[Objective] In the Xinjiang new era from October 2012 to February 2013, agricultural and animal husbandry Co. Ltd. feedlot Yining Tuogelake village used tethered and scattered feeding methods to conduct fattening youn...[Objective] In the Xinjiang new era from October 2012 to February 2013, agricultural and animal husbandry Co. Ltd. feedlot Yining Tuogelake village used tethered and scattered feeding methods to conduct fattening young Xinjiang brown cattle experiment. [Method] The 16-month-old young Xinjiang brown bulls were se- lected totaling 68 heads, divided into scattered group and tethered group. Male claves were taken as test subjects from October to February of following year and body conformation linear evaluation were made, followed by measurement of body size indexes and Regular collection of Blood samples. [Result] In the two feeding ways, scattered group feeding improved the ADG of youth Xinjiang brown cattle bull calf （P〈0.01）, and the whole calf ADG 845.58g was significantly higher than that in tethered group at 619.11g, increasing by 36.58%; furthermore, scattered group in the December, January and February average daily weight gain was significantly higher compared with the tethered group at 13.61%, 32.32%, 53.47%, respectively. Scattered group feeding significantly increased the young calf serum phosphorus content （P〈0.01）, higher than the tethered group at 0.62%, 7.89%, 14.29% in February, December and January respectively. But the tethered feeding young cattle serum phosphorus content gradually declined. Of the two groups, male calf serum calcium content had little effects on the serum calcium with insignificant difference （P〉0.05）. Bulls by scattered feeding method was proved faster and better in the whole growth period, in terms of body height, body length, circumference, leg cir- cumference, rump length, waist high, sciatic wide, hip width basically in the contin- ued growth trend. [Conclusion] Scattered feeding can significantly improve the young Xinjiang brown cattle＇s daily gain, and accelerate the body height, heart girth and body length etc body growth. What＇s more, scattered feeding serum calcium and phosphorus content are significantly higher than tethered feeding.展开更多
Objective:To evaluate preoperative serum calcium concentration and investigate the association between calcium level and positive peritoneal cytology in endometrial carcinoma(EC).Methods:A total of 510 patients who we...Objective:To evaluate preoperative serum calcium concentration and investigate the association between calcium level and positive peritoneal cytology in endometrial carcinoma(EC).Methods:A total of 510 patients who were diagnosed with EC and had surgery were initially enrolled in this study at Peking University People's Hospital between January 2012 and December 2016.Clinical characteristics and preoperative serum calcium,albumin,carbohydrate antigen(CA)125,CA19-9,carcinoembryonic antigen(CEA)were extracted from patient records and evaluated according to postoperative peritoneal cytology.Predictive factors were assessed with Cox univariate and multivariate analyses.Factors selected from multivariate analysis results were used to build a predictive model.Results:A total of 510 patients are identified in our database and 444 patients who fulfilled inclusion and exclusion criteria are included in this study.Univariate analysis revealed that ionized calcium concentration was closely related to positive peritoneal cytology,tumor grade and lymph-vascular space invasion(LVSI).Moreover,peritoneal cytology was significantly associated with hypertension,tubal ligation,serum CA125,CA19-9,CEA and ionized calcium level.Multivariate analysis revealed that albumin-adjusted calcium level,CA125 and tubal ligation were independent predictive factors of positive peritoneal cytology(P<0.05).A combination of ionized calcium level with the other two indexes yielded significantly great area under the curve(AUC=0.824).Conclusions:This study enhanced the value of preoperative ionized calcium level.We also identified several potential biomarkers to predict positive peritoneal cytology in EC patients before surgery.展开更多
