中药调控CD^(+)_(4)T细胞干预湿疹炎症应答的研究进展

湿疹是一种变态反应性炎症性皮肤病,近期研究表明以CD^(+)_(4)T细胞亚群如Th1/Th2、Th17/调节性T细胞(regulatory T cells,Treg)细胞分化失衡导致湿疹发病中出现过度炎症应答,而中药以改善CD^(+)_(4)T细胞亚群分化平衡干预湿疹免疫微环境具有较好的调节作用。以湿疹炎症应答前沿研究动态,探讨中药在改善调控CD^(+)_(4)T细胞亚群分化干预湿疹致病的作用优势,为中药在改善湿疹临床症状及机制研究提供一定的理论指导。

Nocardia cyriacigeorgica infection in a patient with repeated fever and CD4+T cell deficiency:A case report

BACKGROUND Nocardia pneumonia shares similar imaging and clinical features with pulmonary tuberculosis and lung neoplasms,but the treatment and anti-infective medication are completely different.Here,we report a case of pulmonary nocardiosis caused by Nocardia cyriacigeorgica(N.cyriacigeorgica),which was misdiagnosed as community-acquired pneumonia(CAP)with repeated fever.CASE SUMMARY A 55-year-old female was diagnosed with community-acquired pneumonia in the local hospital because of repeated fever and chest pain for two months.After the anti-infection treatment failed in the local hospital,the patient came to our hospital for further treatment.Enhanced computed tomography showed multiple patchy,nodular and strip-shaped high-density shadows in both lungs.A routine haematological examination was performed and showed abnormalities in CD19+B cells and CD4+T cells.Positive acid-fast bifurcating filaments and branching gram-positive rods were observed in the bronchoalveolar lavage fluid of the patient under an oil microscope,which was identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry as N.cyriacigeorgica.The patient’s condition quickly improved after taking 0.96 g compound sulfamethoxazole tablets three times a day.CONCLUSION The antibiotic treatment of Nocardia pneumonia is different from that of common CAP.Attention should be given to the pathogenic examination results of patients with recurrent fever.Nocardia pneumonia is an opportunistic infection.Patients with CD4+T-cell deficiency should be aware of Nocardia infection.

Efficient Numerical Scheme for the Solution of HIV Infection CD4^(+)T-Cells Using Haar Wavelet Technique

In this paper,Haar collocation algorithmis developed for the solution of first-order ofHIV infection CD4^(+)T-Cells model.In this technique,the derivative in the nonlinear model is approximated by utilizing Haar functions.The value of the unknown function is obtained by the process of integration.Error estimation is also discussed,which aims to reduce the error of numerical solutions.The numerical results show that the method is simply applicable.The results are compared with Runge-Kutta technique,Bessel collocation technique,LADM-Pade and Galerkin technique available in the literature.The results show that the Haar technique is easy,precise and effective.

CD4+ T-Cell Count, Serum Zinc, Copper and Selenium Levels in HIV Sero-Positive Subjects on ART and ART Naïve Subjects in Port Harcourt, Nigeria

Background: HIV infection results in depletion of immunocompetent cells such as CD4+ T-cells. Trace elements such as Copper, Zinc and selenium are known to be involved in immune function. In recent times, HIV-positive patients are treated with antiretroviral therapy (ART), with significant progress. This study was aimed at evaluating CD4+ T-cells levels, serum Copper, Zinc and Selenium levels in HIV seropositive subjects on ART and ART naive subjects (HIV positive subjects that have not started ART treatment) in Rivers State, Nigeria. Methods: 150 subjects aged 20 to 79 years were recruited after informed consent. 70 subjects were HIV-positive on ART, 30 subjects were HIV-positive ART naïve subjects, while 50 subjects were apparently healthy subjects. Ten (10) milliliters of blood was collected using a standard venipuncture technique from each subject for the analysis of CD4 T-cells using BD fluorescent activated cell sorter (FACSC count), serum Copper and Zinc were analyzed colorimetrically using semi auto-analyzer WP 21E, while selenium was analyzed using atomic absorption spectrophotometer ELICO, SL173. Data generated were analyzed using Graph-Pad Prism version 8.0.2 and p Result: This study revealed a significant reduction in mean zinc, selenium and CD4+ T-cell level respectively (p = 0.0006;0.0001;0.0001) in HIV-Positive subjects on ART and ART naive. There was also a significant increase in mean serum copper level in the HIV-positive subject as compared to control subjects (p = 0.0001). ART treatment improved the CD4+ T cell count and serum levels of selenium and zinc;however, ART did not correct the imbalance. Furthermore, female subjects on ART have a significantly higher CD4+ T-cell count than the males (p Conclusion: Selenium and Zinc deficiency are associated with HIV disease despite the role of ART hence micronutrient supplementation is advised for HIV-positive subjects on ART.

