RhoA、CD4^(+)CD25^(+)调节性T细胞、MYBL2在胃癌患者中的表达及对预后和生存时间的影响

目的:探讨RhoA、CD4^(+)CD25^(+)调节性T细胞、成髓细胞瘤转录因子第2亚型(MYBL2)在胃癌患者中的表达及对预后和生存时间的影响。方法:选取2017年1月至2019年1月于本院就诊的134例胃癌患者术后癌组织标本作为研究对象,另选取其中62例胃癌患者相应的癌旁组织标本及40例同期非胃癌患者正常胃黏膜组织标本进行对照,检测癌组织、癌旁组织、正常胃黏膜组织中RhoA、CD4^(+)CD25^(+)调节性T细胞、MYBL2的表达情况,分析其对胃癌预后及生存时间的影响。结果:RhoA,CD4^(+)CD25^(+)调节性T细胞、MYBL2在癌组织中的阳性率均明显高于癌旁组织和正常胃黏膜组织(P<0.05)。胃癌患者术后平均生存时间为(28.61±1.34)个月,其中RhoA、CD4^(+)CD25^(+)调节性T细胞、MYBL2阳性患者的生存时间均短于阴性患者(P<0.05)。RhoA(+)、CD4^(+)CD25^(+)调节性T细胞(+)、MYBL2(+)是胃癌患者预后的危险因素(P<0.05)。结论:检测RhoA,CD4^(+)CD25^(+)调节性T细胞、MYBL2的表达可作为胃癌病情严重程度、预后及生存时间评估的重要补充手段。

外周血CD4^(+)CD25^(+)、CD8^(+)CD28^(+)调节性T细胞水平对早期宫颈癌患者腹腔镜根治术后预后的预测价值

目的分析外周血CD4^(+)CD25^(+)、CD8^(+)CD28^(+)调节性T细胞水平对早期宫颈癌(CC)患者腹腔镜根治术后预后的预测价值。方法招募2018年9月至2020年9月于儋州市人民医院接受腹腔镜根治术治疗的早期CC患者204例,根据患者术后随访期间预后情况分为预后不良组(43例)和预后良好组(161例)。比较两组临床资料。采用Spearman秩相关分析外周血CD4^(+)CD25^(+)调节性T细胞水平与CD8^(+)CD28^(+)调节性T细胞水平的相关性。采用多因素logistic回归分析早期CC患者腹腔镜根治术后预后不良的影响因素。采用受试者工作特征(ROC)曲线评估外周血CD4^(+)CD25^(+)、CD8^(+)CD28^(+)调节性T细胞水平对早期CC患者腹腔镜根治术后预后不良的预测价值。结果预后不良组CD4^(+)CD25^(+)调节性T细胞、癌胚抗原(CEA)、糖类抗原125(CA125)水平,术后切缘阳性占比以及术中宫旁浸润占比高于预后良好组,CD8^(+)CD28^(+)调节性T细胞水平低于预后良好组,差异有统计学意义(P<0.05)。Spearman秩相关分析结果显示,早期CC患者外周血CD4^(+)CD25^(+)调节性T细胞水平与CD8^(+)CD28^(+)调节性T细胞水平呈负相关(r_(s)=-0.478,P<0.05)。多因素logistic回归分析结果显示,较高的CEA、CA125、CD4^(+)CD25^(+)调节性T细胞水平是促进早期CC患者腹腔镜根治术后预后不良发生的独立危险因素(P<0.05),较高的CD8^(+)CD28^(+)调节性T细胞水平是抑制早期CC患者腹腔镜根治术后预后不良发生的独立保护因素(P<0.05)。ROC曲线分析结果显示,外周血CD4^(+)CD25^(+)、CD8^(+)CD28^(+)调节性T细胞水平能有效预测早期CC患者腹腔镜根治术后预后不良(P<0.05),两项指标联合可进一步提高预测效能[AUC(95%CI)=0.939(0.898~0.979),P<0.001],灵敏度和特异度分别为86.00%、88.20%。结论外周血CD4^(+)CD25^(+)、CD8^(+)CD28^(+)调节性T细胞水平与早期CC患者腹腔镜根治术后预后不良有关,二者能有效预测早期CC患者腹腔镜根治术后预后不良。