Objective Delayed graft function(DGF)and early graft loss of renal grafts are determined by the quality of the kidneys from the deceased donor.As“non-traditional”risk factors,serum biomarkers of donors,such as lipid...Objective Delayed graft function(DGF)and early graft loss of renal grafts are determined by the quality of the kidneys from the deceased donor.As“non-traditional”risk factors,serum biomarkers of donors,such as lipids and electrolytes,have drawn increasing attention due to their effects on the postoperative outcomes of renal grafts.This study aimed to examine the value of these serum biomarkers for prediction of renal graft function.Methods The present study consecutively collected 306 patients who underwent their first single kidney transplantation(KT)from adult deceased donors in our center from January 1,2018 to December 31,2019.The correlation between postoperative outcomes[DGF and abnormal serum creatinine(SCr)after 6 and 12 months]and risk factors of donors,including gender,age,body mass index(BMI),past histories,serum lipid biomarkers[cholesterol,triglyceride,high-density lipoprotein(HDL)and low-density lipoprotein(DL)],and serum electrolytes(calcium and sodium)were analyzed and evaluated.Results(1)Donor age and pre-existing hypertension were significantly correlated with the incidence rate of DGF and high SCr level(≥2 mg/dL)at 6 and 12 months after KT(P<0.05);(2)The donor’s BMI was significantly correlated with the incidence rate of DGF after KT(P<0.05);(3)For serum lipids,merely the low level of serum HDL of the donor was correlated with the reduced incidence rate of high SCr level at 12 months after KT[P<0.05,OR(95%CI):0.425(0.202–0.97)];(4)The serum calcium of the donor was associated with the reduced incidence rate of high SCr level at 6 and 12 months after KT[P<0.05,OR(95%CI):0.184(0.045–0.747)and P<0.05,OR(95%CI):0.114(0.014–0.948),respectively].Conclusion The serum HDL and calcium of the donor may serve as predictive factors for the postoperative outcomes of renal grafts after KT,in addition to the donor’s age,BMI and pre-existing hypertension.展开更多
Wistar rats were exposed to trinitrotoluene (TNT) for 6 weeks. After initiation of TNT exposure, serum phosphorylase A activities and calcium contents were assayed for every 2 weeks. Both of these 2 parameters increas...Wistar rats were exposed to trinitrotoluene (TNT) for 6 weeks. After initiation of TNT exposure, serum phosphorylase A activities and calcium contents were assayed for every 2 weeks. Both of these 2 parameters increased in rats treated with 50 and 100 mg TNT/kg b.w. at 3 intervals. Serum phosphorylase A activities and calcium contents of TNT exposure worker increased too.展开更多
Experimental and clinical studies have shown that long term anticoagulation therapy with warfarin can induce vascular calcification which is preventable by vitamin K. Osteoporosis has been shown to be associated with ...Experimental and clinical studies have shown that long term anticoagulation therapy with warfarin can induce vascular calcification which is preventable by vitamin K. Osteoporosis has been shown to be associated with vascular calcification. In the present study, we wanted to see, whether INR (International Normalized Ratio), a measure of prothrombin time, and serum calcium (corrected by albumin) correlate in laboratory data of 94 anticoagulation patients on warfarin therapy. When adjusted on age and sex, there was an inverse correlation between the two variables. Clinical relevance of this observation and explanation of lowered calcium levels in blood parallel to increase in INR-values remain to be studied further.展开更多