子宫内膜异位症患者T细胞亚群CD_4、CD_8的测定

目的 :通过检测子宫内膜异位症患者T淋巴细胞亚群 ,了解CD4 和CD8T细胞及其CD4 /CD8比值与正常对照之间的改变 ,探讨子宫内膜异位症的免疫病理变化。方法 :采用荧光激活细胞分析仪 (FACS) ,检测外周血T细胞亚群CD4 和CD8的比例及CD4 /CD8比值。结果 :子宫内膜异位症组外周血CD4 比例高于对照组 ,CD8比例低于对照组 ,CD4 /CD8比值高于对照组 ,各组间差异有显著性 (P <0 .0 0 1)。结论 :子宫内膜异位症患者免疫调节功能紊乱 ,辅助性T细胞功能增强而抑制性T细胞功能降低 ,淋巴细胞过度活化 ,使子宫内膜细胞脱落。

CD4^(+)T cells produce IFN-I to license cDC1s for induction of cytotoxic T-cell activity in human tumors

CD4^(+)T cells can"help"or"license" conventional type 1 dendritic cells(cDC1s)to induce CD8^(+)cytotoxic T lymphocyte(CTL)anticancer responses,as proven in mouse models.We recently identified cDC1s with a transcriptomic imprint of CD4^(+)T-cell help,specifically in T-cell-infiltrated human cancers,and these cells were associated with a good prognosis and response to PD-1-targeting immunotherapy.Here,we delineate the mechanism of cDC1 licensing by CD4^(+)T cells in humans.Activated CD4^(+)T cells produce IFNβvia the STING pathway,which promotes MHC-I antigen(cross-)presentation by cDC1s and thereby improves their ability to induce CTL anticancer responses.In cooperation with CD40 ligand(L),IFNβalso optimizes the costimulatory and other functions of cDC1s required for CTL response induction.IFN-I-producing CD4^(+)T cells are present in diverse T-cell-infiltrated cancers and likely deliver“help”signals to CTLs locally,according to their transcriptomic profile and colocalization with“helped/licensed”cDCs and tumor-reactive CD8^(+)T cells.In agreement with this scenario,the presence of IFN-I-producing CD4^(+)T cells in the TME is associated with overall survival and the response to PD-1 checkpoint blockade in cancer patients.

颗粒蛋白前体衍生物Atsttrin对骨关节炎的保护作用及对CD^(+)_(4)T细胞的调节作用

目的揭示颗粒蛋白前体衍生物Atsttrin对骨关节炎的保护作用及对CD^(+)_(4)T细胞的调节作用。方法本研究起止时间为2018年10月至2019年12月,60只5周龄SD雄性大鼠购自西安交通大学医学部实验动物中心。采用膝关节前交叉韧带横断法建立骨关节炎大鼠模型,通过关节内注射15μL Atsttrin(1μg/μL)对模型大鼠进行治疗,每周注射1次,连续注射4周。通过番红O固绿染色检测软骨损伤程度,采用OARSI评分系统评价软骨退化情况。ELISA法检测大鼠血清干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)、白细胞介素-17(IL-17)和转化生长因子-β(TGF-β)水平。流式细胞仪分析血清Th1、Th2、Th17和Treg细胞比例。应用终浓度为10μg/L的肿瘤坏死因子-α(TNF-α)诱导滑膜细胞和软骨细胞48 h,并分别用终浓度为10μg/L、20μg/L、50μg/L的Atsttrin处理滑膜细胞和软骨细胞48 h。通过5-溴-2-脱氧尿苷(BrdU)细胞增殖测定试剂盒检测滑膜或软骨细胞的增殖。通过RT-PCR和Westernblotting检测软骨细胞中解聚蛋白样金属蛋白酶-4(ADAMTS-4)、基质金属蛋白酶-13(MMP-13)、IFN-γ、IL-4、IL-17和TGF-β的信使核糖核酸(mRNA)和蛋白表达。结果与模型组相比,Atsttrin组大鼠的膝关节软骨破坏情况明显减轻(P<0.05)。与模型组相比,Atsttrin组大鼠国际骨关节炎研究学会(OARSI)评分显著降低[(2.56±0.21)比(1.08±0.11),P=0.001]。Atsttrin组大鼠的血清IFN-γ和IL-17水平显著低于模型组,而IL-4和TGF-β水平显著高于模型组(P<0.05)。Atsttrin组大鼠的血清Th1和Th17细胞比例显著低于模型组,而Th2和Treg细胞比例显著高于模型组(P<0.05)。Atsttrin处理显著降低了TNF-α诱导的滑膜细胞的增殖能力,并提高了TNF-α诱导的软骨细胞的增殖能力(P<0.05)。Atsttrin显著下调了TNF-α诱导的软骨细胞中ADAMTS-4、MMP-13、IFN-γ和IL-17的mRNA和蛋白表达水平,并上调了IL-4和TGF-β的表达水平(P<0.05)。结论Atsttrin可有效抑制骨关节炎大鼠模型的软骨病变并提高关节功能。Atsttrin可通过抑制滑膜细胞增殖、促进软骨细胞增殖、抑制软骨机制降解来维持关节内微环境的稳定。此外,Atsttrin的关节软骨保护作用与纠正Th1/Th2细胞以及Th17/Treg细胞的失衡有关。

