HPLC法同时测定三七总皂苷中人参皂苷Rg_1与Rb_1的含量

目的 建立 HPL C同时测定三七总皂苷中人参皂苷 Rg1 与 Rb1 的方法。方法 采用 HPL C法 ,以茶碱为内标 ,氨基键合相为固定相 ,流动相为乙腈 -水 (80∶ 2 0 ) ,检测波长 2 0 3nm。结果 三七总皂苷中人参皂苷 Rg1 和 Rb1与其他成分分离良好 ,保留时间分别约为 5 .7和 2 1.5 min。人参皂苷 Rg1 在 80～ 2 80μg/ m L (r=0 .9995 ) ,Rb1 在80～ 2 4 0 μg/ m L(r=0 .9993)线性关系良好 ,Rg1 和 Rb1 加样回收率分别为 97.1%和 98.4 %,RSD分别为 2 .4 4 %和 2 .35 %(n=9)。结论 本法操作简便 ,准确 ,重现性好 ,可用于人参及三七的质量控制。

人工虫草对链脲佐菌素所致小鼠1型糖尿病的降糖作用及机制

目的:探讨人工虫草(蝙蝠蛾拟青霉人工发酵菌丝培养物,PHC)对小鼠1型糖尿病的降糖作用及机制。方法:采用少量多次腹腔注射STZ的方法建立1型糖尿病模型,观察药物对胰腺组织、血糖、肝脏SOD、LPO含量、血清IL-2和IL-10分泌以及小鼠脾T淋巴细胞增殖的影响。结果:低剂量PHC能明显降低糖尿病高血糖,升高血清SOD活力,降低LPO含量,且明显降低IL-2水平,抑制脾T淋巴细胞增殖反应。结论:低剂量PHC对STZ所致小鼠1型糖尿病有明显降糖作用,降糖机制与抑制T细胞应答或细胞因子对胰岛β细胞的破坏,以及清除自由基有关。

基于GF-1影像的珍宝岛自然保护区土地覆盖变化分析

作为我国北方地区最具原始性和代表性的湿地,珍宝岛自然保护区于2008年被批准为国家级自然保护区。为研究珍宝岛自然保护区内土地覆盖和覆盖类型的变化情况,采用国产GF-1卫星数据,将遥感技术方法与野外调查法相结合,得到2014年和2016年的珍宝岛土地覆盖分类,并统计了各类地物的面积。通过对比两期数据,分析得到了土地覆盖类型的变化情况以及土地覆盖类型面积的转移情况。

SIRT1基因沉默诱导肝癌细胞老化及其机制

目的探讨慢病毒介导的沉默信息调节因子1(silent information regulator1,SIRT1)基因沉默对肝癌细胞老化的影响及机制。方法通过慢病毒介导的shRNA干扰技术靶向沉默SIRT1的表达,并通过Real-time PCR和Westernblot验证SIRT1基因的沉默效果。BrdU标记实验检测细胞增殖情况,流式细胞仪检测细胞周期变化;β-半乳糖苷酶染色观察肝癌细胞有无老化;Western blot检测衰老相关基因p53和p21蛋白的变化。结果慢病毒介导的shRNA能显著抑制细胞SIRT1的mRNA及蛋白水平。SIRT1沉默能抑制肝癌细胞的增殖,抑制率达70%～80%;同时,SIRT1沉默能显著诱导G1期细胞周期阻滞:感染shCont的细胞处于G1期比例为(44.29±0.36)%,而感染shSIRT1-1的细胞处于G1期细胞为(69.20±1.24)%,感染shSIRT1-2的细胞处于G1期比例为(65.86±1.75)%。SIRT1沉默后肝癌细胞中衰老相关的β-半乳糖苷酶染色显著增高,阳性细胞占40%～50%(P<0.05),并伴随衰老相关基因p53和p21蛋白表达增加。结论 SIRT1基因沉默可能通过p53/p21途径促进细胞衰老。

二陈汤加味对慢性阻塞性肺疾病大鼠肺组织沉默信息调节因子1、内皮素-1表达的影响

目的探索二陈汤加味对慢性阻塞性肺疾病(COPD)大鼠肺组织沉默信息调节因子(Sirt)1、内皮素(ET)-1表达的影响,分析其药效机制。方法将50只SD大鼠随机分正常组、模型组、二陈汤加味低、中、高剂量组〔10,20,40 g/(kg·d)〕,运用气管内滴注脂多糖(LPS)及烟熏法制备COPD大鼠模型,造模完成后灌胃生理盐水、二陈汤加味方治疗,使用潮气量(TV)、呼气流量峰(PEF)等来测定肺功能变化,免疫组织化学法(IHC)及实时荧光定量聚合酶链式反应(qPCR)检测其肺组织内Sirt1、ET-1基因及蛋白表达。结果与正常组比较,模型组TV、PEF含量均显著减小(P<0.01),肺组织中Sirt1蛋白及基因表达显著减弱(P<0.01),肺组织中ET-1蛋白及基因表达显著增强(P<0.01);与模型组相比,各治疗组PEF、TV均显著增加(P<0.05),各治疗组肺组织Sirt1蛋白及mRNA表达均显著升高(P<0.01)、ET-1蛋白及基因表达均显著降低(P<0.01);其中,二陈汤加味中剂量组肺功能PEF、VT显著升高(P<0.05),Sirt1 mRNA及蛋白表达明显升高(P<0.01)、ET-1蛋白及基因表达显著降低(P<0.001)。结论二陈汤加味能够显著对COPD大鼠肺功能和气流受限起到保护作用,通过升高肺组织Sirt1 mRNA和蛋白表达,减弱ET-1 mRNA和蛋白表达,对COPD大鼠炎症反应起到抑制作用,阻止肺组织血管重构。