口腔扁平苔藓组织中IL-35表达水平与外周血CD4^(+)CD25^(+)Treg的相关性

目的探究口腔扁平苔藓(OLP)组织中白介素-35(IL-35)表达水平与外周血CD4^(+)CD25^(+)调节性T细胞(Treg)的相关性。方法收集2016年9月至2018年9月期间我院口腔科收治的口腔扁平苔藓患者38例,设为OLP组,另选同期来我院体检的健康人群20例作为对照组。取两组外周静脉血,荧光定量PCR检测外周血单个核细胞内IL-35两个亚基EB病毒诱导蛋白3(EBI3)、IL-12 p35和CD4^(+)CD25^(+)Treg标志物叉状头转录因子P3(FOXP3)的表达,酶联免疫吸附法检测血清IL-35、白介素-10(IL-10)、白介素-17(IL-17)和转化生长因子β1(TGF-β1)的含量,流式细胞仪检测外周血CD4^(+)CD25^(+)Treg细胞水平,分析IL-35和CD4^(+)CD25^(+)Treg水平与OLP患者临床病理特征之间的关系,Pearson法分析EBI3、IL-35与CD4^(+)CD25^(+)Treg的相关性。结果与对照组相比,OLP组外周血单个核细胞内EBI3、IL-12 p35、FOXP3的mRNA均显著上调(P<0.05)。与对照组相比,OLP组患者血清IL-35、IL-10、IL17、TGF-β1含量显著均上调,差异有统计学意义(P<0.05);与对照组相比,OLP组患者外周血CD4^(+)T细胞和CD4^(+)CD25^(+)Treg细胞水平均显著下降,差异有统计学意义(P<0.05);IL-35和CD4^(+)CD25^(+)Treg水平与患者基底细胞变性程度有关(P<0.05),而与性别、年龄、病程、疾病类型和淋巴细胞浸润无关(P>0.05)。OLP中IL-35与外周血CD4^(+)CD25^(+)Treg的水平呈正相关(r=0.3644,P<0.05)。结论OLP中IL-35与CD4^(+)CD25^(+)Treg表达均显著升高,且二者具有正相关关系。

急性非ST抬高心肌梗死患者外周血CD4^(+)CD25^(+)Foxp3^(+)Treg和IL-27变化的临床意义

目的观察急性非ST抬高心肌梗死患者(Non-STMI)治疗过程中外周血CD4^(+)CD25^(+)T/CD4^(+)T、CD4^(+)CD25highFoxp3^(+)Treg/CD4^(+)CD25high T比例及细胞因子TGF-β1、IL-10、INF-γ、IL-27的浓度变化,阐明Non-STMI患者是否存在调节性T细胞比例及功能异常。方法选取2012年8月至2016年4月入住中山大学附属第一医院急诊病房和CCU的Non-STMI40名,不稳定性心绞痛(UA)患者19名和同龄健康志愿者20名作为对照组(HC)。患者诊断明确后次日清晨和经规范治疗后5~7天,留取空腹外周血标本。流式细胞术检测CD4^(+)CD25^(+)T/CD4^(+)T、CD4^(+)CD25high Foxp3^(+)Treg/CD4^(+)CD25high T细胞。ELISA法检测细胞因子TGF-β1、IL-10、INF-γ和IL-27浓度。结果UA、Non-STMI组CD4^(+)CD25highFoxp3^(+)Treg/CD4^(+)CD25highT比例较HC组减低(P<0.01);Non-STMI明显低于UA组(P<0.05),而治疗后Non-STMI组Foxp3^(+)Treg细胞比例显著升高(P<0.05)。入院时Non-STMI和UA组TGF-β1、IL-10浓度较HC组明显降低(P<0.05),而INF-γ和IL-27浓度明显升高(P<0.05)。治疗后Non-STMI组IL-27迅速下降,而UA组变化不明显。治疗前IL-27浓度与CD4^(+)CD25highFoxp3^(+)Treg/CD4^(+)CD25highT呈线性负相关、与INF-γ浓度呈线性正相关。治疗后只有IL-27和CD4^(+)CD25high Foxp3^(+)Treg/CD4^(+)CD25highT呈线性负相关。结论Non-STMI患者CD4^(+)CD25highFoxp3^(+)Treg比例及功能异常,细胞因子IL-27有助于判断患者的临床疗效。

