Ovalbumin(OVA)is the major allergenic protein that can induce T helper 2(Th2)-allergic reactions,for which current treatment options are inadequate.In this study,we developed a polymerized hypoallergenic OVA product v...Ovalbumin(OVA)is the major allergenic protein that can induce T helper 2(Th2)-allergic reactions,for which current treatment options are inadequate.In this study,we developed a polymerized hypoallergenic OVA product via laccase/caffeic acid(Lac/CA)-catalyzed crosslinking in conjunction with galactomannan(Man).The formation of high molecular weight crosslinked polymers and the Ig G-binding were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting.The study indicated that Lac/CA-catalyzed crosslinking plus Man conjugation substantially altered secondary and tertiary structures of OVA along with the variation in surface hydrophobicity.Gastrointestinal digestion stability assay indicated that crosslinked OVA exhibited less resistance in simulated gastric fluid(SGF)and simulated intestinal fluid(SIF).Mouse model study indicated that Lac-Man/OVA ameliorated eosinophilic airway inflammatory response and efficiently downregulated the expression of Th2-related cytokines(interleukin(IL)-4,IL-5,and IL-13),and upregulated IFN-γand IL-10 expression.Stimulation of bone marrow-derived dendritic cells with Lac-Man/OVA suppressed the expression of phenotypic maturation markers(CD80 and CD86)and MHC class II molecules,and suppressed the expression levels of proinflammatory cytokines.The knowledge obtained in the present study offers an effective way to acquire a hypoallergenic OVA product that can have a therapeutic effect in alleviating OVA-induced allergic asthma.展开更多
The scientific community recognizes the seriousness of rockbursts and the need for effective mitigation measures.The literature reports various successful applications of machine learning(ML)models for rockburst asses...The scientific community recognizes the seriousness of rockbursts and the need for effective mitigation measures.The literature reports various successful applications of machine learning(ML)models for rockburst assessment;however,a significant question remains unanswered:How reliable are these models,and at what confidence level are classifications made?Typically,ML models output single rockburst grade even in the face of intricate and out-of-distribution samples,without any associated confidence value.Given the susceptibility of ML models to errors,it becomes imperative to quantify their uncertainty to prevent consequential failures.To address this issue,we propose a conformal prediction(CP)framework built on traditional ML models(extreme gradient boosting and random forest)to generate valid classifications of rockburst while producing a measure of confidence for its output.The proposed framework guarantees marginal coverage and,in most cases,conditional coverage on the test dataset.The CP was evaluated on a rockburst case in the Sanshandao Gold Mine in China,where it achieved high coverage and efficiency at applicable confidence levels.Significantly,the CP identified several“confident”classifications from the traditional ML model as unreliable,necessitating expert verification for informed decision-making.The proposed framework improves the reliability and accuracy of rockburst assessments,with the potential to bolster user confidence.展开更多
Implantable hydrogel-based bioelectronics(IHB)can precisely monitor human health and diagnose diseases.However,achieving biodegradability,biocompatibility,and high conformality with soft tissues poses significant chal...Implantable hydrogel-based bioelectronics(IHB)can precisely monitor human health and diagnose diseases.However,achieving biodegradability,biocompatibility,and high conformality with soft tissues poses significant challenges for IHB.Gelatin is the most suitable candidate for IHB since it is a collagen hydrolysate and a substantial part of the extracellular matrix found naturally in most tissues.This study used 3D printing ultrafine fiber networks with metamaterial design to embed into ultra-low elastic modulus hydrogel to create a novel gelatin-based conductive film(GCF)with mechanical programmability.The regulation of GCF nearly covers soft tissue mechanics,an elastic modulus from 20 to 420 kPa,and a Poisson’s ratio from-0.25 to 0.52.The negative Poisson’s ratio promotes conformality with soft tissues to improve the efficiency of biological interfaces.The GCF can monitor heartbeat signals and respiratory rate by determining cardiac deformation due to its high conformability.Notably,the gelatin characteristics of the biodegradable GCF enable the sensor to monitor and support tissue restoration.The GCF metamaterial design offers a unique idea for bioelectronics to develop implantable sensors that integrate monitoring and tissue repair and a customized method for endowing implanted sensors to be highly conformal with soft tissues.展开更多
