Northern blot analysis was conducted with mitochondrial RNA from seedling leaves, floral buds, and developing seeds of NCa CMS, maintainer line and fertile F1 using ten mitochondrial genes as probes. The results revea...Northern blot analysis was conducted with mitochondrial RNA from seedling leaves, floral buds, and developing seeds of NCa CMS, maintainer line and fertile F1 using ten mitochondrial genes as probes. The results revealed that 9 out of the 10 mitochondrial genes, except for atp6, showed no difference in different tissues of the corresponding materials of NCα CMS system and that they might be constitutively expressed genes. Eight genes, such as orf139, orf222, atpl, cox1, cox2, cob, rm5S, and rm26S, showed no difference among the three tissues of all the materials detected. So the expression of these eight genes was not regulated by nuclear genes and was not tissue-specific. The transcripts of atp9 were identical among different tissues, but diverse among different materials, indicating that transcription of atp9 was neither controlled by nuclear gene nor tissue-specific. Gene atp6 displayed similar transcripts with the same size among different tissues of all the materials but differed in abundance among tissues of corresponding materials and its expression might be tissue-specific under regulation of nuclear gene. Moreover, three transcripts of orf222 were detected in the floral buds of NCa cms and fertile F1, but no transcript was detected in floral buds of the maintainer line.The transcription of orf139 was similar to that of orf222 but only two transcripts of 0.8 kb and 0.6 kb were produced. The atp9 probe detected a single transcript of 0.6 kb in NCa cms and in maintainer line and an additional transcript of 1.2 kb in fertile F1. The relationship of expression of orf222, orf139, and atp9 with NCa sterility was discussed.展开更多
The concentration preprocessing and fan-out(CPPF) system is one of the electronic subsystems of the upgraded Compact Muon Solenoid(CMS) Level-1 trigger system. It includes, in hardware, eight specially designed CPPF c...The concentration preprocessing and fan-out(CPPF) system is one of the electronic subsystems of the upgraded Compact Muon Solenoid(CMS) Level-1 trigger system. It includes, in hardware, eight specially designed CPPF cards, one CMS card called AMC13, one commercial Micro-TCA Carrier HUB(MCH) card, and a MicroTCA shelf. Powerful online software is needed for the system, including providing reliable configuration and monitoring for the hardware, and a graphical interface for executing all actions and publishing monitoring messages.Further, to control and monitor the large amount of homogeneous hardware, the SoftWare Automating conTrol of Common Hardware(SWATCH) concept was proposed and developed. The SWATCH provides a generic structure and is flexible for customization. The structure includes a hardware access library based on the IPbus protocol, which assumes a virtual 32-bit address/32-bit data bus and builds a simple hardware access layer. Furthermore, the structure provides a graphical user interface, which is based on modern web technology and is accessible by web page. The CPPF controlling and monitoring online software was also customized from a common SWATCH cell, and provides afinite state machine(FSM) for configuring the entire CPPF hardware, and five monitoring objects for periodically collecting monitoring data from five main functional modules in the CPPF hardware. This paper introduces the details of the CPPF SWATCH cell development.展开更多
In the paper, the problem of storing and replaying of HTTP requests to websites in order to improve their debugging efficiency during development and further support is considered. To solve this problem, the automatic...In the paper, the problem of storing and replaying of HTTP requests to websites in order to improve their debugging efficiency during development and further support is considered. To solve this problem, the automatic HTTP requests logging and replaying subsystem Dagger that provides storage and playback of the requests sent to a website, which is developed by CMS (content management system) Plone means, is offered. The subsystem consists of the three components: Detector, Logger and Player. Detector detects filters user's requests, creates a log and sends it to Logger. Logger is a daemon program that stores logs of user's work with a website. The logs are stored in the files in one of the supported formats: JSON, CSV, PICKLE. Player replays the GET and POST HTTP requests that users sent to a server.展开更多
Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a...Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.展开更多
The CMS Collaboration of 2000 scientists involves 150 institutions from 31 nations spread all over the world.CMS software system integration and release management is performed at CERN.Code management is based on CVS,...The CMS Collaboration of 2000 scientists involves 150 institutions from 31 nations spread all over the world.CMS software system integration and release management is performed at CERN.Code management is based on CVS,with read or write access to the repository via a CVS server,Software configuration,release management tools(SCRAM) are being developed at CERN.Software releases are then distributed to regional centers,where the software is used by a local community for a wide variety of tasks,such as software development detector simulation and reconstruction and physics analysis.Depending on specific application,the system environment and local hardware requirements,different approaches and tools are used for the CMS software installation at different places.This presentation describes concepts and reactial solutions for a variety of ways of software distribution,with an emphasis on the CMS experience at Fermilab,Installation and usage of different models used for the production farm,for code development and for physics analysis are described.展开更多
The CMS regional calorimeter trigger system detects signatures of electrons/photons,taus,jets,and missing and total transverse energy in a deadtinmess pipelined architecture .This system receives 7000 calorimeter treg...The CMS regional calorimeter trigger system detects signatures of electrons/photons,taus,jets,and missing and total transverse energy in a deadtinmess pipelined architecture .This system receives 7000 calorimeter tregger tower energies on 1.2 Gband digital copper cable serial links and processes them in a low-latency pipelined design using custom-built electronics.At the heart of the system is the Receiver Card which uses the new generation of gigabit ethernet receiver chips on a mezzanine card to convert serial data to parallel data before transmission on a 160 MHz backplane for further processing by cards that sum energies and identify electrons and jets.We describe the algorithms and hardware implementation,and summarize the simulation results that show that this system is capable of handling the rate requirements while triggering on physics signals with high efficiency.展开更多
优质的音响器材离不开避震产品,CMS(Critical Mass Systems)带来Olympus-Ultra系列机架助力骏韵音响演示。CMS的老板兼设计师Lavrencik先生在2024中国香港高级音响展上介绍产品:Olympus-Ultra是以Olympus系列为基础的升级,除了机架材质...优质的音响器材离不开避震产品,CMS(Critical Mass Systems)带来Olympus-Ultra系列机架助力骏韵音响演示。CMS的老板兼设计师Lavrencik先生在2024中国香港高级音响展上介绍产品:Olympus-Ultra是以Olympus系列为基础的升级,除了机架材质外,承板、脚钉也都有升级,升级改进的幅度很大,相当于重新设计了。而CMS的其他系列,包括MAXXUM、Platinum、Diamond等也相继迎来了Ultra升级,还有Ultra-Q入门机架系列,全线产品都已更新。Ultra升级后成本提高了不少,但售价变化不大。目标是将机架的存在感降到最低,不影响器材表现,还原器材原本的声音。展开更多
本文利用联合国商品贸易数据库,通过恒定市场份额模型(CMS模型)深入研究中国对RCEP其他成员国纺织品出口的影响因素。并通过分析这些因素,为政府和企业提供有针对性的政策建议和市场战略,以实现纺织品出口的增长和优化。本文发现结构效...本文利用联合国商品贸易数据库,通过恒定市场份额模型(CMS模型)深入研究中国对RCEP其他成员国纺织品出口的影响因素。并通过分析这些因素,为政府和企业提供有针对性的政策建议和市场战略,以实现纺织品出口的增长和优化。本文发现结构效应、竞争力效应、交叉效应都推动了我国对RCEP国家纺织品的出口,其中结构效应的贡献最高,交叉效应次之,竞争力效应贡献较低。经二层分解后发现增长效应发挥了主要作用;同时中国出口的纺织品结构与RCEP市场进口需求相适应;竞争力效应的分解显示中国特定产品的竞争力仍有待提升。This paper uses UN Comtrade to conduct an in-depth study of the factors affecting China’s textile exports to other RCEP member countries through the Constant-Market-Share Model (CMS model). It also provides targeted policy recommendations and market strategies for governments and enterprises to achieve the growth and optimization of textile exports. This paper finds that the structural effect, competitiveness effect, and cross effect all promote China’s textile exports to RCEP countries, with the structural effect making the highest contribution, the cross effect the second highest, and the competitiveness effect making a lower contribution. After two-level decomposition, it is found that the growth effect plays a major role;at the same time, the textile structure of China’s exports is compatible with the import demand of the RCEP market;the decomposition of the competitiveness effect shows that the competitiveness of China’s specific products still needs to be improved.展开更多
基金This work was supported by the National High Technology R&D Project of China (No.2002AA207009) and Wuhan Dawn Project for Youth (No. 20035002016-36).