类风湿性关节炎患者抗环瓜氨酸肽抗体、CD4^(+)CD25^(+)调节性T细胞与类风湿因子的检验价值分析

目的探讨抗环瓜氨酸肽抗体(Anti-CCP)、CD4^(+)CD25^(+)调节性T细胞与类风湿因子(RF)在类风湿性关节炎患者中的检出结果及意义。方法选取2019年1月至2021年5月的昆明市延安医院的130例疑似类风湿性关节炎患者作为研究对象,进行Anti-CCP、CD4^(+)CD25^(+)调节性T细胞与RF水平进行检测,以晨僵持续1 h以上、3组关节肿胀与特异性抗体检测阳性作为金标准,分析上述指标的诊断效能,并对比类风湿性关节炎患者和非类风湿性关节炎患者的上述指标水平、不同活动度的类风湿性关节炎患者的指标水平。结果RF、Anti-CCP与CD4^(+)CD25^(+)调节性T细胞检测对类风湿性关节炎的诊断准确率为97.69%、特异度为98.11%、敏感度为97.70%、阳性预测值为98.84%、阴性预测值为96.30%。类风湿性关节炎患者的RF平均值、Anti-CCP平均值指标高于非类风湿性关节炎患者,且CD4^(+)CD25^(+)调节性T细胞低于非类风湿性关节炎患者,差异有统计学意义(P<0.05)。高度活动组患者RF平均值、Anti-CCP平均值高于低中度活动组,且CD4^(+)CD25^(+)调节性T细胞低于低中度活动组,差异有统计学意义(P<0.05)。结论Anti-CCP、CD4^(+)CD25^(+)调节性T细胞与RF指标检测有助于对类风湿性关节炎患者进行鉴别,并判断患者的疾病活动情况。

25-羟基胆固醇通过调控干扰素调节因子4抑制CD4^(+)T细胞IL-17的表达

目的探讨25-羟基胆固醇(25-hydroxycholesterol,25-HC)对人外周血单个核细胞(PBMCs)产生IL-17的抑制作用及其机制。方法用抗人CD3抗体联合抗CD28抗体刺激培养外周血单个核细胞(PBMCs)和纯化CD4^(+)T细胞,加或不加25-HC进行培养,ELISA、ELISPOT和PCR检测IL-17和IFN-γ的表达;流式细胞术检测T细胞转录因子(RORγt、RUNX1和IRF4)的表达、细胞表面活化分子的表达以及T细胞分裂增殖情况。通过电转染过表达IRF4,检测其对IL-17表达的影响。结果25-HC呈时间和剂量依赖的方式抑制T细胞产生IL-17,且主要抑制CD4^(+)T细胞中IL-17的表达。进一步研究发现,25-HC抑制T细胞晚期活化分子的表达和细胞分裂增殖,抑制IL-17相关转录因子IRF4的表达;过表达IRF4后,25-HC对IL-17表达的抑制作用被恢复。结论25-HC抑制Th17细胞的活化和增殖,并通过下调IRF4的表达抑制CD4^(+)T细胞产生IL-17。25-HC可能是IL-17相关炎症性疾病的一个具有前景的调控靶点。

冷冻消融联合养肺方治疗对Lewis肺癌CD4^(+)CD25^(+)Foxp3^(+)Treg的影响及机制

目的 探讨冷冻消融联合养肺方治疗对Lewis肺癌CD4^(+)CD25^(+)Foxp3^(+)Treg细胞影响及机制。方法 构建C57BL/6小鼠肺癌皮下移植瘤模型,随机分为模型组、冷冻消融组、冷冻消融联合养肺方低、中、高剂量组,每组5只。模型组行假手术,在小鼠移植瘤部位切开缝合,其余各组均行双循环冷冻消融术。术后冷冻消融联合养肺方低、中、高剂量组分别给予1.64、3.28、6.56 g/kg养肺方灌胃,模型组、冷冻消融组分别给予等体积的生理盐水灌胃,均1次/d,连续给药14 d。给药期间观察并记录各组小鼠肿瘤体积,末次给药后剥离脾脏和移植瘤,称取瘤体质量并计算抑瘤率;流式细胞术检测脾脏CD4^(+)T、CD4^(+)CD25^(+)Foxp3^(+)T细胞比例;qRT-PCR和Western blot法检测瘤组织中Foxp3表达。结果 各组小鼠肿瘤体积均逐渐增长,增长速度为模型组>冷冻消融组>冷冻消融联合养肺方低剂量组>冷冻消融联合养肺方中剂量组>冷冻消融联合养肺方高剂量组。手术联合药物干预14 d后,与模型组比较,各治疗组肿瘤体积小(P<0.05);与冷冻消融组比较,冷冻消融联合养肺方低、中、高剂量组肿瘤体积小(P<0.01);冷冻消融联合养肺方高剂量组肿瘤体积小于低剂量组(P<0.01)。与模型组比较,冷冻消融联合养肺方低、中、高剂量组瘤体质量小(P<0.05);与冷冻消融组比较,冷冻消融联合养肺方低、中、高剂量组瘤体质量小(P<0.05)。冷冻消融组、冷冻消融联合养肺方低、中、高剂量组抑瘤率逐渐升高。与模型组比较,各治疗组CD4^(+)T细胞比例低(P<0.05),冷冻消融联合养肺方低、中、高剂量组的CD4^(+)CD25^(+)Foxp3^(+)T%细胞比例低(P<0.05);与冷冻消融组比较,冷冻消融联合养肺方高剂量组CD4^(+)T、CD4^(+)CD25^(+)Foxp3^(+)T细胞比例低(P<0.05);冷冻消融联合养肺方低、中、高剂量组CD4^(+)T、CD4^(+)CD25^(+)Foxp3^(+)T细胞比例逐渐降低。与模型组比较,冷冻消融联合养肺方低、中、高剂量组肿瘤组织中Foxp3 mRNA相对表达量和Foxp3蛋白表达均降低(P<0.01);冷冻消融联合养肺方中、高剂量组Foxp3 mRNA相对表达量和Foxp3蛋白表达均低于冷冻消融组(P<0.01)和冷冻消融联合养肺方低剂量组(P<0.05)。结论冷冻消融联合养肺方通过减少Lewis肺癌小鼠脾脏CD4^(+)CD25^(+)Foxp3^(+)Treg细胞比例,下调肿瘤组织中Foxp3表达,发挥抑制Lewis肺癌增殖作用,其机制可能与增强机体抗肿瘤免疫应答,改善肿瘤免疫抑制有关。