The rapid advancement and broad application of machine learning(ML)have driven a groundbreaking revolution in computational biology.One of the most cutting-edge and important applications of ML is its integration with...The rapid advancement and broad application of machine learning(ML)have driven a groundbreaking revolution in computational biology.One of the most cutting-edge and important applications of ML is its integration with molecular simulations to improve the sampling efficiency of the vast conformational space of large biomolecules.This review focuses on recent studies that utilize ML-based techniques in the exploration of protein conformational landscape.We first highlight the recent development of ML-aided enhanced sampling methods,including heuristic algorithms and neural networks that are designed to refine the selection of reaction coordinates for the construction of bias potential,or facilitate the exploration of the unsampled region of the energy landscape.Further,we review the development of autoencoder based methods that combine molecular simulations and deep learning to expand the search for protein conformations.Lastly,we discuss the cutting-edge methodologies for the one-shot generation of protein conformations with precise Boltzmann weights.Collectively,this review demonstrates the promising potential of machine learning in revolutionizing our insight into the complex conformational ensembles of proteins.展开更多
Electromagnetic interference shielding(EMI SE)modules are the core com-ponent of modern electronics.However,the tra-ditional metal-based SE modules always take up indispensable three-dimensional space inside electroni...Electromagnetic interference shielding(EMI SE)modules are the core com-ponent of modern electronics.However,the tra-ditional metal-based SE modules always take up indispensable three-dimensional space inside electronics,posing a major obstacle to the integra-tion of electronics.The innovation of integrating 3D-printed conformal shielding(c-SE)modules with packaging materials onto core electronics offers infinite possibilities to satisfy ideal SE func-tion without occupying additional space.Herein,the 3D printable carbon-based inks with various proportions of graphene and carbon nanotube nanoparticles are well-formulated by manipulating their rheological peculiarity.Accordingly,the free-constructed architectures with arbitrarily-customized structure and multifunctionality are created via 3D printing.In particular,the SE performance of 3D-printed frame is up to 61.4 dB,simultaneously accompanied with an ultralight architecture of 0.076 g cm^(-3) and a superhigh specific shielding of 802.4 dB cm3 g^(-1).Moreover,as a proof-of-concept,the 3D-printed c-SE module is in situ integrated into core electronics,successfully replacing the traditional metal-based module to afford multiple functions for electromagnetic compatibility and thermal dissipa-tion.Thus,this scientific innovation completely makes up the blank for assembling carbon-based c-SE modules and sheds a brilliant light on developing the next generation of high-performance shielding materials with arbitrarily-customized structure for integrated electronics.展开更多
Although General Relativity is the classic example of a physical theory based on differential geometry, the momentum tensor is the only part of the field equation that is not derived from or interpreted with different...Although General Relativity is the classic example of a physical theory based on differential geometry, the momentum tensor is the only part of the field equation that is not derived from or interpreted with differential geometry. This work extends General Relativity and Einstein-Cartan theory by augmenting the Poincaré group with projective (special) conformal transformations, which are translations at conformal infinity. Momentum becomes a part of the differential geometry of spacetime. The Lie algebra of these transformations is represented by vectorfields on an associated Minkowski fiber space. Variation of projective conformal scalar curvature generates a 2-index tensor that serves as linear momentum in the field equations of General Relativity. The computation yields a constructive realization of Mach’s principle: local inertia is determined by local motion relative to mass at conformal infinity in each fiber. The vectorfields have a cellular structure that is similar to that of turbulent fluids.展开更多
