Analysis of CD4^+CD25^+ Regulatory T Cells and Foxp3 mRNA in the Peripheral Blood of Patients with Asthma

The changes of CD4^＋CD25^＋ regulatory T cells （CD4^＋CD25^＋ Treg） and Foxp3 mRNA in peripheral blood mononuclear cells （PBMCs） from patients with asthma were investigated in order to elucidate the possible roles of CD4^＋CD25^＋ Treg in the development of asthma. The peripheral blood samples were collected from 29 healthy controls （normal control group） and 78 patients with asthma which included 30 patients in exacerbation group, 25 patients in persistent group, and 23 patients in remission group. By using flow cytometry and RT-PCR, the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs were detected. The CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs of exacerbation and persistent groups were lower than that of remission and normal control groups （P〈0.05）. Although the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA of remission group were also lower than that of normal control group, there was no significant difference between them （P〉0.05）. As compared with persistent group, exacerbation group had lower CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA （P〈0.05）. It was indicated that the decrease of CD4^＋CD25^＋ Treg ratio and its function in PBMCs may be responsible for pathogenesis of asthma.

Rapamycin increased development of CD4^+ CD25~h ighFoxp3^+ T cells of peripheral blood in liver transplant recipients

Objective To investigate the possible influence of immunosuppressive therapy,including sirolimus ( SRL) and calcineurin inhibitors ( CNI,tacrolimus) ,on level of Treg in liver allo - graft recipients. Methods Forty - seven liver transplant recipients with stable liver function were assessed for at least 2 years,and divided into

中国HIV/AIDS患者CD_4^+CD_8^+T淋巴细胞与外周血各组份间关系的研究

目的 研究艾滋病病毒 /艾滋病 (HIV/AIDS)患者CD+ 4 、CD+ 8T淋巴细胞数与外周血各组份间白细胞(WBC)、血小板 (PLT)、血红蛋白 (HGB)、粒细胞百分数 (GR % )、淋巴细胞百分数 (LY % )、LY的相关性 ,为临床治疗提供参考依据。方法 HIV/AIDS患者抗凝外周血 5 13份 ,在 6小时之内检测其血细胞分类和CD+ 4 、CD+ 8T淋巴细胞计数 ,比较CD+ 4 、CD+ 8T淋巴细胞计数与WBC、HGB、PLT、LY %、GR %和LY的相关性。结果 CD4/CD8全部倒置 ,其中CD4/CD8<1者达 94 7% ;CD+ 4 细胞数 <5 0 0 /mm3 与HGB (r=0 16 0 ,P <0 0 1)、PLT (r=- 0 0 14 ,P <0 0 1)、GR % (r=- 0 2 81,P<0 0 1)、LY % (r =0 32 1,P <0 0 1)、LY((r =0 4 94 ,P <0 0 1)相关均有非常显著的统计学意义 ;CD+ 8细胞数与WBC数 (r=0 112 ,P <0 0 5 )、HGB (r=0 131,P <0 0 1) 、GR % (r=- 0 5 2 6 ,P <0 0 1)、LY % (r=0 5 6 9,P <0 0 1)、LY (r=0 90 4 ,P <0 0 1) 相关均有显著性 ;2 0 0 /mm3 <CD+ 4 细胞数 <5 0 0 /mm3 与LY (r=0 2 79,P <0 0 1)、PLT (r=0 192 ,P <0 0 1) 相关有显著性 ,CD+ 4 细胞数 <2 0 0 /mm3 与LY (r =0 2 6 2 ,P<0 0 1)、LY % (r =0 2 79,P<0 0 1)、GR % (r =- 0 2 94 ,P<0 0 1)

广西壮族自治区健康成年人T淋巴细胞亚群分类绝对计数及CD_4/CD_8比值调查

目的 初步建立广西壮族自治区健康人群外周静脉血CD3 、CD4、CD8T淋巴细胞绝对数值和CD4/CD8比值的参考范围。方法 用流式细胞仪 (FACSCalibur,FCS)、三色免疫荧光试剂和绝对计数管 (TnTEST/Tru COUNT) ,检测 110名广西健康成年人 (16～ 5 6岁 )静脉全血CD3 、CD4、CD8T淋巴细胞绝对数值和CD4/CD8比值 ,并观察T淋巴细胞亚群分类计数在性别、年龄和民族上的差别。结果 110名健康成年人检测结果平均值 :CD3 为5 2 4± 5 4 1,CD4为 82 3± 30 5 ,CD8为 6 0 1± 2 4 8,CD4/CD8为 1 4 9± 0 5 3。进一步分析发现 ,CD4和CD4/CD8在民族分布上 (汉族 79名、壮族 31名 )的差异有显著的统计学意义 (P <0 0 5 )。结论 这次初步建立CD3 、CD4、CD8T细胞绝对数值和CD4/CD8比值正常参考范围 ,对于指导广西的艾滋病诊断及治疗工作有重要意义。

