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沙眼衣原体蛋白酶CPAF的结构特征及抗原表位分析 被引量:1
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作者 王道 张宏波 +1 位作者 刘文彬 陈建林 《激光生物学报》 CAS 2023年第3期247-258,271,共13页
衣原体蛋白酶样活性因子(CPAF)是一种沙眼衣原体蛋白酶,其活性可抑制宿主炎症信号、细胞骨架重塑和中性粒细胞活化等过程。本研究运用生物信息学方法对CPAF蛋白的理化性质、亲/疏水性、信号肽、亚细胞定位、跨膜结构、保守结构域、N-糖... 衣原体蛋白酶样活性因子(CPAF)是一种沙眼衣原体蛋白酶,其活性可抑制宿主炎症信号、细胞骨架重塑和中性粒细胞活化等过程。本研究运用生物信息学方法对CPAF蛋白的理化性质、亲/疏水性、信号肽、亚细胞定位、跨膜结构、保守结构域、N-糖基化和磷酸化位点、二级/三级结构、配体结合区域和小分子药物、B/T细胞抗原表位以及相互作用蛋白等进行分析。CPAF由601个氨基酸组成,分子质量为67252.57Da,理论等电点为5.68,为不稳定的亲水性蛋白质;含有信号肽,无跨膜区,保守结构域位于3~586位氨基酸序列,属于CPAF超家族,主要分布在细胞质;分别存在1个N-糖基化和53个磷酸化位点,二级结构主要以无规则卷曲和α-螺旋组成,存在14个配体结合位点、20个B细胞抗原表位、4种B细胞构象抗原表位、多个可能的T细胞抗原表位。此外,本文还虚拟筛选出5种潜在的有效小分子化合物,CPAF能与htrA、pbpB、recC等10种其他蛋白发生互作关系。这为深入研究CPAF蛋白在沙眼衣原体发病机制中的重要作用提供了理论依据,使其可能成为疫苗研发和药物治疗的靶点。 展开更多
关键词 沙眼衣原体 cpaf蛋白 生物信息学 结构 抗原表位
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肺炎嗜衣原体CPAF重组蛋白致小鼠肺组织炎症及对炎症细胞因子的影响 被引量:10
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作者 王华丽 吴移谋 +4 位作者 郑江花 朱翠明 李忠玉 周洲 余敏君 《中国免疫学杂志》 CAS CSCD 北大核心 2011年第4期308-311,共4页
目的:研究肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)蛋白酶样活性因子(CPAF)的181400aa基因(CPAFm)的重组蛋白在BALB/c小鼠体内引起的肺部炎症改变及对炎症细胞因子TNF-α和IL-6的水平的影响,为进一步探索CPAF在Cpn感染中的... 目的:研究肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)蛋白酶样活性因子(CPAF)的181400aa基因(CPAFm)的重组蛋白在BALB/c小鼠体内引起的肺部炎症改变及对炎症细胞因子TNF-α和IL-6的水平的影响,为进一步探索CPAF在Cpn感染中的致病作用提供实验依据。方法:将纯化的CPAFm重组蛋白于鼻内或尾静脉注射BALB/c小鼠,HE染色观察肺组织病理形态学改变,计小鼠外周血与支气管肺泡灌洗液(Bronchoalveolar lavage fluids,BALF)中白细胞总数,ELISA方法检测血清和BALF中的TNFα-和IL-6的水平。结果:实验组BALB/c小鼠的肺组织出现中性粒细胞、淋巴细胞等炎症细胞浸润,对照组与正常组BALB/c小鼠肺组织未见明显病理改变。鼻内滴入重组蛋白实验组BALB/c小鼠BALF中白细胞总数明显高于GST对照组(P〈0.05)、PBS对照组(P〈0.05)和正常组(P〈0.01);鼻内滴入和尾静脉注射重组蛋白实验组BALF中炎症因子IL-6、TNF-α的水平明显高于GST、PBS对照组与正常组(均P〈0.01);鼻内滴入重组蛋白实验组外周血中炎症因子IL-6和TNF-α水平显著高于GST、PBS对照组和正常组(P〈0.05);尾静脉注射重组蛋白实验组外周血中炎症因子IL-6的水平明显高于相应的对照组(均P〈0.01)。结论:CPAFm重组蛋白有致病性,能引起BALB/c小鼠肺组织的炎症损伤和炎症因子TNF-α、IL-6的水平的升高,肺损伤与BALB/c小鼠体内炎症细胞增多和炎症因子TNF-α、IL-6的水平的升高相关。 展开更多
关键词 肺炎嗜衣原体 重组蛋白 cpaf 炎症细胞因子
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肺炎嗜衣原体CPAF原核表达载体的构建及其在大肠杆菌中的高效表达 被引量:1
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作者 周洲 吴移谋 +2 位作者 陈丽丽 李忠玉 陈超群 《中南医学科学杂志》 CAS 2011年第6期615-618,共4页
目的对肺炎嗜衣原体蛋白酶样活性因子(CPAF)基因进行原核表达,为肺炎嗜衣原体的诊断及疫苗研究奠定基础。方法利用PCR技术从肺炎嗜衣原体标准株AR-39中扩增CPAF基因,将其克隆于原核表达载体pGEX-6p-2中,构建重组表达载体。并用双酶切、... 目的对肺炎嗜衣原体蛋白酶样活性因子(CPAF)基因进行原核表达,为肺炎嗜衣原体的诊断及疫苗研究奠定基础。方法利用PCR技术从肺炎嗜衣原体标准株AR-39中扩增CPAF基因,将其克隆于原核表达载体pGEX-6p-2中,构建重组表达载体。并用双酶切、PCR及序列测定等方法进行鉴定后,在大肠杆菌中诱导表达GST融合蛋白。结果重组质粒中CPAF基因序列与肺炎嗜衣原体AR-39的同源性达到100%,CPAF在大肠杆菌中表达高效的GST融合蛋白。结论成功构建肺炎嗜衣原体CPAF基因的原核表达系统,并成功表达重组蛋白,为肺炎嗜衣原体疫苗的研究和临床检测试剂盒的研制打下基础。 展开更多
关键词 肺炎嗜衣原体 cpaf基因 原核表达
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衣原体蛋白酶样活性因子(CPAF)的克隆、表达及纯化
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作者 王飞 朱庆三 +3 位作者 苗旭漫 胡宁宁 李霄 金宁一 《中国老年学杂志》 CAS CSCD 北大核心 2010年第5期635-638,共4页
