Echinococcus multilocularis (E. multilocularis) and is a rare but life-threatening disease. This disease commonly is characterized by an infiltrative, tumor-like growth of the E. multilocularis metacestode in the liver of human. Liver transplantation is an effective therapy for end-stage of hepatic AE, but the characteristics of host immunity associated with E. multilocularis infection with organ transplantation are poorly defined. We hereby aimed to study the immunological status and allograft heart survival in inbred rats with E. multilocularis infection. Methods Rat models of AE were established by injecting the E. multilocularis suspension made from E. multilocularis infected tissues into the abdomen of Lewis (LEW) rats. Three months later, in the experimental group, allograft heart transplantation was performed from Brown-Norway (BN) rats to the E. multilocularis infected LEW rats. In the control group, we transplanted hearts from BN rats to healthy LEW rats. The influence of the disturbed immune system in E. multilocularis infected rats on the heart transplantation was assessed, including observation of allograft heart survival time, histopathological examination of grafts and immunohistochemical examination of infiltrating cells (CD4+ T cells, CD8+ T cells and eosinophile granulocytes), measurement of interleukin (IL)-4 and interferon (IFN)-γ in the serum by enzyme-linked immunosorbent assay (ELISA) and analysis of CD4+CD25+ regulatory T cells in peripheral blood by fluorescence activated cell sorting (FACS) flow cytometric analysis. Results The survival time of recipients in the experimental group was prolonged compared with those in the control group. The numbers of graft infiltrating CD8+ T cells were decreased whereas the graft infiltrating eosinophil granulocytes (CD15+) were increased in grafts in the experimental group (P 〈0.05). Furthermore, the proportion of CD4+CD25+regulatory T cells in the peripheral blood was 10.8% on average in the experimental group, which was significantly higher than that in the control group (6.1%). In addition, the level of serum IL-4 in E. multilocularis infected rats was higher than that in the control group rats, whereas the level of serum IFN-γ in experimental group was lower than that in the control group when graft rejection occurred (P 〈0.05). Conclusions This study suggests that E. multilocularis infection could prolong the allograft survival time through the polarization of Th1/Th2-type cells and induction of CD4+CD25+ regulatory T cells. This strategy may provide a new idea for establishing transplantation tolerance. 展开更多
关键词 immune responses heart transplantation ECHINOCOCCOSIS cd4 cd25 regulatory t cells allografi heart transplantation
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部