Hydroxyapatite has a high affinity to biological macromolecules, especially to proteins. Bovine serum proteins were extracted to be used as stablizer to prepare calcium phosphate nanoparticles . 167.7 nm and 87.7 nm p...Hydroxyapatite has a high affinity to biological macromolecules, especially to proteins. Bovine serum proteins were extracted to be used as stablizer to prepare calcium phosphate nanoparticles . 167.7 nm and 87.7 nm particles were respectively prepared by using bovine serum protein fractions at the concentration of 0. 5 mg/mL and 1.0 mg/mL. As the polysaccharide stabilized hydroxyapatite nanoparticles, the protein-stablized nanoparticles also inhibited the proliferation rate of Bel-7402 cells. It suggested that proteins could be applied to prepare calcium phosphate nanoparticles and it also has the anticaneer effect.展开更多
目的探究骨折患者初期血钙、血磷、钙磷乘积水平变化并分析其与骨折部位的相关性。方法选取2021年1月~2023年11月四川省骨科医院收治的骨折患者1049例作为研究对象,按骨折部位粗分为两组:上半身部位骨折(n=478)和下半身部位骨折(n=571)...目的探究骨折患者初期血钙、血磷、钙磷乘积水平变化并分析其与骨折部位的相关性。方法选取2021年1月~2023年11月四川省骨科医院收治的骨折患者1049例作为研究对象,按骨折部位粗分为两组:上半身部位骨折(n=478)和下半身部位骨折(n=571);细分十组:椎体骨折(n=108)、锁骨骨折(n=109)、上肢骨折(n=106)、手部骨折(n=104)、股骨颈骨折(n=103)、股骨粗隆间骨折(n=106)、髌骨骨折(n=101)、下肢骨折(n=103)、足部骨折(n=105)和其他骨折(n=104)。另选取同期健康体检人群110例作为对照组。全部患者于急诊当日和入院后24~48h两次抽取静脉血测定血钙、血磷,并计算钙磷乘积,比较三者水平变化并分析其与骨折部位的相关性。结果与对照组比较,骨折患者急诊当日和入院后24~48h,血钙(2.27±0.12 mmol/L,2.19±0.12 mmol/L vs2.35±0.10mmol/L)、血磷(1.00±0.20mmol/L,1.08±0.19mmol/L vs 1.15±0.15mmol/L)、钙磷乘积(28.10±6.00mg/dl,29.30±5.85mg/dl vs 33.41±4.87mg/dl)均降低,差异具有统计学意义(t=6.804,12.501;7.475,3.722;8.964,7.115,均P<0.01)。上、下半身骨折相比,急诊当日血钙、血磷和钙磷乘积下半身骨折低于上半身骨折(t=4.129,5.931,6.660,均P<0.01);入院后24~48h则仅钙、钙磷乘积下半身骨折低于上半身骨折(t=6.432,1.990,均P<0.05),差异具有统计学意义。沿时间轴比较,入院后24~48h与急诊当日相比,上、下半身骨折均表现出血钙降低而血磷升高(t=12.779,-5.730;16.919,-14.358),钙磷乘积则仅下半身骨折较急诊当日升高(t=-8.860),差异具有统计学意义(均P<0.01)。不同部位骨折患者入院后24~48h与急诊当日相比,除椎体骨折外,其余九组血钙均降低(t=6.233~11.349);除上肢骨折、手部骨折外,其余八组血磷升高(t=-7.770~-3.327);椎体骨折、股骨颈骨折、股骨粗隆间骨折、下肢骨折、足部骨折、其他骨折钙磷乘积升高(t=-5.819~-2.927),差异具有统计学意义(均P<0.01)。结论骨折患者急诊当日血钙、血磷和钙磷乘积均降低,24~48h后多数患者血钙持续降低,血磷和钙磷乘积逐渐回升,其水平变化程度与骨折部位有关。展开更多
基金Supported by the NationalTechnology Support Program(2011BAD47B02)~~
文摘[Objective] In the Xinjiang new era from October 2012 to February 2013, agricultural and animal husbandry Co. Ltd. feedlot Yining Tuogelake village used tethered and scattered feeding methods to conduct fattening young Xinjiang brown cattle experiment. [Method] The 16-month-old young Xinjiang brown bulls were se- lected totaling 68 heads, divided into scattered group and tethered group. Male claves were taken as test subjects from October to February of following year and body conformation linear evaluation were made, followed by measurement of body size indexes and Regular collection of Blood samples. [Result] In the two feeding ways, scattered group feeding improved the ADG of youth Xinjiang brown cattle bull calf （P〈0.01）, and the whole calf ADG 845.58g was significantly higher than that in tethered group at 619.11g, increasing by 36.58%; furthermore, scattered group in the December, January and February average daily weight gain was significantly higher compared with the tethered group at 13.61%, 32.32%, 53.47%, respectively. Scattered group feeding significantly increased the young calf serum phosphorus content （P〈0.01）, higher than the tethered group at 0.62%, 7.89%, 14.29% in February, December and January respectively. But the tethered feeding young cattle serum phosphorus content gradually declined. Of the two groups, male calf serum calcium content had little effects on the serum calcium with insignificant difference （P〉0.05）. Bulls by scattered feeding method was proved faster and better in the whole growth period, in terms of body height, body length, circumference, leg cir- cumference, rump length, waist high, sciatic wide, hip width basically in the contin- ued growth trend. [Conclusion] Scattered feeding can significantly improve the young Xinjiang brown cattle＇s daily gain, and accelerate the body height, heart girth and body length etc body growth. What＇s more, scattered feeding serum calcium and phosphorus content are significantly higher than tethered feeding.