目的构建含衣原体蛋白酶样活性因子(CPAF)的原核表达载体,经转化E.coli以表达CPAF融合蛋白,研究其生物学功能。方法从病料中克隆得到CPAF的原始基因,将PCR产物经纯化连接到pMD18-T,将重组质粒pMD-CPAF经NdeI和BamHI双酶切,与用相同酶消... 目的构建含衣原体蛋白酶样活性因子(CPAF)的原核表达载体,经转化E.coli以表达CPAF融合蛋白,研究其生物学功能。方法从病料中克隆得到CPAF的原始基因,将PCR产物经纯化连接到pMD18-T,将重组质粒pMD-CPAF经NdeI和BamHI双酶切,与用相同酶消化的原核表达载体pET42b(+)连接,建立pET42b(+)-CPAF表达载体,阳性克隆经鉴定后,转化大肠杆菌BL21(DE3)进行表达,经亲和纯化、离子交换纯化、分子筛纯化融合蛋白,进行SDS-PAGE分析,Western印迹检测。结果经酶切鉴定和测序分析证实原核表达质粒构建正确。SDS-PAGE和West-ern印迹结果显示在70kD处呈现单一蛋白条带,pET42b(+)-CPAF重组载体在大肠杆菌BL21(DE3)中成功地表达了CPAF天然蛋白,目的蛋白占全菌体蛋白的20%左右,采用镍柱的亲和纯化后,可达70%左右。结论经DNA测序、SDS-PAGE、Western印迹检测表明成功构建了能够稳定表达可溶性CPAF的菌株,并获得纯化CPAF的方法,为今后CPAF的研究及应用奠定了基础。 展开更多
关键词 衣原体蛋白酶样活性因子(cpaf) 表达 纯化 生物学功能
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山羊流产衣原体ompA和CPAF基因的序列分析及原核表达 被引量:1
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作者 徐海玲 杨萍 +5 位作者 张金生 王满定 刘广阔 李梦磊 张琪 许信刚 《动物医学进展》 北大核心 2022年第2期1-6,共6页
为了解山羊流产衣原体陕西分离株ompA和CPAF基因的遗传变异情况及制备检测抗原,根据GenBank收录的ompA和CPAF基因序列设计合成特异性引物,分别进行ompA基因和CPAF基因的基因克隆、序列分析及原核表达。结果表明,成功克隆出大小为1053 bp... 为了解山羊流产衣原体陕西分离株ompA和CPAF基因的遗传变异情况及制备检测抗原,根据GenBank收录的ompA和CPAF基因序列设计合成特异性引物,分别进行ompA基因和CPAF基因的基因克隆、序列分析及原核表达。结果表明,成功克隆出大小为1053 bp的ompA基因和大小为1056 bp的CPAF基因;核酸序列分析显示ompA和CPAF基因与已报道的山羊流产衣原体ompA和CPAF基因同源性分别为99.54%~99.91%和91%~100%;成功构建了表达ompA和CPAF基因的原核表达菌株,经IPTG诱导后分别表达38.6 ku和38.7 ku的ompA和CPAF重组蛋白,Western blot显示纯化复性的两种重组蛋白均能与山羊流产衣原体阳性血清相结合。试验结果为进一步建立山羊流产衣原体抗体ELISA检测方法奠定了基础。 展开更多
关键词 山羊流产衣原体 ompA基因 cpaf基因 序列分析 原核表达
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流产衣原体CPAF基因的克隆序列分析及原核表达
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作者 刘萍 马晓霞 +5 位作者 周小凯 马鹏 常秋燕 李林杰 李凌浩 马忠仁 《华北农学报》 CSCD 北大核心 2018年第3期86-91,共6页
为了研究流产衣原体蛋白酶样活性因子(CPAF)的免疫原性,建立快速有效的流产衣原体抗体检测方法,根据Gen Bank中公布的流产衣原体基因组序列,设计并合成了流产衣原体CPAF蛋白编码基因的特异性引物,以流产衣原体标准菌株基因组为模板,经PC... 为了研究流产衣原体蛋白酶样活性因子(CPAF)的免疫原性,建立快速有效的流产衣原体抗体检测方法,根据Gen Bank中公布的流产衣原体基因组序列,设计并合成了流产衣原体CPAF蛋白编码基因的特异性引物,以流产衣原体标准菌株基因组为模板,经PCR扩增获得长度为1 809 bp目的基因片段;对获得的目的基因进行测序,并用生物信息学软件进行预测分析;再将该片段插入原核表达载体p ET-30a中,经双酶切、测序鉴定;构建成功的表达质粒转化大肠杆菌BL21(DE3),并用IPTG对目的蛋白进行诱导表达,通过对诱导条件的优化,确定诱导表达的最佳条件,表达产物用SDS-PAGE和Western Blot检测。结果显示,成功克隆流产衣原体CPAF蛋白的编码基因,该编码产物可能是定位于宿主细胞质中的一种衣原体分泌性蛋白酶,分子内具有多个可能的抗原表位;重组表达质粒经IPTG诱导后,表达分子质量为67.6 ku的CPAF蛋白,该重组蛋白能与抗His-tag单克隆抗体以及流产衣原体阳性血清特异性结合,具有良好的反应原性。结果表明,以大肠杆菌表达系统重组表达的流产衣原体CPAF蛋白具有良好的免疫原性,可作为动物流产衣原体病的血清学诊断及亚单位疫苗研究的候选抗原。 展开更多
关键词 流产衣原体 cpaf 克隆 序列分析 原核表达
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不同基因型沙眼衣原体小鼠生殖道感染中CPAF、IFN-γ和IL-10的表达 被引量:2
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作者 王柳苑 赵培祯 +3 位作者 薛耀华 杨立刚 郑和平 杨斌 《皮肤性病诊疗学杂志》 2019年第4期199-203,共5页
目的:建立不同基因型沙眼衣原体小鼠生殖道感染模型,分析CPAF、IFN-γ和IL-10在不同基因型沙眼衣原体小鼠生殖道感染中表达的差异。方法:将70只雌性Balb/c小鼠随机分为E组、F组、J组、K组、H组、空白对照组及实验对照组,阴道内分别接种3... 目的:建立不同基因型沙眼衣原体小鼠生殖道感染模型,分析CPAF、IFN-γ和IL-10在不同基因型沙眼衣原体小鼠生殖道感染中表达的差异。方法:将70只雌性Balb/c小鼠随机分为E组、F组、J组、K组、H组、空白对照组及实验对照组,阴道内分别接种30μL E、F、J、K、H型沙眼衣原体,空白对照组不做处理,实验对照组阴道接种30μL SPG。ELISA法检测感染后第35 d子宫输卵管组织匀浆中CPAF、IFN-γ和IL-10的表达,并进行统计比较。结果:接种CT后35 d:H组子宫输卵管组织匀浆CPAF浓度明显高于E组、F组、J组(P值均<0.05),K组CPAF浓度明显高于F组(P<0.05);H组和K组子宫输卵管组织匀浆IFN-γ和IL-10浓度均明显高于E组、F组、J组(P值均<0.05)。结论:不同型别CT感染小鼠后,上生殖道组织中CPAF、IFN-γ和IL-10呈明显差异表达,提示不同基因型CT泌尿生殖道感染可能产生不同的临床结局。 展开更多