文摘Northern blot analysis was conducted with mitochondrial RNA from seedling leaves, floral buds, and developing seeds of NCa CMS, maintainer line and fertile F1 using ten mitochondrial genes as probes. The results revealed that 9 out of the 10 mitochondrial genes, except for atp6, showed no difference in different tissues of the corresponding materials of NCα CMS system and that they might be constitutively expressed genes. Eight genes, such as orf139, orf222, atpl, cox1, cox2, cob, rm5S, and rm26S, showed no difference among the three tissues of all the materials detected. So the expression of these eight genes was not regulated by nuclear genes and was not tissue-specific. The transcripts of atp9 were identical among different tissues, but diverse among different materials, indicating that transcription of atp9 was neither controlled by nuclear gene nor tissue-specific. Gene atp6 displayed similar transcripts with the same size among different tissues of all the materials but differed in abundance among tissues of corresponding materials and its expression might be tissue-specific under regulation of nuclear gene. Moreover, three transcripts of orf222 were detected in the floral buds of NCa cms and fertile F1, but no transcript was detected in floral buds of the maintainer line.The transcription of orf139 was similar to that of orf222 but only two transcripts of 0.8 kb and 0.6 kb were produced. The atp9 probe detected a single transcript of 0.6 kb in NCa cms and in maintainer line and an additional transcript of 1.2 kb in fertile F1. The relationship of expression of orf222, orf139, and atp9 with NCa sterility was discussed.
基金supported by the National Natural Science Foundation of China(No.11435013)the National Key Program for S&T Research and Development(No.2016YFA0400104)
文摘The concentration preprocessing and fan-out(CPPF) system is one of the electronic subsystems of the upgraded Compact Muon Solenoid(CMS) Level-1 trigger system. It includes, in hardware, eight specially designed CPPF cards, one CMS card called AMC13, one commercial Micro-TCA Carrier HUB(MCH) card, and a MicroTCA shelf. Powerful online software is needed for the system, including providing reliable configuration and monitoring for the hardware, and a graphical interface for executing all actions and publishing monitoring messages.Further, to control and monitor the large amount of homogeneous hardware, the SoftWare Automating conTrol of Common Hardware(SWATCH) concept was proposed and developed. The SWATCH provides a generic structure and is flexible for customization. The structure includes a hardware access library based on the IPbus protocol, which assumes a virtual 32-bit address/32-bit data bus and builds a simple hardware access layer. Furthermore, the structure provides a graphical user interface, which is based on modern web technology and is accessible by web page. The CPPF controlling and monitoring online software was also customized from a common SWATCH cell, and provides afinite state machine(FSM) for configuring the entire CPPF hardware, and five monitoring objects for periodically collecting monitoring data from five main functional modules in the CPPF hardware. This paper introduces the details of the CPPF SWATCH cell development.
文摘In the paper, the problem of storing and replaying of HTTP requests to websites in order to improve their debugging efficiency during development and further support is considered. To solve this problem, the automatic HTTP requests logging and replaying subsystem Dagger that provides storage and playback of the requests sent to a website, which is developed by CMS (content management system) Plone means, is offered. The subsystem consists of the three components: Detector, Logger and Player. Detector detects filters user's requests, creates a log and sends it to Logger. Logger is a daemon program that stores logs of user's work with a website. The logs are stored in the files in one of the supported formats: JSON, CSV, PICKLE. Player replays the GET and POST HTTP requests that users sent to a server.