We study nonhomogeneous systems of linear conformable fractional differential equations with pure delay.By using new conformable delayed matrix functions and the method of variation,we obtain a representation of their...We study nonhomogeneous systems of linear conformable fractional differential equations with pure delay.By using new conformable delayed matrix functions and the method of variation,we obtain a representation of their solutions.As an application,we derive a finite time stability result using the representation of solutions and a norm estimation of the conformable delayedmatrix functions.The obtained results are new,and they extend and improve some existing ones.Finally,an example is presented to illustrate the validity of our theoretical results.展开更多
The Laplace transformation is a very important integral transform,and it is extensively used in solving ordinary differential equations,partial differential equations,and several types of integro-differential equation...The Laplace transformation is a very important integral transform,and it is extensively used in solving ordinary differential equations,partial differential equations,and several types of integro-differential equations.Our purpose in this study is to introduce the notion of fuzzy double Laplace transform,fuzzy conformable double Laplace transform(FCDLT).We discuss some basic properties of FCDLT.We obtain the solutions of fuzzy partial differential equations(both one-dimensional and two-dimensional cases)through the double Laplace approach.We demonstrate through numerical examples that our proposed method is very successful and convenient for resolving partial differential equations.展开更多
This paper argues that the conformity-nonconformity dichotomy is a false dilemma.This study first critically reviews the basic philosophical,ethical,social psychological,and pedagogical literature related to the two c...This paper argues that the conformity-nonconformity dichotomy is a false dilemma.This study first critically reviews the basic philosophical,ethical,social psychological,and pedagogical literature related to the two concepts.It then outlines the way to overcome the phenomena of conformism and nonconformism together.The description of conformity and nonconformity as deprivation of freedom becomes stronger in 20th century philosophy and special literature from Heidegger through Fischer’s definition up to Cooley,and Crutchfield.Conformity is the sinking of the Self into the Anyone,the unprincipled alignment to the opinion of group mates,and nonconformity is the unprincipled resistance to it.But what is beyond conformity and nonconformity together as a group?There is a real community,in it the transformation of our pedagogical culture in a both useful and reasonable manner to allow the youth to accept the world by denying it and to deny the world by accepting it.The real community involves the virtue of goodness.Educate for goodness,because we possibly are the honest and humane man,who disregards the sinking of the self into the anyone and the self-contained rebellion.展开更多
最近,基于自注意力的Transformer结构在不同领域的一系列任务上表现出非常好的性能。探索了基于Transformer编码器和LAS(listen,attend and spell)解码器的Transformer-LAS语音识别模型的效果,并针对Transformer不善于捕捉局部信息的问...最近,基于自注意力的Transformer结构在不同领域的一系列任务上表现出非常好的性能。探索了基于Transformer编码器和LAS(listen,attend and spell)解码器的Transformer-LAS语音识别模型的效果,并针对Transformer不善于捕捉局部信息的问题,使用Conformer代替Transformer,提出Conformer-LAS模型。由于Attention过于灵活的对齐方式,使得在嘈杂环境中的效果急剧下降,采用连接时序分类(connectionist temporal classification,CTC)辅助训练以加快收敛,并加入音素级别的中间CTC损失联合优化,提出了效果更好的Conformer-LAS-CTC语音识别模型。在开源中文普通话Aishell-1数据集上对提出来的模型进行验证,实验结果表明,Conformer-LAS-CTC相对于采用的基线BLSTM-LAS和Transformer-LAS模型在测试集上的字错率分别相对降低了22.58%和48.76%,模型最终字错误率为4.54%。展开更多
This article studies a nonlinear fractional order Lotka-Volterra prey-predator type dynamical system.For the proposed study,we consider the model under the conformable fractional order derivative(CFOD).We investigate ...This article studies a nonlinear fractional order Lotka-Volterra prey-predator type dynamical system.For the proposed study,we consider the model under the conformable fractional order derivative(CFOD).We investigate the mentioned dynamical system for the existence and uniqueness of at least one solution.Indeed,Schauder and Banach fixed point theorems are utilized to prove our claim.Further,an algorithm for the approximate analytical solution to the proposed problem has been established.In this regard,the conformable fractional differential transform(CFDT)technique is used to compute the required results in the form of a series.Using Matlab-16,we simulate the series solution to illustrate our results graphically.Finally,a comparison of our solution to that obtained for the Caputo fractional order derivative via the perturbation method is given.展开更多
