本研究旨在克隆小尾寒羊钙粘和蛋白1(Calcium and integrin binding protein 1,CIB1)基因cDNA全长,并在原核载体中表达。参照已发表的CIB1基因的核苷酸序列,设计了1对特异性引物,采用RT-PCR法,从小尾寒羊睾丸组织中扩增获得CIB1基因全长...本研究旨在克隆小尾寒羊钙粘和蛋白1(Calcium and integrin binding protein 1,CIB1)基因cDNA全长,并在原核载体中表达。参照已发表的CIB1基因的核苷酸序列,设计了1对特异性引物,采用RT-PCR法,从小尾寒羊睾丸组织中扩增获得CIB1基因全长cDNA。将其克隆到pMD19-T载体,并进行测序分析。将该基因编码区重组于融合表达质粒pET32a中,构建了重组原核表达载体(pET32a-CIB1)。将其转化到BL21(DE3)pLysS宿主菌中,用IPTG进行诱导表达。结果表明,克隆的CIB1基因cDNA与GenBank上登录的牛、猪、猕猴、人、小鼠、大鼠、黑猩猩等动物该基因序列的同源性达90%以上,编码氨基酸的同源性在93%以上,并且该序列包含有完整的开放阅读框,大小为576 bp。实现了高效特异性融合表达,表达产物的分子质量约为38 ku。本研究结果为进一步研究CIB1蛋白功能打下良好的基础。展开更多
This study＇s purpose is to evaluate and analyse the indoor daylight quality in Pediatrics Ward in JUH （Jordan University Hospital）. It conducts an investigative analysis associated with an evaluative approach for t...This study＇s purpose is to evaluate and analyse the indoor daylight quality in Pediatrics Ward in JUH （Jordan University Hospital）. It conducts an investigative analysis associated with an evaluative approach for the daylight situation in patient rooms in the children section. A multi-method approach used including on-site measurements, and building model to develop a framework for lighting design in Paediatrics Ward, in order to determine whether the current quality meets the recommended values for patient rooms by CIBSE or not. The study considered the following variables： the differences in daylight environments （illuminance, luminance level, daylight factor）, and the physical environment properties of patient rooms in the hospital. The study found that the indoor daylight performance in terms of illuminance, luminance level, and daylight factor in east patient rooms are higher than the recommended values by CIBSE in the area nearest to glass window at the morning and less than the recommended values in the depth of the room at afternoon. Therefore, solar reflective technologies and shading system must be provided for enhanced day lighting control, avoid excessive glare and to guarantee a good level of visual comfort for patients and staff while reducing artificial lighting demand.展开更多
目的探讨云南省部分地区特教学校聋生携带钙整合素结合蛋白2(calcium-and integrin-binding protein 2,CIB2)基因196C>T,272T>C和297 C>G突变的频率.方法实验组选取337名非综合征型耳聋学生,已明确其全部未携带GJB2(35 del G,1...目的探讨云南省部分地区特教学校聋生携带钙整合素结合蛋白2(calcium-and integrin-binding protein 2,CIB2)基因196C>T,272T>C和297 C>G突变的频率.方法实验组选取337名非综合征型耳聋学生,已明确其全部未携带GJB2(35 del G,176_191 del 16,235del C,299_300 del AT)、GJB3(C538T,G547A)、mt DNA 12S r RNA(A1555G,C1494T)和SLC26A4(IVS7_2A>G,A2168G)致聋基因突变,对照组采用150名健康人,外周静脉采集EDTA抗凝管血液成分,提取基因组DNA,通过PCR扩增含CIB2基因196C>T,272T>C和297 C>G的基因片段,扩增产物直接测序鉴定其基因突变的位点.结果在337名耳聋学生和150名健康人群中均未检测到CIB2基因196C>T,272T>C和297 C>G突变.结论 CIB2基因196C>T,272T>C和297C>G并非是云南省非综合征型耳聋患者携带的基因突变热点,为该地区确定聋病基因筛查谱提供重要依据.展开更多
文摘本研究旨在克隆小尾寒羊钙粘和蛋白1(Calcium and integrin binding protein 1,CIB1)基因cDNA全长,并在原核载体中表达。参照已发表的CIB1基因的核苷酸序列,设计了1对特异性引物,采用RT-PCR法,从小尾寒羊睾丸组织中扩增获得CIB1基因全长cDNA。将其克隆到pMD19-T载体,并进行测序分析。将该基因编码区重组于融合表达质粒pET32a中,构建了重组原核表达载体(pET32a-CIB1)。将其转化到BL21(DE3)pLysS宿主菌中,用IPTG进行诱导表达。结果表明,克隆的CIB1基因cDNA与GenBank上登录的牛、猪、猕猴、人、小鼠、大鼠、黑猩猩等动物该基因序列的同源性达90%以上,编码氨基酸的同源性在93%以上,并且该序列包含有完整的开放阅读框,大小为576 bp。实现了高效特异性融合表达,表达产物的分子质量约为38 ku。本研究结果为进一步研究CIB1蛋白功能打下良好的基础。
文摘This study＇s purpose is to evaluate and analyse the indoor daylight quality in Pediatrics Ward in JUH （Jordan University Hospital）. It conducts an investigative analysis associated with an evaluative approach for the daylight situation in patient rooms in the children section. A multi-method approach used including on-site measurements, and building model to develop a framework for lighting design in Paediatrics Ward, in order to determine whether the current quality meets the recommended values for patient rooms by CIBSE or not. The study considered the following variables： the differences in daylight environments （illuminance, luminance level, daylight factor）, and the physical environment properties of patient rooms in the hospital. The study found that the indoor daylight performance in terms of illuminance, luminance level, and daylight factor in east patient rooms are higher than the recommended values by CIBSE in the area nearest to glass window at the morning and less than the recommended values in the depth of the room at afternoon. Therefore, solar reflective technologies and shading system must be provided for enhanced day lighting control, avoid excessive glare and to guarantee a good level of visual comfort for patients and staff while reducing artificial lighting demand.
文摘目的探讨云南省部分地区特教学校聋生携带钙整合素结合蛋白2(calcium-and integrin-binding protein 2,CIB2)基因196C>T,272T>C和297 C>G突变的频率.方法实验组选取337名非综合征型耳聋学生,已明确其全部未携带GJB2(35 del G,176_191 del 16,235del C,299_300 del AT)、GJB3(C538T,G547A)、mt DNA 12S r RNA(A1555G,C1494T)和SLC26A4(IVS7_2A>G,A2168G)致聋基因突变,对照组采用150名健康人,外周静脉采集EDTA抗凝管血液成分,提取基因组DNA,通过PCR扩增含CIB2基因196C>T,272T>C和297 C>G的基因片段,扩增产物直接测序鉴定其基因突变的位点.结果在337名耳聋学生和150名健康人群中均未检测到CIB2基因196C>T,272T>C和297 C>G突变.结论 CIB2基因196C>T,272T>C和297C>G并非是云南省非综合征型耳聋患者携带的基因突变热点,为该地区确定聋病基因筛查谱提供重要依据.