抗生素联合强的松治疗急性脓肿型非哺乳期乳腺炎的疗效观察及对外周血CD^4+/CD^8+比值的影响

目的:探讨采用抗生素联合强的松治疗急性脓肿型非哺乳期乳腺炎的临床疗效,及其对外周血CD^(4+)/CD^(8+)比值的影响,以及安全性评价。方法:选择乳腺外科2012年6月至2014年12月收治的120例急性脓肿型非哺乳期乳腺炎的患者作为观察对象,随机分为两组,每组60例;对照组予行脓肿引流配合口服阿莫西林片治疗,0.5g/次,tid;观察组在对照组的基础上加用强的松20mg,bid(早晚餐后),14d为1疗程,均于1个疗程后评价两组治疗效果差异及不良反应发生情况。结果:观察组的有效率86.4%(51/59),明显高于对照组的有效率75.9%(44/58),差异具有统计学意义(P<0.05)。并且观察组在痊愈率、痊愈时间方面均优于对照组(均P<0.05)。治疗后两组的外周血淋巴细胞CD^(4+)百分率CD^(4+)/CD^(8+)比值均有不同程度下降(均P<0.05),而观察组下降更为明显(P<0.05);观察组CD^(8+)百分率明显升高(P<0.05),对照组升高不明显(P>0.05),差异无统计学意义;CD^(4+)/CD^(8+)比值比较,观察组显著低于对照组,(P<0.05),差异具有统计学意义。两组均未见到明显的不良反应发生。结论:采用抗生素联合强的松治疗治疗非哺乳期急性脓肿型乳腺炎较单存使用抗生素疗效满意,痊愈时间快,安全性高,值得临床推广应用。

Effects of pituitary adenylate cyclase activating polypeptide on CD4^+/CD8^+T cell levels after traumatic brain injury in a rat model

BACKGROUND:The effect of pituitary adenylate cyclase activating polypeptide(PACAP)during traumatic brain injury(TBI) and whether it can modulate secondary injury has not been reported previously.The present study evaluated the potential protective effects of ventricular infusion of PACAP in a rat model of TBI.METHODS:Male Sprague Dawley rats were randomly divided into 3 treatment groups(n=6,each):sham-operated,vehicle(normal saline)^+TBI,and PACAP^+TBI.Normal saline or PACAP(1 ug/5uL) was administered intracerebroventricularly 20 minutes before TBI.Right parietal cortical contusion was produced via a weight-dropping method.Brains were extracted 24 hours after trauma.Histological changes in brains were examined by HE staining.The numbers of CD4^+ and CD8^+ T cells in blood and the spleen were detected via flow cytometry.RESULTS:In injured brain regions,edema,hemorrhage,inflammatory cell infiltration,and swollen and degenerated neurons were observed under a light microscope,and the neurons were disorderly arrayed in the hippocampi.Compared to the sham group,average CD4^+ CD8" lymphocyte counts in blood and the spleen were significantly decreased in rats that received TBI^+vehicle,and CD4^+ CD8^+ were increased.In rats administered PACAP prior to TBI,damage was attenuated as evidenced by significantly increased CD4^+,and decreased CD8^+,T lymphocytes in blood and the spleen.CONCLUSION:Pretreatment with PACAP may protect against TBI by influencing periphery T cellular immune function.

