贲门癌患者外周血CD4^＋CD25^hiCD127^low调节性T细胞水平及其临床意义

目的 探讨贲门癌患者的免疫功能异常及其临床意义.方法 采用流式细胞术（FCM）和酶联免疫吸附（ELISA）方法分别检测56例贲门癌患者、15名健康人外周血中CD4^＋CD25^hiCD127^lowc调节性T细胞（Treg细胞）和血清IL-10、TGF-β1水平,结合临床资料进行分析.结果 56例贲门癌患者外周血中CD4^＋CD25^hiCD127lowTreg细胞占CD4^＋淋巴细胞的比例为（5.73±1.56）%,与健康对照组的（4.45±1.06）%相比,差异有统计学意义（P〈0.01）;血清中IL-10和TGF-β1含量均明显高于健康对照（P〈0.05）.贲门癌患者外周血CD4^＋CD25^hiCD127low Treg细胞水平与血清IL-10和TGF-β1含量呈正相关.贲门癌患者外周血CD4^＋CD25^hiCD127low Treg细胞数量与患者临床分期、淋巴结转移有关.结论 贲门癌患者外周血中CD4^＋CD25^hiCD127low Treg细胞表达增高,与临床分期有关,提示Treg水平异常与贲门癌的发生发展密切相关.

肺癌患者外周血CD4^＋CD25^HiCD127^Lo调节性T细胞检测的临床意义

目的探讨肺癌患者外周血中CD4^＋CD25^HiCD127^Lo调节性T细胞（Treg）占CD4^＋淋巴细胞比例的变化及其意义。方法采用流式细胞术检测肺癌患者30例（鳞癌20例、腺癌10例）及健康人（对照组）20名外周血中Treg的变化；ELISA法检测同一标本血清中自细胞介素10（IL-10），转化生长因子-β（TGF—β）的表达水平。结果鳞癌、腺癌患者外周血中Treg占CD4^＋T淋巴细胞的比例分别为（6．81±1．52）％、（7．08±1．17）％，两组间比较差异无统计学意义（P〉0．05），二者均高于对照组的（4．91±1．24）％，差异有统计学意义（P〈0．05）；肺癌组血清中IL—10水平为（24．36±4．02）ng／ml，高于健康对照组的（21．53±4．30）ng／ml；肺癌组血清中TGF—β水平为（218．49±35．23）ng／ml，高于健康对照组的（124．31±20．32）ng／ml。结论肺癌患者外周血中Treg比例明显升高，可能是导致免疫抑制的原因之一，并且可能通过分泌IL-10和TGF—β,发挥免疫抑制的效应。

新风胶囊对类风湿关节炎活动期伴贫血患者外周血CD4^+CD25^+CD127^(lo)调节性T细胞的影响

目的:探讨类风湿关节炎(RA)活动期伴贫血患者外周血CD4+CD25+CD127lo调节性T细胞(Treg)的变化及新风胶囊对其的影响。方法:40例RA活动期伴贫血的患者按随机数字表法分为新风胶囊组(XFC)和正清风痛宁组各20例,均1月1疗程,1疗程后观察疗效;同时设健康志愿者正常对照组20例。采用流式细胞仪检测三组外周血中CD4+CD25+CD127loTreg的数量及其在CD4+T淋巴细胞中所占的比例,并研究其与临床症状积分、疾病活动度指标(DAS28分值)、实验室指标之间的关系。结果:①与正常对照组相比,RA活动期伴贫血患者外周血CD4+CD25+CD127loTreg数量明显下降(P<0.01),活动期伴贫血患者及正常对照组外周血CD4+CD25+CD127loTreg占CD4+T淋巴细胞的百分比分别为(2.75±1.01)%和(4.18±0.89)%,前者明显低于后者(P<0.01)。②RA活动期伴贫血患者CD4+CD25+CD127loTreg表达水平与临床症状积分、DAS28评分、血沉(ESR)、C反应蛋白(CRP)、血小板(PLT)呈负相关(P<0.05),与血红蛋白(HGB)、血清铁(SI)及补体C3、C4呈正相关(P<0.05),而与类风湿因子(RF)、红细胞(RBC)、血清铁蛋白(SF)、转铁蛋白(TF)无明显相关性(P>0.05)。③两治疗组相比,XFC组在总有效率及改善关节症状及部分实验室指标方面与正清风痛宁组相似(P>0.05),但在上调CD4+CD25+CD127loTreg表达水平、降低DAS28分值及升高HGB、降低PLT数量方面显著优于正清风痛宁组(P<0.05)。结论:RA活动期伴贫血患者外周血中CD4+CD25+CD127loTreg表达水平低,并且与疾病活动度及贫血相关指标密切相关,提示该细胞参与了RA的发病和疾病的活动,同时也可能为RA引起贫血的原因之一,XFC可上调CD4+CD25+CD127loTreg表达水平,升高HGB、降低PLT数量,改善RA患者临床症状、改善贫血,可能为XFC治疗机制的重要方面。

Effects of estrogen on CD4^+ CD25^+ regulatory T cell in peripheral blood during pregnancy

