Whether the immunostimulatory effects of CpG-oligonucleotides （CpG ODNs） could be enhanced by the use of gold nanoparticles （Au-NP） was investigated.The CpG ODNs were modified by the Au-NP （CpG /Au-NP） and their...Whether the immunostimulatory effects of CpG-oligonucleotides （CpG ODNs） could be enhanced by the use of gold nanoparticles （Au-NP） was investigated.The CpG ODNs were modified by the Au-NP （CpG /Au-NP） and their uptake and distribution in murine N9 microglial cells were studied.The immunostimulatory effects of CpG /Au-NP on N9 cells,human B cells and plamacytoid dendritic cells （pDCs） were examined.Results showed that the uptake of CpG /Au-NP in N9 cells was much higher than that of CpG ODNs and CpG /Au-NP localized in the cytoplasm of N9 cells.The amount of TNF-α and IL12p40 in N9 cells was increased greatly by the use of Au-NP.And the amount of IL-6 in B cells and IFN-α in pDCs was also significantly increased,while the activation of B cells was not changed.These results reveal that the Au-NP can be used as a delivery media for the oligonucleotides-based therapeutics.展开更多
基金Supported by the National Natural Science Foundation of China(No.20807017)Doctoral Fund of Ministry of Education of China (No.20080487087)the Fundamental Research Funds for the Central Universities (No.2010MS091)
文摘Whether the immunostimulatory effects of CpG-oligonucleotides （CpG ODNs） could be enhanced by the use of gold nanoparticles （Au-NP） was investigated.The CpG ODNs were modified by the Au-NP （CpG /Au-NP） and their uptake and distribution in murine N9 microglial cells were studied.The immunostimulatory effects of CpG /Au-NP on N9 cells,human B cells and plamacytoid dendritic cells （pDCs） were examined.Results showed that the uptake of CpG /Au-NP in N9 cells was much higher than that of CpG ODNs and CpG /Au-NP localized in the cytoplasm of N9 cells.The amount of TNF-α and IL12p40 in N9 cells was increased greatly by the use of Au-NP.And the amount of IL-6 in B cells and IFN-α in pDCs was also significantly increased,while the activation of B cells was not changed.These results reveal that the Au-NP can be used as a delivery media for the oligonucleotides-based therapeutics.