Effects of estrogen on CD4^+ CD25^+ regulatory T cell in peripheral blood during pregnancy

Objective To investigate the effects of estrogen(E_2)level on regulatory T cells(Treg)in peripheral blood during pregnancy.Methods:A total of 30 healthy non-pregnant women were selected as control group,90 pregnant women of early,middle and late pregnancy and 30 postpartum women at 1 month after parturition were selected as experimental groups including early pregnancy group,middle pregnancy group and late pregnancy group;the proportions of CD4^+CD25^+Treg and CD4^+CD25^+CD127^-Treg among CD4 T cells were detected by flow cytometry;the serum estrogen content in peripheral blood was detected by electrochemical immune luminescence method.Results:E_2 level was coincident with the change of Tregs number during pregnancy.The estrogen content in peripheral blood increased gradually from early pregnancy to late pregnancy,then decreased significantly after parturition,and the level at 1 month after parturition down to the level in non-pregnancy group(P>0.05);the level of E_2 in pregnancy groups were significantly higher than those in non-pregnancy group(P<0.01);and there were significant differences among early pregnancy group,middle pregnancy group and late pregnancy group(P<0.05).The proportions of CD4^+CD25^+Treg and CD4^+CD25^+CD127^-Treg in pregnancy groups were significantly higher than those in non-pregnancy group(P<0.05),but decreased significantly after parturition,and there was no significant difference between non-pregnancy group and postpartum women group(P>0.05):the proportions in middle and late pregnancy groups were significantly higher than those in early pregnancy group(P<0.05).but decreased slightly in late pregnancy group,there was no significant difference between late pregnancy group and middle pregnancy group(P>0.05).There was correlation between Tregs number with estrogen level during pregnancy.The proportion of CD4^+CD25^+Treg and CD4^+CD25^+CD 127^-Treg were positively correlated with estrogen level.Conclusions:High proportion of CD4^+CD25^+Trcg and CD4^+CD25^+CD127^-Treg is closely related to the high level of E,during pregnancy.It suggested that high level of estrogen may induce an increase of CD4^+CD25^+Treg in peripheral blood.and then influence the immune function of pregnant women.The results of this experiment might play an important role of estrogen in immune-modulation during pregnancy.

Analysis of CD4^+CD25^+ Regulatory T Cells and Foxp3 mRNA in the Peripheral Blood of Patients with Asthma

The changes of CD4^＋CD25^＋ regulatory T cells （CD4^＋CD25^＋ Treg） and Foxp3 mRNA in peripheral blood mononuclear cells （PBMCs） from patients with asthma were investigated in order to elucidate the possible roles of CD4^＋CD25^＋ Treg in the development of asthma. The peripheral blood samples were collected from 29 healthy controls （normal control group） and 78 patients with asthma which included 30 patients in exacerbation group, 25 patients in persistent group, and 23 patients in remission group. By using flow cytometry and RT-PCR, the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs were detected. The CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs of exacerbation and persistent groups were lower than that of remission and normal control groups （P〈0.05）. Although the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA of remission group were also lower than that of normal control group, there was no significant difference between them （P〉0.05）. As compared with persistent group, exacerbation group had lower CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA （P〈0.05）. It was indicated that the decrease of CD4^＋CD25^＋ Treg ratio and its function in PBMCs may be responsible for pathogenesis of asthma.