基金supported by National Natural Science Foundation of China (No. 81874108, No. 81802607)
文摘Objective:To evaluate preoperative serum calcium concentration and investigate the association between calcium level and positive peritoneal cytology in endometrial carcinoma(EC).Methods:A total of 510 patients who were diagnosed with EC and had surgery were initially enrolled in this study at Peking University People's Hospital between January 2012 and December 2016.Clinical characteristics and preoperative serum calcium,albumin,carbohydrate antigen(CA)125,CA19-9,carcinoembryonic antigen(CEA)were extracted from patient records and evaluated according to postoperative peritoneal cytology.Predictive factors were assessed with Cox univariate and multivariate analyses.Factors selected from multivariate analysis results were used to build a predictive model.Results:A total of 510 patients are identified in our database and 444 patients who fulfilled inclusion and exclusion criteria are included in this study.Univariate analysis revealed that ionized calcium concentration was closely related to positive peritoneal cytology,tumor grade and lymph-vascular space invasion(LVSI).Moreover,peritoneal cytology was significantly associated with hypertension,tubal ligation,serum CA125,CA19-9,CEA and ionized calcium level.Multivariate analysis revealed that albumin-adjusted calcium level,CA125 and tubal ligation were independent predictive factors of positive peritoneal cytology(P<0.05).A combination of ionized calcium level with the other two indexes yielded significantly great area under the curve(AUC=0.824).Conclusions:This study enhanced the value of preoperative ionized calcium level.We also identified several potential biomarkers to predict positive peritoneal cytology in EC patients before surgery.
基金The study was supported by the Innovation Team Fund Project of Hubei Province(No.WJ2021C001)the Key Research and Development Plan of Hubei Province(No.2022BCA015).
文摘Objective Delayed graft function(DGF)and early graft loss of renal grafts are determined by the quality of the kidneys from the deceased donor.As“non-traditional”risk factors,serum biomarkers of donors,such as lipids and electrolytes,have drawn increasing attention due to their effects on the postoperative outcomes of renal grafts.This study aimed to examine the value of these serum biomarkers for prediction of renal graft function.Methods The present study consecutively collected 306 patients who underwent their first single kidney transplantation(KT)from adult deceased donors in our center from January 1,2018 to December 31,2019.The correlation between postoperative outcomes[DGF and abnormal serum creatinine(SCr)after 6 and 12 months]and risk factors of donors,including gender,age,body mass index(BMI),past histories,serum lipid biomarkers[cholesterol,triglyceride,high-density lipoprotein(HDL)and low-density lipoprotein(DL)],and serum electrolytes(calcium and sodium)were analyzed and evaluated.Results(1)Donor age and pre-existing hypertension were significantly correlated with the incidence rate of DGF and high SCr level(≥2 mg/dL)at 6 and 12 months after KT(P<0.05);(2)The donor’s BMI was significantly correlated with the incidence rate of DGF after KT(P<0.05);(3)For serum lipids,merely the low level of serum HDL of the donor was correlated with the reduced incidence rate of high SCr level at 12 months after KT[P<0.05,OR(95%CI):0.425(0.202–0.97)];(4)The serum calcium of the donor was associated with the reduced incidence rate of high SCr level at 6 and 12 months after KT[P<0.05,OR(95%CI):0.184(0.045–0.747)and P<0.05,OR(95%CI):0.114(0.014–0.948),respectively].Conclusion The serum HDL and calcium of the donor may serve as predictive factors for the postoperative outcomes of renal grafts after KT,in addition to the donor’s age,BMI and pre-existing hypertension.