外周血CD4+调节性T细胞表达水平与糖尿病周围神经病变的相关研究

目的探讨外周血CD4+调节性T细胞表达水平与糖尿病周围神经病变(Diabetic Peripheral Neuropathy,DPN)的相关性。方法选取2016年8月到2019年2月间在我院诊治的DPN患者124例作为DPN组,同期选择无合并DPN的2型糖尿病患者124例作为非DPN组,调查与记录所有患者的一般资料、生化资料,检测外周血CD4+调节性T细胞表达水平并进行相关性分析。结果 DPN组患者的糖尿病病程显著长于非DPN组(P<0.05)。DPN组患者的糖化血红蛋白、尿微量白蛋白、LDL-C值与非DPN组对比,差异有统计学意义(P<0.05)。DPN组患者的CD4+调节性T细胞表达水平显著低于非DPN组(P<0.05)。在DPN组患者中,Spearman相关分析显示CD4+调节性T细胞表达水平与糖化血红蛋白、尿微量白蛋白、LDL-C、糖尿病病程都有显著相关性(P<0.05)。结论 DPN患者的外周血CD4+调节性T细胞表达水平呈现下降趋势,与患者的糖化血红蛋白、尿微量白蛋白、LDL-C、糖尿病病程有相关性。

Autologous Peripheral Blood Stem Cells and <i>γ</i>/<i>δ</i>T Cells May Improve Immunity in Treating Secondary Bacteremic Infection in HIV Infected Patient

Opportunistic bacteremia in adult HIV-infected patients is a normal co-infectious condition caused by Gram-negative bacilli. Respiratory infections, including cough, shortness of breath, and chest pain and skin infection with eruptions, pustules and itchiness, are common complaints in the setting of late HIV infection. The variety of infections ranges from mild, self-limited viral, bacteremia and fungal infections to severe, life-threatening demanding urgent care and hospitalization. Varicella pneumonia, for instance, is the most severe complication of chickenpox in HIV infected adults, potentially refractory, fulminant respiratory failure can ensue. Patients with impaired immune status and chronic lung disease are at an increased risk. In the United States as well as in Vietnam, bacterial/viral pneumonia and skin infection are the two most common HIV-associated conditions. While globally the incidence of opportunistic infection has decreased since the introduction of highly active antiretroviral therapy during the last 3 decades, HIV-associated diseases remain a significant source of mortality, thus any manifestation must be taken seriously. This study will present the most common HIV-related pulmonary and skin infections and provide an overview of the epidemiology, characteristic clinical and chest radiograph findings, diagnosis, treatment, and prevention globally as well in Vietnam. Though the extensive efforts of the Vietnamese Government during last decade contributed to a valuable decrease, yet epidemic in Vietnam still remains high, ranking Vietnam 5th in the South-East region. The second part of the study focuses on a unique and severe HIV case report of a 35-year-old man, with a rare form of pneumonia caused by Acitenobacter spp. concomitant with a prolonged and disseminating skin infection. The case has been treated with a combination of conventional anti-retroviral medication and autologous peripheral blood stem cells, the results showed that within 5 months there was an impressive amelioration of HIV viral activity together with a total recovery from pneumonia and skin infection.

Peripheral and Tissue Lymphocytes as Predictors of Pathological Response in Locally Advanced Rectal Cancer Post Neoadjuvant Chemoradiotherapy

Background: Tailoring therapy is the target in the management of any cancer;if factors which can predict response to treatment are identified, we can individualize treatment. Locally advanced rectal cancer studies reported that tumor microenvironment and host immune response played roles in sensitivity to chemoradiotherapy (CRT) by proving that both peripheral circulating lymphocytes and tumor infiltrating lymphocytes (TILs) strongly correlated with the response rate to CRT and it impacted disease outcome. Aim of the work: We aimed to assess the predictive value of peripheral blood lymphocytes and tumor infiltrating lymphocytes by correlation with regression rate post chemo-radiotherapy in patients with rectal cancer, and to find correlation between peripheral and tissue lymphocytes. Method: Before neoadjuvant, CRT venous blood samples were obtained from 40 patients with rectal cancer, and prior to surgery. Blood cell counts in the samples were analyzed using an automated hematology analyzer and flowcytometry used to analyze lymphocyte subsets. Colonscopic biopsies were obtained before the CRT;the numbers and distributions of T cells (CD4 & CD8) were evaluated by immunostaining. Results: Pre CRT peripheral total lymphocytes, T lymphocytes, T helper, T cytotoxic lymphocytes significantly correlated with tumor regression rate (p = 0.04, 0.05, 0.06, 0.04 respectively). The density of tissue CD4(+) and CD8(+) T cells was highly correlated with tumor regression post CRT (p = 0.01 for both). The high expressions of tissue CD4 & CD 8 were significantly correlated with high number of pretreatment peripheral total lymphocytes, T lymphocytes, T helper, and T cytotoxic lymphocytes with significant p value for all. Conclusion: We concluded that peripheral lymphocytic count and its subsets have significant correlation to tissue CD4, CD8 and both can predict pathological response to CRT;enhancement of lymphocytes mediated immune response can help for outcome improvement.