An Association between Immunosenescence and CD4^+CD25^+ Regulatory T Cells: A Systematic Review

Objective Age-related increment of the prevalence of CD4^＋CD25^＋ regulatory T （Treg） cells were described controversially, and whether such changes explain immune dysfunction in the elderly is still unclear. The aim of this systematic review is to evaluate the role of the Tregs in immunosenescence. Methods Medline and manual searches were performed to identify all published epidemiological and animal studies investigating the efficacy of the association between immunosenescence and Treg cells. Results It was founded that the frequency, phenotypic characteristics, and number/function of Tregs were altered significantly with aging. Medical conditions in individuals with advanced ageas well as apoptosis intensity of Treg cells had an impact on the accumulation of Tregs which in turn could deteriorate cytotoxic activity of CD8＋ T and NK cells and production of IL-2. The range of immune cells that could be suppressed by Treg cells was quite wide and covered CD4^＋CD25^＋ T cells, NK cells, dendritic cells and even monocytes. These changes were observed both in humans and experimental animals. Besides, it was believed that frequency of Tregs increased with age and was accompanied by intensified suppressive activity for Tregs in patients, for example, with Alzheimer disease （AD） and Parkinson disease （PD）. The impaired condition of CD4＋ T cells, so-called immunosenescence, rendered transplant recipients less responsive to an allogeneic kidney graft, an effect that was limited to transplant recipients who were aged over 60 years. Conclusions Treg cells are associated with immunosenescence. All these changes contribute to the aging-related decline of immune responses and lead to the higher risk of immune-mediated diseases, cancer or infections in aged individuals.

Changes of CD4^+CD25^+ Regulatory T Cells in Patients with Acute Coronary Syndrome and the Effects of Atorvastatin

The function of CD4＋CD25＋ regulatory T lymphocytes （Treg） in patients with acute coronary syndrome （ACS） and the effects of atorvastatin were investigated. Forty-eight patients with ACS were randomly divided into two groups： group C receiving conventional therapy （n=24）, and group C＋A receiving conventional therapy＋atorvastatin （10 mg/day, n=24）. T lymphocytes from ACS patients （before and 2 weeks after the treatment） or 18 healthy subjects were separated and the flow cytometry was used to measure the percentage of Treg. The inhibitory ability of Treg on effector T cells was determined by mixed lymphocyte reaction （MLR）. ELISA was used to measure the serum levels of cytokines （IL-10, TGF-β1 and IFN-γ） before and after treatment. The results showed that as compared with normal control group, Treg percentage was decreased significantly （P〈0.01）, the inhibitory ability of Treg on the T lymphocytes proliferation was reduced （P〈0.01）, IFN-γ levels were increased and IL-10 and TGF-β1 levels were lowered in ACS patients. After treatment with atorvastatin, Treg percentage and the inhibitory ability of Treg on T lymphocytes proliferation were significantly increased in ACS patients. Serum IFN-γ was decreased significantly, while IL-10 and TGF-β1 were elevated significantly as compared with the non-atorvastatin group. The number of Treg was positively correlated with serum TGF-β1, but negatively with serum IFN-γ and CRP. It was concluded that ACS was associated with decreased number and defected function of Treg, which may play an important role in initiating immune-inflammatory response in ACS. The inhibitory effects of atorvastatin on inflammation in ACS may be due to its beneficial effects on Treg and restoration of immune homeostasis.

Depletion of CD4^+CD25^+ regulatory T cells can promote local immunity to suppress tumor growth in benzo[a]pyrene-induced forestomach carcinoma

