The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells...The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells (MMSC) and fibroblast colony forming units (CFU-F). The aim of this study was to compare the main characteristics of MMSC and CFU-F derived from the bone marrow of 24 healthy donors. Growth and differentiation parameters, as well as relative expression levels of different genes were analyzed in MMSC and CFU-F. MMSC were cultivated for 5 passages. CFU-F concentration was determined for each bone marrow sample. The data obtained demonstrated the heterogeneity and hierarchical organization of both studied populations of stromal precursor cells-MMSC and CFU-F. These two types of stromal precursor cells turned to be different in most parameters studied. Altogether MMSC seemed to be more immature cells than CFU-F and took up the higher position in hierarchical tree of mesenchymal stem cells. The rate of differentiation and proliferative potential decreased with the donor’s age in both populations MMSC and CFU-F.展开更多
Objective：This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells（MSC）.Methods：We determined...Objective：This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells（MSC）.Methods：We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from：（1） donors of different ages;（2） healthy donors and patients with leukemia;（3） bone marrow aspirated at different time points during marrow harvesting;（4） bone marrow harvested by different needles.Results：During the process of harvesting,the number of MSC significantly decreased with increase number of aspiration,from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated,and 50/ml after 200 ml bone marrow aspirated.The number of MSC retrieved from leukemia patients（99/ml bone marrow） was significantly lower than that of healthy donors（708/ml bone marrow）.However,there was no significant difference in growth rate.There was no significant age-related difference of MSC yielded from donors 55 years.And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.Conclusion：The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure.Bone marrow from donors 55 years was equally good as MSC sources.The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.展开更多
Development of marrow colony forming unit erythrocytic (CFU-E), colony forming unit granulocytic and macrophagic (CFU-GM) and colony forming unit fibroblastic (CPU桭) of chicken anemia virus (CAV) infection were exami...Development of marrow colony forming unit erythrocytic (CFU-E), colony forming unit granulocytic and macrophagic (CFU-GM) and colony forming unit fibroblastic (CPU桭) of chicken anemia virus (CAV) infection were examined by using In vitro culture techniques of hematopoietic progenitor cells. Chicks were inoculated intraperitoneally and intramuscularly with CAV at one day of age. The results showed that CAV inhibited proliferation of CFU-E and CFU-GM of chick bone marrow. In vitro culture, growth of CFU-E was inhibited from day 14 to 35 postinoculation (PI), and growth of CFU-GM was greatly inhibited from 7 to 21 days PI. Growth of CFU-E and CFU-GM recovered at day 42 PI. No effect was found on growth of CFU-F of bone marrow in chicks infected CAV.展开更多
文摘The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells (MMSC) and fibroblast colony forming units (CFU-F). The aim of this study was to compare the main characteristics of MMSC and CFU-F derived from the bone marrow of 24 healthy donors. Growth and differentiation parameters, as well as relative expression levels of different genes were analyzed in MMSC and CFU-F. MMSC were cultivated for 5 passages. CFU-F concentration was determined for each bone marrow sample. The data obtained demonstrated the heterogeneity and hierarchical organization of both studied populations of stromal precursor cells-MMSC and CFU-F. These two types of stromal precursor cells turned to be different in most parameters studied. Altogether MMSC seemed to be more immature cells than CFU-F and took up the higher position in hierarchical tree of mesenchymal stem cells. The rate of differentiation and proliferative potential decreased with the donor’s age in both populations MMSC and CFU-F.
文摘Objective：This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells（MSC）.Methods：We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from：（1） donors of different ages;（2） healthy donors and patients with leukemia;（3） bone marrow aspirated at different time points during marrow harvesting;（4） bone marrow harvested by different needles.Results：During the process of harvesting,the number of MSC significantly decreased with increase number of aspiration,from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated,and 50/ml after 200 ml bone marrow aspirated.The number of MSC retrieved from leukemia patients（99/ml bone marrow） was significantly lower than that of healthy donors（708/ml bone marrow）.However,there was no significant difference in growth rate.There was no significant age-related difference of MSC yielded from donors 55 years.And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.Conclusion：The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure.Bone marrow from donors 55 years was equally good as MSC sources.The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.
文摘Development of marrow colony forming unit erythrocytic (CFU-E), colony forming unit granulocytic and macrophagic (CFU-GM) and colony forming unit fibroblastic (CPU桭) of chicken anemia virus (CAV) infection were examined by using In vitro culture techniques of hematopoietic progenitor cells. Chicks were inoculated intraperitoneally and intramuscularly with CAV at one day of age. The results showed that CAV inhibited proliferation of CFU-E and CFU-GM of chick bone marrow. In vitro culture, growth of CFU-E was inhibited from day 14 to 35 postinoculation (PI), and growth of CFU-GM was greatly inhibited from 7 to 21 days PI. Growth of CFU-E and CFU-GM recovered at day 42 PI. No effect was found on growth of CFU-F of bone marrow in chicks infected CAV.