Objective To investigate the effects of estrogen(E_2)level on regulatory T cells(Treg)in peripheral blood during pregnancy.Methods:A total of 30 healthy non-pregnant women were selected as control group,90 pregnant women of early,middle and late pregnancy and 30 postpartum women at 1 month after parturition were selected as experimental groups including early pregnancy group,middle pregnancy group and late pregnancy group;the proportions of CD4^+CD25^+Treg and CD4^+CD25^+CD127^-Treg among CD4 T cells were detected by flow cytometry;the serum estrogen content in peripheral blood was detected by electrochemical immune luminescence method.Results:E_2 level was coincident with the change of Tregs number during pregnancy.The estrogen content in peripheral blood increased gradually from early pregnancy to late pregnancy,then decreased significantly after parturition,and the level at 1 month after parturition down to the level in non-pregnancy group(P>0.05);the level of E_2 in pregnancy groups were significantly higher than those in non-pregnancy group(P<0.01);and there were significant differences among early pregnancy group,middle pregnancy group and late pregnancy group(P<0.05).The proportions of CD4^+CD25^+Treg and CD4^+CD25^+CD127^-Treg in pregnancy groups were significantly higher than those in non-pregnancy group(P<0.05),but decreased significantly after parturition,and there was no significant difference between non-pregnancy group and postpartum women group(P>0.05):the proportions in middle and late pregnancy groups were significantly higher than those in early pregnancy group(P<0.05).but decreased slightly in late pregnancy group,there was no significant difference between late pregnancy group and middle pregnancy group(P>0.05).There was correlation between Tregs number with estrogen level during pregnancy.The proportion of CD4^+CD25^+Treg and CD4^+CD25^+CD 127^-Treg were positively correlated with estrogen level.Conclusions:High proportion of CD4^+CD25^+Trcg and CD4^+CD25^+CD127^-Treg is closely related to the high level of E,during pregnancy.It suggested that high level of estrogen may induce an increase of CD4^+CD25^+Treg in peripheral blood.and then influence the immune function of pregnant women.The results of this experiment might play an important role of estrogen in immune-modulation during pregnancy.

大肠癌患者外周血CD_4^+CD_(25)^+CD_(127)^(lo/-)调节性T细胞的含量及临床意义

目的探讨大肠癌患者外周血CD4+、CD25+、CD127lo/-调节性T细胞(Treg)的变化及意义。方法流式细胞术检测36例大肠癌患者及30例健康人外周血中CD4+、CD25+、CD127lo/-Treg的变化;ELISA检测同一标本血清中转化生长因子β(TGF-β)的表达水平。结果36例大肠癌患者外周血中CD4+、CD25+、CD127lo/-Treg占CD4+、淋巴细胞的比例为(6.92±1.31)%,与正常对照组(4.91±1.24)%相比差异有显著性(P<0.05)。20例腺癌、7例黏液癌、9例未分化癌患者外周血中CD4+、CD25+、CD127lo/-Treg比例分别为(6.81±1.52)%、(6.99±1.21)%、(7.08±1.17)%,各组间比较差异无显著性(P>0.05),均高于对照组的(4.91±1.24)%,差异有显著性(P<0.05)。Ⅳ期大肠癌患者外周血CD4+、CD25+、CD127lo/-Treg高于Ⅱ期大肠癌患者(P<0.05)。大肠癌组外周血清中TGF-β水平显著高于正常对照组,随着TGF-β水平增加,CD4+、CD25+、CD127lo/-Treg比例逐渐增高,两者呈正相关。结论大肠癌患者外周血中CD4+、CD25+、CD127lo/-Treg表达增高,与临床分期相关,但与组织学分类无关,导致机体免疫抑制,可能参与大肠癌发生。

胃癌患者外周血CD4^+CD25^+CD127^(lo/-)调节性T细胞的表达及意义

目的:探讨胃癌患者外周血中CD4+CD25+CD127lo/-调节性T细胞(regulatory T cells,Treg)的表达及意义。方法:采用流式细胞术检测26例胃癌患者、30例胃溃疡患者和20例健康志愿者外周血中CD4+CD25+CD127lo/-Treg、细胞毒性T细胞(cytotoxic T lympho-cytes,CTL)和NK细胞,采用ELISA法检测血清中γ-干扰素(interferon γ,IFN-γ)的表达水平。结果:胃癌患者外周血中CD4+CD25+CD127lo/-Treg占CD4+T淋巴细胞的百分比为(6.12±2.08)%,高于胃溃疡患者和健康志愿者(P均<0.05)。胃癌患者外周血中CD4+CD25+CD127lo/-Treg水平与CTL、NK细胞及IFN-γ呈负相关。结论:胃癌患者外周血中具有免疫抑制活性的CD4+CD25+CD127lo/-Treg水平较高,对胃癌患者具有肿瘤免疫抑制作用。

强直性脊柱炎患者外周血CD4^＋调节性T细胞的变化及意义

目的探讨强直性脊柱炎（ankylosing spondylitis，AS）患者外周血中CD4^＋调节性T细胞（regulatory T cells，Treg）的表达、功能及意义。方法采用流式细胞术检测78例As患者和50例健康志愿者外周血中CD4^＋CD25^＋CD127^lo/-Treg、细胞毒性T细胞（cytotoxic T lymphocytes，CTL）和NK细胞，采用ELISA法检测血清中B型转化生长因子（transforming growth factor-β，TGF-β）和肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的表达水平。从患者外周血单个核细胞中磁珠分选出CD4^＋CD25^＋Treg细胞，混合淋巴细胞培养（mixedlymphocyteculture，MLC）分析其免疫抑制功能。结果AS活动期患者外周血中CD4^＋CD25^＋CD127^lo-Treg占CD4^＋T淋巴细胞的百分比为（4．36±1．21）％，MLC中CD4^＋CD25^＋Treg抑制同种异体T淋巴细胞增殖功能低下，与其Treg分泌TGF-β减少相关，CD4^＋Treg含量及功能均低于健康志愿者（P〈0．05）。AS患者外周血中CD4^＋CD25^＋CD127^lo/-Treg水平与TGF—β呈正相关，与TNF-α呈负相关。结论AS患者外周血中Treg表达水平低，且存在功能缺陷，导致体内诱导免疫耐受机能不足，可能参与AS免疫发病。