AIM: To detect the expression of CK20mRNA in peripheral blood of Pancreatic cancer and evaluate its clinical significance.METHODS: Expression of CK20mRNA in peripheral blood was detected by fluorogenic qualitative rev...AIM: To detect the expression of CK20mRNA in peripheral blood of Pancreatic cancer and evaluate its clinical significance.METHODS: Expression of CK20mRNA in peripheral blood was detected by fluorogenic qualitative reverse transcription-polymerase chain reaction (RT-PCR) in 40cases of pancreatic cancer at the night before operation,in 5 cases of benign pancreatic diseases, in 5 cases of healthy individuals. The relationships were investigated between CK20mRNA expression and the clinicopathological variables, and clinical follow-up outcome in those patients with pancreatic cancer having undergone radical resection.RESULTS: Of the 40 patients with pancreatic cancer, 23(57.5%) cases were positive for CK20mRNA expression.CK20mRNA expression was significantly correlated with lymphatic metastasis (P = 0.008), histopathological grading (P = 0.009), and pathological stage (P = 0.021);there was no significant correlation between CK20mRNA expression and age, gender, tumor diameter, and depth of invasion. The cumulative metastasis rates of patients with CK20mRNA expression were higher than those of patients with no CK20mRNA expression within 6 mo (34.7%vs5.9%, P= 0.043) or 12 mo (73.9% vs35.3%, P= 0.02)after operation. CK20mRNA expression in peripheral blood of pancreatic cancer indicated poorer prognosis. The survival rate of patients with CK20mRNA expression was lower than that of patients with negative CK20mRNA expression (Log-Rank = 13.31, P = 0.0003).CONCLUSION: CK20mRNA is a sensitive and specific molecular marker for the detection of micrometastasis in peripheral blood of patients with pancreatic cancer. The CK20mRNA expression in peripheral blood is correlated with biological characteristic of pancreatic cancer. It can help to predict the prognosis of pancreatic cancer after operation, and to determine which patient will benefit from aggressive adjuvant therapies.展开更多
目的 :分析不同术式对结直肠癌患者CK20mRNA的表达影响及其在结直肠癌治疗中的临床意义。方法 :选取2014年4月～2016年3于本科室240例胃肠道手术患者,采用随机数字表随机分成观察组和对照组,各120例。对照组采用开腹式手术,实验组患者...目的 :分析不同术式对结直肠癌患者CK20mRNA的表达影响及其在结直肠癌治疗中的临床意义。方法 :选取2014年4月～2016年3于本科室240例胃肠道手术患者,采用随机数字表随机分成观察组和对照组,各120例。对照组采用开腹式手术,实验组患者采用腹腔镜手术,并对两组患者手术的基本情况、术后切口的愈合以及CK20mRNA的表达情况进项比较。结果 :观察组患者切口长度(8.28±2.17)cm、手术时间(90.25±10.28)min、出血量(122.18±12.03)mL以及肛门排气时间(5.16±4.63)d均显著优于对照组患者切口长度(13.86±3.69)cm、手术时间(111.36±10.24)min、出血量(188.58±20.53)mL以及肛门排气时间(8.22±5.24)d;观察组患者切口甲级愈合率71.67%、乙级愈合率25.83%、丙级、愈合率2.50%均显著优于对照组患者切合甲级愈合愈合率36.67%、乙级愈合率48.33%、丙级愈合率10.00%,具有显著统计学意义,比较两组患者术前术后CK20mRNA的表达,观察组患者术前外周血中CK20mRNA阳性表达率为80.83%,术后阳性表达率为81.67%;对照组患者术前阳性表达率为79.17%,术后阳性表达率为80.83%,差异无统计学意义。结论 :腹腔镜辅助下行结直肠癌根治术与开腹手术一样,并不增加外周静脉血C K20 m R N A的阳性率和术后肿瘤血循环微转移的危险性。展开更多
文摘AIM: To detect the expression of CK20mRNA in peripheral blood of Pancreatic cancer and evaluate its clinical significance.METHODS: Expression of CK20mRNA in peripheral blood was detected by fluorogenic qualitative reverse transcription-polymerase chain reaction (RT-PCR) in 40cases of pancreatic cancer at the night before operation,in 5 cases of benign pancreatic diseases, in 5 cases of healthy individuals. The relationships were investigated between CK20mRNA expression and the clinicopathological variables, and clinical follow-up outcome in those patients with pancreatic cancer having undergone radical resection.RESULTS: Of the 40 patients with pancreatic cancer, 23(57.5%) cases were positive for CK20mRNA expression.CK20mRNA expression was significantly correlated with lymphatic metastasis (P = 0.008), histopathological grading (P = 0.009), and pathological stage (P = 0.021);there was no significant correlation between CK20mRNA expression and age, gender, tumor diameter, and depth of invasion. The cumulative metastasis rates of patients with CK20mRNA expression were higher than those of patients with no CK20mRNA expression within 6 mo (34.7%vs5.9%, P= 0.043) or 12 mo (73.9% vs35.3%, P= 0.02)after operation. CK20mRNA expression in peripheral blood of pancreatic cancer indicated poorer prognosis. The survival rate of patients with CK20mRNA expression was lower than that of patients with negative CK20mRNA expression (Log-Rank = 13.31, P = 0.0003).CONCLUSION: CK20mRNA is a sensitive and specific molecular marker for the detection of micrometastasis in peripheral blood of patients with pancreatic cancer. The CK20mRNA expression in peripheral blood is correlated with biological characteristic of pancreatic cancer. It can help to predict the prognosis of pancreatic cancer after operation, and to determine which patient will benefit from aggressive adjuvant therapies.
文摘目的 :分析不同术式对结直肠癌患者CK20mRNA的表达影响及其在结直肠癌治疗中的临床意义。方法 :选取2014年4月～2016年3于本科室240例胃肠道手术患者,采用随机数字表随机分成观察组和对照组,各120例。对照组采用开腹式手术,实验组患者采用腹腔镜手术,并对两组患者手术的基本情况、术后切口的愈合以及CK20mRNA的表达情况进项比较。结果 :观察组患者切口长度(8.28±2.17)cm、手术时间(90.25±10.28)min、出血量(122.18±12.03)mL以及肛门排气时间(5.16±4.63)d均显著优于对照组患者切口长度(13.86±3.69)cm、手术时间(111.36±10.24)min、出血量(188.58±20.53)mL以及肛门排气时间(8.22±5.24)d;观察组患者切口甲级愈合率71.67%、乙级愈合率25.83%、丙级、愈合率2.50%均显著优于对照组患者切合甲级愈合愈合率36.67%、乙级愈合率48.33%、丙级愈合率10.00%,具有显著统计学意义,比较两组患者术前术后CK20mRNA的表达,观察组患者术前外周血中CK20mRNA阳性表达率为80.83%,术后阳性表达率为81.67%;对照组患者术前阳性表达率为79.17%,术后阳性表达率为80.83%,差异无统计学意义。结论 :腹腔镜辅助下行结直肠癌根治术与开腹手术一样,并不增加外周静脉血C K20 m R N A的阳性率和术后肿瘤血循环微转移的危险性。
