目的观察慢性乙型肝炎患者(CHB)CISH、SOCS1和SOCS3 m RNA及CD4+T淋巴细胞亚群相关细胞因子的表达,探讨CISH、SOCS1和SOCS3在慢性乙型肝炎病毒(HBV)感染中的作用。方法选取慢性乙型肝炎患者(CHB)31例,乙肝携带者(As C)18例,正常健康者1...目的观察慢性乙型肝炎患者(CHB)CISH、SOCS1和SOCS3 m RNA及CD4+T淋巴细胞亚群相关细胞因子的表达,探讨CISH、SOCS1和SOCS3在慢性乙型肝炎病毒(HBV)感染中的作用。方法选取慢性乙型肝炎患者(CHB)31例,乙肝携带者(As C)18例,正常健康者18例作为对照,采用实时荧光定量PCR的方法检测外周血单个核细胞(PBMC)中CISH、SOCS1和SOCS3 m RNA的表达,ELISA法检测PBMC上清中IL-2、IL-4、IFN-γ、IL-10、IL-17A和TGF-β细胞因子的表达水平,并分析其相关性。结果CHB组患者CISH m RNA表达高于健康对照组(P<0.01),CHB组SOCS3 m RNA表达显著高于健康对照组(P<0.01),较As C组高(P<0.05),SOCS1 m RNA表达量在各组间比较无明显差异(P>0.05);CHB组IL-4的表达量高于As C组,CHB组IFN-γ/IL-4的比值低于健康对照组,As C组IL-4和TGF-β的表达量均高于健康对照组,差异均有统计学意义(P<0.05),CHB组IL-4、IL-17A和TGF-β的表达量均显著高于健康对照组,差异有显著统计学意义(P<0.01);CHB组患者SOCS1 m RNA表达量与CISH m RNA表达量和SOCS3 m RNA表达量之间均存在显著性关联(P=0.004,P=0.002),SOCS3 m RNA表达量与IFN-γ水平之间存在相关性(P=0.037)。结论 CISH和SOCS3基因可能参与慢性乙型肝炎疾病的发生发展,并与CD4+T淋巴细胞亚群变化之间存在一定的相关性。展开更多
Background Some hepatitis B extracellular antigen （HBeAg）-positive chronic hepatitis B （CHB） patients in their immune active phase can clear the virus spontaneously and enter into an inactive hepatitis B virus （...Background Some hepatitis B extracellular antigen （HBeAg）-positive chronic hepatitis B （CHB） patients in their immune active phase can clear the virus spontaneously and enter into an inactive hepatitis B virus （HBV） carrier state,indicating a benign prognosis.In this study,the association between cytokine-inducibie SRC homology 2 domain protein （C/SH） gene polymorphisms at-292 （rs414171） and the spontaneous clearance of HBV in HBeAg-positive CHB patients in immune the active phase was investigated.Methods Seventy HBeAg-positive CHB patients in the immune active phase were followed up for 76 weeks without antiviral therapy.The alanine transaminase,aspartate transaminase,HBV DNA,HBeAg and hepatitis B extracellular antibody levels were tested regularly.At week 76,27 patients were classified into group A （HBV DNA level below 2 104 IU/ml and the value of HBeAg declined below 10％ of the baseline at week 76）,and 43 patients were classified into group B （HBV DNA level higher than 2×104 IU/ml or the value of HBeAg did not decline substantially at week 76）.CISH （rs414171） polymorphisms were also tested using the iPLEX system.Results The HBV DNA levels at week 12 were significantly greater in group B compared with group A （group A：（6.87±1.40） log10IU/ml; group B：（7.61±1.38） log10IU/ml,P=0.034） and the HBeAg values were greater in group B at week 28 compared with group A （P=0.001）.The differences in HBV DNA and HBeAg values increased between the groups over time.Sixteen patients in group A and 11 in group B were genotype AA.Those with genotype AT or TT included 11 in group A and 31 in group B （AA vs.AT and TT,odds ratio 4.10 （95％ confidence interval：1.462-11.491）,P=0.006）.Conclusion CISH gene polymorphisms at-292 （rs414171） are associated with HBV clearance in HBeAg-positive CHB patients in the immune active phase,and AA is a favorable genotype for this effect.展开更多