STUDIES ON THE SYNTHESIS AND BIOLOGICAL ACTIVITY OF 1-ARYLOXYACETYL-4-AROYLTHIOSEMICARBAZIDE DERIVATIVES

A series of new 1-aryloxyacetyl-4-aroylthiosemicarbazides were synthesized by means of solid-liguid phase transfer catalysis. The promoting effects of these new compounds on wheat growth were observed.

南方大口鲶SME-1细胞系的细胞周期

目的ＳＭＥ－１细胞系来源于南方大口鲶（ＳｉｌｕｒｕｓｍｅｒｉｄｉｏｎａｌｉｓＣｈｅｎ）的晚期胚胎，通过对其细胞周期的分析，可为该细胞系提供细胞动力学特征，同时也可为胚胎发育提供理论基础。方法应用流式细胞光度术分析ＳＭＥ－１细胞系的细胞周期分布特点。结果在不同代龄的细胞群体中，７０．１％～８５．６％的细胞滞留于Ｇ１相，１２．２％～２５．３％的细胞进行活跃的ＤＮＡ复制（Ｓ相），处于Ｍ相的细胞有１．６％～４．６％，而且细胞随着传代代龄的增加，Ｇ２／Ｍ相细胞的比例加大。

Cyclopropanation of 1, 1-Dichloro-4-methyl-1,3-pentadiene with Diazoacetate

Several cyclopropanation catalysts for the synthesis of 3(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylates have been studied in this paper. Owing to electron deficiency of 1,1-dichloro-4-methyl-1,3-pentadiene, yield of this reaction is rather law compared with that of 2,5-dimethyl-2,4-pentadiene, Of all catalysts concerned. yield ranged from 25.1% to 47.2%.

MECHANISM OF ELECTROCHEMILUMINESCENCE OF THE NEW REAGENT 6-(2-HYDROXY-DIETHYLAMINOPHENYLAZO)-2, 3-DIHYDRO-1, 4-PHTHALAZINE-1, 4-DIONE CATALYZED BY SILVER (Ⅰ)

The electrochemi luminescence <ecl> of a new reagent 6-<2-hydroxy-4-diethylaminophenylazo>-2, 3-dihydro-1, 4-phthatazine-1, 4-dione <HDEA> in basic aqueous solution was studied. Trace amount of silver showed significant effect on the efficiency of light emission of HDEA during a positive trigonometrical wave pulse was exerted on the electrodes In the present paper, the ecl spectra of HDEA have been measured. λ_(max) is 410 nm. The reaction of HDEA chemiluminescence and the circular voltammetry, ultraviolet-visible spectrometry for the system have been investigated. The possible mechanism of the ecl of HDEA-KCL-KOH-Ag(I) system has been proposed.

Comparative Assay of Hepatitis B and C Virus Infection Markers by Different Assay Kits^1

In order to compare sensitivity of EIA and RIA assay kits for hepatitis B and C virus (HBV and HCV, respectively) infection markers, 100 serum samples in total were collected form 50 adult women each in urban and rural areas in northeast China. The number of positive cases to the three infection markers on HBV (i.e., HBsAg +, anti HBs +, and anti HBc +) and the one on HCV (anti HCV +) were examined in two laboratories, i.e., in Laboratory A with EIA kits produced in China and in Laboratory B with RIA kits. HCV infection positivity (anti HCV +) was examined by EIA kits in both laboratories, but from different sources in and outside of China, respectively. The assay in Laboratory A gave 2 HBsAg + cases out of the 100 cases examined, whereas there were 9 positive cases in Laboratory B. In contrast, 19 cases were positive to anti HCV when examined in Laboratory A, and there were 3 cases in Laboratory B. Thus, the kits used in Laboratory A gave fewer HBsAg + and more anti HCV + cases than the kits used in Laboratory B. The prevalence of anti HBs + or anti HBc + and cases did not differ when assayed in the two laboratories with EIA and RIA kits, respectively. The agreement of positive and negative findings between the two sets of testing were 93%, 93%, 93%, 86% and 82% for HBsAg, anti HBs, anti HBc, HBV (i.e., either positive to anyone of the three markers or negative to all three markers), and anti HCV, respectively. The implication of the observation on epidemiology on HBV and HCV infection prevalence was discussed.

USE OF CATION EXCHANGE RESIN IN SYNTHESIS OF N-SUBSTITUTED-1-AMINOALKANEPHOSPHONATE AND-PHOSPHINIC ACIDS

Strongly acidic cation exchange resin(1x1,H form)has been successfully used as a catalyst in synthesis of diphenyl N-benzyloxycarbonyl-1-aminobenzylphosphonate, N-benzyloxycarbonyl-1-aminobenzylphenylphosphinic acid and N-p-tolylsulfonyl-1-aminobenzyl phenylphosphinic acid in high yields.