orf224 is a CMS-related mitochondrial gene discovered in Polima cytoplasm. Shaan 2A CMS line is the parent of the first rapeseed hybrid cultivar Qinyou No. 2 that has been grown in many regions of China. In this work,...orf224 is a CMS-related mitochondrial gene discovered in Polima cytoplasm. Shaan 2A CMS line is the parent of the first rapeseed hybrid cultivar Qinyou No. 2 that has been grown in many regions of China. In this work, genomic DNA of Polima CMS line and Shaan 2A CMS line were used as templates, two primers of specific oligonucleo-tides at 5’ and 3’ ends were used, PCR was performed, the amplification fragments were cloned into pGEM-T Easy vectors and DNA sequences were determined. The CMS-associated gene, orf224-1 present in Shaan 2A CMS line, has a sequence highly homologous to the orf224 of the Polima CMS line, except for one nucleotidc at position +398. There were only one base (AAC→AGC) and one amino acid (Asn→Ser) differences between the two. The homologies of the two sequences in nucleotide and amino acid were 99.9% and 99.6%, respectively. It is concluded that orf224 in Polima CMS line and orf224-1 of Shaan 2A CMS line are the allele at the same locus in mitochondria.展开更多
The biaxial tensile tests were carried out to investigate the deformation behavior and yield loci of dual-phase （DP） steel sheets under biaxial tensile conditions. The true stress-true strain curves of DP steel shee...The biaxial tensile tests were carried out to investigate the deformation behavior and yield loci of dual-phase （DP） steel sheets under biaxial tensile conditions. The true stress-true strain curves of DP steel sheets for different loading ratios were obtained, and the experimental yield loci were determined based on the equivalent plastic work principle. The experimental yield loci were compared to the theoretical yield loci based on Hi1148, Hi1190, Hi1193, Hosford, Barlat89, Yld2000-2d and Gotoh yield criteria. It is found that Yld2000-2d yield criterion can describe the general trends of experimental yield loci of DP steel sheets with comparably higher accuracy. Hosford criterion has the maximum error for DP590, whereas Hi1148 and Barlat89 have the maximum errors for DP780 and DP980.展开更多
基金Supported by Youth Science Foundation of Nanchang Institute of Technology(No.2012KJ023)National College Student’s Innovation and En-trepreneurship Training Program(No.201311319029)~~
基金This work was supported bythe National Key Biotechnology Laboratory for Tropical Crops of China (NKBLTC199802).
文摘orf224 is a CMS-related mitochondrial gene discovered in Polima cytoplasm. Shaan 2A CMS line is the parent of the first rapeseed hybrid cultivar Qinyou No. 2 that has been grown in many regions of China. In this work, genomic DNA of Polima CMS line and Shaan 2A CMS line were used as templates, two primers of specific oligonucleo-tides at 5’ and 3’ ends were used, PCR was performed, the amplification fragments were cloned into pGEM-T Easy vectors and DNA sequences were determined. The CMS-associated gene, orf224-1 present in Shaan 2A CMS line, has a sequence highly homologous to the orf224 of the Polima CMS line, except for one nucleotidc at position +398. There were only one base (AAC→AGC) and one amino acid (Asn→Ser) differences between the two. The homologies of the two sequences in nucleotide and amino acid were 99.9% and 99.6%, respectively. It is concluded that orf224 in Polima CMS line and orf224-1 of Shaan 2A CMS line are the allele at the same locus in mitochondria.
基金This research was funded by National Natural Science Foundation of China （51275026） and State Key Laboratory of Development and Application Technology of Automotive Steels （Bao Steel Y 12ECEQ07Y）.
文摘The biaxial tensile tests were carried out to investigate the deformation behavior and yield loci of dual-phase （DP） steel sheets under biaxial tensile conditions. The true stress-true strain curves of DP steel sheets for different loading ratios were obtained, and the experimental yield loci were determined based on the equivalent plastic work principle. The experimental yield loci were compared to the theoretical yield loci based on Hi1148, Hi1190, Hi1193, Hosford, Barlat89, Yld2000-2d and Gotoh yield criteria. It is found that Yld2000-2d yield criterion can describe the general trends of experimental yield loci of DP steel sheets with comparably higher accuracy. Hosford criterion has the maximum error for DP590, whereas Hi1148 and Barlat89 have the maximum errors for DP780 and DP980.