Advanced Glycation End Products Promote Differentiation of CD4^+ T Helper Cells toward Pro-inflammatory Response

This study investigated the effect of advanced glycation end products（AGEs） on differentiation of na ve CD4＋T cells and the role of the receptor of AGEs（RAGE） and peroxisome proliferator-activated receptors（PPARs） activity in the process in order to gain insight into the mechanism of immunological disorders in diabetes. AGEs were prepared by the reaction of bovine serum albumin（BSA） with glucose. Human na ve CD4＋T cells, enriched from blood of healthy adult volunteers with negative selection assay, were cultured in vitro and treated with various agents including AGEs, BSA, high glucose, PGJ2 and PD68235 for indicated time. In short hairpin（sh） RNA knock-down experiment, na ve CD4＋T cells were transduced with media containing shRNA-lentivirus generated from lentiviral packaging cell line, Lent-XTM293 T cells. Surface and intracellular cytokine stainings were used for examination of CD4＋T cell phenotypes, and real-time PCR and Western blotting for detection of transcription factor mRNA and protein expression, respectively. The suppressive function of regulatory T（Treg） cells was determined by a [3H]-thymidine incorporation assay. The results showed that AGEs induced higher pro-inflammatory Th1/Th17 cells differentiated from na ve CD4＋T cells than the controls, whereas did not affect anti-inflammatory Treg cells. However, AGEs eliminated suppressive function of Treg cells. In addition, AGEs increased RAGE mRNA expression in na ve CD4＋T cells, and RAGE knock-down by shRNA eliminated the effect of AGEs on the differentiation of CD4＋T cells and the reduction of suppressive function of Treg cells. Furthermore, AGEs inhibited the mRNA expression of PPARγ, not PPARα; PPARγ agonist, PGJ2, inhibited the effect of AGEs on na ve CD4＋T cell differentiation and reversed the AGE-reduced suppressive function of Treg cells; on the other hand, PPARγ antagonist, PD68235, attenuated the blocking effect of RAGE shRNA on the role of AGEs. It was concluded that AGEs may promote CD4＋T cells development toward pro-inflammatory state, which is associated with increased RAGE mRNA expression and reduced PPARγ activity. ＋