关键词 沙眼衣原体 cpaf IFN-Γ IL-10 小鼠
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CPAFFC President Lin Songtian meets new Russian ambassador
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作者 Feng Miao 《Voice of Friendship》 2023年第1期9-10,共2页
On Feb 6, Lin Songtian, president of the Chinese People’s Association for Friendship with Foreign Countries, met with new Russian Ambassador to China Igor Morgulov and his party and hosted a luncheon at the CPAFFC’s... On Feb 6, Lin Songtian, president of the Chinese People’s Association for Friendship with Foreign Countries, met with new Russian Ambassador to China Igor Morgulov and his party and hosted a luncheon at the CPAFFC’s compound in Beijing. 展开更多
关键词 RUSSIAN Beijing. cpaf
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CPAFFC President Lin Songtian receives warm welcome in San Francisco
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作者 《Voice of Friendship》 2023年第1期5-6,共2页
On Feb 19,Lin Songtian,president of the CPAFFC, and his party flew to San Francisco from Washington and held meetings with David Haubert, honorary chair of the Sino-American Friendship City Association and supervisor ... On Feb 19,Lin Songtian,president of the CPAFFC, and his party flew to San Francisco from Washington and held meetings with David Haubert, honorary chair of the Sino-American Friendship City Association and supervisor of Alameda County, California, and Jonathan Ginsberg, executive director of the Sino-American Friendship City Association. 展开更多
关键词 cpaf WELCOME HONOR
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肺炎嗜衣原体CPAF诱导THP-1细胞产生前炎症细胞因子和凋亡 被引量:1
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作者 胡旃 吴移谋 +3 位作者 陈虹亮 郑江花 周洲 唐国芳 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2010年第5期487-492,共6页
目的 研究肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)衣原体蛋白酶样活性因子(Chlamydial protease-like activity factor,CPAF)能否在体外诱导人单核细胞THP-1产生前炎症细胞因子和凋亡,为进一步探索Cpn感染宿主致病的分子机制... 目的 研究肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)衣原体蛋白酶样活性因子(Chlamydial protease-like activity factor,CPAF)能否在体外诱导人单核细胞THP-1产生前炎症细胞因子和凋亡,为进一步探索Cpn感染宿主致病的分子机制提供实验依据.方法 将Cpn CPAF全基因克隆于pGEX6p-2载体上,在大肠杆菌(E coli)中诱导表达重组蛋白,经去内毒素纯化柱和琼脂糖凝胶FF获得纯化且无脂多糖(LPS)污染的重组蛋白GST-CPAF.以不同浓度的该蛋白作用于THP-1,ELISA检测TNF-α吨和IL-6水平.MTT法检测经该蛋白处理后THP-1的增生或抑制作用,用Hoechst33258荧光染色、DNA片段化分析、Armexin V-FITC-PI染色法检测细胞凋亡情况.结果 制备的莺组蛋白GST-CPAF以时间和剂量依赖方式刺激THP-1分泌TNF-α和IL-6,并以剂量依赖方式抑制THP-1增殖;当GST-CPAF刺激THP-1细胞24 h后,能诱导细胞调亡.结论 制备的Cpn重组蛋白GST-CPAF能诱导THP-1产生前炎症细胞因子和凋亡,可能是Cpn致病机制中的一个因素. 展开更多
关键词 肺炎嗜衣原体 cpaf THP-1 前炎症细胞因子 细胞凋亡
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CPAFFC President Lin Songtian meets Kazakhstan ambassador