AIM： To elucidate the distribution of CD4^＋CD25^＋ regulatory T cells （Tregs） in different lymphoid tissues and its local enhancement on tumor growth before and after depletion of CD4^＋CD25^＋ Tregs. METHODS： Female ICR mice were garaged with benzo[a]pyrene （BaP） to induce forestomach carcinoma. CD4^＋CD25^＋ Tregs were intraperitoneally depleted with monoclonal antibody PC61. These mice were divided into BaP-only, BaP ＋ IgG, BaP ＋ PC61, and control groups. The forestomach of mice was dissected for histological analysis, and tunnel test was performed for apoptosis of tumor cells. CD4^＋CD25^＋ Tregs were sorted from different lymphoid tissues and expression of Foxp3, IL-10, and chemokine receptors was analyzed by flow cytometry, semi-quantitative and veal-time polymerase chain reaction. RESULTS： The mice gavaged with only BaP showed increased forestomach papilloma and carcinoma at wk 16 and 32. The proportion of CD4^＋CD25^＋ Tregs was significantly higher in peri-stomach regional lymph nodes than in other lymphoid tissues. These CD4^＋CD25^＋ Tregs in regional lymph nodes expressed higher levels of Foxp3 and IL-10, enriched in the CD62L-subset, and CCR1 and CCR5 chemokine receptors. In mice gavaged with BaP ＋ PC61, the number of tumor nodules and tumor volume decreased significantly with massive infiltrating cells and apoptosis of tumor cells. In the draining regional lymph nodes, the number of CD4^＋CD25^＋ Tregs also decreased significantly. CONCLUSION： Inducible and activated CD4^＋CD25^＋ Tregs in the draining regional lymph nodes suppress host local immunity during tumor growth. Depletion of CD4^＋CD25^＋ Tregs can promote host local immunity to suppress tumor growth.

Downregulation of CD4+CD25+ regulatory T cells may underlie enhanced Th1 immunity caused by immunization with activated autologous T cells

Regulatory T cells （Treg） play important roles in immune system homeostasis, and may also be involved in tumor immunotolerance by suppressing Th1 immune response which is involved in anti-tumor immunity. We have previously reported that immunization with attenuated activated autologous T cells leads to enhanced anti-tumor immunity and upregulated Thl responses in vivo. However, the underlying molecular mechanisms are not well understood. Here we show that Treg function was significantly downregulated in mice that received immunization of attenuated activated autologous T cells. We found that Foxp3 expression decreased in CD4＋CD25＋ T cells from the immunized mice. Moreover, CD4＋CD25＋Foxp3＋ Treg obtained from immunized mice exhibited diminished immunosuppression ability compared to those from naive mice. Further analysis showed that the serum of immunized mice contains a high level ofanti-CD25 antibody （about 30 ng/ml, p〈0.01 vs controls）. Consistent with a role ofanti-CD25 response in the downregulation of Treg, adoptive transfer of serum from immunized mice to naive mice led to a significant decrease in Treg population and function in recipient mice. The triggering of anti-CD25 response in immunized mice can be explained by the fact that CD25 was induced to a high level in the ConA activated autologous T cells used for immunization. Our results demonstrate for the first time that immunization with attenuated activated autologous T cells evokes anti-CD25 antibody production, which leads to impeded CD4＋CD25＋Foxp3＋ Treg expansion and function in vivo. We suggest that dampened Treg function likely contributes to enhanced Thl response in immunized mice and is at least part of the mechanism underlying the boosted anti-tumor immunity.

Influence of Danshen Injection on airway inflammation and CD4^+ CD25^+ regulatory T cells of asthmatic rats

Objective： To investigate the influence of Danshen Injection on airway inflammation and CD4^＋CD25^＋ regulatory T cells（CD4^＋CD25^＋ Tr） of asthmatic rats, and elucidate the possible mechanism of Danshen Injection in treatment of asthma. Methods： 30 Wister rats were randomly divided into control group, asthma group and Danshen Injection treated group. Bronchoalveolar lavage fluids （BALF） were collected, and cytology studies were conducted. Lung tissues were obtained and pathologic analyses were done with hematoxylin and eosin stain （HE）. Flow cytometry was used to detect the CD4^＋CD25^＋ Tr ratio in peripheral blood mononuclear cells （PBMCs）. Results： Total cell, the percentage of lymphocytes, neutrophils and eosinophils （Eos） in BALF of Danshen Injection-treated group were lower than that in asthma group （P〈0.05, P〈0.01）. Compared with asthma group, less infiltration of inflammatory cells in lung tissues was observed in Danshen Injection-treated group. CD4^＋CD25^＋ Tr of asthma group was lower than that of control and Danshen Injection treated group （P〈0.05）. Conclusion： Danshen Injection can suppress airway inflammation of asthmatic rats, probably by increasing the number of CD4^＋CD25^＋ Tr.