基金supported by Heilongjiang Provincial Natural Science Foundation of China(Grant No.YQ2022C012)China Postdoctoral Science Foundation(Grant No.2022MD713728)+1 种基金Heilongjiang Provincial Postdoctoral Fund(Grant No.LBHZ21046)the Open Project of Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,and National Key Research and Development Program of China(Grant No.2023YFD1201501).
文摘Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.
文摘The CMS Collaboration of 2000 scientists involves 150 institutions from 31 nations spread all over the world.CMS software system integration and release management is performed at CERN.Code management is based on CVS,with read or write access to the repository via a CVS server,Software configuration,release management tools(SCRAM) are being developed at CERN.Software releases are then distributed to regional centers,where the software is used by a local community for a wide variety of tasks,such as software development detector simulation and reconstruction and physics analysis.Depending on specific application,the system environment and local hardware requirements,different approaches and tools are used for the CMS software installation at different places.This presentation describes concepts and reactial solutions for a variety of ways of software distribution,with an emphasis on the CMS experience at Fermilab,Installation and usage of different models used for the production farm,for code development and for physics analysis are described.
文摘The CMS regional calorimeter trigger system detects signatures of electrons/photons,taus,jets,and missing and total transverse energy in a deadtinmess pipelined architecture .This system receives 7000 calorimeter tregger tower energies on 1.2 Gband digital copper cable serial links and processes them in a low-latency pipelined design using custom-built electronics.At the heart of the system is the Receiver Card which uses the new generation of gigabit ethernet receiver chips on a mezzanine card to convert serial data to parallel data before transmission on a 160 MHz backplane for further processing by cards that sum energies and identify electrons and jets.We describe the algorithms and hardware implementation,and summarize the simulation results that show that this system is capable of handling the rate requirements while triggering on physics signals with high efficiency.
文摘优质的音响器材离不开避震产品,CMS(Critical Mass Systems)带来Olympus-Ultra系列机架助力骏韵音响演示。CMS的老板兼设计师Lavrencik先生在2024中国香港高级音响展上介绍产品:Olympus-Ultra是以Olympus系列为基础的升级,除了机架材质外,承板、脚钉也都有升级,升级改进的幅度很大,相当于重新设计了。而CMS的其他系列,包括MAXXUM、Platinum、Diamond等也相继迎来了Ultra升级,还有Ultra-Q入门机架系列,全线产品都已更新。Ultra升级后成本提高了不少,但售价变化不大。目标是将机架的存在感降到最低,不影响器材表现,还原器材原本的声音。
文摘本文利用联合国商品贸易数据库,通过恒定市场份额模型(CMS模型)深入研究中国对RCEP其他成员国纺织品出口的影响因素。并通过分析这些因素,为政府和企业提供有针对性的政策建议和市场战略,以实现纺织品出口的增长和优化。本文发现结构效应、竞争力效应、交叉效应都推动了我国对RCEP国家纺织品的出口,其中结构效应的贡献最高,交叉效应次之,竞争力效应贡献较低。经二层分解后发现增长效应发挥了主要作用;同时中国出口的纺织品结构与RCEP市场进口需求相适应;竞争力效应的分解显示中国特定产品的竞争力仍有待提升。This paper uses UN Comtrade to conduct an in-depth study of the factors affecting China’s textile exports to other RCEP member countries through the Constant-Market-Share Model (CMS model). It also provides targeted policy recommendations and market strategies for governments and enterprises to achieve the growth and optimization of textile exports. This paper finds that the structural effect, competitiveness effect, and cross effect all promote China’s textile exports to RCEP countries, with the structural effect making the highest contribution, the cross effect the second highest, and the competitiveness effect making a lower contribution. After two-level decomposition, it is found that the growth effect plays a major role;at the same time, the textile structure of China’s exports is compatible with the import demand of the RCEP market;the decomposition of the competitiveness effect shows that the competitiveness of China’s specific products still needs to be improved.