目的通过观察推拿对坐骨神经慢性压迫性损伤(chronic constriction injury of the sciatic nerve,CCI)模型鼠脊髓背角胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体2B亚...目的通过观察推拿对坐骨神经慢性压迫性损伤(chronic constriction injury of the sciatic nerve,CCI)模型鼠脊髓背角胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体2B亚基(NR2B)表达的影响,探讨推拿治疗神经病理性疼痛的镇痛机制。方法建立CCI模型,将大鼠分为假手术组、模型组、推拿组,每组12只,采用按摩推拿手法模拟仪定性、定量模拟点法、拨法、揉法,对推拿组大鼠右侧的殷门、承山、阳陵泉穴进行干预,通过机械缩足反射阈值和累计疼痛评分评价大鼠机械痛敏反应和疼痛强度变化;通过透射电镜对大鼠脊髓背角突触超微结构进行观察;采用免疫荧光双重标记法观察脊髓背角GFAP、NR2B的共定位表达。结果推拿可以提高CCI模型鼠机械缩足反射阈值、降低累计疼痛评分(P<0.01);同时也能改变大鼠脊髓背角突触的形态结构:与模型组相比,推拿组脊髓背角突触形态结构恢复,膜较完整、线粒体肿胀程度轻、突触后致密区变长。干预20天后,与模型组比较,推拿组大鼠脊髓背角GFAP、NR2B平均荧光强度显著降低(P<0.05)。结论推拿可以改善大鼠的行为学和形态学结果,改变脊髓背角突触可塑性来抑制痛觉敏化;还可以通过抑制星型胶质细胞活化,降低脊髓背角GFAP和NR2B的表达来镇痛。展开更多
A vibro-impact forming machine with double masses is considered. The components of the vibrating system collide with each other. Such models play an important role in the studies of dynamics of mechanical systems with...A vibro-impact forming machine with double masses is considered. The components of the vibrating system collide with each other. Such models play an important role in the studies of dynamics of mechanical systems with impacting components. The Poincaré section associated with the state of the impact-forming system, just immediately after the impact, is chosen, and the period n single-impact motion and its disturbed map are derived analytically. A center manifold theorem technique is applied to reduce the Poincaré map to a two-dimensional map, and the normal form map associated with codimension two bifurcation of 1：2 resonance is obtained, Unfolding of the normal form map is analyzed. Dynamical behavior of the impact-forming system, near the point of codimension two bifurcation, is investigated by using qualitative analyses and numerical simulation. Near the point of codimension two bifurcation there exists not only Neimark-Sacker bifurcation associated with period one single-impact motion, but also Neimark-Sacker bifurcation of period two double-impact motion. Transition of different forms of fixed points of single-impact periodic orbits, near the bifurcation point, is demonstrated, and different routes from periodic impact motions to chaos are also discussed.展开更多
文摘目的通过观察推拿对坐骨神经慢性压迫性损伤(chronic constriction injury of the sciatic nerve,CCI)模型鼠脊髓背角胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体2B亚基(NR2B)表达的影响,探讨推拿治疗神经病理性疼痛的镇痛机制。方法建立CCI模型,将大鼠分为假手术组、模型组、推拿组,每组12只,采用按摩推拿手法模拟仪定性、定量模拟点法、拨法、揉法,对推拿组大鼠右侧的殷门、承山、阳陵泉穴进行干预,通过机械缩足反射阈值和累计疼痛评分评价大鼠机械痛敏反应和疼痛强度变化;通过透射电镜对大鼠脊髓背角突触超微结构进行观察;采用免疫荧光双重标记法观察脊髓背角GFAP、NR2B的共定位表达。结果推拿可以提高CCI模型鼠机械缩足反射阈值、降低累计疼痛评分(P<0.01);同时也能改变大鼠脊髓背角突触的形态结构:与模型组相比,推拿组脊髓背角突触形态结构恢复,膜较完整、线粒体肿胀程度轻、突触后致密区变长。干预20天后,与模型组比较,推拿组大鼠脊髓背角GFAP、NR2B平均荧光强度显著降低(P<0.05)。结论推拿可以改善大鼠的行为学和形态学结果,改变脊髓背角突触可塑性来抑制痛觉敏化;还可以通过抑制星型胶质细胞活化,降低脊髓背角GFAP和NR2B的表达来镇痛。
基金The project supported by the National Natural Science Foundation of China （10572055, 50475109） and the Natural Science Foundation of Gansu Province Government of China （3ZS051-A25-030（key item）） The English text was polished by Keren Wang.
文摘A vibro-impact forming machine with double masses is considered. The components of the vibrating system collide with each other. Such models play an important role in the studies of dynamics of mechanical systems with impacting components. The Poincaré section associated with the state of the impact-forming system, just immediately after the impact, is chosen, and the period n single-impact motion and its disturbed map are derived analytically. A center manifold theorem technique is applied to reduce the Poincaré map to a two-dimensional map, and the normal form map associated with codimension two bifurcation of 1：2 resonance is obtained, Unfolding of the normal form map is analyzed. Dynamical behavior of the impact-forming system, near the point of codimension two bifurcation, is investigated by using qualitative analyses and numerical simulation. Near the point of codimension two bifurcation there exists not only Neimark-Sacker bifurcation associated with period one single-impact motion, but also Neimark-Sacker bifurcation of period two double-impact motion. Transition of different forms of fixed points of single-impact periodic orbits, near the bifurcation point, is demonstrated, and different routes from periodic impact motions to chaos are also discussed.
