Objective: To evaluate the effects of cryopreservation of APBSCs in ?80°C un-controlled freezer and liquid nitrogen, and to search for the efficient combined cryoprotectant and the highest cell concentration for ...Objective: To evaluate the effects of cryopreservation of APBSCs in ?80°C un-controlled freezer and liquid nitrogen, and to search for the efficient combined cryoprotectant and the highest cell concentration for cryopreservating peripheral blood stem cell (PBSC). Methods: To compare the effect of three combined cryoprotectants, evaluation of in vitro proliferative capacity by colony-forming unit-Granulocyte-macrophage (CFU-GM) and burst-forming Unit-erythroid (BFU-E) assay, immunophenotyping for CD34+/CD38? cells by FCM, and viability assessment by trypan blue exclusion were performed. Results: The cryoprotectant 10%DMSO+HES+auto-Plasma resulted in the highest recovery rates. CFU-GM and BFU-E were (78.7±9.8)%, (69.8±14.1)%, respectively. The recovery rates of CFU-GM and BFU-E in A/C groups were (68.3±6.2)%/ (65.8±7.2)% and (63.4±9.7)%/ (60.4±10.5)%, respectively. The recovery rates of CD34+/CD38? cells and cell viability with the three combined reagents were 90% and 80%, respectively. Conclusion: 5%DMSO+ HES+auto-PLASMA is an ideal combined cryoprotectant for cryopreserving PBSC. The cell concentrations may be cryopreserved at 4×108 ml?1.展开更多
Development of marrow colony forming unit erythrocytic (CFU-E), colony forming unit granulocytic and macrophagic (CFU-GM) and colony forming unit fibroblastic (CPU桭) of chicken anemia virus (CAV) infection were exami...Development of marrow colony forming unit erythrocytic (CFU-E), colony forming unit granulocytic and macrophagic (CFU-GM) and colony forming unit fibroblastic (CPU桭) of chicken anemia virus (CAV) infection were examined by using In vitro culture techniques of hematopoietic progenitor cells. Chicks were inoculated intraperitoneally and intramuscularly with CAV at one day of age. The results showed that CAV inhibited proliferation of CFU-E and CFU-GM of chick bone marrow. In vitro culture, growth of CFU-E was inhibited from day 14 to 35 postinoculation (PI), and growth of CFU-GM was greatly inhibited from 7 to 21 days PI. Growth of CFU-E and CFU-GM recovered at day 42 PI. No effect was found on growth of CFU-F of bone marrow in chicks infected CAV.展开更多
基金This work was supported by a grant from the National"95"Key Project of China(No. 96-906-01-12).
文摘Objective: To evaluate the effects of cryopreservation of APBSCs in ?80°C un-controlled freezer and liquid nitrogen, and to search for the efficient combined cryoprotectant and the highest cell concentration for cryopreservating peripheral blood stem cell (PBSC). Methods: To compare the effect of three combined cryoprotectants, evaluation of in vitro proliferative capacity by colony-forming unit-Granulocyte-macrophage (CFU-GM) and burst-forming Unit-erythroid (BFU-E) assay, immunophenotyping for CD34+/CD38? cells by FCM, and viability assessment by trypan blue exclusion were performed. Results: The cryoprotectant 10%DMSO+HES+auto-Plasma resulted in the highest recovery rates. CFU-GM and BFU-E were (78.7±9.8)%, (69.8±14.1)%, respectively. The recovery rates of CFU-GM and BFU-E in A/C groups were (68.3±6.2)%/ (65.8±7.2)% and (63.4±9.7)%/ (60.4±10.5)%, respectively. The recovery rates of CD34+/CD38? cells and cell viability with the three combined reagents were 90% and 80%, respectively. Conclusion: 5%DMSO+ HES+auto-PLASMA is an ideal combined cryoprotectant for cryopreserving PBSC. The cell concentrations may be cryopreserved at 4×108 ml?1.
文摘Development of marrow colony forming unit erythrocytic (CFU-E), colony forming unit granulocytic and macrophagic (CFU-GM) and colony forming unit fibroblastic (CPU桭) of chicken anemia virus (CAV) infection were examined by using In vitro culture techniques of hematopoietic progenitor cells. Chicks were inoculated intraperitoneally and intramuscularly with CAV at one day of age. The results showed that CAV inhibited proliferation of CFU-E and CFU-GM of chick bone marrow. In vitro culture, growth of CFU-E was inhibited from day 14 to 35 postinoculation (PI), and growth of CFU-GM was greatly inhibited from 7 to 21 days PI. Growth of CFU-E and CFU-GM recovered at day 42 PI. No effect was found on growth of CFU-F of bone marrow in chicks infected CAV.
