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Hydrochloric acid‐mediated synthesis of ZnFe_(2)O_(4) small particle decorated one‐dimensional Perylene Diimide S‐scheme heterojunction with excellent photocatalytic ability 被引量:1
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作者 Yangrui Xu Xiaodie Zhu +7 位作者 Huan Yan Panpan Wang Minshan Song Changchang Ma Ziran Chen Jinyu Chu Xinlin Liu Ziyang Lu 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 2022年第4期1111-1122,共12页
The recyclable and stable ZnFe_(2)O_(4) small particle decorated one‐dimensional perylene diimide(PDI)S‐scheme heterojunction(1D PDI/ZnFe_(2)O_(4))is prepared by the hydrochloric acid‐mediated(HCl‐mediated)strateg... The recyclable and stable ZnFe_(2)O_(4) small particle decorated one‐dimensional perylene diimide(PDI)S‐scheme heterojunction(1D PDI/ZnFe_(2)O_(4))is prepared by the hydrochloric acid‐mediated(HCl‐mediated)strategy,interestingly,the morphology of the 1D PDI/ZnFe_(2)O_(4) can also be effectively regulated by HCl‐mediated process,the existence of HCl can regulate PDI into a uniform rod structure,while the co‐existence of HCl and PDI can limit ZnFe_(2)O_(4) to become the uniform small particles.More importantly,based on the 1D rod structure of PDI and the small size effect of ZnFe_(2)O_(4),carriers can migrate to the surface more easily,which can improve the photocatalytic activity.Meanwhile,due to the appropriate energy level structure,the S‐scheme heterojunction structure is formed between PDI and ZnFe_(2)O_(4),which eliminates meaningless photo‐generated charge carriers through recombination and introduces strong redox to further enhance the photodegradation effect,thereby,1D PDI/ZnFe_(2)O_(4) exhibits excellent photocatalytic ability,under the visible light irradiation,the degradation rate of tetracycline(TC)with 1D PDI/ZnFe_(2)O_(4)(66.67%)is 9.18 times that with PDI(7.26%)and 9.73 times that with ZnFe_(2)O_(4)(6.85%).This work proposes new ideas for the assembly of magnetic organic‐inorganic S‐scheme heterojunction photocatalysts. 展开更多
关键词 Hydrochloric acid‐mediated strategy Morphology regulation Perylene diimide/ZnFe_(2)O_(4) sscheme heterojunction Photocatalytic ability
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The Construct of the Story Continuation Writing Task: Insights From the China’s Standards of English Language Ability 被引量:1
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作者 Wei YE Wei REN Shan ZHENG 《Chinese Journal of Applied Linguistics》 2021年第3期382-398,432,共18页
Although the story continuation writing task(SCWT) has attracted much research attention, few studies have investigated the construct of the task, particularly with respect to which language competence it focuses on. ... Although the story continuation writing task(SCWT) has attracted much research attention, few studies have investigated the construct of the task, particularly with respect to which language competence it focuses on. In addition, although some studies have demonstrated that China’s Standards of English Language Ability(CSE) provides comprehensive insights into the development of Chinese learners’ English language proficiency, little research has investigated the relationship between CSE and SCWT. This study drew upon the CSE to develop an SCWT language use inventory to address the above research gaps. A total of 358 high school students were asked to complete the questionnaire, of which 277 valid responses were analyzed. Exploratory factor analysis(EFA) on the CSE-based questionnaire revealed the presence of six factors, namely language correctness, language variety, task planning, connecting-and-generating, selecting-andmining, and organizing. The strategy of connecting-and-generating was the only significant predictor of the learners’ SCWT scores. The paper also discusses the findings relating to the SCWT rating and other integrated writing tasks. 展开更多
关键词 story continuation writing task(sCWT) China’s standards of English Language ability(CsE) CONsTRUCT integrated writing
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Observation of nutrition supporting treatment's improvement in daily living ability of patients with long-term hemodialysis 被引量:1
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作者 左巍 《中国组织工程研究与临床康复》 CAS CSCD 2001年第19期154-155,共2页
关键词 Observation of nutrition supporting treatment’s improvement in daily living ability of patients with long-term hemodialysis
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Synthesis of A Novel Bridged Bis(β-cyclodextrin)s Possessing Phenan- throline Tether and Its Molecular Binding Ability with Dyes
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作者 Li Peng +1 位作者 LIANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2003年第1期9-12,共4页
A novel b-cyclodextrin dimer bearing 2, 9-diformyl-1, 10-phenanthroline tether 4 has been synthesized and its inclusion complexation behavior with two triangular model substrates (RhB and BG) has been investigated thr... A novel b-cyclodextrin dimer bearing 2, 9-diformyl-1, 10-phenanthroline tether 4 has been synthesized and its inclusion complexation behavior with two triangular model substrates (RhB and BG) has been investigated through fluorescence and ultraviolet spectrometry. The result obtained indicated that novel bridged bis(b-cyclodextrin)s could significantly enhance the original molecular binding ability of native b-cyclodextrin by cooperative binding of two hydrophobic cavities. 展开更多
关键词 Bis(β-cyclodextrin)s inclusion complexation dye binding ability.