A reversed CD4/CD8 ratio of tumor-infiltrating lymphocytes and a high percentage of CD4^(+)FOXP3^(+) regulatory T cells are significantly associated with clinical outcome in squamous cell carcinoma of the cervix

In this study,40 biopsy samples collected from cervical cancer patients at the First Affiliated Hospital of Xi’an Jiaotong University,China,were retrospectively assessed using immunohistochemistry for CD4^(+) and CD8^(+) tumor-infiltrating lymphocytes(TILs)and were analyzed for the expression of FOXP3,OX40,granzyme B(GrB)and perforin(Prf).The proliferating index of the TILs was determined by assessing Ki67 expression.We determined the prognostic value of low and high numbers of TILs on survival by performing Kaplan–Meier analysis using median values as the cut-off points.Except for the number of CD4^(+)FOXP3^(+) regulatory T cells(Tregs)and the CD4/CD8 ratio,none of the CD4^(+),CD8^(+),OX401,GrB^(+) or Prf^(+) TILs were associated with the overall 5-year survival rate.The 5-year survival rate was significantly lower in patients who had a high percentage of Tregs as compared with the those who had a lower percentage(35.3%versus 88.9%,P50.001),while the 5-year survival rate was significantly higher in patients with a high CD4/CD8 ratio as compared with patients who had a low CD4/CD8 ratio(82.4%versus 44.4%,P50.029).When we considered the deaths and surviving cases as separate groups,we found that both the number of CD4^(+) T cells and the CD4/CD8 ratio were significantly lower in patients who died as compared with those who survived(26.33±11.80 versus 47.79±38.18,P=0.023 and 0.60±0.25 versus 1.17±1.02,P=0.019,respectively).In conclusion,decreased proportions of tumor-infiltrating CD4^(+) T cells with high percentages of Tregs and reversed CD4/CD8 ratios were significantly associated with the clinical outcome of patients with cervical carcinoma.

大面积脑梗死合并肺部感染外周血T淋巴细胞亚群及CD_(4)^(+)/CD_(8)^(+)比值水平变化及意义

目的探究大面积脑梗死(LHI)合并肺部感染(PI)患者外周血T淋巴细胞亚群及CD_(4)^(+)/CD_(8)^(+)比值水平的动态变化及意义。方法收集医院2011年3月-2017年5月收治的100例LHI合并PI患者资料进行回顾性分析,检测患者入院时、3 d、7 d外周血T淋巴细胞亚群水平,依据格拉斯哥昏迷评分(GCS)、临床肺部感染评分(CPIS)、28 d时改良Rankin量表评分评估患者LHI、PI病情严重程度以及预后情况并进行分组,比较不同病情严重程度以及预后患者入院后外周血T淋巴细胞亚群及CD_(4)^(+)/CD_(8)^(+)比值水平的动态变化及其临床意义。结果三组患者入院时、住院3 d、住院7 d时,重度组患者CD3+、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)比值低于轻度组与中度组,CD_(8)^(+)T均高于轻度组与中度组,中度组患者CD3+、CD_(4)^(+)T、CD_(4)^(+)/CD_(8)^(+)比值低于轻度组,CD_(8)^(+)T均高于轻度组(P<0.05);轻度组PI患者入院时CD3+、CD_(4)^(+)水平及CD_(4)^(+)/CD_(8)^(+)比值均高于中重度组,CD_(8)^(+)水平低于中重度组(P<0.05);预后良好组患者入院时CD3+、CD_(4)^(+)T淋巴细胞亚群水平及CD_(4)^(+)/CD_(8)^(+)比值高于预后不佳组,CD_(8)^(+)水平低于预后不佳组(P<0.05);ROC曲线结果显示患者入院时CD3+、CD_(8)^(+)、CD_(4)^(+)以及CD_(4)^(+)/CD_(8)^(+)预测患者预后曲线下面积(AUC)分别为0.855、0.871、0.729、0.557。结论LHI合并PI患者外周血T淋巴细胞亚群及CD_(4)^(+)/CD_(8)^(+)比值水平动态变化与患者病情严重程度有关,可用于预测患者预后情况。

