High-grade squamous intraepithelial lesion(HSIL)is regarded as a serious precancerous state of cervix,and it is easy to progress into cervical invasive carcinoma which highlights the importance of earlier diagnosis an...High-grade squamous intraepithelial lesion(HSIL)is regarded as a serious precancerous state of cervix,and it is easy to progress into cervical invasive carcinoma which highlights the importance of earlier diagnosis and treatment of cervical lesions.Pathologists examine the biopsied cervical epithelial tissue through a microscope.The pathological examination will take a long time and sometimes results in high inter-and intra-observer variability in outcomes.Polarization imaging techniques have broad application prospects for biomedical diagnosis such as breast,liver,colon,thyroid and so on.In our team,we have derived polarimetry feature parameters(PFPs)to characterize microstructural features in histological sections of breast tissues,and the accuracy for PFPs ranges from 0.82 to 0.91.Therefore,the aim of this paper is to distinguish automatically microstructural features between HSIL and cervical squamous cell carcinoma(CSCC)by means of polarization imaging techniques,and try to provide quantitative reference index for patho-logical diagnosis which can alleviate the workload of pathologists.Polarization images of the H&E stained histological slices were obtained by Mueller matrix microscope.The typical path-ological structure area was labeled by two experienced pathologists.Calculate the polarimetry basis parameter(PBP)statistics for this region.The PBP statistics(stat PBPs)are screened by mutual information(MI)method.The training method is based on a linear discriminant analysis(LDA)classier whichnds the most simplied linear combination from these stat PBPs and the accuracy remains constant to characterize the specic microstructural feature quantitatively in cervical squamous epithelium.We present results from 37 clinical patients with analysis regions of cervical squamous epithelium.The accuracy of PFP for recognizing HSIL and CSCC was 83.8%and 87.5%,respectively.This work demonstrates the ability of PFP to quantitatively charac-terize the cervical squamous epithelial lesions in the H&E pathological sections.Signicance:Polarization detection technology provides an effcient method for digital pathological diagnosis and points out a new way for automatic screening of pathological sections.展开更多
目的:探讨PGP9.5和CyclinD1在皮肤鳞状细胞癌(CSCC)、Bowen病中的表达及临床意义。方法:用免疫组织化学SP法检测22例CSCC,31例Bowen病及10例正常皮肤组织中PGP9.5和CyclinD1的表达情况。结果:PGP9.5与CyclinD1在CSCC的表达明显高于正常...目的:探讨PGP9.5和CyclinD1在皮肤鳞状细胞癌(CSCC)、Bowen病中的表达及临床意义。方法:用免疫组织化学SP法检测22例CSCC,31例Bowen病及10例正常皮肤组织中PGP9.5和CyclinD1的表达情况。结果:PGP9.5与CyclinD1在CSCC的表达明显高于正常皮肤组(P=0.006,P=0.00057,<0.05)和Bowen病组(P<0.05),PGP9.5与Cycl i nD1在低分化鳞癌的表达明显强于高分化鳞癌的表达(P<0.05)。在CSCC、Bowen病中,PGP9.5与CyclinD1的阳性表达存在相关性。结论:PGP9.5、Cycl i nD1的高表达和鳞癌细胞分化程度有密切关系。展开更多
目的探讨局部晚期宫颈鳞癌(cervical squamous cell carcinoma,CSCC)患者外周血和癌组织中T细胞上表达B、T细胞弱化因子(B and T lymphocyte attenuator,BTLA)的差异,初步评价其与近期疗效的关系。方法收集新疆医科大学附属肿瘤医院2017...目的探讨局部晚期宫颈鳞癌(cervical squamous cell carcinoma,CSCC)患者外周血和癌组织中T细胞上表达B、T细胞弱化因子(B and T lymphocyte attenuator,BTLA)的差异,初步评价其与近期疗效的关系。方法收集新疆医科大学附属肿瘤医院2017年1-10月收治的45例CSCC患者,采用流式细胞术检测患者外周血和癌组织中T细胞上BTLA的表达量,并结合临床资料对比分析。结果局部晚期CSCC外周血中CD4+T细胞上BTLA的表达量(60.60%)高于CSCC组织(0.57%),其差异有统计学意义(P<0.001);CSCCBTLA外周血CD8+T细胞上的表达量(32.20%)高于CSCC组织(1.04%),其差异有统计学意义(P<0.001);结节型CSCC外周血CD8+T细胞BTLA的表达量(33.40%)高于菜花型(25.60%);低分化CSCC患者癌组织CD8+T细胞上BTLA的表达量(2.99%)高于中分化(0.72%),差异君有统计学意义(P=0.011,P=0.034);BTLA在有、无恶性肿瘤家族史的CSCC患者癌组织CD4+、CD8+T细胞上的分别为表达量(7.04%、6.96%、0.54%、0.99%),差异均有统计学意义(P<0.05)。结论BTLA在局部晚期CSCC患者癌组织T细胞上的表达低于外周血,提示应用抗BTLA治疗CSCC可能会导致机体产生较严重的全身免疫相关不良反应,推测BTLA可能不是CSCC免疫治疗的理想靶点。展开更多