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Storage time affects the level and diagnostic efficacy of plasma biomarkers for neurodegenerative diseases
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作者 Lifang Zhao Mingkai Zhang +4 位作者 Qimeng Li Xuemin Wang Jie Lu Ying Han Yanning Cai 《Neural Regeneration Research》 SCIE CAS 2025年第8期2373-2381,共9页
Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative diseases.However,little is k... Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative diseases.However,little is known about the long-term stability of these biomarker proteins in plasma samples stored at-80°C.We aimed to explore how storage time would affect the diagnostic accuracy of these biomarkers using a large cohort.Plasma samples from 229 cognitively unimpaired individuals,encompassing healthy controls and those experiencing subjective cognitive decline,as well as 99 patients with cognitive impairment,comprising those with mild cognitive impairment and dementia,were acquired from the Sino Longitudinal Study on Cognitive Decline project.These samples were stored at-80°C for up to 6 years before being used in this study.Our results showed that plasma levels of Aβ42,Aβ40,neurofilament light chain,and glial fibrillary acidic protein were not significantly correlated with sample storage time.However,the level of total tau showed a negative correlation with sample storage time.Notably,in individuals without cognitive impairment,plasma levels of total protein and tau phosphorylated protein threonine 181(p-tau181)also showed a negative correlation with sample storage time.This was not observed in individuals with cognitive impairment.Consequently,we speculate that the diagnostic accuracy of plasma p-tau181 and the p-tau181 to total tau ratio may be influenced by sample storage time.Therefore,caution is advised when using these plasma biomarkers for the identification of neurodegenerative diseases,such as Alzheimer's disease.Furthermore,in cohort studies,it is important to consider the impact of storage time on the overall results. 展开更多
关键词 Alzheimer’s disease amyloid-β diagnostic ability glial fibrillary acidic protein NEURODEGENERATION neurofilament light chain plasma biomarkers single molecule array storage time tau
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Combining ability and gene action studies for yield and fibre traits in Gossypium arboreum using Griffings numerical and Haymans graphical approach
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作者 SUKRUTHA Bhimireddy RAJESWARI Sivakami +3 位作者 PREMALATHA N. BOOPATHI Narayana Manikanda THIRUKUMARAN K. MANIVANNAN A. 《Journal of Cotton Research》 CAS 2023年第3期141-156,共16页
Background For the purpose of utilising hybrid vigour to produce possible hybrids with a suitable level of stability,the knowledge of gene activity and combining ability is a crucial prerequisite before choosing desir... Background For the purpose of utilising hybrid vigour to produce possible hybrids with a suitable level of stability,the knowledge of gene activity and combining ability is a crucial prerequisite before choosing desirable parents.The present study was carried out with six parents crossed in full diallel fashion and generated 30 F1 hybrids.These hybrids were evaluated in two replications in Randomized Block Design at Department of Cotton,TNAU for combining ability and gene action.Diallel analysis was carried out according to Griffing’s method-I(parents + F_(1) + reciprocals) and model-I and Hayman’s graphical approach by using INDOSTAT software.Results Analysis of variance for combining ability indicated that mean square values of GCA,SCA and reciprocals were highly significant for all the traits except for the uniformity index.RG763 