鸡外周血T淋巴细胞分群及免疫相关基因表达分析

为探索鸡抗病育种中简便实用的免疫功能测定指标,试验采用成像流式细胞术对四川山地乌骨鸡和大恒699肉鸡配套系的4周龄和10周龄外周血进行T淋巴细胞亚群分型,并检测外周血中白介素6(IL-6)、Toll样受体3(TLR3)和干扰素(IFN-γ)基因的表达水平。结果显示:10周龄时,CD_(4)^(+)T淋巴细胞亚群在两鸡种间差异显著(P<0.05),4周龄和10周龄的两鸡种CD_(8)^(+)T淋巴细胞亚群均有极显著差异(P<0.01);CD_(4)^(+)/CD_(8)^(+)比值在0.7~1.6之间呈正态分布,且随周龄增加而降低;大恒699肉鸡配套系的CD_(4)^(+)/CD_(8)^(+)比值比四川山地乌骨鸡高;IL-6和IFN-γ表达量随周龄增加而增加,但TLR3相反。细胞因子的增多和CD_(4)^(+)/CD_(8)^(+)比值的降低表明鸡只随周龄增长,机体免疫平衡发生改变。结果表明,基于成像流式细胞术进行鸡外周血T淋巴细胞分型简易可行,CD_(4)^(+)/CD_(8)^(+)比值可作为鸡免疫力检测的一个指标;天然免疫相关基因IL-6、TLR3和IFN-γ基因的表达量可以作为免疫力检测的参考。

HIV感染者/AIDS患者96例抗病毒治疗前后CD_4^+T细胞水平分析

目的通过对HIV感染者/AIDS患者的高效抗逆转录病毒治疗(HARRT),观察CD4+T淋巴细胞绝对计数及CD4+T/CD8+T比值,分析抗病毒治疗的效果。方法检测治疗前后CD4+T淋巴细胞绝对计数及CD4+T/CD8+T比值,运用统计学方法对数据进行处理。结果 96例血标本抗病毒治疗前后CD4+T计数分别为(102.63±28.57)和(249.41±23.95)个/μl;CD4+T/CD8+T比值分别为(0.23±0.19)和(0.42±0.23),治疗效果明显,无论男性、女性治疗都是有效的;在无症状或出现较轻症状HIV感染者的治疗效果比疾病严重或较重者好。结论 HARRT的治疗效果明显,可提高HIV感染者/AIDS患者抗病毒治疗的比例,延缓疾病进程和降低病死率。

微波辐射损伤雷达作业人员外周血T淋巴细胞免疫活性

目的探讨微波辐射对雷达作业人员外周血T淋巴细胞免疫活性的影响。方法采取30例雷达作业人员和20例健康人的外周血,对外周血T淋巴细胞进行银染,检测淋巴细胞核仁银染强度;另外采用微量全血直接荧光法标记CD3+、CD4+、CD8+,用流式细胞仪检测T淋巴细胞亚群变化。结果巨嗜银核仁银染面积与细胞核银染面积的比值(I.S%),对照组为6.06±0.32,雷达作业组为5.12±0.57;对照组外周血CD3+、CD4+、CD8+百分比分别为(65.81±1.60)%、(32.58±0.71)%、(25.07±1.46)%,雷达作业组为(59.20±3.07)%、(28.32±2.66)%、(27.24±1.72)%。与对照组相比,雷达作业组外周血T淋巴细胞的I.S%明显降低(P<0.05),T淋巴细胞亚群CD3+、CD4+、CD4+/CD8+比值明显降低(P<0.05)。结论微波辐射对雷达作业人员的免疫活性产生明显影响,提示应加强雷达作业人员的防护措施。