CD4^+CD25^(high) Regulatory Cells in Peripheral Blood of NSCLC Patients

The proportion and changes of CD4^＋CD25^high regulatory T cells （Trs） in peripheral blood of non-small cell lung cancer （NSCLC） patients were analyzed and their clinical significance explored. The peripheral blood was collected from 61 patients with NSCLC and 15 healthy controls. By using monoclonal antibodies, the blood samples were evaluated with the flow cytometry for lymphocyte subsets （CD3^＋, CD4^＋ and CD8^＋） and CD4^＋CD25^high Tr cells. The results showed that the proportion of CD4^＋CD25^high Tr cells in NSCLC group was significantly higher than in control group [（4.36 ±2.07） % vs （2.04±1.03） %, P〈0.01]. The proportion of CD4^＋CD25^ high Tr cells in late stage was higher than that in early stage [stages Ⅰ ＋Ⅱ （2.264±0.6） %; stage Ⅲ（3.284± 1.38） %; stage IV （6.06 4±4.08） %] （P〈0.05）. Kaplan-Meier survival analysis revealed that the prognosis of the patients who had higher proportion of CD4^＋CD25^high Tr cells in peripheral blood was worse （P=0.0026）. In conclusion, the relative increase in CD4^＋CD25^high Tr cells in peripheral blood may be related to im- munosuppression and tumor progression in patients with NSCLC. This finding suggests that CD4^＋CD25^high Tr cells in peripheral blood of NSCLC may be positive for prognosis analysis. The use of depletion of the CD4^＋CD25^high Tr cell therapy to treat NSCLC patients may be an effective strategy.

CD4-positive diffuse large B-cell lymphoma:A variant with aggressive clinical potential

CD4 expression is rare in diffuse large B-cell lymphoma(DLBCL), with 4 previously reported cases. Its significance is uncertain. We report five patients with CD4+ DLBCL and one CD4+ primary mediastinal large B-cell lymphoma. Cases were identified by searching the electronic database of the department; each was reviewed. Average age was 56 years. Neoplastic cells expressed CD20(5/6 tested cases). BCL2/BCL6 expression were seen in 3/3 tested cases, suggesting a germinal center origin. Additionally, expression of T-cell antigens CD2 and CD5 was noted in 2/2 and CD7 in 1/1 tested case. CD3 was negative in all. Lymph nodes were commonly involved(67%). Patients received chemotherapy +/- radiation(6/6) and bone marrow transplant(2/6). Average survival was 44.2 mo. CD4 expression in DLBCL raises questions of lineage commitment. CD4+ DLBCL is rare; care should be exercised not to diagnose these as T-cell lymphomas. A subset behaves aggressively.

Baseline Naive CD4＋ T-cell Level Predicting Immune Reconstitution in Treated HIV-infected Late Presenters

Background：Among HlV-infected patients initiating antiretroviral therapy （ART）,early changes in CD4＋ T-cell subsets are well described.However,HIV-infected late presenters initiating treatment present with a suboptimal CD4＋ T-cell reconstitution and remain at a higher risk for AIDS and non-AIDS events.Therefore,factors associated with CD4＋ T-cell reconstitution need to be determined in this population,which will allow designing effective immunotherapeutic strategies.Methods：Thirty-one adult patients with baseline CD4＋ T-cell count 〈350 cells/mm^3 exhibiting viral suppression after ART initiation were followed in the HIV/AIDS research center of Peking Union Medical College Hospital in Beijing,China,from October 2002 to September 2013.Changes in T-cell subsets and associated determinants were measured.Results：Median baseline CD4＋ T-cell count was 70 cells/mm3.We found a biphasic reconstitution ofT-cell subsets and immune activation：a rapid change during the first 6 months followed by a more gradual change over the subsequent 8 years.Baseline CD4＋ T-cell count 〉200 cells/ mm3 in comparison to CD4＋ T-cell count ≤200 cells/mm3 was associated with more complete immune Reconstitution （77.8% vs.27.3% respectively;P =0.017） and normalized CD4/CD8 ratio.We showed that the baseline percentage of naive CD4＋ T-cell was a predictive marker for complete immune reconstitution （area under receiver operating characteristic curve 0.907）,and 12.4% as cutoffvalue had a sensitivity of 84.6% and a specificity of 88.2%.Conclusions：Baseline naive CD4＋ T-cell percentage may serve as a predictive marker for optimal immune reconstitution during long-term therapy.Such study findings suggest that increasing thymic output should represent an avenue to improve patients who are diagnosed late in the course of infection.