and K12 showed highly positively significant GCA effects for most of the yield traits while PA838 and K12 for fibre quality traits,so they were found as best general combiners.PAIG379 × K12 and PDB29 × K12 for yield traits,and PDB29 × PA838,RG763 × PA838,and CNA1007 × RG763 cross combinations for fibre quality traits could be recommended for future breeding programms.Conclusion The results of both Griffing’s and Hayman’s approaches showed that non-additive gene action predominates as SCA variance was bigger than GCA variance,so heterosis breeding is thought to be a more fruitful option for enhancing GCA of many traits. 展开更多
关键词 Gene action Combining ability Diallel analysis Hayman’s approach Griffing’s approach Vr-Wr graph Desi cotton
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Chiral asymmetry of anti-symmetric coordinates studied by the Raman differential bond polarizability of S-phenylethylamine
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作者 沈红霞 吴国祯 王培杰 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第12期191-197,共7页
The Raman optical activity (ROA) study on S-phenylethylamine is presented by the intensity analyses via bond polarizability and differential bond polarizability. Ample information concerning the physical picture of ... The Raman optical activity (ROA) study on S-phenylethylamine is presented by the intensity analyses via bond polarizability and differential bond polarizability. Ample information concerning the physical picture of this chiral system is obtained, and its ROA mechanism is constructed. Especially, we propose that the asymmetric modes and/or the off-diagonal elements of the electronic polarizability tensor are the potential keys to the exploration of ROA. 展开更多
关键词 Raman optical activity intensity study asymmetric modes differential bond polariz-ability s-phenylethylamine
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China's Western Region Has Poor Ability for Sustainable Development
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《China's Foreign Trade》 2000年第4期10-10,共1页
关键词 China’s Western Region Has Poor ability for sustainable Development
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CeO2改性渗铝层在H2S溶液中的耐腐蚀性能
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作者 杨柳松 文九巴 +2 位作者 张金民 朱利敏 李全安 《特种铸造及有色合金》 CAS CSCD 北大核心 2007年第10期793-795,共3页
采用在碳钢表面复合沉积Ni-CeO2扩散渗铝的方法,得到了CeO2改性的复合渗层,在H2S溶液腐蚀条件下对渗层进行了耐腐蚀性能试验研究,并利用SEM对渗铝层的显微组织进行了分析。结果表明,经CeO2改性渗铝的方法获得了富集CeO2的细晶层,同时,... 采用在碳钢表面复合沉积Ni-CeO2扩散渗铝的方法,得到了CeO2改性的复合渗层,在H2S溶液腐蚀条件下对渗层进行了耐腐蚀性能试验研究,并利用SEM对渗铝层的显微组织进行了分析。结果表明,经CeO2改性渗铝的方法获得了富集CeO2的细晶层,同时,渗层的耐H2S腐蚀性能明显提高,腐蚀质量增量为纯铝渗层的40%。 展开更多
关键词 ceo2 改性渗铝 复合沉积 H2s腐蚀
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教育硕士产教融合“U-G-S&E”育人模式探析
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作者 赵兴碧 章光洁 董红梅 《西南林业大学学报(社会科学)》 2024年第4期104-109,共6页
产教融合是教育与产业的深度合作,我国产教融合的政策经历了从职业教育到普通教育的发展。产教融合符合教育硕士人才培养的逻辑,“U-G-S&E”育人模式以高校为主导,政府提供政策引导和资金支持,中小学和企业协同参与教育硕士的培养... 产教融合是教育与产业的深度合作,我国产教融合的政策经历了从职业教育到普通教育的发展。产教融合符合教育硕士人才培养的逻辑,“U-G-S&E”育人模式以高校为主导,政府提供政策引导和资金支持,中小学和企业协同参与教育硕士的培养。通过实践探索发现,“U-G-S&E”模式可以更好地激发学生提升职业能力的动力,助力学生职业生涯规划和就业,提升以赛赋能的效果,帮助高校校内导师、中小学和企业的校外导师加深对教育链、人才链与产业链、创新链有机衔接的理解,助力孵化本地企业参与遴选国家产教融合型企业,推动本地企业更好的融入国家现代教育体系建设。 展开更多
关键词 教育硕士 产教融合 U-G-s&E 职业能力
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ZTE's View on HR Management——A summary of the Interview with Hou Weigui,CEO of ZTE,by Business Celebrities,CCTV
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作者 Zhong Xing (ZTE Corporation,Shenzhen 518057,China) 《ZTE Communications》 2003年第1期48-49,共2页
Anchorman:Today, being people-oriented has become acommon concern in manybusiness organizations.What does“being peo-ple-oriented”mean? Fora product,it meansbringing maximum satis-faction to the customer.