CBP联合治疗重症急性胰腺炎合并多器官功能障碍综合征临床效果及对免疫功能、预后影响

目的探讨连续性血液净化(CBP)联合治疗重症急性胰腺炎合并多器官功能障碍综合征(MODS)的临床效果及对免疫功能、预后的影响。方法选取2018年7月—2021年11月收治的重症急性胰腺炎合并MODS 105例,按照治疗方案不同将其分为观察组(常规综合联合CBP治疗)54例和对照组(常规综合治疗)51例。比较2组临床疗效、炎性因子[C反应蛋白(CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]、免疫功能(CD^(4+)、CD8+和CD^(4+)/CD8+)及预后情况[感染率、病死率和急性生理与慢性健康评分量表Ⅱ(APACHEⅡ)评分]。结果治疗后7 d,观察组总有效率高于对照组(P<0.05)。治疗后1 d,CRP、IL-6和TNF-α水平2组均较治疗前降低,且观察组低于对照组;2组CD^(4+)和CD^(4+)/CD8+较治疗前升高,CD8+较治疗前降低;观察组CD^(4+)和CD^(4+)/CD8+高于对照组,CD8+低于对照组(P<0.05)。治疗后28 d,观察组APACHEⅡ评分低于对照组(P<0.05)。结论CBP联合治疗重症急性胰腺炎合并MODS效果显著,可降低炎性因子水平,改善免疫功能,且预后情况良好。

A Comparative Study on the Effect of BCG-PSN and Thymopeptides on T-lymphocyte Subsets of Normal and Immunosuppressed Mice

To compare the effects of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG PSN) and thymopeptides on T lymphocytes of normal and immunosuppressed mice, CD4 + and CD8 + T lymphocyte subsets of single nucleic cell in thymus, spleen and peripheral blood were detected successively by flow cytometry after application of BCG PSN and thymopeptides. Meanwhile, CD4 +/CD8 + ratio was also calculated. The results showed that both BCG PSN and thymopeptides could decrease the proportion of CD4 + CD8 + T lymphocyte subsets in the thymus, at the same time increase CD4 + T lymphocyte, CD8 +T lymphocyte proportion in the three tissues. The fluctuation in amplitude was greater in thymopeptides group than that in BCG PSN group. It is concluded that acting location of thymopeptides is in thymus, its stimulating action is stronger than that of BCG PSN, while BCG PSN not only accelerates the differentiation in thymus, but also has some direct stimulation to peripheral CD4 +T lymphocytes, and can maintain CD4 +/CD8 + ratio within normal range. So, BCG PSN is safer.

Disease Progression Patterns of SHIV-KB9 in Rhesus Macaques of Chinese Origin in Comparison with Indian Macaques

Objective To develop a model of SHIV-KB9/Chinese origin rhesus （Ch Rh） macaques for vaccine research and to compare the pathogenesis of SHIV-KB9 in Ch Rh macaques with that reported in Indian rhesus （Ind Rh） macaques. Methods Seven mamu-A＊01 negative Ch Rh macaques were inoculated intravenously with 1-10000 MID50 of SHIV-KB9. The monkeys were monitored for viral load, CD4, CDS, SHIV-specific antibody and virus genetic variation. The results were compared with those previously observed in Ind Rh macaques. Results As compared to that observed in Ind Rh macaques, SHIV-KB9 in Ch Rh macaques displayed three identical disease progression patterns. However, the primary pattern was not identical between the two subspecies. The level of plasma viremia differed in SHIV-KB9-infected Ch Rh macaques which exhibited different outcomes from those in Ind Rh macaques. Generally, the values of viral load and the maintenance of CD4^＋ T cells were associated with humoral responses. Otherwise, the viral genetic distances （divergence, diversity） were larger in animals （M419, M425） with their CD4^＋ T cells profoundly depleted. Conclusion The model of SHIV-KB9/Ch Rh macaques displays a relatively slow progression to AIDS compared with Ind Rh macaques, which may more accurately reflect the potential of candidate vaccines in humans.