CD4^＋CD25^＋CD127^low识别人外周血CD4^＋CD25^＋调节性T细胞的优势

目的:探索可用于检测人外周血中CD4+CD25+Treg细胞的最佳标记物。方法:以52名健康人和47名非血液系统肿瘤患者为研究对象,采用多色免疫荧光素标记和多参数流式细胞术同时检测外周血中CD4+CD25high、CD4+CD25+FoxP3+和CD4+CD25+CD127lowT细胞,并以经典指标CD4+CD25high和CD4+CD25+FoxP3+为标准,分析和比较CD4+CD25+CD127low作为识别CD4+CD25+Treg细胞的可行性及其优势。结果:健康人和肿瘤患者外周血CD4+CD25high、CD4+CD25+FoxP3+和CD4+CD25+CD127lowT细胞占CD4+T细胞百分比分别为(1.769±0.682)和(2.958±1.392);(2.905±1.772)和(5.128±2.227)以及(5.396±1.306)和(7.175±2.565)。三者呈相同的趋势,即肿瘤患者组>健康人组,且三者之间呈显著正相关(P<0.05)。用两种不同标记法高纯度分选CD4+CD25high和CD4+CD25+CD127lowT细胞群后,FoxP3阳性率分别为90.9和92.7。结论:CD4+CD25+CD127low三标记法可以帮助识别CD4+CD25+Treg和部分激活的CD4+T细胞,提高CD4+CD25+Treg细胞的可检出数量,并且不影响细胞活性度,是反映CD4+CD25+Treg细胞更理想的指标。

中国健康人外周血中具有CD4^+CD25^(nt/hi)CD127^(lo)特征的调节性T细胞频率

目的:初步确定健康人外周血中具有CD4+CD25nt/hiCD127lo特征的调节性T细胞(Treg)频率,为临床相关疾病的研究及Treg的分选提供参考。方法:采集312名8~60岁(5个年龄组)、不同性别健康人的静脉血,经三重免疫荧光染色,用流式细胞术分析CD4+CD25nt/hiCD127loTreg细胞频率,并观察细胞内Foxp3转录因子的表达。结果:健康人CD4+CD25nt/hiCD127loTreg细胞在外周血中约占CD4+T细胞的(6.55±0.11)%,各年龄组之间有差异(P=0.015),组内性别之间也存在统计学意义(P<0.05);CD25nt/hiCD127lo细胞特异性地表达Foxp3转录因子。结论:初步确定了中国健康人外周血中具有CD4+CD25nt/hiCD127lo表达特征的细胞频率,为Treg细胞的临床研究奠定了基础;CD25nt/hiCD127lo作为CD4+CD25+Treg细胞表面的特征性标志,可在分选时排除其他细胞干扰,获得较完整的Treg细胞。

TF3-H4对CD4^+CD25^+调节性T细胞和CD4^+CD25^-效应性T细胞早期活化的影响

目的观察1',2',3',7'-四氢茶黄素-3,3'-双没食子酸酯(TF3-H4)对CD4+CD25+调节性T细胞和CD4+CD25-效应性T细胞早期活化的影响,分析TF3-H4对两群功能相反的CD4+T细胞的作用。方法免疫磁珠分离BALB/c小鼠脾脏CD4+CD25+调节性T细胞和CD4+CD25-效应性T细胞,加入ConA或PDB,和TF3-H4共同孵育12 h后收集细胞,流式细胞仪分析细胞表面早期活化标志CD69的表达。结果在ConA和PDB的作用下,两群细胞早期活化标志CD69的表达均升高。TF3-H4(20 mg.L-1)不能抑制PKC激动剂PDB激活的CD4+CD25-效应性T细胞CD69的表达,却能抑制ConA激活的CD4+CD25-效应性T细胞CD69表达;同时,TF3-H4也能明显抑制ConA激活的CD4+CD25+调节性T细胞的CD69表达。结论茶黄素衍生物TF3-H4可能经由TCR活化途径的上游抑制CD4+CD25-效应性T细胞活化;TF3-H4对CD4+CD25+调节性T细胞和CD4+CD25-效应性T细胞早期活化的抑制作用,可能是该类化合物发挥抗炎、抗肿瘤作用的机制之一。