关键词 ZTE’s View on HR Management A summary of the Interview with Hou Weigui ceo of ZTE by Business Celebrities CCTV HR ceo of on by VIEW with
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东丽推出“CEO+S”织物
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作者 王爱梅 《合成技术及应用》 2003年第4期22-22,共1页
东丽公司将为2004年春/夏季在日本市场推出“CEO+S”聚酯机织物,它有干燥手感,精细柔软感。丝为皮芯结构。
关键词 日本 2004年 东丽公司 ceo%PLUs%s”聚酯机织物
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新型S型异质结光催化剂研究进展
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作者 唐青梅 程清蓉 邹明鑫 《化学与生物工程》 CAS 北大核心 2024年第6期13-20,共8页
环境污染是人类目前所面临的最严重的问题之一。光催化技术是一种能直接利用太阳能降解污染物的新型、绿色、清洁的有效方法。然而,单一半导体对太阳光的吸收能力弱,光生载流子的复合率高;传统复合光催化剂氧化还原能力弱,光催化效率低... 环境污染是人类目前所面临的最严重的问题之一。光催化技术是一种能直接利用太阳能降解污染物的新型、绿色、清洁的有效方法。然而,单一半导体对太阳光的吸收能力弱,光生载流子的复合率高;传统复合光催化剂氧化还原能力弱,光催化效率低;构建S型异质结是提高光催化活性的有效策略。比较分析了几种异质结的电荷转移机制,综述了S型异质结的结构调控策略和应用研究进展,并对S型异质结光催化剂的未来发展前景进行了展望。 展开更多
关键词 光催化技术 s型异质结 氧化还原能力
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基于新任CEO视角下的战略变革动因研究 被引量:25
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作者 刘鑫 薛有志 《管理学报》 CSSCI 北大核心 2013年第12期1747-1759,共13页
基于组织理论和代理理论的分析认为,新任CEO相较前任的特征差异及其继任后可能在短期内会面临被解聘的威胁,是新任CEO主动发起战略变革的动力。同时,考虑到CEO变更时的公司业绩和前任CEO任期作为CEO变更时的公司情境因素对新任CEO发... 基于组织理论和代理理论的分析认为,新任CEO相较前任的特征差异及其继任后可能在短期内会面临被解聘的威胁,是新任CEO主动发起战略变革的动力。同时,考虑到CEO变更时的公司业绩和前任CEO任期作为CEO变更时的公司情境因素对新任CEO发起战略变革的影响。应用多元调节回归模型以及均值比较的方法,以2000~2009年中国上市公司为样本对上述观点进行了检验。结果表明,即使公司业绩优异,CEO变更依然与公司战略变革显著正相关;CEO变更时,公司业绩和前任CEO任期显著地负向调节CEO变更对战略变革的影响。 展开更多
关键词 战略变革动因 ceo变更 公司业绩 前任ceo任期
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CEO才能与公司绩效——基于中国上市公司CEO离职数据研究 被引量:3
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作者 马利军 周威 《运筹与管理》 CSSCI CSCD 北大核心 2012年第5期200-207,共8页
首席执行官(Chief Executive Officer,以下简称总经理或CEO)作为公司的管理者,其能力与公司绩效的关系在理论和实践上都受到广泛关注。本文以CEO离职事件为样本,选择1997~2007年间沪深上市公司数据,从分离CEO才能出发,提出了用股票市... 首席执行官(Chief Executive Officer,以下简称总经理或CEO)作为公司的管理者,其能力与公司绩效的关系在理论和实践上都受到广泛关注。本文以CEO离职事件为样本,选择1997~2007年间沪深上市公司数据,从分离CEO才能出发,提出了用股票市场反应和再任职市场反应代替CEO才能。通过对中国实证数据进行回归分析,得到CEO才能与公司绩效是正相关关系,CEO薪酬的获得不是靠运气,而是靠才能,同时通过数学分析发现中国投资市场存在反应过度现象以及中国经理人市场的不完善现象。 展开更多
关键词 公司治理 ceo才能 公司绩效 事件分析 ceo离职