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作者 Cai Yunzhe 《Voice of Friendship》 2022年第3期20-21,共2页
On June 22,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with Nuryshev Shakhrat,Kazakhstan’s new ambassador to China.The two sides had candid and in-depth excha... On June 22,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with Nuryshev Shakhrat,Kazakhstan’s new ambassador to China.The two sides had candid and in-depth exchanges on China-Kazakhstan relations and peopleto-people friendship and cooperation.Hao Zhongwei,deputy directorgeneral of the CPAFFC’s department of European and Asian affairs was also present at the meeting. 展开更多
关键词 bass cpaf AFFAIRS
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CPAFFC President Lin Songtian meets pianist Claudia Yang
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作者 Hou Mengwei 《Voice of Friendship》 2022年第3期58-58,共1页
On June 17,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with Claudia Yang,international art director of the Silk Road International Arts Center and a wellknown ... On June 17,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with Claudia Yang,international art director of the Silk Road International Arts Center and a wellknown Malaysian Chinese pianist.Zhu Dan,director-general of the CPAFFC’s Cultural Exchange Department,and Wang Longshe,secretary-general of the China Friendship Foundation for Peace and Development,attended the meeting. 展开更多
关键词 PEACE DIRECTOR cpaf
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CPAFFC President Lin Songtian meets Uzbekistan ambassador
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作者 Cai Yunzhe 《Voice of Friendship》 2022年第3期12-13,共2页
On June 14,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with Farhod Arziev,Uzbekistan’s new ambassador to China.The two sides had candid and in-depth exchanges... On June 14,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with Farhod Arziev,Uzbekistan’s new ambassador to China.The two sides had candid and in-depth exchanges on the development of China-Uzbekistan relations and peopleto-people friendship and cooperation.Hao Zhongwei,deputy directorgeneral of the CPAFFC’s department of European and Asian affairs,attended the meeting. 展开更多
关键词 bass cpaf AFFAIRS
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CPAFFC President Lin Songtian meets Rwanda ambassador
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作者 Zhang Peng 《Voice of Friendship》 2022年第2期19-20,共2页
On Apr 21,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with James Kimonyo,Rwanda’s ambassador to China.They had in-depth exchanges on bilateral and China-Afric... On Apr 21,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with James Kimonyo,Rwanda’s ambassador to China.They had in-depth exchanges on bilateral and China-Africa relations,as well as on international issues of common concern.They reached broad consensus.Sun Xueqing,director-general of the CPAFFC’s department of Asian and African affairs,also attended the meeting. 展开更多