针对使用Conformer模型的语音识别算法在实际应用时设备算力不足及资源缺乏的问题,提出一种基于Conformer模型间隔剪枝和参数量化相结合的模型压缩方法。实验显示,使用该方法压缩后,模型的实时率(real time factor, RTF)达到0.107614,...针对使用Conformer模型的语音识别算法在实际应用时设备算力不足及资源缺乏的问题,提出一种基于Conformer模型间隔剪枝和参数量化相结合的模型压缩方法。实验显示,使用该方法压缩后,模型的实时率(real time factor, RTF)达到0.107614,较基线模型的推理速度提升了16.2%,而识别准确率只下降了1.79%,并且模型大小也由原来的207.91MB下降到72.69MB。该方法在模型准确率损失很小的情况下,较大程度地提升了模型的适用性。展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation(2021B15151300042021B1515140021)+2 种基金the Scientific Research Start-up Funding of Guangdong Medical University(1026/4SG21229G)China Postdoctoral Science Foundation(2021M702781)Guangdong Medical University Post-doctoral Research Funding(2BH19006P)。
文摘Ovalbumin(OVA)is the major allergenic protein that can induce T helper 2(Th2)-allergic reactions,for which current treatment options are inadequate.In this study,we developed a polymerized hypoallergenic OVA product via laccase/caffeic acid(Lac/CA)-catalyzed crosslinking in conjunction with galactomannan(Man).The formation of high molecular weight crosslinked polymers and the Ig G-binding were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting.The study indicated that Lac/CA-catalyzed crosslinking plus Man conjugation substantially altered secondary and tertiary structures of OVA along with the variation in surface hydrophobicity.Gastrointestinal digestion stability assay indicated that crosslinked OVA exhibited less resistance in simulated gastric fluid(SGF)and simulated intestinal fluid(SIF).Mouse model study indicated that Lac-Man/OVA ameliorated eosinophilic airway inflammatory response and efficiently downregulated the expression of Th2-related cytokines(interleukin(IL)-4,IL-5,and IL-13),and upregulated IFN-γand IL-10 expression.Stimulation of bone marrow-derived dendritic cells with Lac-Man/OVA suppressed the expression of phenotypic maturation markers(CD80 and CD86)and MHC class II molecules,and suppressed the expression levels of proinflammatory cytokines.The knowledge obtained in the present study offers an effective way to acquire a hypoallergenic OVA product that can have a therapeutic effect in alleviating OVA-induced allergic asthma.
文摘The scientific community recognizes the seriousness of rockbursts and the need for effective mitigation measures.The literature reports various successful applications of machine learning(ML)models for rockburst assessment;however,a significant question remains unanswered:How reliable are these models,and at what confidence level are classifications made?Typically,ML models output single rockburst grade even in the face of intricate and out-of-distribution samples,without any associated confidence value.Given the susceptibility of ML models to errors,it becomes imperative to quantify their uncertainty to prevent consequential failures.To address this issue,we propose a conformal prediction(CP)framework built on traditional ML models(extreme gradient boosting and random forest)to generate valid classifications of rockburst while producing a measure of confidence for its output.The proposed framework guarantees marginal coverage and,in most cases,conditional coverage on the test dataset.The CP was evaluated on a rockburst case in the Sanshandao Gold Mine in China,where it achieved high coverage and efficiency at applicable confidence levels.Significantly,the CP identified several“confident”classifications from the traditional ML model as unreliable,necessitating expert verification for informed decision-making.The proposed framework improves the reliability and accuracy of rockburst assessments,with the potential to bolster user confidence.
基金This work was sponsored by the National Natural Science Foundation of China(No.52235007,52325504)the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(No.T2121004).
文摘Implantable hydrogel-based bioelectronics(IHB)can precisely monitor human health and diagnose diseases.However,achieving biodegradability,biocompatibility,and high conformality with soft tissues poses significant challenges for IHB.Gelatin is the most suitable candidate for IHB since it is a collagen hydrolysate and a substantial part of the extracellular matrix found naturally in most tissues.This study used 3D printing ultrafine fiber networks with metamaterial design to embed into ultra-low elastic modulus hydrogel to create a novel gelatin-based conductive film(GCF)with mechanical programmability.The regulation of GCF nearly covers soft tissue mechanics,an elastic modulus from 20 to 420 kPa,and a Poisson’s ratio from-0.25 to 0.52.The negative Poisson’s ratio promotes conformality with soft tissues to improve the efficiency of biological interfaces.The GCF can monitor heartbeat signals and respiratory rate by determining cardiac deformation due to its high conformability.Notably,the gelatin characteristics of the biodegradable GCF enable the sensor to monitor and support tissue restoration.The GCF metamaterial design offers a unique idea for bioelectronics to develop implantable sensors that integrate monitoring and tissue repair and a customized method for endowing implanted sensors to be highly conformal with soft tissues.