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电针对阿尔茨海默病模型SD大鼠学习记忆功能及海马神经细胞凋亡的影响 被引量:9
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作者 易显富 彭力 +8 位作者 陈向军 张泽月 肖敏 穆敬平 程建明 刘园园 梁晓翠 李攻波 高翔 《实用临床医药杂志》 CAS 2015年第1期1-6,共6页
目的观察电针对阿尔茨海默病(AD)模型SD大鼠学习记忆、海马神经细胞凋亡、谷氨酸(Glu)含量、N-甲基-D-天门冬氨酸(NMDA)受体、caspase-3蛋白、自噬相关蛋白Beclin-1表达的影响。方法 48只健康雌性SD大鼠随机分为AD模型组、假手术... 目的观察电针对阿尔茨海默病(AD)模型SD大鼠学习记忆、海马神经细胞凋亡、谷氨酸(Glu)含量、N-甲基-D-天门冬氨酸(NMDA)受体、caspase-3蛋白、自噬相关蛋白Beclin-1表达的影响。方法 48只健康雌性SD大鼠随机分为AD模型组、假手术组、正常对照组和电针组,每组12只。采用Aβ25~35建立AD模型,Morris水迷宫观察大鼠学习记忆能力,尼氏染色观察海马神经元数量,细胞术检测各组海马神经元的凋亡,高效液相色谱法检测海马Glu含量,免疫组化法检测海马组织NMDA受体表达,Western blotting检测海马caspase-3和beclin-1蛋白的表达。结果与AD模型组比较,电针组SD大鼠在第Ⅱ象限停留时间所占的百分比较大、跨越平台次数较多、目标象限停留时间较长、平台象限停留路程占总路程百分比较大、平均潜伏期较短,学习记忆能力有明显改善;海马区神经元凋亡率较低,NMDA受体功能增强,Glu含量、caspase-3和Beclin-1蛋白表达减少(P〈0.05)。结论电针治疗可改善AD大鼠学习记忆功能,抑制神经元凋亡,上调NMDA受体功能,减少凋亡相关蛋白的含量。 展开更多
关键词 电针 阿尔茨海默病 海马 学习记忆 细胞凋亡
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3种拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的差异 被引量:26
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作者 李斌 谢淑玲 +3 位作者 彭丽燕 王飞 杨昆 伍文彬 《医学研究生学报》 CAS 北大核心 2014年第7期683-685,共3页
目的 Morris水迷宫实验是一种评价空间学习记忆能力的经典方法,是评价痴呆动物模型复制结果的客观指标。近年来痴呆动物模型已成为研究老年性痴呆的重要手段。文中评价3种常见拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的... 目的 Morris水迷宫实验是一种评价空间学习记忆能力的经典方法,是评价痴呆动物模型复制结果的客观指标。近年来痴呆动物模型已成为研究老年性痴呆的重要手段。文中评价3种常见拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的差异。方法分别使用SD大鼠、昆明种小鼠以及快速老化小鼠制备Aβ脑室注射致痴呆模型、东莨菪碱腹腔注射致记忆障碍模型、快速老化模型。采用Morris水迷宫行为学测试方法,将这3种模型通过与其相应的对照组比较,考察其行为学差异。结果东莨菪碱模型与快速老化模型逃避潜伏期时间短于相应对照组,其中以东莨菪碱模型差异尤为显著[(35.47±3.78)s vs(50.61±3.94)s,P<0.01];快速老化组模型与对照组差异有统计学意义[(17.39±4.71)s vs(33.79±7.81)s,P<0.05];Aβ致痴呆模型与其对照组比较,模型定位航行实验成绩差异无统计学意义(P>0.05)。结论东莨菪碱腹腔注射致记忆障碍模型较其他2种常用模型更为理想。Morris水迷宫行为学测试方法是一种评价拟痴呆动物的理想方法。 展开更多
关键词 拟痴呆模型 Morris水迷宫试验 行为学差异 认知与记忆能力 大鼠 小鼠
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S-3307和PP333对水稻秧苗促蘖效应的比较测定及其与植物内源激素关系的研究 被引量:11
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作者 陈善坤 曾晓春 刘传飞 《江西农业学报》 CAS 1994年第1期10-13,共4页