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CPAFFC Vice-President Li Xikui attends opening ceremony of First International Intangible Cultural Heritage Week
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作者 Gan Qianyi 《Voice of Friendship》 2022年第3期40-41,共2页
On Aug 9,the First International Intangible Cultural Heritage Week(Weinan,Shaanxi)opened in Weinan,Shaanxi province.It was co-hosted by the Chinese People’s Association for Friendship with Foreign Countries and the S... On Aug 9,the First International Intangible Cultural Heritage Week(Weinan,Shaanxi)opened in Weinan,Shaanxi province.It was co-hosted by the Chinese People’s Association for Friendship with Foreign Countries and the Shaanxi Provincial People’s Government.Li Xikui,vice-president of the CPAFFC;Ye Niuping,vicegovernor of Shaanxi province;Fan Weibin,municipal Party secretary of Weinan;and Shahbaz Khan,UNESCO representative in China,attended and spoke at the opening ceremony. 展开更多
关键词 UNESCO CEREMONY cpaf
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CPAFFC Vice-President Jiang Jiang delivers a video speech for the exchange activity between Heilongjiang province and its international friendship cities
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作者 Sun Liang 《Voice of Friendship》 2022年第1期38-39,共2页
On Jan 20,an exchange activity between Heilongjiang province and its international friendship cities was held in Harbin,online and offline simultaneously.The theme of the activity was“Holding hands with friendship ci... On Jan 20,an exchange activity between Heilongjiang province and its international friendship cities was held in Harbin,online and offline simultaneously.The theme of the activity was“Holding hands with friendship cities and meeting at the Winter Olympics”.Jiang Jiang,vice-president of the CPAFFC,delivered a video speech and offered congratulations for the activity’s success. 展开更多
关键词 FRIENDS Heilongjiang cpaf
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Ambassadors of Russia and five Central Asian countries visit CPAFFC
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作者 Cai Yunzhe 《Voice of Friendship》 2021年第3期15-16,共2页
On July 13,at the invitation of CPAFFC President Lin Songtian,Russian Ambassador Andrey Denisov,Uzbek Ambassador Bakhtiyor Saidov,Kyrgyz Ambassador Kanaiym Baktygulova,Turkmen Ambassador Parak hat H.Durdyev,Kazakh Amb... On July 13,at the invitation of CPAFFC President Lin Songtian,Russian Ambassador Andrey Denisov,Uzbek Ambassador Bakhtiyor Saidov,Kyrgyz Ambassador Kanaiym Baktygulova,Turkmen Ambassador Parak hat H.Durdyev,Kazakh Ambassador Gabit Koishibayev and Tajik Ambassador Saidzoda Zohir Ozod visited the CPAFFC’s headquarters in Beijing. 展开更多