文摘Wistar rats were exposed to trinitrotoluene (TNT) for 6 weeks. After initiation of TNT exposure, serum phosphorylase A activities and calcium contents were assayed for every 2 weeks. Both of these 2 parameters increased in rats treated with 50 and 100 mg TNT/kg b.w. at 3 intervals. Serum phosphorylase A activities and calcium contents of TNT exposure worker increased too.
文摘Experimental and clinical studies have shown that long term anticoagulation therapy with warfarin can induce vascular calcification which is preventable by vitamin K. Osteoporosis has been shown to be associated with vascular calcification. In the present study, we wanted to see, whether INR (International Normalized Ratio), a measure of prothrombin time, and serum calcium (corrected by albumin) correlate in laboratory data of 94 anticoagulation patients on warfarin therapy. When adjusted on age and sex, there was an inverse correlation between the two variables. Clinical relevance of this observation and explanation of lowered calcium levels in blood parallel to increase in INR-values remain to be studied further.
文摘Hydroxyapatite has a high affinity to biological macromolecules, especially to proteins. Bovine serum proteins were extracted to be used as stablizer to prepare calcium phosphate nanoparticles . 167.7 nm and 87.7 nm particles were respectively prepared by using bovine serum protein fractions at the concentration of 0. 5 mg/mL and 1.0 mg/mL. As the polysaccharide stabilized hydroxyapatite nanoparticles, the protein-stablized nanoparticles also inhibited the proliferation rate of Bel-7402 cells. It suggested that proteins could be applied to prepare calcium phosphate nanoparticles and it also has the anticaneer effect.
文摘目的探究骨折患者初期血钙、血磷、钙磷乘积水平变化并分析其与骨折部位的相关性。方法选取2021年1月~2023年11月四川省骨科医院收治的骨折患者1049例作为研究对象,按骨折部位粗分为两组:上半身部位骨折(n=478)和下半身部位骨折(n=571);细分十组:椎体骨折(n=108)、锁骨骨折(n=109)、上肢骨折(n=106)、手部骨折(n=104)、股骨颈骨折(n=103)、股骨粗隆间骨折(n=106)、髌骨骨折(n=101)、下肢骨折(n=103)、足部骨折(n=105)和其他骨折(n=104)。另选取同期健康体检人群110例作为对照组。全部患者于急诊当日和入院后24~48h两次抽取静脉血测定血钙、血磷,并计算钙磷乘积,比较三者水平变化并分析其与骨折部位的相关性。结果与对照组比较,骨折患者急诊当日和入院后24~48h,血钙(2.27±0.12 mmol/L,2.19±0.12 mmol/L vs2.35±0.10mmol/L)、血磷(1.00±0.20mmol/L,1.08±0.19mmol/L vs 1.15±0.15mmol/L)、钙磷乘积(28.10±6.00mg/dl,29.30±5.85mg/dl vs 33.41±4.87mg/dl)均降低,差异具有统计学意义(t=6.804,12.501;7.475,3.722;8.964,7.115,均P<0.01)。上、下半身骨折相比,急诊当日血钙、血磷和钙磷乘积下半身骨折低于上半身骨折(t=4.129,5.931,6.660,均P<0.01);入院后24~48h则仅钙、钙磷乘积下半身骨折低于上半身骨折(t=6.432,1.990,均P<0.05),差异具有统计学意义。沿时间轴比较,入院后24~48h与急诊当日相比,上、下半身骨折均表现出血钙降低而血磷升高(t=12.779,-5.730;16.919,-14.358),钙磷乘积则仅下半身骨折较急诊当日升高(t=-8.860),差异具有统计学意义(均P<0.01)。不同部位骨折患者入院后24~48h与急诊当日相比,除椎体骨折外,其余九组血钙均降低(t=6.233~11.349);除上肢骨折、手部骨折外,其余八组血磷升高(t=-7.770~-3.327);椎体骨折、股骨颈骨折、股骨粗隆间骨折、下肢骨折、足部骨折、其他骨折钙磷乘积升高(t=-5.819~-2.927),差异具有统计学意义(均P<0.01)。结论骨折患者急诊当日血钙、血磷和钙磷乘积均降低,24~48h后多数患者血钙持续降低,血磷和钙磷乘积逐渐回升,其水平变化程度与骨折部位有关。