本文报导S─3307和PP333对水稻秧苗促蘖效应的比较测定及其与植物内源激素关系的研究结果:(一)用40ppmS-3307和400ppmPP333进行浸种处理的水稻秧苗,在三叶一心期分别有65.01%和19.79%... 本文报导S─3307和PP333对水稻秧苗促蘖效应的比较测定及其与植物内源激素关系的研究结果:(一)用40ppmS-3307和400ppmPP333进行浸种处理的水稻秧苗,在三叶一心期分别有65.01%和19.79%的植株出现分蘖,对照秧苗无分蘖出现。S-3307和PP333对水稻均有促蘖效应,而前者的促蘖效应大于后者,(二)经S-3307和PP333处理的秧苗以及对照秧苗,在三叶一心期的苗体内,每克鲜重CTK含量分别为1346.4、842.4、86.4pmol/g;CTK/IAA的比例分别为3.69、2.54、0.29.在同期测定了秧苗休内可溶性糖、淀粉和全糖以及可溶性氮、蛋白质和全氮含量,它们在三种处理苗体内无明显差异。(三)本试验结果表明:S-3307对水稻的促蘖效应大于PP333.S-3307和PP333的促蘖效应与其提高水稻秧苗体内CTK含量和CTK/IAA比例密切相关,其中S-3307提高CTK含量和CTK/IAA比例的作用大于PP333,这一点与S-3307的促蘖效应大于PP333的结果是相吻合的。 展开更多
关键词 s-3307 分蘖能力 水稻 多效唑
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T-S模糊系统的构造及其逼近能力的充分条件 被引量:4
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作者 肖小石 毛志忠 袁平 《控制理论与应用》 EI CAS CSCD 北大核心 2009年第8期903-906,共4页
针对任意多变量非线性函数,至今缺少一种简单有效的构造性方法来建造一个T-S模糊系统对其实现给定精度的模糊逼近.本文在逼近函数g(X)∈C2及g(X)∈/C2两种情况下给出了一种当模糊集满足均匀性、连续性、正规性条件下T-S模糊系统的构造... 针对任意多变量非线性函数,至今缺少一种简单有效的构造性方法来建造一个T-S模糊系统对其实现给定精度的模糊逼近.本文在逼近函数g(X)∈C2及g(X)∈/C2两种情况下给出了一种当模糊集满足均匀性、连续性、正规性条件下T-S模糊系统的构造方法以及该T-S模糊系统逼近能力的充分条件.该构造性方法简单易行且其逼近能力充分条件的保守性低.最后通过仿真研究证实了该方法的正确性与有效性. 展开更多
关键词 T-s模糊系统 模糊划分 逼近能力 充分条件
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板桥党参对激活GSK-3β诱导的AD模型大鼠认知功能障碍的保护作用及其机制 被引量:12
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作者 罗洪斌 刘翔宇 +8 位作者 牟南樵 陈玮 樊莎莎 谢文执 商楠 杨晨宇 谢枫枫 谌勤 魏征 《中国药理学通报》 CAS CSCD 北大核心 2017年第8期1060-1067,共8页
目的探索板桥党参(BCP)水煎液对激活糖原合成酶激酶-3β(GSK-3β)诱导的阿尔兹海默病(AD)模型大鼠认知功能障碍的保护作用及其可能机制。方法将50只4月龄♂SD大鼠随机分为假手术组、AD模型组、低剂量板桥党参组、中剂量板桥党参组、高... 目的探索板桥党参(BCP)水煎液对激活糖原合成酶激酶-3β(GSK-3β)诱导的阿尔兹海默病(AD)模型大鼠认知功能障碍的保护作用及其可能机制。方法将50只4月龄♂SD大鼠随机分为假手术组、AD模型组、低剂量板桥党参组、中剂量板桥党参组、高剂量板桥党参组。前2组饮用水灌胃14 d,后3组灌服板桥党参水煎液14 d。灌胃同时进行自主行为测试,d 8开始进行水迷宫训练。训练5 d后,选择能在15 s内找到隐藏平台的治疗组和模型组大鼠进行侧脑室注射渥曼青霉素(Wortmannin,PI3K特异性抑制剂)和GF-109203X(GFX,PKC特异性抑制剂)(浓度均为100μmol·L^(-1))各5μL;假手术组注射2%DMSO 10μL。24 h后进行水迷宫测试。观察各组大鼠侧脑室注射前、后空间学习记忆力是否改变。Western blot和免疫组化检测大鼠脑海马组织Tau磷酸化水平及其相关蛋白激酶GSK-3β表达情况及活性变化。尼氏染色观察神经元尼氏小体变化情况。结果自主行为活动检测结果发现板桥党参能明显减少大鼠活动次数;水迷宫检测结果发现板桥党参能明显改善模型大鼠认知功能障碍;Western blot和免疫组化结果显示模型组海马组织GSK-3β活性升高,并导致Tau蛋白多个位点发生过度磷酸化,而用药后GSK-3β活性降低,Tau蛋白多个位点磷酸化水平下调;尼氏染色结果提示模型组海马神经元内尼氏小体数量减少,而治疗后尼氏小体增多,且呈剂量依赖性。结论板桥党参可有效改善GSK-3β活性升高所诱导的大鼠认知功能障碍,其可能机制与下调GSK-3β活性,进而抑制Tau蛋白过度磷酸化、促进神经元发育有关。 展开更多
关键词 阿尔兹海默病 板桥党参 GsK-3Β TAU磷酸化 学习记忆能力 水迷宫
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