基金Project supported by the National Key Research and Development Program of China(Grant No.2023YFF1204402)the National Natural Science Foundation of China(Grant Nos.12074079 and 12374208)+1 种基金the Natural Science Foundation of Shanghai(Grant No.22ZR1406800)the China Postdoctoral Science Foundation(Grant No.2022M720815).
文摘The rapid advancement and broad application of machine learning(ML)have driven a groundbreaking revolution in computational biology.One of the most cutting-edge and important applications of ML is its integration with molecular simulations to improve the sampling efficiency of the vast conformational space of large biomolecules.This review focuses on recent studies that utilize ML-based techniques in the exploration of protein conformational landscape.We first highlight the recent development of ML-aided enhanced sampling methods,including heuristic algorithms and neural networks that are designed to refine the selection of reaction coordinates for the construction of bias potential,or facilitate the exploration of the unsampled region of the energy landscape.Further,we review the development of autoencoder based methods that combine molecular simulations and deep learning to expand the search for protein conformations.Lastly,we discuss the cutting-edge methodologies for the one-shot generation of protein conformations with precise Boltzmann weights.Collectively,this review demonstrates the promising potential of machine learning in revolutionizing our insight into the complex conformational ensembles of proteins.
基金This work is financially supported by the National Natural Science Foundation of China(52303036)the Natural Science Foundation of Guangxi Province(2020GXNSFAA297028)+4 种基金the Guangxi Science and Technology Base and Talent Special Project(GUIKE AD23026179)the International Science&Technology Cooperation Project of Chengdu(2021-GH03-00009-HZ)the Program of Innovative Research Team for Young Scientists of Sichuan Province(22CXTD0019)the Natural Science Foundation of Sichuan Province(2023NSFSC0986)the Opening Project of State Key Laboratory of Polymer Materials Engineering(Sichuan University)(Sklpme2023-3-18).
文摘Electromagnetic interference shielding(EMI SE)modules are the core com-ponent of modern electronics.However,the tra-ditional metal-based SE modules always take up indispensable three-dimensional space inside electronics,posing a major obstacle to the integra-tion of electronics.The innovation of integrating 3D-printed conformal shielding(c-SE)modules with packaging materials onto core electronics offers infinite possibilities to satisfy ideal SE func-tion without occupying additional space.Herein,the 3D printable carbon-based inks with various proportions of graphene and carbon nanotube nanoparticles are well-formulated by manipulating their rheological peculiarity.Accordingly,the free-constructed architectures with arbitrarily-customized structure and multifunctionality are created via 3D printing.In particular,the SE performance of 3D-printed frame is up to 61.4 dB,simultaneously accompanied with an ultralight architecture of 0.076 g cm^(-3) and a superhigh specific shielding of 802.4 dB cm3 g^(-1).Moreover,as a proof-of-concept,the 3D-printed c-SE module is in situ integrated into core electronics,successfully replacing the traditional metal-based module to afford multiple functions for electromagnetic compatibility and thermal dissipa-tion.Thus,this scientific innovation completely makes up the blank for assembling carbon-based c-SE modules and sheds a brilliant light on developing the next generation of high-performance shielding materials with arbitrarily-customized structure for integrated electronics.
文摘Although General Relativity is the classic example of a physical theory based on differential geometry, the momentum tensor is the only part of the field equation that is not derived from or interpreted with differential geometry. This work extends General Relativity and Einstein-Cartan theory by augmenting the Poincaré group with projective (special) conformal transformations, which are translations at conformal infinity. Momentum becomes a part of the differential geometry of spacetime. The Lie algebra of these transformations is represented by vectorfields on an associated Minkowski fiber space. Variation of projective conformal scalar curvature generates a 2-index tensor that serves as linear momentum in the field equations of General Relativity. The computation yields a constructive realization of Mach’s principle: local inertia is determined by local motion relative to mass at conformal infinity in each fiber. The vectorfields have a cellular structure that is similar to that of turbulent fluids.
