目的探讨小鼠心肌梗死进程中巨噬细胞亚群的动态变化与CD59、P2X7表达的动态变化。方法将成年雄性C57BL/6J小鼠36只随机分为对照组(不做任何处理,6只)和心肌梗死组(30只)。通过冠状动脉左前降支结扎建立小鼠急性心肌梗死模型,心肌梗死...目的探讨小鼠心肌梗死进程中巨噬细胞亚群的动态变化与CD59、P2X7表达的动态变化。方法将成年雄性C57BL/6J小鼠36只随机分为对照组(不做任何处理,6只)和心肌梗死组(30只)。通过冠状动脉左前降支结扎建立小鼠急性心肌梗死模型,心肌梗死组30只小鼠又随机分为A、B、C、D、E组,分别在心肌梗死后1、3、5、7、14 d取材。收取小鼠的外周血和心脏组织,采用流式细胞术检测巨噬细胞亚群的动态变化与CD59和P2X7的表达情况。结果心肌梗死后1、3 d外周血中单核细胞数量均高于对照组[(9.5±1.7)、(10.3±3.7)×10^(4)/ml比(3.2±3.1)×10^(4)/ml](均P<0.05)。心肌梗死后1、3、5 d心脏组织白细胞计数、巨噬细胞计数均高于对照组(均P<0.05)。心脏组织中Ly6C^(high)巨噬细胞计数在心肌梗死后3 d达到高峰,而Ly6C^(low)亚群在心肌梗死后5 d达到高峰,均高于对照组(均P<0.05)。心肌梗死后1 d P2X7^(-)CD59^(-)巨噬细胞占Ly6C^(high)巨噬细胞百分比高于对照组,而P2X7^(+)CD59^(+)巨噬细胞占比低于对照组(均P<0.05)。心肌梗死后1、3、5 d P2X7^(+)CD59^(+)巨噬细胞占Ly6C^(low)巨噬细胞百分比均低于对照组,而P2X7^(-)CD59^(+)巨噬细胞占比均高于对照组(均P<0.05)。结论在小鼠急性心肌梗死的不同阶段,心脏组织巨噬细胞的亚群呈现动态变化,同时伴随CD59和P2X7表达的动态变化。展开更多
Objective To detection the expression of membrane associated complement regulatory proteins(CRP) CD55 and CD59 on the major peripheral blood cells(erythrocytes and neutrophils) in patients with paroxysmal nocturnal he...Objective To detection the expression of membrane associated complement regulatory proteins(CRP) CD55 and CD59 on the major peripheral blood cells(erythrocytes and neutrophils) in patients with paroxysmal nocturnal hemoglobinuria(PNH),aplastic anemia,microcytic hypochromic anemia,macrocytic anemia,oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia, and analysis its clinical implications.Methods Using fluorchrome (PE and FITC) labeled anti CD55 and anti CD59 monoclonal antibodies, and analyzing with flow cytometry.Results All the ratios of CD55 - and CD59 - cells in 35 normal peoples are less than 5%,but in excess of 40% in the 5 PNH patients.The results of Some patients with aplastic anemia are more than 5%, but less than 15%. About 50% patientswith microcytic hypochromic anemia having CD55 - and CD59 - erythrocytes aremore than 5%(less than 15%),but their neutrophils are normal.The results of patients with macrocytic anemia; oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia are no more than 5%.Conclusion Detection of CD55 - and CD59 - erythrocytes and neutrophils in the peripheral blood is avaluable and sensitive test in diagnosis and differential diagnosis of PNH, aplastic anemia and microcytic hypochromic anemia, and it can be used to evaluate the curative effect of PNH.展开更多
文摘目的探讨小鼠心肌梗死进程中巨噬细胞亚群的动态变化与CD59、P2X7表达的动态变化。方法将成年雄性C57BL/6J小鼠36只随机分为对照组(不做任何处理,6只)和心肌梗死组(30只)。通过冠状动脉左前降支结扎建立小鼠急性心肌梗死模型,心肌梗死组30只小鼠又随机分为A、B、C、D、E组,分别在心肌梗死后1、3、5、7、14 d取材。收取小鼠的外周血和心脏组织,采用流式细胞术检测巨噬细胞亚群的动态变化与CD59和P2X7的表达情况。结果心肌梗死后1、3 d外周血中单核细胞数量均高于对照组[(9.5±1.7)、(10.3±3.7)×10^(4)/ml比(3.2±3.1)×10^(4)/ml](均P<0.05)。心肌梗死后1、3、5 d心脏组织白细胞计数、巨噬细胞计数均高于对照组(均P<0.05)。心脏组织中Ly6C^(high)巨噬细胞计数在心肌梗死后3 d达到高峰,而Ly6C^(low)亚群在心肌梗死后5 d达到高峰,均高于对照组(均P<0.05)。心肌梗死后1 d P2X7^(-)CD59^(-)巨噬细胞占Ly6C^(high)巨噬细胞百分比高于对照组,而P2X7^(+)CD59^(+)巨噬细胞占比低于对照组(均P<0.05)。心肌梗死后1、3、5 d P2X7^(+)CD59^(+)巨噬细胞占Ly6C^(low)巨噬细胞百分比均低于对照组,而P2X7^(-)CD59^(+)巨噬细胞占比均高于对照组(均P<0.05)。结论在小鼠急性心肌梗死的不同阶段,心脏组织巨噬细胞的亚群呈现动态变化,同时伴随CD59和P2X7表达的动态变化。
文摘Objective To detection the expression of membrane associated complement regulatory proteins(CRP) CD55 and CD59 on the major peripheral blood cells(erythrocytes and neutrophils) in patients with paroxysmal nocturnal hemoglobinuria(PNH),aplastic anemia,microcytic hypochromic anemia,macrocytic anemia,oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia, and analysis its clinical implications.Methods Using fluorchrome (PE and FITC) labeled anti CD55 and anti CD59 monoclonal antibodies, and analyzing with flow cytometry.Results All the ratios of CD55 - and CD59 - cells in 35 normal peoples are less than 5%,but in excess of 40% in the 5 PNH patients.The results of Some patients with aplastic anemia are more than 5%, but less than 15%. About 50% patientswith microcytic hypochromic anemia having CD55 - and CD59 - erythrocytes aremore than 5%(less than 15%),but their neutrophils are normal.The results of patients with macrocytic anemia; oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia are no more than 5%.Conclusion Detection of CD55 - and CD59 - erythrocytes and neutrophils in the peripheral blood is avaluable and sensitive test in diagnosis and differential diagnosis of PNH, aplastic anemia and microcytic hypochromic anemia, and it can be used to evaluate the curative effect of PNH.
