The presence of a relatively mature CD4+CD8- (SP)T cell subset in mouse thymus has been demonstrated. Composing of 10% of total CD4SP thymocytes, this subset is defined by the absence of 3G11 and 6C10 expression with ...The presence of a relatively mature CD4+CD8- (SP)T cell subset in mouse thymus has been demonstrated. Composing of 10% of total CD4SP thymocytes, this subset is defined by the absence of 3G11 and 6C10 expression with a phenotype of CD69 +/- , HSAmed/lo and heterogeneous for Qa - 2 expression. The proliferation capability of TCRαβ+ 3G11-6C10- CD4+ CD8- thymocytes was high while using Con A stimulus. And Con A stimulation could result in secretion of IL-4, IL-10, IL-6 and a little amount of IFNγ. IL-2 was barely detectable. This is distinct from typical Th0 type cytokines. The cells of this subset were NK1.1 negative, but strongly expressed GATA-3 mRNA. The results suggest that the CD4+ subset of 3G11 - 6C10- NK1.1 - phenotype possesses immunocompetent cells with functions characteristic of Th2-like cytokines, which may indicate the cells at transitional status from ThO to Th2, with a propensity to Th2.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 39730410).
文摘The presence of a relatively mature CD4+CD8- (SP)T cell subset in mouse thymus has been demonstrated. Composing of 10% of total CD4SP thymocytes, this subset is defined by the absence of 3G11 and 6C10 expression with a phenotype of CD69 +/- , HSAmed/lo and heterogeneous for Qa - 2 expression. The proliferation capability of TCRαβ+ 3G11-6C10- CD4+ CD8- thymocytes was high while using Con A stimulus. And Con A stimulation could result in secretion of IL-4, IL-10, IL-6 and a little amount of IFNγ. IL-2 was barely detectable. This is distinct from typical Th0 type cytokines. The cells of this subset were NK1.1 negative, but strongly expressed GATA-3 mRNA. The results suggest that the CD4+ subset of 3G11 - 6C10- NK1.1 - phenotype possesses immunocompetent cells with functions characteristic of Th2-like cytokines, which may indicate the cells at transitional status from ThO to Th2, with a propensity to Th2.