文摘We study nonhomogeneous systems of linear conformable fractional differential equations with pure delay.By using new conformable delayed matrix functions and the method of variation,we obtain a representation of their solutions.As an application,we derive a finite time stability result using the representation of solutions and a norm estimation of the conformable delayedmatrix functions.The obtained results are new,and they extend and improve some existing ones.Finally,an example is presented to illustrate the validity of our theoretical results.
基金Manar A.Alqudah would like to thank Princess Nourah bint Abdulrahman University Researchers Supporting Project No.(PNURSP2022R14),Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia。
文摘The Laplace transformation is a very important integral transform,and it is extensively used in solving ordinary differential equations,partial differential equations,and several types of integro-differential equations.Our purpose in this study is to introduce the notion of fuzzy double Laplace transform,fuzzy conformable double Laplace transform(FCDLT).We discuss some basic properties of FCDLT.We obtain the solutions of fuzzy partial differential equations(both one-dimensional and two-dimensional cases)through the double Laplace approach.We demonstrate through numerical examples that our proposed method is very successful and convenient for resolving partial differential equations.
文摘This paper argues that the conformity-nonconformity dichotomy is a false dilemma.This study first critically reviews the basic philosophical,ethical,social psychological,and pedagogical literature related to the two concepts.It then outlines the way to overcome the phenomena of conformism and nonconformism together.The description of conformity and nonconformity as deprivation of freedom becomes stronger in 20th century philosophy and special literature from Heidegger through Fischer’s definition up to Cooley,and Crutchfield.Conformity is the sinking of the Self into the Anyone,the unprincipled alignment to the opinion of group mates,and nonconformity is the unprincipled resistance to it.But what is beyond conformity and nonconformity together as a group?There is a real community,in it the transformation of our pedagogical culture in a both useful and reasonable manner to allow the youth to accept the world by denying it and to deny the world by accepting it.The real community involves the virtue of goodness.Educate for goodness,because we possibly are the honest and humane man,who disregards the sinking of the self into the anyone and the self-contained rebellion.
文摘最近,基于自注意力的Transformer结构在不同领域的一系列任务上表现出非常好的性能。探索了基于Transformer编码器和LAS(listen,attend and spell)解码器的Transformer-LAS语音识别模型的效果,并针对Transformer不善于捕捉局部信息的问题,使用Conformer代替Transformer,提出Conformer-LAS模型。由于Attention过于灵活的对齐方式,使得在嘈杂环境中的效果急剧下降,采用连接时序分类(connectionist temporal classification,CTC)辅助训练以加快收敛,并加入音素级别的中间CTC损失联合优化,提出了效果更好的Conformer-LAS-CTC语音识别模型。在开源中文普通话Aishell-1数据集上对提出来的模型进行验证,实验结果表明,Conformer-LAS-CTC相对于采用的基线BLSTM-LAS和Transformer-LAS模型在测试集上的字错率分别相对降低了22.58%和48.76%,模型最终字错误率为4.54%。
文摘This article studies a nonlinear fractional order Lotka-Volterra prey-predator type dynamical system.For the proposed study,we consider the model under the conformable fractional order derivative(CFOD).We investigate the mentioned dynamical system for the existence and uniqueness of at least one solution.Indeed,Schauder and Banach fixed point theorems are utilized to prove our claim.Further,an algorithm for the approximate analytical solution to the proposed problem has been established.In this regard,the conformable fractional differential transform(CFDT)technique is used to compute the required results in the form of a series.Using Matlab-16,we simulate the series solution to illustrate our results graphically.Finally,a comparison of our solution to that obtained for the Caputo fractional order derivative via the perturbation method is given.
文摘针对使用Conformer模型的语音识别算法在实际应用时设备算力不足及资源缺乏的问题,提出一种基于Conformer模型间隔剪枝和参数量化相结合的模型压缩方法。实验显示,使用该方法压缩后,模型的实时率(real time factor, RTF)达到0.107614,较基线模型的推理速度提升了16.2%,而识别准确率只下降了1.79%,并且模型大小也由原来的207.91MB下降到72.69MB。该方法在模型准确率损失很小的情况下,较大程度地提升了模型的适用性。