糖皮质激素对哮喘小鼠CD4^+CD25^+调节性T细胞的作用

目的建立哮喘小鼠动物模型并给予吸入糖皮质激素治疗,通过分析CD4+CD25+调节性T细胞及相关细胞因子在哮喘发病中的变化情况,以及观察糖皮质激素治疗对该细胞的影响和干预作用,探讨哮喘发作的新的免疫机制。方法分别制备哮喘组(OVA),哮喘吸入激素治疗组(OVA/GC)及对照组(NS)动物模型。所有小鼠于末次激发后24 h,取右肺中叶标本作病理切片,观察炎症改变;同时收集外周抗凝血及肺组织细胞悬液,通过流式细胞仪及酶联免疫吸附试验(ELISA)测定CD4+CD25+调节性T细胞占CD4+T细胞的百分比和血浆中IL-10和TGF-β1水平。结果①哮喘小鼠外周血及肺组织细胞悬液中CD4+CD25+调节性T细胞百分率明显降低(P<0.01),吸入糖皮质激素治疗后CD4+CD25+调节性T细胞百分率明显升高(P<0.01)。②IL-10在哮喘组较对照组明显降低(P<0.01),糖皮质激素治疗组IL-10明显升高(P<0.01)。③TGF-β1在哮喘小鼠较对照组明显降低(P<0.01),吸入糖皮质激素治疗后TGF-β1有所升高,但差异无显著性。结论①哮喘小鼠CD4+CD25+调节性T细胞和IL-10,TGF-β1数量和分泌水平降低,尤其是肺部CD4+CD25+调节性T细胞不能有效聚集,导致全身和局部对炎症反应的抑制作用减弱,可能是导致哮喘发生的原因之一。②糖皮质激素可通过增加CD4+CD25+调节性T细胞及IL-10数量和分泌水平,增强其免疫抑制功能而发挥治疗作用。③外周血和肺部CD4+CD25+调节性T细胞百分率变化具有一致性,监测外周血CD4+CD25+调节性T细胞变化情况可以了解肺部变化趋势。

慢性乙型肝炎患者外周血CD4^＋CD25^＋Treg与CD4^＋和CD8^＋ T淋巴细胞亚群的相关研究

目的:探讨慢性乙型肝炎患者外周血中CD4+CD25+调节性T细胞的含量和CD4+CD8+T淋巴细胞亚群分布,两者之间相关性及与HBV的相关性。方法:采用流式细胞术检测50例慢性乙型肝炎患者和20例健康对照者外周血中CD4+CD25high、CD4+CD25+Foxp3+Treg细胞表达及CD3/CD4/CD8 T淋巴细胞亚群,荧光定量PCR法检测HBV DNA含量。结果:慢性乙型肝炎患者外周血中CD4+CD25highTreg明显高于健康对照组(P<0.01),且随HBV DNA载量增加,患者外周血中CD4+CD25highTreg细胞的水平逐渐升高。慢性乙型肝炎患者外周血中CD4+CD25+Foxp3+Treg细胞也相应增高,且与CD4+CD25highTreg细胞的变化成正相关(r=0.890,P<0.001)。与健康对照组比较,患者组CD4+T细胞百分率及CD4+/CD8+比值均降低,而CD3+T细胞和CD8+T细胞百分率差异无显著性(P>0.05)。CD4+CD25highTreg细胞与HBV DNA取对数后成正相关(r=0.782,P<0.001),与谷丙转氨酶(ALT)成正相关(r=0.432,P<0.005);与CD3+、CD4+、CD8+T细胞水平及CD4+/CD8+比值均无相关性(P>0.05)。CD3+、CD4+、CD8+T淋巴细胞及CD4+/CD8+比值与HBV DNA载量之间亦无相关性(P>0.05)。结论:慢性乙型肝炎患者外周血中CD4+CD25+Treg细胞增高,且与HBV的复制水平及ALT增高具有一致性,而T细胞亚群是否可作为监测CHB患者免疫状态的指标需进一步探讨。

肺癌患者CD4^+ CD25^(high) Foxp3^+调节性T细胞的格局变化及意义

目的:研究肺癌患者外周血(PBMC)及肿瘤浸润淋巴细胞(TIL)中CD4+CD25h ighFoxp3+调节性T细胞(Treg)的比例改变,探讨其在抗肿瘤免疫中的调节作用。方法:分离肺癌患者PBMC及TIL,FACS分析CD4+/CD8+T细胞的比值及CD4+CD25h ighT细胞占CD4+T细胞的比例。Real-tim e PCR检测Treg特异性转录因子Foxp3基因在PBMC及TIL中的表达。结果:肺癌患者PBMC及TIL中CD4+/CD8+比值降低;而CD4+CD25h ighT细胞在CD4+T细胞中所占比例升高;Foxp3基因仅在TIL中高表达,而在PBMC中低或不表达,表明肿瘤局部的CD4+CD25h ighT细胞主要是CD4+CD25h ighFoxp3+Treg。结合临床资料分析显示Treg在肺腺癌比例较高。结论:CD4+CD25h ighFoxp3+Treg在肺癌患者肿瘤浸润淋巴细胞中明显升高,可能与其通过细胞与细胞间接触抑制CD8+T细胞的杀伤效应,最终发挥免疫抑制效应相关。