肺癌患者外周血T淋巴细胞亚群检测的临床研究

通过流式细胞仪对确诊原发性支气管肺癌患者外周血T淋巴细胞亚群检测研究 ,并与健康人作对比。结果显示 ,肺癌患者CD3 + ( 68 88± 11 80 )和CD4+ ( 3 8 83± 10 3 0 )以及CD4+ /CD8+ ( 1 5 3± 0 74)比例均比正常人 ( 74 2 1±5 5 1,43 92± 7 0 2 ,1 89± 0 5 4)下降 ,CD8+ ( 2 9 2 6± 10 5 8)高于正常人 ( 2 4 5 7± 6 3 5 ) ,两组之间差异有统计学意义 ,P均 <0 0 5。肿瘤临床分期之间检测结果差异无统计学意义 ,而病理类型中未分化癌与鳞癌之间CD4+ 和CD4+ /CD8+下降具有统计学意义。初步研究结果提示 ,原发性支气管肺癌患者存在细胞免疫功能低下 ,并与细胞分化类型有关。

Epidemiological Aspects and Clinicopathological Findings in Cats Naturally Infected with Feline Leukemia Virus (FeLV) and/or Feline Immunodeficiency Virus (FIV)

Infections produced by feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), two of the most prevalent pathogens in cats, range from passing unnoticed to presenting a wide variety of clinical signs. Different epidemiological, clinical, hematological and virological parameters were analyzed in 78 FIV- and/or FeLV-infected cats. FeLV-infected (FeLV+) cats were considerably younger than FIV-infected (FIV+) cats, and in general were seen to have a more severe disease than FIV+ cats. Around one third of the cats presented anemia, and neutropenia was also frequently observed. Though a higher percentage of FIV+ than FeLV+ cats had altered leukocyte counts, FeLV+ cats had altered counts of both neutrophils and lymphocytes more frequently than FIV+, which usually presented only either altered neutrophils or lymphocyte counts. Virological markers were only detected in FeLV+cats, either as mono- or dual-infection, and correlated with the severity of the disease, but not in FIV+ cats. In conclusion, these results suggest that FeLV affects more blood cell types and provokes death of affected animals at a much earlier age than FIV and that the severity of the disease seemed to depend on the viral status of the cat.

The Hematopoietic and Immunomodulatory Effect of rhIL-12 for Liver Cancer

Purpose: To explore the effect of rhIL-12 on the number of the blood cells and CD4/8+ T, CD45+ leukocytes, and CD56+ NK cells in liver cancer patients following radiation therapy. Methods: We selected forty liver cancer patients who carried out by cyber knife (the patients were given 5 Gy every time for 5 times continuously) to observe the size of the tumor. After thirty hours, rhIL-12 was injected into the liver cancer patients via subcutaneous at the concentration of 50 ng/kg, 100 ng/kg, 200 ng/kg and 300 ng/kg in different patients, respectively. And there were ten patients in the four groups, respectively. The twenty patients who were selected from the hospital without rhIL-12 treatment were used as controls. All the blood cells were collected from different groups on day 0, hour 12, day 7, day 14, day 21 and day 28 after rhIL-12 treatment, respectively. The full number of blood cells in every group was analyzed by ELISA. The number of CD4/8+ T, CD45+ leukocytes, and CD56+ NK cells were detected by Flow Cytometry. After one month with rhIL-12 treatment, ECOG and WHO were used to evaluate the prognosis of liver cancer. Results: In present study, we found that the number of blood cells was significantly decreased on day 0 - day 3, while recovered from day 7 - day 14 and down-regulated on day 21 after rhIL-12 treatment. The number of CD4/8+ T, CD45+ leukocytes, and CD56+ NK cells was elevated with any concentration of rhIL-12. Furthermore, results showed that number of white blood cells was obviously higher than in patients without rhIL-12 treatment (P < 0.05). However, there was no significant difference of erythrocyte and platelet, between groups treated with rhIL-12 and control groups. In addition, the immune cells including CD4/8+ T, CD45+ leukocytes, and CD56+ NK cells were reduced on day 0 - day 3, recovered from day 7, and then decreased from day 21 in rhIL-12 treatment groups related to control groups (P < 0.05). Furthermore, studies showed that five patients developed symptoms of fever, bilirubin increased and liver dysfunction with the dose of 300 ng/kg. So we found that the safe and well-tolerated human dose of 200 ng/kg is within this efficacious range based on exposure parameters through the research. Higher ECOG and WHO scores were observed in rhIL-12 treatment groups compared to control groups (P = 0.025, P = 0.044, respectively). Conclusion: Our results suggested that rhIL-12 could recover the liver cancer induced aberrant blood cell number and CD4/8+ T, CD45+ leukocytes, and CD56+ NK cells , which may be an effective method to alleviate the progress of liver cancer and played an important role in treating liver cancer.