CD4^＋ T-cell dependence of primary CD8^＋ T-cell response against vaccinia virus depends upon route of infection and viral dose

CD4^＋ T-cell help （CD4 help） plays a pivotal role in CD8^＋ T-cell responses against viral infections. However, the role in primary CD8^＋ T-cell responses remains controversial. We evaluated the effects of infection route and viral dose on primary CD8^＋ T-cell responses to vaccinia virus （VACV） in MHC class II^-/- mice. CD4 help deficiency diminished the generation of VACV-specific CD8^＋ T cells after intraperitoneal （i.p.） but not after intranasal （i.n.） infection. A large viral dose could not restore normal expansion of VACV-specific CD8^＋ T cells in i.p. infected MHC II-/- mice. In contrast, dependence on CD4 help was observed in i.n. infected MHC II-/- mice when a small viral dose was used. These data suggested that primary CD8~ T-cell responses are less dependent on CD4 help in i.n. infection compared to i.p. infection. Activated CD8~ T cells produced more I FN-y, TNF-a and granzyme B in i.n. infected mice than those in i.p. infected mice, regardless of CD4 help. IL-2 signaling via CD25 was not necessary to drive expansion of VACV-specific CD8~ T cells in i.n. infection, but it was crucial in i.p. infection. VACV-specific CD8^＋ T cells underwent increased apoptosis in the absence of CD4 help, but proliferated normally and had cytotoxic potential, regardless of infection route. Our results indicate that route of infection and viral dose are two determinants for CD4 help dependence, and intranasal infection induces more potent effector CD8^＋ T cells than i.D. infection.

Early life undernutrition reprograms CD4^+T-cell glycolysis and epigenetics to facilitate asthma

With the support by the National Natural Science Foundation of China,the research team directed by Prof.Huang HeFeng(黄荷风)at Shanghai Key Laboratory of Embryo Original Disease,Institute of Embryo Fetal Original Adult Disease and International Peace Maternity&.Child Health Hospital,School of Medicine,Shanghai Jiao Tong University.

Parameters estimation of HIV infection model of CD4＋ T-cells by applying orthonormal Bernstein collocation method

The HIV infection model of CD4＋ T-cells corresponds to a class of nonlinear ordinary differential equation systems. In this study, we provide the approximate solution of this model by using orthonormal Bernstein polynomials （OBPs）. By applying the proposed method, the nonlinear system of ordinary differential equations reduces to a nonlinear system of algebraic equations which can be solved by using a suitable numerical method such as Newton＇s method. We prove some useful theorems concerning the convergence and error estimate associated to the present method. Finally, we apply the proposed method to get the numerical solution of this model with the arbitrary initial conditions and values. Furthermore, the numerical results obtained by the suggested method are compared with the results achieved by other previous methods. These results indicate that this method agrees with other previous methods.

A numerical method for the solutions of the HIV infection model of CD4＋ T-cells

In this paper, the human immunodeficiency virus （HIV） infection model of CD4＋ T-cells is considered. In order to numerically solve the model problem, an operational method is proposed. For that purpose, we construct the operational matrix of integration for bases of Taylor polynomials. Then, by using this matrix operation and approximation by polynomials, the HIV infection problem is transformed into a system of algebraic equations, whose roots are used to determine the approximate solutions. An important feature of the method is that it does not require collocation points. In addition, an error estimation technique is presented. We apply the present method to two numerical examples and compare our results with other methods.

Effect of MTECl on the functional expression of TCRαβ^+3G11^-6C10^-CD4^+CD8^- thymocyte subset

A murine CD4+ thymocyte subset with phenotype of TCRαβ + 3G11- 6C10- CD4 + CD8- CD69 + /- HSAmed/lo contains the cells in relatively functional matured status. The functional property of the cells in this subset is characterized by the unique pattern of cytokine production at transitional stage from ThO to Th2 type with the latter being the dominant type. After being co-cultured with murine thymic medullary epithelial cell line (MTEC1) cells, a murine

复发性流产患者外周血T淋巴细胞亚群及CD_4^+辅助性T淋巴细胞亚型的变化

目的 探讨不明原因的复发性流产 (URSA)与T淋巴细胞免疫功能的关系。方法 采用酶联免疫斑点 (ELISPOT)法对 30例URSA患者和 2 4例正常妇女 (对照 )的外周血T淋巴细胞亚群及CD+4 辅助性T淋巴细胞 (Th细胞 )亚型进行检测。结果 与对照相比 ,URSA患者外周血中CD+4 辅助性T淋巴细胞百分率增多 (分别为 45 %、6 0 % ,P <0 0 5 ) ,Th1细胞的百分率升高 (14%与 2 6 % ,P<0 0 1) ,Th2细胞的百分率降低 (2 0 %与 7% ,P <0 0 5 ) ,Th1/Th2细胞比值增大 (0 73与 3 80 ,P<0 0 1)。结论 URSA的发生可能与CD+4 辅助性T淋巴细胞的增多及Th1细胞介导的细胞免疫功能的增强有关。