关键词 AMBASSADOR cpaf RUSSIA
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Cuban government confers Order of Solidarity on CPAFFC
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作者 Chen Kuanghuai 《Voice of Friendship》 2022年第4期50-51,共2页
On Nov 1,the embassy of Cuba in China held a grand ceremony to confer the Order of Solidarity on the Chinese People’s Association for Friendship with Foreign Countries on behalf of the Cuban government.Lin Songtian,p... On Nov 1,the embassy of Cuba in China held a grand ceremony to confer the Order of Solidarity on the Chinese People’s Association for Friendship with Foreign Countries on behalf of the Cuban government.Lin Songtian,president of the CPAFFC,attended the ceremony.Cuban Ambassador to China Carlos Miguel Pereira presented the order to Lin and made a speech.Fernando González,president of Cuban Institute of Friendship with the Peoples,delivered a video message. 展开更多
关键词 behalf CEREMONY cpaf
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CPAFFC President Lin Songtian:Carry forward the Olympic spirit,deepen cooperation between friendship cities
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作者 Chen Ke 《Voice of Friendship》 2022年第1期32-33,共2页
On Jan 14,CPAFFC President Lin Songtian gave an exclusive interview on CCTV’s Chinese International Channel and the CCTV News Channel on the significance and main features of the Beijing Winter Olympics Friendship Ci... On Jan 14,CPAFFC President Lin Songtian gave an exclusive interview on CCTV’s Chinese International Channel and the CCTV News Channel on the significance and main features of the Beijing Winter Olympics Friendship Cities Cooperation and Development Forum,its theme and the Olympic spirit and people-to-people ties,among other topics.Lin said the success of the 2008 Beijing Olympics left the world with a splendid,deep impression. 展开更多
关键词 INTERVIEW SPIRIT cpaf
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Former CPAFFC president’s farewell to friends around the world
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作者 Li Xiaolin 《Voice of Friendship》 2020年第1期3-3,共1页
Dear Friends,I’m hereby informing you that His Excellency Mr.Lin Songtian,former Chinese ambassador to South Africa,is succeeding me as the president of the Chinese People’s Association for Friendship with Foreign C... Dear Friends,I’m hereby informing you that His Excellency Mr.Lin Songtian,former Chinese ambassador to South Africa,is succeeding me as the president of the Chinese People’s Association for Friendship with Foreign Countries.I will conclude my45 years of service in the CPAFFC with joy and begin to enjoy my retirement life. 展开更多
关键词 service PRESIDENT cpaf
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