基金the Guangming District Economic Development Special Fund(2020R01043)。
文摘High-grade squamous intraepithelial lesion(HSIL)is regarded as a serious precancerous state of cervix,and it is easy to progress into cervical invasive carcinoma which highlights the importance of earlier diagnosis and treatment of cervical lesions.Pathologists examine the biopsied cervical epithelial tissue through a microscope.The pathological examination will take a long time and sometimes results in high inter-and intra-observer variability in outcomes.Polarization imaging techniques have broad application prospects for biomedical diagnosis such as breast,liver,colon,thyroid and so on.In our team,we have derived polarimetry feature parameters(PFPs)to characterize microstructural features in histological sections of breast tissues,and the accuracy for PFPs ranges from 0.82 to 0.91.Therefore,the aim of this paper is to distinguish automatically microstructural features between HSIL and cervical squamous cell carcinoma(CSCC)by means of polarization imaging techniques,and try to provide quantitative reference index for patho-logical diagnosis which can alleviate the workload of pathologists.Polarization images of the H&E stained histological slices were obtained by Mueller matrix microscope.The typical path-ological structure area was labeled by two experienced pathologists.Calculate the polarimetry basis parameter(PBP)statistics for this region.The PBP statistics(stat PBPs)are screened by mutual information(MI)method.The training method is based on a linear discriminant analysis(LDA)classier whichnds the most simplied linear combination from these stat PBPs and the accuracy remains constant to characterize the specic microstructural feature quantitatively in cervical squamous epithelium.We present results from 37 clinical patients with analysis regions of cervical squamous epithelium.The accuracy of PFP for recognizing HSIL and CSCC was 83.8%and 87.5%,respectively.This work demonstrates the ability of PFP to quantitatively charac-terize the cervical squamous epithelial lesions in the H&E pathological sections.Signicance:Polarization detection technology provides an effcient method for digital pathological diagnosis and points out a new way for automatic screening of pathological sections.
文摘目的:探讨PGP9.5和CyclinD1在皮肤鳞状细胞癌(CSCC)、Bowen病中的表达及临床意义。方法:用免疫组织化学SP法检测22例CSCC,31例Bowen病及10例正常皮肤组织中PGP9.5和CyclinD1的表达情况。结果:PGP9.5与CyclinD1在CSCC的表达明显高于正常皮肤组(P=0.006,P=0.00057,<0.05)和Bowen病组(P<0.05),PGP9.5与Cycl i nD1在低分化鳞癌的表达明显强于高分化鳞癌的表达(P<0.05)。在CSCC、Bowen病中,PGP9.5与CyclinD1的阳性表达存在相关性。结论:PGP9.5、Cycl i nD1的高表达和鳞癌细胞分化程度有密切关系。
文摘目的探讨局部晚期宫颈鳞癌(cervical squamous cell carcinoma,CSCC)患者外周血和癌组织中T细胞上表达B、T细胞弱化因子(B and T lymphocyte attenuator,BTLA)的差异,初步评价其与近期疗效的关系。方法收集新疆医科大学附属肿瘤医院2017年1-10月收治的45例CSCC患者,采用流式细胞术检测患者外周血和癌组织中T细胞上BTLA的表达量,并结合临床资料对比分析。结果局部晚期CSCC外周血中CD4+T细胞上BTLA的表达量(60.60%)高于CSCC组织(0.57%),其差异有统计学意义(P<0.001);CSCCBTLA外周血CD8+T细胞上的表达量(32.20%)高于CSCC组织(1.04%),其差异有统计学意义(P<0.001);结节型CSCC外周血CD8+T细胞BTLA的表达量(33.40%)高于菜花型(25.60%);低分化CSCC患者癌组织CD8+T细胞上BTLA的表达量(2.99%)高于中分化(0.72%),差异君有统计学意义(P=0.011,P=0.034);BTLA在有、无恶性肿瘤家族史的CSCC患者癌组织CD4+、CD8+T细胞上的分别为表达量(7.04%、6.96%、0.54%、0.99%),差异均有统计学意义(P<0.05)。结论BTLA在局部晚期CSCC患者癌组织T细胞上的表达低于外周血,提示应用抗BTLA治疗CSCC可能会导致机体产生较严重的全身免疫相关不良反应,推测BTLA可能不是CSCC免疫治疗的理想靶点。