应用流式细胞仪研究深圳地区健康成人及HIV/AIDS患者的免疫状况

目的 :研究深圳地区健康人、HIV无症状感染者及AIDS患者的免疫状态 ,以了解艾滋病早期T细胞亚群的变化过程。方法 :应用流式细胞仪三色荧光单克隆抗体 (CD4-FITC/CD8-PE/CD3 -PC5 )检测 3 4例正常健康成人 ,1 2例HIV感染无症状者 ,2 3例AIDS患者的外周血CD+ 4 、CD+ 8T淋巴细胞计数及CD+ 4 /CD+ 8比值。结果 :HIV感染无症状者及AIDS患者的CD+ 4 淋巴细胞及CD+ 4 /CD8比值均明显低于健康成人 (P <0 0 1 ) ;HIV感染无症状者CD+ 8T淋巴细胞明显高于正常健康人和AIDS患者 (P <0 0 1 ) ;CD+ 4 T淋巴细胞随病程进展不断下降 ,AIDS患者与HIV感染无症状者之间存在着显著差异。结论 :T淋巴细胞免疫状况可作为评价AIDS病程进展程度的重要指标。

乙型肝炎的T淋巴细胞亚群的演变规律

目的:了解不同类型乙型肝炎患者T淋巴细胞亚群的演变规律及意义。方法:我院2002年7月～2004年6月收治的16例急性肝炎、40例慢性肝炎及46例重型肝炎病例的抗凝血,通过流式细胞仪检测其T淋巴细胞亚群,对不同类型肝炎进行统计学分析。结果:慢性肝炎组与急性肝炎组比较CD3+,CD8+细胞计数降低且差异有显著性(P<0.05)。重型肝炎组与非重型肝炎组(急性肝炎组与慢性肝炎组)比较CD3+,CD4+,CD8+细胞计数明显降低(P<0.05),幼稚的CD4+,CD8+细胞计数明显升高(P<0.05)。结论:不同类型乙型肝炎的T淋巴细胞亚群有明显差异,乙型肝炎随着病情严重及病程延长,CD3+,CD4+,CD8+淋巴细胞逐渐减少,重型肝炎出现大量幼稚的CD4+CD8+淋巴细胞,T淋巴细胞亚群的变化可以反映病情严重程度及免疫状态,对免疫调节治疗有指导意义。

康艾注射液联合放疗治疗非小细胞肺癌随机平行对照研究

[目的]观察康艾注射液联合放疗治疗非小细胞肺癌疗效。[方法]使用随机平行对照方法,将76例住院患者按住院病志号简单随机分为两组。对照组34例常规放射治疗,射野包括原发灶、同侧肺门、纵膈隆突下淋巴结、引流区,若病灶单一,且位于边缘,则照射病灶区及病灶外扩1.0~1.5cm区域,有明显淋巴结肿大区域,也包括在射野内6mv-X射线2ay/f DT36gy后射野避开脊髓,总剂量DT60GY^70GY。治疗组42例康艾注射液10 mL/支,40~60 mL+0.9%生理盐水,静滴,1次/d;放疗同对照组。治疗30d为1疗程。观测临床症状、T淋巴细胞亚群(CD+3 CD+4 CD+8 CD+4/CD+8)、卡式评分、不良反应(白细胞降低,体重下降,乏力改善以及伴发症状疼痛、低热、胸闷、食欲不振等)。治疗1疗程,随访四周,判定疗效。[结果]瘤体消退治疗组优于对照组(P<0.05);相关指标(体重、白细胞、乏力、生活质量,疼痛、低热、胸闷、食欲不振)治疗组改善优于对照组(P<0.05),生活质量,疼痛、低热、胸闷、食欲不振等改善两组间无明显差异(P>0.05);卡氏评分对照组明显降低(P<0.05),治疗组无明显变化(P>0.05),治疗组降低幅度低于对照组(P<0.05);T细胞亚群(CD+3CD+4 CD+8 CD+4/CD+8)治疗组无明显变化(P>0.05),对照组明显降低(P<0.01),对照组优于治疗组(P<0.01)。[结论]康艾注射液联合放疗治疗非小细胞肺癌,可明确减轻毒副作用、缓解不适症状、改善生活质量,疗效满意,值得推广。