文摘目的观察慢性乙型肝炎患者(CHB)CISH、SOCS1和SOCS3 m RNA及CD4+T淋巴细胞亚群相关细胞因子的表达,探讨CISH、SOCS1和SOCS3在慢性乙型肝炎病毒(HBV)感染中的作用。方法选取慢性乙型肝炎患者(CHB)31例,乙肝携带者(As C)18例,正常健康者18例作为对照,采用实时荧光定量PCR的方法检测外周血单个核细胞(PBMC)中CISH、SOCS1和SOCS3 m RNA的表达,ELISA法检测PBMC上清中IL-2、IL-4、IFN-γ、IL-10、IL-17A和TGF-β细胞因子的表达水平,并分析其相关性。结果CHB组患者CISH m RNA表达高于健康对照组(P<0.01),CHB组SOCS3 m RNA表达显著高于健康对照组(P<0.01),较As C组高(P<0.05),SOCS1 m RNA表达量在各组间比较无明显差异(P>0.05);CHB组IL-4的表达量高于As C组,CHB组IFN-γ/IL-4的比值低于健康对照组,As C组IL-4和TGF-β的表达量均高于健康对照组,差异均有统计学意义(P<0.05),CHB组IL-4、IL-17A和TGF-β的表达量均显著高于健康对照组,差异有显著统计学意义(P<0.01);CHB组患者SOCS1 m RNA表达量与CISH m RNA表达量和SOCS3 m RNA表达量之间均存在显著性关联(P=0.004,P=0.002),SOCS3 m RNA表达量与IFN-γ水平之间存在相关性(P=0.037)。结论 CISH和SOCS3基因可能参与慢性乙型肝炎疾病的发生发展,并与CD4+T淋巴细胞亚群变化之间存在一定的相关性。
文摘Background Some hepatitis B extracellular antigen （HBeAg）-positive chronic hepatitis B （CHB） patients in their immune active phase can clear the virus spontaneously and enter into an inactive hepatitis B virus （HBV） carrier state,indicating a benign prognosis.In this study,the association between cytokine-inducibie SRC homology 2 domain protein （C/SH） gene polymorphisms at-292 （rs414171） and the spontaneous clearance of HBV in HBeAg-positive CHB patients in immune the active phase was investigated.Methods Seventy HBeAg-positive CHB patients in the immune active phase were followed up for 76 weeks without antiviral therapy.The alanine transaminase,aspartate transaminase,HBV DNA,HBeAg and hepatitis B extracellular antibody levels were tested regularly.At week 76,27 patients were classified into group A （HBV DNA level below 2 104 IU/ml and the value of HBeAg declined below 10％ of the baseline at week 76）,and 43 patients were classified into group B （HBV DNA level higher than 2×104 IU/ml or the value of HBeAg did not decline substantially at week 76）.CISH （rs414171） polymorphisms were also tested using the iPLEX system.Results The HBV DNA levels at week 12 were significantly greater in group B compared with group A （group A：（6.87±1.40） log10IU/ml; group B：（7.61±1.38） log10IU/ml,P=0.034） and the HBeAg values were greater in group B at week 28 compared with group A （P=0.001）.The differences in HBV DNA and HBeAg values increased between the groups over time.Sixteen patients in group A and 11 in group B were genotype AA.Those with genotype AT or TT included 11 in group A and 31 in group B （AA vs.AT and TT,odds ratio 4.10 （95％ confidence interval：1.462-11.491）,P=0.006）.Conclusion CISH gene polymorphisms at-292 （rs414171） are associated with HBV clearance in HBeAg-positive CHB patients in the immune active phase,and AA is a favorable genotype for this effect.
